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Journal of Integrative Agriculture  2018, Vol. 17 Issue (11): 2458-2466    DOI: 10.1016/S2095-3119(18)61967-7
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Intergenic spacer 1 (IGS1) polymorphism map: A marker for the initial classification of cultivated Lentinula edodes strains in China
SONG Xiao-xia*, ZHAO Yan*, SONG Chun-yan, LI Chuan-hua, CHEN Ming-jie, HUANG Jian-chun, TAN Qi
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R.China
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China is currently the world’s leading producer of Lentinula edodes and owns many cultivated strains of this species.  This study was performed in order to investigate intergenic spacer 1 (IGS1) polymorphism and classification among 49 popular cultivated strains.  The great majority of the 49 strains possessed two different IGS1 sequences, with distinct lengths and homologies.  Based on the length and homology of the IGS1 sequences of the 49 strains, the strains were classified into two groups: A and B.  Group A was subdivided into six subgroups.  Forty-seven strains were homozygous or heterozygous among these six subgroups in group A, Cr01 was heterozygous between A and B, and Guangxiang 9 was homozygous in group B.  An IGS1 polymorphism map of each cultivated L. edodes strain is reported for the first time and could be used as a marker for the initial classification and management of cultivated L. edodes strains in China. 
Keywords:  Lentinula edodes        strain        intergenic spacer        polymorphism        genotype  
Received: 10 November 2017   Accepted:
Fund: This work was supported by the earmarked fund for China Agriculture Research System (CARS-20), the Youth Talent Development Plan of Shanghai Municipal Agricultural System, China (20160113) and the Agriculture Research System of Shanghai, China (201809).
Corresponding Authors:  Correspondence CHEN Ming-jie, Tel: +86-21-62200747, Fax: +86-21-62201337, E-mail:    
About author:  SONG Xiao-xia, E-mail:; ZHAO Yan, E-mail:; * These authors contributed equally to this study.

Cite this article: 

SONG Xiao-xia, ZHAO Yan, SONG Chun-yan, LI Chuan-hua, CHEN Ming-jie, HUANG Jian-chun, TAN Qi. 2018. Intergenic spacer 1 (IGS1) polymorphism map: A marker for the initial classification of cultivated Lentinula edodes strains in China. Journal of Integrative Agriculture, 17(11): 2458-2466.

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