|
|
|
|
|
| Cloning, bioinformatics and transcriptional analysis of caffeoylcoenzyme A 3-O-methyltransferase in switchgrass under abiotic stress |
| LIU Si-jia, HUANG Yan-hua, HE Chang-jiu, FANG Cheng, ZHANG Yun-wei |
1、Department of Grassland Science, College of Animal Science and Technology, China Agricultural University/National Energy
R&D Center for Biomass (NECB), Beijing 100193, P.R.China
2、Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture/National Engineering Laboratory for Animal Breeding,
College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.China |
|
|
|
摘要 Genes encoding enzymes involved in the lignin biosynthesis through phenylpropanoid pathway were not only associated with the lignin content, but also related to the abiotic stress resistances. As far as the production of liquid biofuels and cultivation within the marginal land are concerned, switchgrass could be the better candidate to determine the relationship between lower lignin content and physiological function under stress. Caffeoyl-coenzyme A 3-O-methyltransferase (CCoAOMT) is a key enzyme for the methylation reaction of lignin biosynthesis. For this purpose, we cloned a CCoAOMT gene from switchgrass and identified its expression patterns under abiotic stresses. The full-length CCoAOMT gene, designated PvCCoAOMT (GenBank accession no. KF041775), was 1 005-bp in length, has an opening reading frame of 777 nucleotides encoding a 258-amino acid protein. The deduced amino acid sequence of PvCCoAOMT shared a high degree of similarity (up to 98%) with CCoAOMTs from Panicum virgatum allele (BAO20881), Sorghum bicolor (XP002436550) and Zea mays (NP001131288). Using quantitative real-time PCR (qRT-PCR), the significant upregulation of PvCCoAOMT was observed in stem tissues at a later stage (24 h) after drought treatment, with the transcript level increasing 33-fold compared that of the controls. Moderate and insignificant inductions of PvCCoAOMT were also observed in both stems and leaves during the later stages after cold (48 h in stems, 12 h in leaves) and mechanical wounding (48 h in stems, 12 h in leaves) treatments, respectively. Our results showed the different expression patterns of PvCCoAOMT in drought, cold and mechanical wounding stresses. PvCCoAOMT can be highly induced by drought and cold stresses, which indicates that it may play a role in plant abiotic stress resistance, particularly in the regulation of drought and cold resistance.
Abstract Genes encoding enzymes involved in the lignin biosynthesis through phenylpropanoid pathway were not only associated with the lignin content, but also related to the abiotic stress resistances. As far as the production of liquid biofuels and cultivation within the marginal land are concerned, switchgrass could be the better candidate to determine the relationship between lower lignin content and physiological function under stress. Caffeoyl-coenzyme A 3-O-methyltransferase (CCoAOMT) is a key enzyme for the methylation reaction of lignin biosynthesis. For this purpose, we cloned a CCoAOMT gene from switchgrass and identified its expression patterns under abiotic stresses. The full-length CCoAOMT gene, designated PvCCoAOMT (GenBank accession no. KF041775), was 1 005-bp in length, has an opening reading frame of 777 nucleotides encoding a 258-amino acid protein. The deduced amino acid sequence of PvCCoAOMT shared a high degree of similarity (up to 98%) with CCoAOMTs from Panicum virgatum allele (BAO20881), Sorghum bicolor (XP002436550) and Zea mays (NP001131288). Using quantitative real-time PCR (qRT-PCR), the significant upregulation of PvCCoAOMT was observed in stem tissues at a later stage (24 h) after drought treatment, with the transcript level increasing 33-fold compared that of the controls. Moderate and insignificant inductions of PvCCoAOMT were also observed in both stems and leaves during the later stages after cold (48 h in stems, 12 h in leaves) and mechanical wounding (48 h in stems, 12 h in leaves) treatments, respectively. Our results showed the different expression patterns of PvCCoAOMT in drought, cold and mechanical wounding stresses. PvCCoAOMT can be highly induced by drought and cold stresses, which indicates that it may play a role in plant abiotic stress resistance, particularly in the regulation of drought and cold resistance.
|
|
Received: 18 November 2014
Accepted: 09 March 2016
|
| Fund: Funding for this work was provided by the Ministry of Science and Technology, China (2012AA101801, 2014BAD23B00), the National Natural Science Foundation of China (31272493), and China Agricultural University (2014FG062). |
|
Corresponding Authors:
ZHANG Yun-wei, E-mail: zywei@126.com
E-mail: zywei@126.com
|
| About author: LIU Si-jia, E-mail: liusj8899@163.com; |
Cite this article:
LIU Si-jia, HUANG Yan-hua, HE Chang-jiu, FANG Cheng, ZHANG Yun-wei.
