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Journal of Integrative Agriculture  2018, Vol. 17 Issue (09): 2042-2053    DOI: 10.1016/S2095-3119(17)61891-4
Special Issue: 植物细菌真菌合辑Plant Bacteria/Fungus
Plant Protection Advanced Online Publication | Current Issue | Archive | Adv Search |
Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted
ZHENG Na1*, ZHANG Liu-ping1*, GE Feng-yong1, HUANG Wen-kun1, KONG Ling-an1, PENG De-liang1, LIU Shi-ming1, 2  
1 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 College of Plant Protection, Hunan Agricultural University, Changsha 410128, P.R.China
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摘要  Received  13 December, 2017    Accepted  17 January, 2018

Fusarium is usually thought to cause soybean root rot, which results in a large quantity of annual yield loss in soybean production, by its secretions including Fusarium toxins and cell wall degrading enzymes, but not by the conidia themselves that do not underlie any virulence so far.  Here we report that the conidia of one Fusarium solani isolate are able to be virulent to soybean and make soybean seedlings wilted alone.  We isolated them from the wilted plants in a soybean-production field and molecularly identified 17 Fusarium isolates through phylogenetic analysis.  Of them, except for one isolate that showed diversity of virulence to different soybeans (virulent to one soybean whereas avirulent to another soybean), the others were all virulent to the two tested soybeans: both conidia cultures and secretions could make soybean seedlings wilted at 5 days post infection, and their virulence had dosage effects that only conidia cultures of at least 5×106 conidia mL–1 could show virulence to soybean; however, the sole conidia of the F. solani isolate #4 also exhibited virulence to soybean and could make soybean seedlings wilted.  Finally, we developed the specific cleaved amplified polymorphic sequences (CAPS) markers to easily differentiate Fusarium isolates.  The isolate #4 in this work will likely be used to investigate the new mechanism of virulence of Fusarium to soybean.     
Keywords:  Fusarium        soybean root rot        conidia        secretions        virulence        cleaved amplified polymorphic sequences (CAPS) marker  
Received: 31 December 2017   Accepted:
Fund: This work was financially supported by the Innovation Program and Youth Elite Program of Chinese Academy of Agricultural Sciences.
Corresponding Authors:  Correspondence LIU Shi-ming, E-mail:   
About author:  ZHENG Na, E-mail:; ZHANG Liu-ping, E-mail:; * These authors contributed equally to this study

Cite this article: 

ZHENG Na, ZHANG Liu-ping, GE Feng-yong, HUANG Wen-kun, KONG Ling-an, PENG De-liang, LIU Shi-ming. 2018. Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted. Journal of Integrative Agriculture, 17(09): 2042-2053.

