|
|
|
The variation of NAD+-SDH gene in mutant white-fleshed loquat |
LI Jing1, 2, 3, WANG Yong-qing1, CHEN Dong2, 3, TU Mei-yan2, 3, XIE Hong-jiang2, 3, JIANG Guo-liang2, 3, LIU Jia2, 3, SUN Shu-xia2, 3 |
1 College of Horticulture, Sichuan Agricultural University, Ya’an 611130, P.R.China
2 Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R.China
3 Southwestern Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement, Ministry of Agriculture, Chengdu 610066, P.R.China |
|
|
Abstract Loquat (Eriobotrya japonica Lindl.) can be divided into yellow- and white-fleshed cultivars by flesh color. However, a Dongting loquat mutant, which involved bud sport and growing white-fleshed fruit in the central region of the trunk (as wild loquat bears yellow-fleshed fruits naturally), was discovered in the preliminary study. The study cloned the coding sequence (CDS) of NAD+-dependent sorbitol dehydrogenase (NAD+-SDH ) gene from the selected materials of mutant loquat, wild loquat and other nine loquat cultivars/accessions, and found that the CDS of NAD+-SDH gene from the mutant loquat, other than the rest two types of materials, had three single nucleotide polymorphisms (SNPs) loci; in addition, the amino acid encoded at variation loci changed accordingly. NAD+-SDH plays an active role in converting sorbitol into fructose in loquat cultivars. For the mutant white-fleshed loquat, the activity of NAD+-SDH rises first and then drops, the sorbitol content decreases steadily, and its fructose content is higher than that in wild loquat from coloration to maturation stage. As demonstrated by the real-time fluorescence quantification PCR analysis, the expression level of NAD+-SDH gene at maturation stage is about 5-fold lower than wild type. It may be assumed that, the three SNPs loci might lead to excessive conversion of sorbitol into fructose under the catalytic action of NAD+-SDH of white-fleshed mutant loquat at maturation stage, resulting in the increase of fructose content and reduced expression abundance of mRNA after transcription. Besides, NAD+-SDH gene may be related to flesh color and carbohydrate variation of white-fleshed mutant loquat.
|
Received: 31 July 2015
Accepted:
|
Fund: This study was supported by the Key Laboratory Program of the Ministry of Agriculture of China (2013JCYJ-004) and its supplementary items (2015JSCX-036, 2015LWJJ-010); the Program of Modern Agriculture Technology Innovation and Demonstration of Provincial Finance Department, China (2014CXSF-015). |
Corresponding Authors:
SUN Shu-xia, E-mail: sshuxia@163.com; WANG Yong-qing, E-mail: yqw14@sicau.edu.cn
|
Cite this article:
LI Jing, WANG Yong-qing, CHEN Dong, TU Mei-yan, XIE Hong-jiang, JIANG Guo-liang, LIU Jia, SUN Shu-xia.
2016.
The variation of NAD+-SDH gene in mutant white-fleshed loquat. Journal of Integrative Agriculture, 15(8): 1744-1750.