2016.
Cloning, bioinformatics and transcriptional analysis of caffeoylcoenzyme A 3-O-methyltransferase in switchgrass under abiotic stress. Journal of Integrative Agriculture, 15(3): 636-649.
|
| Arbona V, Manzi M, Ollas C, Gomez-Cadenas A. 2013.Metabolomics as a tool to investigate abiotic stresstolerance in plants. International Journal of MolecularSciences, 14, 4885-4911Barrière Y, Riboulet C, Méchin V, Maltese S, Pichon M, CardinalA, Lapierre C, Lubberstedt T, Martinant J P. 2007. Geneticsand genomics of lignification in grass cell walls based onmaize as model species. Genes Genomes Genomics, 1,133-156Baxter H L, Stewart Jr C N. 2013. Effects of altered ligninbiosynthesis on phenylpropanoid metabolism and plantstress. Biofuels, 4, 635-650Bianchi M W, Damerval C, Vartanian N. 2002. Identificationof proteins regulated by cross-talk between drought andhormone pathways in Arabidopsis wild-type and auxininsensitivemutants, axr1 and axr2. Functional Plant Biology,29, 55-61Chen C, Meyermans H, Burggraeve B, De Rycke R M, Inoue K,De Vleesschauwer V, Steenackers M, Van Montagu M C,Engler G J, Boerjan W A. 2000. Cell-specific and conditionalexpression of caffeoyl-coenzyme A 3-O-methyltransferasein poplar. Plant Physiology, 123, 853-868Chen F, Dixon R A. 2007. Lignin modification improvesfermentable sugar yields for biofuel production. NatureBiotechnology, 25, 759-761Cheng H, Li L, Xu F, Cheng S, Cao F, Wang Y, Yuan H, JiangD, Wu C. 2013. Expression patterns of a cinnamyl alcoholdehydrogenase gene involved in lignin biosynthesis andenvironmental stress in Ginkgo biloba. Molecular BiologyReports, 40, 707-721Combet C, Blanchet C, Geourjon C, Deleage G. 2000. NPS@:network protein sequence analysis. Trends in BiochemicalSciences, 25, 147-150Costa P, Bahrman N, Frigerio J M, Kremer A, Plomion C. 1998.Water-deficit-responsive proteins in maritime pine. PlantMolecular Biology, 38, 587-596Cui S, Huang F, Wang J, Ma X, Cheng Y, Liu J. 2005. Aproteomic analysis of cold stress responses in riceseedlings. Proteomics, 5, 3162-3172Czechowski T, Bari R P, Stitt M, Scheible W R, Udvardi M K.2004. Real-time RT-PCR profiling of over 1400 Arabidopsistranscription factors: Unprecedented sensitivity revealsnovel root-and shoot-specific genes. The Plant Journal,38, 366-379Ding S Y, Liu Y S, Zeng Y, Himmel M E, Baker J O, Bayer EA. 2012. How does plant cell wall nanoscale architecturecorrelate with enzymatic digestibility? Science, 338,1055-1060Domon J M, Baldwin L, Acket S, Caudeville E, Arnoult S, ZubH, Gillet F, Lejeune-Henaut I, Brancourt-Hulmel M, PellouxJ, Rayon C. 2013. Cell wall compositional modificationsof Miscanthus ecotypes in response to cold acclimation.Phytochemistry, 85, 51-61Escamilla-Trevino L L, Shen H, Uppalapati S R, Ray T, Tang Y,Hernandez T, Yin Y, Xu Y, Dixon R A. 2010. Switchgrass(Panicum virgatum) possesses a divergent family ofcinnamoyl CoA reductases with distinct biochemicalproperties. The New Phytologist, 185, 143-155Fan L, Linker R, Gepstein S, Tanimoto E, Yamamoto R,Neumann P M. 2006. Progressive inhibition by water deficitof cell wall extensibility and growth along the elongationzone of maize roots is related to increased lignin metabolismand progressive stelar accumulation of wall phenolics. PlantPhysiology, 140, 603-612Ford C W, Morrison I, Wilson J. 1979. Temperature effects onlignin, hemicellulose and cellulose in tropical and temperategrasses. Crop and Pasture Science, 30, 621-633Fossdal C G, Nagy N E, Hietala A M, Kvaalen H, Slimestad R,Woodward S, Solheim H. 2012. Indications of