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Journal of Integrative Agriculture  2021, Vol. 20 Issue (1): 87-99    DOI: 10.1016/S2095-3119(20)63184-7
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Efficiency of seed bio-priming technique for healthy mungbean productivity under terminal drought stress
Hamid Nawaz1, Nazim Hussain2, Niaz Ahmed3, Haseeb-ur-Rehman2, Javaiz Alam2 
1 Department of Agronomy, The Islamia University of Bahawalpur, Bahawapur 63100, Pakistan
2 Department of Agronomy, Bahauddin Zakariya University, Multan 60000, Pakistan
3 Department of Soil Science, Bahauddin Zakariya University, Multan 60000, Pakistan
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Recently, drought-induced damaging impact in reducing the crop growth and development is drastically ranked at the top under various abiotic stresses.  And especially water stress at the reproductive growth stages termed as terminal drought has become a severe threat for mungbean productivity.  To mitigate the drought stress condition, “bio-priming” has emerged as a newly agronomic and sustainable technique in improving the mungbean production.  A 2-year field study during Kharif season 2017–2018 was conducted to investigate the efficacy of rhizobacteria seed priming in mungbean (AZRI mung-06), at Agronomic Research Area, Department of Agronomy, Bahauddin Zakariya University, Multan, Pakistan.  The experiment comprised two factors containing FA (seed treatments, control (dry seeds), hydro-priming, silicon (Si)-priming, and bio-priming (mixture strains of Pseudomonas fluorescens+Rhizobium phaseoli)) and FB (irrigation water-regimes at various growth stages including leaf formation (L), stem elongation (S)+flowering (F)+pod formation (P) containing treatments are normal irrigation (IL+S+F+P) and terminal drought stress (IF+P)).  All the treatments were arranged in randomized complete block design under factorial design and were replicated thrice.  Results indicated that the exposure of drought stress at flowering and pod formation stages hampered the morpho-physiological growth and yield of mungbean.  Nevertheless, seed priming treatments particularly bio-priming were effective in alleviating the detrimental effects of drought stress.  Bio-priming significantly increased the yield and yield components (seeds/plant, 1 000-grain weight and harvest index) of mungbean and regulated the activities/levels of antioxidants (superoxide dismutase, catalase, peroxidase, ascorbic acid, and total phenolics) under drought stress.  Compared with the control, bio-priming increased the seed yield of mungbean by 8–12% under normal as well as drought stress conditions during both years of study.  Bio-priming also improved the nutrient uptake behavior followed by Si- and hydro-priming treatments under terminal drought stress.  The study emphasized the effectiveness of bio-priming as dual seed treatment method may be helpful for vigorous germination of mungbean production along with plant tolerance against terminal drought stress.  Among the various treatments, plants treated with bio-priming technique compensated the grain yield due to having strong antioxidant defense system and better nutrient uptake behaviour under terminal drought stress.  Economic analysis also concluded that bio-priming is the easiest, cost-effective, friendly, and sustainable approach for the maximization of the mungbean production.
Keywords:  bio-priming       antioxidant activities        drought       nutrients uptake        mungbean   
Received: 09 October 2019   Accepted:
Corresponding Authors:  Correspondence Hamid Nawaz, E-mail:; Nazim Hussain, E-mail:   

Cite this article: 

Hamid NAWAZ, Nazim HUSSAIN, Niaz AHMED, Haseeb-ur-REHMAN, Javaiz ALAM. 2021. Efficiency of seed bio-priming technique for healthy mungbean productivity under terminal drought stress. Journal of Integrative Agriculture, 20(1): 87-99.

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