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Special Issue:
植物抗病遗传合辑Plant Disease-resistance Genetics
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| Genetic diversity and population structure of Commelina communis in China based on simple sequence repeat markers |
YANG Juan1, 2, YU Hai-yan2, LI Xiang-ju2, DONG Jin-gao1 |
1 Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, College of Life Science, Hebei Agricultural University, Baoding 071001, P.R.China
2 Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China |
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Abstract Commelina communis (Asiatic dayflower) is a troublesome weed in China. Genetic variation of 46 C. communis populations from different collection sites in our country was investigated using 12 simple sequence repeat (SSR) primer pairs. Polymorphism analysis results showed high level of genetic diversity among these populations. The alleles (bands) were amplified by these primer pairs. The polymorphic proportion was 18.25%, and the average polymorphism information content was 0.1330. The highest effective number of alleles was 1.9915 at locus YP33, and the lowest value was 1.0000 at both loci YP25 and YP31. C. communis showed major average observed heterozygosity value (0.8655) than that of average expected heterozygosity (0.1330). C. communis populations were divided into three groups on the basis of unweighted pair-group method with arithmetic mean cluster analysis (Dice genetic similarity coefficient=0.772) and genetic structure analysis (K=3), and a principal coordinate analysis. The results of this study further illustrated that C. communis populations contained abundant genetic information, and the 12 SSR markers could detect the microsatellite loci of C. communis genomic DNA. These results might indicate that C. communis maintains high genetic diversity among different populations.
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Received: 29 December 2017
Accepted:
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| Fund: This study was funded by the National Key Research and Development Program of China (2016YFD0300701) and the earmarked fund for China Agriculture Research System (CARS-25). |
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Corresponding Authors:
Correspondence LI Xiang-ju, Tel: +86-10-62813309, E-mail: xjli@ippcaas.cn; DONG Jin-gao, Tel: +86-312-7528266, E-mail: dongjingao@126.com
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| About author: YANG Juan, E-mail: yangjuan018@126.com; |
Cite this article:
YANG Juan, YU Hai-yan, LI Xiang-ju, DONG Jin-gao.
2018.
Genetic diversity and population structure of Commelina communis in China based on simple sequence repeat markers. Journal of Integrative Agriculture, 17(10): 2292-2301.
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