heightenedconstitutive or primed host response affecting the ligninpathway transcripts and phenolics in mature Norway spruceclones. Tree Physiology, 32, 1137-1147Frei M. 2013. Lignin: Characterization of a multifaceted cropcomponent. The Scientific World Journal. [2013-09-24].http://dx.doi.org/10.1155/2013/436517Fu C, Mielenz J R, Xiao X, Ge Y, Hamilton C Y, RodriguezJr M, Chen F, Foston M, Ragauskas A, Bouton J, DixonR A, Wang Z Y. 2011a. Genetic manipulation of ligninreduces recalcitrance and improves ethanol productionfrom switchgrass. Proceedings of the National Academy ofSciences of the United States of America, 108, 3803-3808Fu C, Xiao X, Xi Y, Ge Y, Chen F, Bouton J, Dixon R A,Wang Z Y. 2011b. Downregulation of cinnamyl alcoholdehydrogenase (CAD) leads to improved saccharificationefficiency in switchgrass. BioEnergy Research, 4, 153-164Gasteiger E, Hoogland C, Gattiker A, Wilkins M R, Appel RD, Bairoch A. 2005. Protein identification and analysistools on the ExPASy server. In: The Proteomics ProtocolsHandbook. Springer, Totowa, NJ. pp. 571-607Ghosh R, Choi B S, Jeong M J, Bae D W, Shin S C, Park S U,Lim H S, Kim J, Bae H. 2012. Comparative transcriptionalanalysis of caffeoyl-coenzyme A 3-O-methyltransferasefrom ’Hibiscus cannabinus’ L., during developmentalstages in various tissues and stress regulation. Plant OmicsJournal, 5, 184-193Guo D, Chen F, Inoue K, Blount J W, Dixon R A. 2001a.Downregulation of caffeic acid 3-O-methyltransferaseand caffeoyl CoA 3-O-methyltransferase in transgenicalfalfa: Impacts on lignin structure and implications for thebiosynthesis of G and S lignin. The Plant Cell Online, 13,73-88Guo D, Chen F, Wheeler J, Winder J, Selman S, Peterson M,Dixon R A. 2001b. Improvement of in-rumen digestibilityof alfalfa forage by genetic manipulation of ligninO-methyltransferases. Transgenic Research, 10, 457-464Hisano H, Nandakumar R, Wang Z Y. 2009. Geneticmodification of lignin biosynthesis for improved biofuelproduction. In Vitro Cellular & Developmental Biology (Plant), 45, 306-313Hong Y Y, Huang Z G, Yi T Y. 2011. Cloning and bioinformaticsanalysis of CCoAOMT from Triarrhena lutarioriparia var.lutarioriparia. Journal of Central South University of Forestry& Tchnology, 31, 94-99 (in Chinese)Hu Y, Li W C, Xu Y, Li G, Liao Y, Fu F L. 2009. Differentialexpression of candidate genes for lignin biosynthesis underdrought stress in maize leaves. Journal of Applied Genetics,50, 213-223Imin N, Kerim T, Weinman J J, Rolfe B G. 2006. Low temperaturetreatment at the young microspore stage induces proteinchanges in rice anthers. Molecular & Cellular Proteomics,5, 274-292Janska A, Aprile A, Zamecnik J, Cattivelli L, Ovesna J. 2011.Transcriptional responses of winter barley to cold indicatenucleosome remodelling as a specific feature of crowntissues. Functional & Integrative Genomics, 11, 307-325Joshi C P, Chiang V L. 1998. Conserved sequence motifs in plantS-adenosyl-L-methionine-dependent methyltransferases.Plant Molecular Biology, 37, 663-674Keshwani D R, Cheng J J. 2009. Switchgrass for bioethanoland other value-added applications: A review. BioresourceTechnology, 100, 1515-1523Kim Y J, Kim D G, Lee S H, Lee I. 2006. Wound-inducedexpression of the ferulate 5-hydroxylase gene inCamptotheca acuminata. Biochimica et Biophysica Acta(BBA) (General Subjects), 1760, 182-190Kühnl T, Koch U, Heller W, Wellmann E. 1989. Elicitorinduced S-adenosyl-L-methionine: Caffeoyl-CoA3-O-methyltransferase from carrot cell suspension cultures.Plant Science, 60, 21-25Kumar S, Tamura K, Nei M. 2004. MEGA3: integrated