Please wait a minute...
Journal of Integrative Agriculture  2018, Vol. 17 Issue (04): 786-795    DOI: 10.1016/S2095-3119(17)61716-7
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Evaluation of a new method for quantification of heat tolerance in different wheat cultivars
LI Qiang, WANG Zheng-rui, LI Ding, WEI Jian-wei, QIAO Wen-chen, MENG Xiang-hai, SUN Shu-luan, LI Hui-min, ZHAO Ming-hui, CHEN Xiu-min, ZHAO Feng-wu
Dryland Farming Institute, Hebei Academy of Agricultural and Forestry Sciences/Key Laboratory of Crop Drought Tolerance Research of Hebei Province, Hengshui 053000, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Abstract  Heat stress seriously affects wheat production in many regions of the world.  At present, heat tolerance research remains one of the least understood fields in wheat genetics and breeding and there is a lack of effective methods to quantify heat stress and heat tolerance in different wheat cultivars.  The objective of this study was to use various wheat cultivars to evaluate stress intensity (δ) and a new method for quantification of heat tolerance and compare this technique with three other currently utilized methods.  This new parameter for heat tolerance quantification is referred to as the heat tolerance index (HTI) and is an indicator of both yield potential and yield stability.  Heat treatments were applied in a controlled setting when anthesis had been reached for 80% of the wheat.  The stress intensity evaluation indicated heat shock was the main factor associated with kernel weight reduction while grain yield reduction was mainly associated with chronic high temperature.  The methods evaluation showed that a temperature difference of 5°C from natural temperatures was a suitable heat treatment to compare to the untreated controls.  HTI was positively correlated with yield under heat stress (r=0.8657, δ2010=0.15, in 2009–2010; r=0.8418, δ2011=0.20, in 2010–2011; P<0.01), and negatively correlated with yield reduction rate (r=–0.8344, in 2009–2010; r=–0.7158, in 2010–2011; P<0.01).  The results of this study validated the use of HTI and temperature difference control for quantifying wheat heat tolerance that included the yield potential and the stability of different wheat cultivars under heat stress.  Additionally, 10 wheat cultivars showed high HTI and should be further tested for their heat confirming characteristics for use in wheat heat tolerance breeding.
Keywords:  wheat breeding        heat tolerance quantification        HTI        temperature difference controlling        stress intensity  
Received: 21 March 2017   Accepted:

Work reported here was partially supported by the Generation Challenge Program, CIMMYT (International Maize and Wheat Improvement Center) (GCP, G7010.02.01), the earmarked fund for China Agriculture Research System (CARS-3-2-3), and the National Key Technology R&D Program of China (2016YFD0100502, 2016YFD0300407).

Corresponding Authors:  Correspondence ZHAO Feng-wu, Tel/Fax: +86-318-7920312, E-mail:   
About author:  LI Qiang, Tel/Fax: +86-318-7920312, E-mail:;

Cite this article: 

LI Qiang, WANG Zheng-rui, LI Ding, WEI Jian-wei, QIAO Wen-chen, MENG Xiang-hai, SUN Shu-luan, LI Hui-min, ZHAO Ming-hui, CHEN Xiu-min, ZHAO Feng-wu. 2018. Evaluation of a new method for quantification of heat tolerance in different wheat cultivars. Journal of Integrative Agriculture, 17(04): 786-795.

Ahmad R, Qadir S, Ahmad N, Shah K H. 2003. Yield potential and stability of nine wheat varieties under water stress conditions. International Journal of Agriculture & Biology, 5, 7–9.

Allakhverdiev S I, Kreslavski V D, Klimov V V, Los D A, Carpentier R, Mohanty P. 2008. Heat stress: An overview of molecular responses in photosynthesis. Photosynthesis Research, 98, 541–550.

Amani I, Fischer R A, Reynolds M P. 1996. Canopy temperature depression associated with yield of irrigated spring wheat cultivars in a hot climate. Journal of Agronomy and Crop Science, 176, 119–129.

Blum A. 1973. Components analysis of yield responses to drought of sorghum hybrids. Experimental Agriculture, 9, 159–167.

Blum A, Klueva N, Nguyen H T. 2001. Wheat cellular thermotolerance is related to yield under heat stress. Euphytica, 117, 117–123.

Blum A, Shipler L, Golan G, Mayer J. 1989. Yield stability and canopy temperature of wheat genotypes under drought stress. Field Crops Research, 22, 289–296.

Borrell A K, Hammer G L, Douglas A C L. 2000. Does maintaining green leaf area in sorghum improve yield under drought? I. Leaf growth and senescence. Crop Science, 40, 1026–1037.

Chinoy J J. 1947. Correlation between yield of wheat and temperature during ripening of grain. Nature (London), 159, 442–444.

Clarke J M, Depaw R M, Tounley-Smith T F. 1992. Evaluation of methods for quantification of drought tolerance in wheat. Crop Science, 32, 723–727.

Demirevska-Kepova K, Holzer R, Simova-Stoilova L, Feller U. 2005. Heat stress effects on ribulose-1,5-bisphosphate carboxylase/oxygenase, Rubisco binding protein and Rubisco activase in wheat leaves. Biologia Plantarum, 49, 521–525.