Bai G H, Plattner R, Desjardins A, Kolb F, McIntosh R A. 2001. Resistance to Fusarium head blight and deoxynivalenol accumulation in wheat. Plant Breeding, 120, 1–6.
Brar H K, Swaminathan S, Bhattacharyya M K. 2011. The Fusarium virguliforme toxin FvTox1 causes foliar sudden death syndrome-like symptoms in soybean. Molecular Plant-Microbe Interactions, 24, 1179–1188.
Brito N, Espino J J, González C. 2006. The endo-beta-1,4-xylanase xyn11A is required for virulence in Botrytis cinerea. Molecular Plant-Microbe Interactions, 19, 25–32.
Brown N A, Urban M, van de Meene A M, Hammond-Kosack K E. 2010. The infection biology of Fusarium graminearum: Defining the pathways of spikelet to spikelet colonization in wheat ears. Fungal Biology, 114, 555–571.
Bruns T D, Taylor J W. 2003. Fungal molecular systematics. Annual Review of Ecology Systematics, 22, 525–564.
Bushell W R, Hazen B E, Pritsch C. 2003. Histology and physiology of Fusarium head blight. In: Leonard K J, Bushell W R, eds., Fusarium Head Blight of Wheat and Barley. St. Paul, APS Press, USA. pp. 44–83.
Cooper R M, Longman D, Campbell A, Henry M, Lees P E. 1998. Enzymic adaptation of cereal pathogens to the monocotyledonous primary wall. Physiological and Molecular Plant Pathology, 32, 33–47.
Cui L, Yin W, Tang Q. 2010. Distribution, pathotypes, and metalaxyl sensitivity of Phytophthora sojae from Heilongjiang and Fujian provinces in China. Plant Disease, 94, 881–884.
Del-Prado R, Cubas P, Lumbsch H T, Divakar P K, Blanco O, de Paz G A, Molina M C, Crespo A. 2010. Genetic distances within and among species in monophyletic lineages of Parmeliaceae (Ascomycota) as a tool for taxon delimitation. Molecular Phylogenetics and Evolution, 56, 125–133.
Fang Z D. 1998. Phytoremediation Research Methods. 3rd ed. China Agriculture Press, Beijing, China. pp. 137–140. (in Chinese)
Gardes M, Bruns T D. 1993. ITS primers with enhanced specificity for basidiomycetes - Application to the identification of mycorrhizae and rusts. Molecular Ecology, 2, 113–118.
Guadet J, Julien J, Lafay J F, Brygoo Y. 1989. Phylogeny of some Fusarium species, as determined by large-subunit rRNA sequence comparison. Molecular Biology and Evolution, 6, 227–242.
Islam K T, Bond J P, Fakhoury A M. 2017. FvSTR1, a striatin orthologue in Fusarium virguliforme, is required for asexual development and virulence. Applied Microbiology and Biotechnology, 101, 6431–6445.
Jayasinghe C K, Wijayaratne S C, Fernando T H. 2004. Characterization of cell wall degrading enzymes of Thanatephorus cucumeris. Mycopathologia, 157, 73–79.
Koenning S R, Wrather J A. 2010. Suppression of soybean yield potential in the continental United States from plant diseases estimated from 2006 to 2009. Plant Health Progress.
Kwon S, Anderson A J. 2002. Genes for multicopper proteins and laccase activity: Common features in plant-associated Fusarium isolates. Canadian Journal of Botany, 80, 563–570.
Lightfoot D A. 2015. Two decades of molecular marker-assisted breeding for resistance to soybean sudden death syndrome. Crop Science, 55, 1460–1484.
Liu S M, Li W, Dai LY. 2016. Progresses in research on the resistance of soybean to Phytophthora root rot caused by Phytophthora sojae. Chinese Soybean Science, 35, 320–329. (in Chinese)
Liu Z. 1992. The study of soybean root rot. Chinese Journal of Oil Crop Science, 1, 42–44. (in Chinese)
Ma Z, Zhu L, Song T, Wang Y, Zhang Q, Xia Y, Qiu M, Lin Y, Li H, Kong L, Fang Y, Ye W, Wang Y, Dong S, Zheng X, Tyler B M, Wang Y. 2017. A paralogous decoy protects Phytophthora sojae apoplastic effector PsXEG1 from a host inhibitor. Science, 355, 710–714.
Naito S, Mohamad D, Nasution A, Purwanti H. 1993. Soil-borne diseases and ecology of pathogens on soybean roots in Indonesia. Japan Agricultural Research, 26, 247–247.
Nelson B D, Hansen J M, Windels C E, Helms T C. 1997. Reaction of soybean cultivars to isolates of Fusarium solani from the Red River Valley. Plant Disease, 81, 664–668.
Paccanaro M C, Sella L, Castiglioni C, Giacomello F, Martínez-Rocha A L, D’Ovidio R, Schäfer W, Favaron F. 2017. Synergistic effect of different plant cell wall-degrading enzymes is important for virulence of Fusarium graminearum. Molecular Plant-Microbe Interactions, 30, 886–895.
Pan F J, McLaughlin N B, Yu Q. 2010. Responses of soil nematode community structure to different long-term fertilizer strategies in the soybean phase of a soybean-wheat-corn rotation. European Journal of Soil Biology, 46, 105–111.
Qiu X Y, Tang Z P, Zhang M, Jing H Y. 2011. Research on the isolation method of single spore of most plant pathogenic fungi. Journal of Anhui Agriculture, 39, 5263–5264. (in Chinese)
Schoch C L, Seifert K A, Huhndorf S, Robert V, Spouge J L, Levesque C A, Chen W, Bolchacova E, Voigt K, Crous P W, Miller A N, Wingfield M J, Aime M C, An K D, Bai F Y, Barreto R W, Begerow D, Bergeron M J, Blackwell M, Boekhout T, et al. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi. Proceedings of National Academy of Science of the United States of America, 109, 6241–6246.
Tai L M, Xu Y L, Yan F Y. 2006. Effects of Fusarium oxysporium toxin on the ultrastructure of soybean radicle tissue. Acta Phytopathology Sinica, 36, 512–516. (in Chinese)
Tooley P W, Carras M M, Falkenstein K F. 1996. Relationships among group IV Phytophthora species inferred by restriction an analysis of the ITS2 region. Journal of Phytopathology, 144, 363–369.
White T J, Bruns T D, Lee S B, Taylor J W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M A, Gelfand D H, Sninsky J J, White T J, eds., PCR Protocols - A Guide to Methods and Applications. Academic Press, San Diego, USA. pp. 315–322.
Wu M, Zhang H, Li X, Zhang Y. 2008. Soil fungistasis and its relations to soil microbial composition and diversity: A case study of a series of soils with different fungistasis. Journal of Environmental Sciences, 20, 871–877.
Wu X, Blake S, Sleper D A, Shannon J G, Cregan P, Nguyen H T. 2009. QTL, additive and epistatic effects for SCN resistance in PI 437654. Theoretical and Applied Genetics, 118, 1093–1105.
Zakaria G, Lockwood J L. 1981. Fusarium spp. from soybean roots. Phytopathology, 71, 157–161.
Zhang J Z, Xue A G, Zhang H J, Nagasawa A E, Tambong J T. 2010. Response of soybean cultivars to root rot caused by Fusarium species. Canadian Journal of Plant Science, 90, 767–776.
Zhang S, Xu P, Wu J. 2010. Races of Phytophthora sojae and their virulence on soybean cultivars in Heilongjiang, China. Plant Disease, 94, 87–91.
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