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| Changes in phenolic content, composition and antioxidant activity of blood oranges during cold and on-tree storage |
| ZHAO Ji-chun1*, AO Miao1*, HE Xiao-qin1, LI Wei-zhou1, DENG Li-li1, 2, ZENG Kai-fang1, 2, MING Jian1, 2 |
1 College of Food Science, Southwest University, Chongqing 400715, P.R.China
2 Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, P.R.China
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摘要
柑橘类水果富含酚类化合物，对人体具有多种健康益处。然而，很少有研究关注这类水果在采后贮藏过程中酚类化合物的变化。本研究对低温贮藏和留树保鲜12周的Tarocco血橙的酚类含量、成分和抗氧化活性进行了动态监测，并通过评估苯丙烷途径相关酶活性和基因表达探讨了血橙酚类化合物变化机制。结果表明，黄烷酮是血橙中主要酚类化合物，两种贮藏方式主要通过促进酚酸的积累从而提高总酚含量，并在第12周达到最大值。留树保鲜血橙的酚类含量和抗氧化性要高于低温贮藏血橙。此外，苯丙烷途径相关酶活性和基因表达结果显示，贮藏期间血橙中酚类物质的积累与苯丙烷途径的激活具有高度相关性。以上结果表明，从酚类化合物的角度来看，留树保鲜储存是延长血橙供应期的一种潜在有效方式。
Abstract Citrus fruits are rich in phenolic compounds that possess several health benefits. However, few studies have focused on the changes in phenolic compounds in citrus fruits during postharvest storage. This study dynamically monitored the phenolic content, components and antioxidant activity of ‘Tarocco’ blood oranges during a period of 12-week cold storage and on-tree storage, respectively. We investigated the alteration mechanism of phenolic compounds in blood oranges by evaluating phenylpropanoid pathway-related enzyme activities and gene expression. Results showed that flavanones were the main phenolic compounds in blood oranges. Both storage methods mainly stimulated the accumulation of phenolic acids to improve total phenolic content, which reached the maximum at week 12. Nonetheless, blood oranges had a higher phenolic content and antioxidant activity under on-tree storage than cold storage. Furthermore, the enzyme activities and gene expression of the phenylpropanoid pathway demonstrated that the accumulation of phenolics in blood oranges during storage was highly related to the activation of the phenylpropanoid pathway. These results demonstrate that on-tree storage is a potential approach for extending the supply period of blood orange from the perspective of phenolic compounds.
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Received: 22 April 2022
Accepted: 05 July 2022
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| Fund:
This work was supported by National Key R&D Program of China (2018YFD0401301), and Chongqing postgraduate scientific research innovation project (CYS18120).
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| About author:
ZHAO Ji-chun, E-mail: jichunzhao@swu.edu.cn; AO Miao, E-mail: 940698142@qq.com; Correspondence MING Jian, Tel: +86-23-68251298, Fax: +86-23-68251947, E-mail: mingjian1972@163.com, food_mj@swu.edu.cn
* These authors contributed equally to this study. |
Cite this article:
ZHAO Ji-chun, AO Miao, HE Xiao-qin, LI Wei-zhou, DENG Li-li, ZENG Kai-fang, MING Jian.
2022.
Changes in phenolic content, composition and antioxidant activity of blood oranges during cold and on-tree storage. Journal of Integrative Agriculture, 21(12): 3669-3683.
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