softwarefor molecular evolutionary genetics analysis and sequencealignment. Briefings in Bioinformatics, 5, 150-163Li C M, Wang Y, Yu W X. 2011. Dynamic changes of phenoliccompound contents in leaf and bark of poplar duringautumn temperature drop. Journal of Forestry Research,22, 481-485Li L, Osakabe Y, Joshi C P, Chiang V L. 1999. Secondaryxylem-specific expression of caffeoyl-coenzyme A3-O-methyltransferase plays an important role in themethylation pathway associated with lignin biosynthesis inloblolly pine. Plant Molecular Biology, 40, 555-565Martz F, Maury S, Pinçon G, Legrand M. 1998. cDNA cloning,substrate specificity and expression study of tobaccocaffeoyl-CoA 3-O-methyltransferase, a lignin biosyntheticenzyme. Plant Molecular Biology, 36, 427-437Maury S, Geoffroy P, Legrand M. 1999. TobaccoO-methyltransferases involved in phenylpropanoidmetabolism. The different caffeoyl-coenzyme A/5-hydroxyferuloyl-coenzyme A 3/5-O-methyltransferase andcaffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferaseclasses have distinct substrate specificities and expressionpatterns. Plant Physiology, 121, 215-224Michel B E, Kaufmann M R. 1973. The osmotic potential ofpolyethylene glycol 6000. Plant Physiology, 51, 914-916Moore K, Moser L E, Vogel K P, Waller S S, Johnson B,Pedersen J F. 1991. Describing and quantifying growthstages of perennial forage grasses. Agronomy Journal,83, 1073-1077Moura J C, Bonine C A, De Oliveira Fernandes Viana J,Dornelas M C, Mazzafera P. 2010. Abiotic and bioticstresses and changes in the lignin content and compositionin plants. Journal of Integrative Plant Biology, 52, 360-376Neutelings G. 2011. Lignin variability in plant cell walls:contribution of new models. Plant Science, 181, 379-386Nielsen M, Lundegaard C, Lund O, Petersen T N. 2010.CPHmodels-3.0-remote homology modeling usingstructure-guided sequence profiles. Nucleic AcidsResearch, 38, W567-W581.Olenichenko N, Zagoskina N. 2005. Response of winterwheat to cold: Production of phenolic compoundsand L-phenylalanine ammonia lyase activity. AppliedBiochemistry and Microbiology, 41, 600-603Pakusch A E, Kneusel R E, Matern U. 1989. S-adenosyl-L - m e t h i o n i n e : Trans- c a ffeoyl-coenzyme A3-O-methyltransferase from elicitor-treated parsleycell suspension cultures. Archives of Biochemistry andBiophysics, 271, 488-494Petersen T N, Brunak S, Von Heijne G, Nielsen H. 2011. SignalP4.0: discriminating signal peptides from transmembraneregions. Nature Methods, 8, 785-786Ralph S G, Yueh H, Friedmann M, Aeschliman D, Zeznik J A,Nelson C C, Butterfield Y S N, Kirkpatrick R, Liu J, JonesS J M, Marra M A, Douglas C J, Ritland K, Bohlmann J.2006. Conifer defence against insects: microarray geneexpression profiling of sitka spruce (Picea sitchensis)induced by mechanical wounding or feeding by sprucebudworms (Choristoneura occidentalis) or white pineweevils (Pissodes strobi) reveals large-scale changes ofthe host transcriptome. Plant, Cell and Environment, 29,1545-1570Senthil-Kumar M, Hema R, Suryachandra T R, Ramegowda HV, Gopalakrishna R, Rama N, Udayakumar M, Mysore KS. 2010. Functional characterization of three water deficitstress-induced genes in tobacco and Arabidopsis: Anapproach based on gene down regulation. Plant Physiologyand Biochemistry, 48, 35-44Shen H, Mazarei M, Hisano H, Escamilla-Trevino L, Fu C, Pu Y,Rudis M R, Tang Y, Xiao X, Jackson L. 2013a. A genomicsapproach to deciphering lignin biosynthesis in switchgrass.The Plant Cell Online, 25, 4342-4361Shen H, Poovaiah C R, Ziebell A, Tschaplinski T J, PattathilS, Gjersing