Farooq M, Bramley H, Palta J A, Siddique K H M. 2011. Heat stress in wheat during reproductive and grain filling phases. Critical Reviews in Plant Sciences, 30, 491–507.

Figueiredo I C R D, Pinto C A B P, Ribeiro G H M R. 2015. Efficiency of selection in early generations of potato families with a view toward heat tolerance. Crop Breeding and Applied Biotechnology, 15, 210–217.

Fischer R A, Maurer R. 1978. Drought tolerance in spring wheat cultivars. I: Grain yield response. Australian Journal of Agricultural Research, 29, 897–912.

Fokar M, Blum A, Nguyen H T. 1998. Heat tolerance in spring wheat. II. Gram filling. Euphytica, 104, 9–15.

Gooding M J, Ellis R H, Shewry P R, Schofield J D. 2003. Effects of restricted water availability and increased temperature on the grain filling, drying and quality of winter wheat. Journal of Cereal Science, 37, 295–309.

Hoffmann S, Debreczeni K, Hoffmann B, Nagy E. 2006. Grain yield and baking quality of wheat as affected by crop year and plant nutrition. Cereal Research Communications, 34, 473–476.

Joshi A K, Chand R, Arun B, Singh R P, Ortiz R. 2007a. Breeding crops for reduced-tillage management in the intensive, rice-wheat systems of South Asia. Euphytica, 153, 135–151.

Joshi A K, Kumari M, Singh V P, Reddy C M, Kumar S, Rane J, Chand R. 2007b. Staygreen trait: Variation, inheritance and its association with spot blotch tolerance in spring wheat (Triticum aestivum L.). Euphytica, 153, 59–71.

Kumari M Y, Pudake R N, Singh V P, Joshi A K. 2013. Association of staygreen trait with canopy temperature depression and yield traits under terminal heat stress in wheat (Triticum aestivum L.). Euphytica, 190, 87–97.

Lazar M D, Salisbury C D, Worrall W D. 1995. Variation in drought susceptibility among closely related wheat lines. Field Crops Research, 41, 147–153.

Lobell D, Ortiz-Monasterio I. 2007. Impacts of day versus night temperature on spring wheat yields: A comparison of empirical and CERES model predictions in three locations. Agronomy Journal, 99, 469–477.

Mason R E, Mondal S, Beecher F W. 2010. QTL associated with heat susceptibility index in wheat (Triticum aestivum L.) under short-term reproductive stage heat stress. Euphytica, 174, 423–436.

Rane J, Nagarajan S. 2004. High temperature index - for field evaluation of heat tolerance in wheat varieties. Agricultural Systems, 79, 243–255.

Reynolds M P, Balota M, Delgado M I B, Amani I, Fischer R A. 1994. Physiological and morphological traits associated with spring wheat yield under hot, irrigated conditions. Australian Journal of Plant Physiology, 21, 717–730.

Reynolds M P, Singh R P, Ibrahim A, Ageeb O A A, Larque-Saavedra A, Quick J S. 1998. Evaluating hysiological traits to complement empirical selection for wheat in warm environments. Euphytica, 100, 85–94.

Sharkey T D. 2005. Effect of moderate heat stress on photosynthesis: Importance of thylakoid reactions, rubisco deactivation, reactive oxygen species and thermotolerance provided by isoprene. Plant Cell Environment, 28, 269–277.

Tewari A K, Tripathy B C. 1998. Temperature-stress-induced impairment of chlorophyll biosynthetic reactions in cucumber and wheat. Plant Physiology, 117, 851–858.

Tewolde H, Fernandez C J, Erickson C A. 2006. Wheat cultivars adapted to post-heading high temperature stress. Journal of Agronomy and Crop Science, 192, 111–120.

Thomas H, Howarth C J. 2000. Five ways to staygreen. Journal of Experimental Botany, 51, 329–337.

Towill L E, Mazur P. 1974. Studies on the reduction of 2,3,5-triphenyl tetrazolium chloride as a viability assay for plant tissue culture. Canadian Journal of Botany, 53, 1097–1102.

Villegas D, Garcia L F, Rharrabti Y, Martos V, Royo C. 2007. Morphological traits above the flag leaf node as indicators of drought susceptibility index in durum wheat. Journal of Agronomy and Crop Science, 193, 103–116.

Wanous M K, Miller F R, Rosenow D T. 1991. Evaluation of visual rating scales for green leaf retention in sorghum. Crop Science, 31, 1691–1694.

Wardlaw I F, Blumenthal C, Larroque O, Wrigley C W. 2002. Contrasting effects of chronic heat stress and heat shock on kernel weight and flour quality in wheat. Functional Plant Biology, 29, 25–34.

Wiegand C L, Cuellar J A. 1981. Duration of grain filling and kernel weight of wheat as effected by temperature. Crop Science, 21, 95–101.

Xu W, Rosenow D T, Nguyen H T. 2000. Stay-green trait in grain sorghum: Relationship between visual rating and leaf chlorophyll concentration. Plant Breeding, 119, 365–367.
No related articles found!
No Suggested Reading articles found!