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Journal of Integrative Agriculture  2020, Vol. 19 Issue (2): 541-550    DOI: 10.1016/S2095-3119(19)62675-4
Special Issue: 植物抗病遗传合辑Plant Disease-resistance Genetics 植物细菌真菌合辑Plant Bacteria/Fungus
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Bioinformatic analysis and functional characterization of the cfem proteins in maize anthracnose fungus Colletotrichum graminicola
GONG An-dong1, 2, JING Zhong-ying1, ZHANG Kai1, TAN Qing-qun1, WANG Guo-liang1, 3, LIU Wen-de1
1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 College of Life Science, Xinyang Normal University, Xinyang 464000, P.R.China
3 Department of Plant Pathology, The Ohio State University, Columbus, Ohio 43210, USA
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Fungal secreted proteins that contain the Common in Fungal Extracellular Membrane (CFEM) domain are important for pathogenicity.  The hemibiotrophic fungus Colletotrichum graminicola causes the serious anthracnose disease of maize.  In this study, we identified 24 CgCFEM proteins in the genome of C. graminicola.  Phylogenic analysis revealed that these 24 proteins (CgCFEM1–24) can be divided into 2 clades based on the presence of the trans-membrane domain.  Sequence alignment analysis indicated that the amino acids of the CFEM domain are highly conserved and contain 8 spaced cysteines, with the exception that CgCFEM1 and CgCFEM24 lack 1 and 2 cysteines, respectively.  Ten CgCFEM proteins with a signal peptide and without the trans-membrane domain were considered as candidate effectors and, thus were selected for structural prediction and functional analyses.  The CFEM domain in the candidate effectors can form a helical-basket structure homologous to the Csa2 protein in Candida albicans, which is responsible for haem acquisition and pathogenicity.  Subcellular localization analysis revealed that these effectors accumulate in the cell membrane, nucleus, and cytosolic bodies.  Additionally, 5 effectors, CgCFEM6, 7, 8, 9 and 15, can suppress the BAX-induced programmed cell death in Nicotiana benthamiana with or without the signal peptide.  These results demonstrate that these 10 CgCFEM candidate effectors with different structures and subcellular localizations in host cells may play important roles during the pathogenic processes on maize plants.
Keywords:  CFEM domain        candidate effector        anthracnose disease        maize        Colletotrichum graminicola  
Received: 26 December 2018   Accepted:
Fund: This study was supported by the National Program for Support of Top-notch Young Professionals of China.
Corresponding Authors:  Correspondence LIU Wen-de, Tel/Fax: +86-10-62815921, E-mail:   

Cite this article: 

GONG An-dong, JING Zhong-ying, ZHANG Kai, TAN Qing-qun, WANG Guo-liang, LIU Wen-de. 2020.

Bioinformatic analysis and functional characterization of the cfem proteins in maize anthracnose fungus Colletotrichum graminicola
. Journal of Integrative Agriculture, 19(2): 541-550.

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