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Journal of Integrative Agriculture  2016, Vol. 15 Issue (11): 2550-2555    DOI: 10.1016/S2095-3119(16)61400-4
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Effect of temperature on the development of sclerotia in Villosiclava virens
FAN Lin-lin1, YONG Ming-li1, LI Dan-yang1, LIU Yi-jia1, LAI Chao-hui2, CHEN Hong-ming2, CHENG Fang-min3, HU Dong-wei1
1 The State Key Laboratory of Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou 310058, P.R.China
2 Plant Protection Station of Xiangshan County, Xiangshan 315700, P.R.China
3 Crop Science Institute, Zhejiang University, Hangzhou 310058, P.R.China
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Abstract      The sclerotia of Villosiclava virens were found commonly in high altitude and the temperate regions, where the temperatures are relatively low in rice filling stage. To make sure if low temperature induce the sclerotial formation in V. virens, the inoculated rice panicles in laboratory and the diseased rice panicles cut from paddy fields were treated under different temperatures. The results showed that 3 days of night temperature at 15°C were enough to induce the sclerotial formation. The low temperature was much more effective for young balls with intact membranes. After appearance of chlamydospores on the ball surfaces, the sclerotium could not differentiate anymore. The sclerotia began to differentiate below the chlamydospore layer and gradually grew onto the ball surfaces. This suggests that low temperature in the early development stage of false smut balls is an important factor to induce the sclerotial differentiation, and rice cultivars with long growth periods are able to produce more sclerotium-bearing balls, which will produce mass of spores in paddy field in the coming year.
Keywords:  Villosiclava virens        Ustilaginoidea virens        rice false smut        sclerotial formation        temperature  
Received: 25 January 2016   Accepted:

This work was financially supported by the National Natural Science Foundation of China (31271999) and the Special Fund for Agro-scientific Research in the Public Interest, China (200903039-5). Dr. Tanaka of Ishikawa Prefectural University, Japan and Dr. Ikegami of Faculty of Agriculture, Gifu University, Gifu, Japan, provided some references.

Corresponding Authors:  HU Dong-wei, Tel/Fax: +86-571-88982409, E-mail:   
About author:  FAN Lin-lin, E-mail:;

Cite this article: 

FAN Lin-lin, YONG Ming-li, LI Dan-yang, LIU Yi-jia, LAI Chao-hui, CHEN Hong-ming, CHENG Fang-min, HU Dong-wei. 2016. Effect of temperature on the development of sclerotia in Villosiclava virens. Journal of Integrative Agriculture, 15(11): 2550-2555.

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