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Special Issue:
植物细菌真菌合辑Plant Bacteria/Fungus
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| Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted |
ZHENG Na1*, ZHANG Liu-ping1*, GE Feng-yong1, HUANG Wen-kun1, KONG Ling-an1, PENG De-liang1, LIU Shi-ming1, 2 |
1 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2 College of Plant Protection, Hunan Agricultural University, Changsha 410128, P.R.China |
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摘要 Received 13 December, 2017 Accepted 17 January, 2018
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Abstract Fusarium is usually thought to cause soybean root rot, which results in a large quantity of annual yield loss in soybean production, by its secretions including Fusarium toxins and cell wall degrading enzymes, but not by the conidia themselves that do not underlie any virulence so far. Here we report that the conidia of one Fusarium solani isolate are able to be virulent to soybean and make soybean seedlings wilted alone. We isolated them from the wilted plants in a soybean-production field and molecularly identified 17 Fusarium isolates through phylogenetic analysis. Of them, except for one isolate that showed diversity of virulence to different soybeans (virulent to one soybean whereas avirulent to another soybean), the others were all virulent to the two tested soybeans: both conidia cultures and secretions could make soybean seedlings wilted at 5 days post infection, and their virulence had dosage effects that only conidia cultures of at least 5×106 conidia mL–1 could show virulence to soybean; however, the sole conidia of the F. solani isolate #4 also exhibited virulence to soybean and could make soybean seedlings wilted. Finally, we developed the specific cleaved amplified polymorphic sequences (CAPS) markers to easily differentiate Fusarium isolates. The isolate #4 in this work will likely be used to investigate the new mechanism of virulence of Fusarium to soybean.
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Received: 31 December 2017
Accepted:
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| Fund: This work was financially supported by the Innovation Program and Youth Elite Program of Chinese Academy of Agricultural Sciences. |
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Corresponding Authors:
Correspondence LIU Shi-ming, E-mail: smliuhn@yahoo.com
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| About author: ZHENG Na, E-mail: 476211775@qq.com; ZHANG Liu-ping, E-mail: Liupingz2013@163.com;
* These authors contributed equally to this study |
Cite this article:
ZHENG Na, ZHANG Liu-ping, GE Feng-yong, HUANG Wen-kun, KONG Ling-an, PENG De-liang, LIU Shi-ming.
2018.
Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted. Journal of Integrative Agriculture, 17(09): 2042-2053.
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