E, Engle N L, Katahira R, Pu Y, Sykes R.2013b. Enhanced characteristics of genetically modifiedswitchgrass (Panicum virgatum L.) for high biofuelproduction. Biotechnology for Biofuels, 6, 71.Solecka D, Boudet A M, Kacperska A. 1999. Phenylpropanoidand anthocyanin changes in low-temperature treated winteroilseed rape leaves. Plant Physiology and Biochemistry,37, 491-496Tiimonen H, Haggman H, Tsai C J, Chiang V, Aronen T. 2007. The seasonal activity and the effect of mechanical bendingand wounding on the PtCOMT promoter in Betula pendulaRoth. Plant Cell Reports, 26, 1205-1214Tschaplinski T J, Standaert R F, Engle N L, Martin M Z, SanghaA K, Parks J M, Smith J C, Samuel R, Jiang N, Pu Y. 2012.Down-regulation of the caffeic acid O-methyltransferasegene in switchgrass reveals a novel monolignol analog.Biotechnology for Biofuels, 5, 71.Vanholme R, Morreel K, Ralph J, Boerjan W. 2008. Ligninengineering. Current Opinion in Plant Biology, 11, 278-285Vincent D, Lapierre C, Pollet B, Cornic G, Negroni L, Zivy M.2005. Water deficits affect caffeate O-methyltransferase,lignification, and related enzymes in maize leaves. Aproteomic investigation. Plant Physiology, 137, 949-960Vogt T. 2010. Phenylpropanoid biosynthesis. Molecular Plant,3, 2-20Wagner A, Tobimatsu Y, Phillips L, Flint H, Torr K, DonaldsonL, Pears L, Ralph J. 2011. CCoAOMT suppression modifieslignin composition in Pinus radiata. The Plant Journal, 67,119-129Wei H U I, Dhanaraj A L, Arora R, Rowland L J, Fu Y N, SunL I. 2006. Identification of cold acclimation-responsiveRhododendron genes for lipid metabolism, membranetransport and lignin biosynthesis: Importance of moderatelyabundant ESTs in genomic studies. Plant, Cell andEnvironment, 29, 558-570Weng J K, Chapple C. 2010. The origin and evolution of ligninbiosynthesis. The New Phytologist, 187, 273-285Xu B, Escamilla-Trevino L L, Sathitsuksanoh N, Shen Z, ShenH, Zhang Y H, Dixon R A, Zhao B. 2011. Silencing of4-coumarate: Coenzyme A ligase in switchgrass leads toreduced lignin content and improved fermentable sugaryields for biofuel production. The New Phytologist, 192,611-625Yamaguchi M, Valliyodan B, Zhang J, Lenoble M E, Yu O,Rogers E E, Nguyen H T, Sharp R E. 2010. Regulation ofgrowth response to water stress in the soybean primary root.I. Proteomic analysis reveals region-specific regulation ofphenylpropanoid metabolism and control of free iron in theelongation zone. Plant, Cell & Environment, 33, 223-243Yang L, Wang C, Guo W, Li X, Lu M, Yu C. 2006. Differentialexpression of cell wall related genes in the elongation zoneof rice roots under water deficit. Russian Journal of PlantPhysiology, 53, 390-395Ye Z H, Kneusel R E, Matern U, Varner J E. 1994. An alternativemethylation pathway in lignin biosynthesis in Zinnia. ThePlant Cell Online, 6, 1427-1439Yoshimura K, Masuda A, Kuwano M, Yokota A, Akashi K. 2008.Programmed proteome response for drought avoidance/tolerance in the root of a C(3) xerophyte (wild watermelon)under water deficits. Plant & Cell Physiology, 49, 226-241Yu C S, Chen Y C, Lu C H, Hwang J K. 2006. Predictionof protein subcellular localization. Proteins (Structure,Function, and Bioinformatics), 64, 643-651Zhao H, Sheng Q, Lü S, Wang T, Song Y. 2004. Characterizationof three rice CCoAOMT genes. Chinese Science Bulletin,49, 1602-1606Zhong R, Morrison W H, Himmelsbach D S, Poole F L,Ye Z H. 2000. Essential role of caffeoyl coenzyme AO-methyltransferase in lignin biosynthesis in woody poplarplants. Plant Physiology, 124, 563-578 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