|
Akiko I, Havama H, Kashimura Y. 2005. Partial cloning and expression analysis of genes encoding NAD-dependent sorbitol dehvdrogenase in pear bud during flower bud formation. Scientia Horticulturae, 103, 413–420.Amoros A, Zapata P, Pretel M T, Botella M A, Ser-Rang M. 2003. Physico-chemical and physiological changes during fruit development and ripening of five loquat (Eriohotrya jnponica Lindl.) cultivars. Food Science and Technology International, 9, 43–51.Bantogn A. 2000. Gene expression of NAD+-dependent sorbitol dehvdrogenase and NADP-dependent sorbitol-6 –phosphate dehvdrogenase during development of loquat (Eriobotrya japonica Lindl.) fruit. Journal of the Japanese Society for Horticultural Science, 69, 231–236.Bantog N, Shiratake K, Yamaki S. 1999. Changes in sugar content and sorbitol and sucrose-related enzyme activities during development of loquat (Eriobotrya japonica Lindl. cv. Mogi) fruit. Journal of the Japanese Society for Horticultural Science, 68, 942–948.Carrera L, Sanzol J, Herrero M, Hormaza J I. 2009. Genomic characterization of self-incompatibility ribonucleases (S-RNases) in loquat (Eriobotrya japonica Lindl.) (Rosaceae, Pyrinae). Molecular Breeding, 23, 539–551.Chen J W, Feng J J, Qin Q P, Liu X K, Wu J, Xie M. 2006. Characteristics of sugar metabolism and accumulation in GA3, induced parthcnocarpic white-fleshed loquat “Ninghaibai” fruit. Acta Horticulturac Sinica, 33, 471–476. (in Chinese)Chen J W, Xu H X, Xie M, Wu Y Y, Zhang H Q, Feng J J 2010. Difference in sugar accumulation and metabolism between “Dahongpao” red-fleshed loquat and “Ninghaibai” white-fleshed loquat (Eriobotrya japonica Lindl.). Acta Horticulturae Sinica, 37, 997–1002. (in Chinese)Chen Q Y, Zhou J Y, Zhang B, Fu X M, Song X Q, Li X, Xu C J, Chen K S. 2010. Sugar composition difference between white- and red-fleshed loquat fruits and its relation with activities of sucrose-metabolizing enzymes. Journal of Fruit Science, 27, 616–621. (in Chinese)Fukuda S, Yoshida T, Hiehata N, Sato Y, Terakami S, Yamamoto T, Tominaga Y, Nesumi H. 2009. The inheritance and identification of RAPD marker on fruit flesh color in loquat. Horticultural Research (Japan), 8, 7–11.Fu X M, Kong W B, Peng G, Zhou J Y, Azam M, Xu C J, Grierson D, Chen K S. 2012. Plastid structure and carotenogenic gene expression in red- and white-fleshed loquat (Eriobotrya japonica) fruits. Journal of Experimental Botany, 63, 341–354.Hirat M.1980. Sugar accumulation and development of loquat fruit. Journal of the Japanese Society for Horticultural Science, 49, 347-353.Liang G L, Ren Z C, Yan Y, Huang H, Wu C Q. 1999. Chromosome variation in 8 loquat varieties in Sichuan Province. Acta Horticulturae Sinica, 26, 71–76. (in Chinese)Ma C M, Sun Z, Chen C B, Zhang L L, Zhu S H. 2014. Simultaneous separation and determination of fructose, sorbitol, glucose and sucrose in fruits by HPLC-ELSD. Food Chemistry, 145, 784–788.Nosarzewski M, Archbold D D. 2007. Tissue-specific expression of sorbitol dehydrogenase in apple fruit during early development. Journal of Experimental Botany, 58, 1863–1872.Qin Q P, Lin F F, Zhang L L. 2012. Review of the studies on the molecular and physiological mechanisms of accumulation of sugar and organic acids in loquat fruit. Journal of Zhejiang A&F University, 29, 453–457. (in Chinese)Suzuki Y, Odanaka S, Kanayama Y. 2001. Fructose content and fructose-related enzyme activity during the fruit development of apple and Japanese pear. Journal of the Japanese Society for Horticultural Science, 70, 16–20.Xiong Z M, Zhou C H, Tao J. 2007. Changes in carotenoid content in flesh of different loquat types during fruit coloring. Scientia Agricultura Sinica, 40, 2910–2914. (in Chinese)Xiu L W, Zi Y H, Chun X Y, Xiu Z K, Xiao P S. 2013. Subcellular localization and vacuolar targeting of sorbitol dehydrogenase in apple seed. Plant Science, 210, 36–45.Yamada K, Niwa N, Shiratake, K, Yamaki S. 2001. cDNA cloning of NAD-dependent sorbitol dehvdrogenase from peach fruit and its expression during fruit development. The Journal of Horticultural Science and Biotechnology, 76, 581–587. |
No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|