|
|
|
|
|
| Effective remediation of aged HMW-PAHs polluted agricultural soil by the combination of Fusarium sp. and smooth bromegrass (Bromus inermis Leyss.) |
| SHI Wei1, ZHANG Xue-na1, JIA Hai-bin1, FENG Sheng-dong1, YANG Zhi-xin1, ZHAO Ou-ya1, LI Yu-ling2 |
1 Key Laboratory for Farmland Eco-Environment of Hebei Province/College of Resource and Environmental Sciences, Agricultural University of Hebei, Baoding 0710001, P.R.China
2 College of Forestry, Agricultural University of Hebei, Baoding 0710001, P.R.China |
|
|
|
|
Abstract Fusarium sp. strain ZH-H2 is capable to degrade high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs), smooth bromegrass (Bromus inermis Leyss.) can also degrade 4- to 6-ring PAHs. Pot experiments were conducted to investigate how bromegrass and different inoculum sizes of ZH-H2 clean up HMW-PAHs in agricultural soil derived from a coal mine area. The results showed that, compared with control, different sizes of inocula of ZH-H2 effectively degraded HMW-PAHs, with removal rates of 19.01, 34.25 and 29.26% for 4-, 5- and 6-ring PAHs in the treatment with 1.0 g kg–1 ZH-H2 incubation after 90 d. After 5 mon of cultivation, bromegrass reached degradation rate of these compounds by 12.66, 36.26 and 36.24%, respectively. By adding strain ZH-H2 to bromegrass, HMW-PAHs degradation was further improved up to 4.24 times greater than bromegrass (W), in addition to the degradation rate of Bbf decrease. For removal rates of both 5- and 6-ring PAHs, addition of 0.5 g kg–1 Fusarium ZH-H2 to pots with bromegrass performed better than addition of 0.1 g kg–1, while the highest concentration of 1.0 g kg–1 Fusarium ZH-H2 did not further improve degradation. Degradation of 4-ring PAHs showed no significant difference among different ZH-H2 incubations with bromegrass treatments. We found that the degradation rates of 4-, 5- and 6-ring PAHs in all treatments are significantly correlated in a positive, linear manner with activity of lignin peroxidase (LiP) (r=0.8065, 0.9350 and 0.9165, respectively), while degradation of 5- and 6-ring PAHs is correlated to polyphenoloxidase (PPO) activity (r=0.7577 and 07806). Our findings suggest that the combination of Fusarium sp. ZH-H2 and bromegrass offers a suitable alternative for phytoremediation of aged PAH-contaminated soil in coal mining areas, with a recommended inoculation size of 0.5 g Fusarium sp. ZH-H2 per kg soil.
|
|
Received: 21 November 2015
Online: 19 April 2016
Accepted:
|
| Fund: This study was supported by the National High-Tech R&D Program of China (863 Program) (2012AA101403), the Educational Commission of Hebei Province of China (Z2013058), the Human Resources Department of Hebei Province of China (2013–2016 Project), the Educational Commission of Hebei Province of China (ZD2013013). |
|
Corresponding Authors:
YANG Zhi-xin, Tel: +86-312-7528236, E-mail: yangzhixin@126.com
|
| About author: SHI Wei, Mobile: +86-15933568252, E-mail: 649985324@qq.com |
Cite this article:
SHI Wei, ZHANG Xue-na, JIA Hai-bin, FENG Sheng-dong, YANG Zhi-xin, ZHAO Ou-ya, LI Yu-ling.
2017.
Effective remediation of aged HMW-PAHs polluted agricultural soil by the combination of Fusarium sp. and smooth bromegrass (Bromus inermis Leyss.). Journal of Integrative Agriculture, 16(01): 199-209.
|
| Alkorta I, Garbisu C. 2001. Phytoremediation of organic contaminants in soils. Bioresource Technology, 79, 273–276.Arora D S, Gill P K. 2001. Effects of various media and supplements on laccase production by some white rot fungi. Bioresource Technology, 77, 89–91.Boonchan S, Britz M L, Stanley G A. 2000. Degradation and mineralization of high-molecular-weight polycyclic aromatic hydrocarbons by defined fungal-bacterial cocultures. Applied and Environmental Microbiology, 66, 1007–1019.Carl E C. 1992. Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation, 3, 351–368.Chen J, Chen S. 2005. Removal of polycyclic aromatic hydrocarbons by low density polyethylene from liquid model and roasted meat. Food Chemistry, 90, 461–469.Ding K Q, Luo Y N, Liu S L. 2008. Ecotoxicity effect of polycyclic aromatic hydrocarbons on wheat growth. Journal of Nanjing Institute of Technology, 6, 52–56. Fan S X, Li P J, He N, Ren W X, Zhang H R, Xu H X. 2007. Research of phytoremediation on contaminated soil with polycyclic aromatic hydrocarbons (PAHs). Journal of Agro-Environment Science, 26, 2007–2013. (in Chinese)Gaboriau H, Saada A. 2001. Influence of heavy organic pollutants of anthrop icorigin on PAH retention by kaolinite. Chemosphere, 44, 1633–1639.Guan S Y. 1986. Soil Enzymes and Research Methods. China Agricultural