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Journal of Integrative Agriculture  2017, Vol. 16 Issue (11): 2623-2631    DOI: 10.1016/S2095-3119(16)61584-8
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Effects of thiamine on Trichothecium and Alternaria rots of muskmelon fruit and the possible mechanisms involved
GE Yong-hong, LI Can-ying, LÜ Jing-yi, ZHU Dan-shi   
College of Food Science and Engineering, Bohai University/Food Safety Key Laboratory of Liaoning Province/National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou 121013, P.R.China
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Abstract  The effects of thiamine against pink and black spot rots caused by Trichothecium roseum and Alternaria alternata and modulation on the metabolism of reactive oxygen species (ROS) and phenylpropanoid pathway were investigated in this paper.  In vitro test indicated that thiamine significantly inhibited mycelia growth and spore germination of T. roseum and A. alternata.  Thiamine at 100 mmol L–1 effectively inhibited lesion development of muskmelon fruit inoculated with T. roseum or A. alternata, enhanced production rate of O2-. and H2O2 content, activities of catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) in muskmelon fruit.  Thiamine also affect phenylpropanoid pathway in muskmelon fruit by increasing the activities of phenylalanine ammonia lyase (PAL) and peroxidase (POD), the content of total phenolic compounds, flavonoids and lignin.  These results suggest that the effects of thiamine on pink and black spot rots in muskmelon fruits are associated with its direct fungitoxic against the pathogens and the modulation of O2-. and H2O2 production, eliminating enzymes and phenylpropanoid pathway.
Keywords:   induced resistance        thiamine        muskmelon fruit        antimicrobial activity        phenylpropanoid pathway  
Received: 10 September 2016   Accepted:

This study was supported by the National Natural Science Foundation of China (31401554), and the Doctoral Initial Funding of Bohai University, China (bsqd201405).

Corresponding Authors:  Correspondence GE Yong-hong, E-mail:    

Cite this article: 

GE Yong-hong, LI Can-ying, Lü Jing-yi, ZHU Dan-shi. 2017. Effects of thiamine on Trichothecium and Alternaria rots of muskmelon fruit and the possible mechanisms involved. Journal of Integrative Agriculture, 16(11): 2623-2631.

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