Science Press, Beijing, China. (in Chinese)Hughes J B, Beckles D M, Chandra S D, Ward C H. 1997. Utilization of bioremediation processes for the treatment of PAH-contaminated sediments. Journal of Industrial Microbiology and Biotechnology, 18, 152–160.Liu R, Xiao N, Wei S, Zhao L, An J. 2014. Rhizosphere effects of PAH-contaminated soil phytoremediation using a special plant named Fire Phoenix. Science of the Total Environment, 473–474, 350–358.Liu S L, Luo Y M, Ding K Q, Li H, Wu L H, Xing W Q. 2004. Enhanced phytoremediation of benzo[a]pyrene contaminated soil with arbuscular mycorrhizal fungi. Acta Pedologica Sinica, 41, 336–342. (in Chinese)Liu W W, Yin R, Lin X G, Zhang J, Chen X M, Li Z S, Li X Z, Xiao Y P. 2010. Interaction of phytoremediation-microorganism to remediation of aged polycyclic aromatic hydrocarbons (PAHs) polluted soils. Soils, 42, 800–806. (in Chinese)Liu W, Sun J, Ding L, Luo Y, Chen M, Tang C. 2013. Rhizobacteria (Pseudomonas sp. SB) assist phytoremediation of oily-sludge-contaminated soil by tall fescue (Testuca arundinacea L.). Plant Soil, 371, 533–542.Lu Y F, Lu M. 2015. Remediation of PAH-contaminated soil by the combination of tall fescue, arbuscular mycorrhizal fungus and epigeic earthworms. Journal of Hazardous Materials, 285, 535–541.Potin O, Rafin C, Veignie E. 2004. Bioremediation of an aged polycyclic aromatic hydrocarbons (PAHs)-contaminated soil by filamentous fungi isolated from the soil. International Biodeterioration & Biodegradation, 54, 45–52.Shen Y Y, Teng Y, Luo Y M, Sun M M, Fu D Q, Sheng X F, Li Z G. 2011. Remediation efficiency of several legumes and grasses in PAH-contaminated soils. Soils, 43, 253–257. (in Chinese)Shree N S, Rudra D T. 2007. Environmental Bioremediation Technologies. Springer, Berlin.Sun T H, Song Y F, Xu H X, Zhang H R, Yang G F. 1999. Plant bioremediation of PAHs and mineral oil contaminated soil. Chinese Journal of Applied Ecology, 10, 225–229. (in Chinese)Tien M, Kick T K. 1984. Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase. Proceedings of the National Academy of Sciences of the United States of America, 81, 2280–2284.Wang C P, Sun H W, Li J M, Li Y M, Zhang Q M. 2009. Enzyme activities during degradation of polycyclic aromatic hydrocarbons by white rot fungus Phanerochaete chrysosporium in soils, Chemosphere, 77, 733–738.Wang W, Feng S D, Yang Z X, Chang R X, Li Y L, Wang X M. 2015. Effects of sludge/soil ratio on the remediation of PAH-contaminated sludge by smooth bromegrass. Acta Prataculturae Sinica, 24, 148–160. (in Chinese)Wilson S C, Jones K C. 1993. Bioremediation of soil contaminated with polynuclear aromatic hydrocarbons (PAHs): A review. Environmental Pollution, 81, 229–249.Xiao N, Liu R, Jin C X, Dai Y Y. 2015. Efficiency of five ornamental plant species in the phytoremediation of polycyclic aromatic hydrocarbon (PAH)-contaminated soil. Ecological Engineering, 75, 384–391.Yang T, Lin X, Hu J L, Zhang J, Lv J L, Wang J H, Li X Z. 2009. Effects of arbuscular mycorrhizal fungi on phytoremediation of PAHs-contaminated soil by Medicago sativa and Lolium muhiflorum. Journal of Ecology and Rural Environment, 25, 72–76. (in Chinese)Yao L F, Teng Y, Liu F, Wu Y G, Li Z G, Luo Y M. 2014. Influence of trichoderma reesei and Rhizobium meliloti on phytoremediation of PAH-contaminated soil by alfalfa. Ecology and Environmental Sciences, 23, 890–896. (in Chinese)Zhang J, Xian G L, Li X Z, Yin R. 2010. Interactive effect of spent mushroom compost and rhamnolipids to enhance the effeciency of alfalfa remediation of aged PAHs contaminated soil. Environmental Science, 31, 2431–2438. (in Chinese)Zhang X H, Wang X Y. 2010. Determination of 16 polycyclic aromatic hydrocarbons in soils by gas chromatography-mass spectrometry. Rock and Mineral Analysis, 31, 535–538. (in Chinese)Zhao O Y, Feng S D, Jia H B, Zhang X N, Shi W ,Wang W, Yang Z X, Li Y L. 2016. Biodegradation of high molecular weight polycyclic aromatic hydrocarbons mixture by a newly isolated Fusarium sp. and co-metabolic degradation with starch. Polycyclic Aromatic Compounds, doi: 10.1080/10406638.2016.1143847Zhao O Y, Feng S D, Shi W, Wang W, Yang Z X, Li C G, Li Y L. 2015. A method for assessing the ecological risks due to the polycyclic aromatic hydrocarbons in the farming soil near coal mine area. Journal of Safety and Environment, 15, 352–358. (in Chinese)Zhu H, Wang H W, Du W, Dai C C. 2014. Potential role of the endopytic fungus laccase rLACB3 in the bioremediation of peanut continuous cropping soil. Soil Biology and Biochemistry, 33, 1920–1927 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
