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Journal of Integrative Agriculture  2018, Vol. 17 Issue (06): 1267-1275    DOI: 10.1016/S2095-3119(17)61846-X
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Molecular mapping of YrTZ2, a stripe rust resistance gene in wild emmer accession TZ-2 and its comparative analyses with Aegilops tauschii
WANG Zhen-zhong1, 2, XIE Jing-zhong2, GUO Li3, ZHANG De-yun3, LI Gen-qiao4, FANG Ti-lin4, CHEN Yong-xing2, LI Jun5, WU Qiu-hong2, LU Ping2, LI Miao-miao3, WU Hai-bin2, 6, ZHANG Huai-zhi2, ZHANG Yan3, YANG Wu-yun5, LUO Ming-cheng7, Fahima Tzion8, LIU Zhi-yong2
1 China Rural Technology Development Center, Beijing 100045, P.R.China
2 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P.R.China
3 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
4 Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK 74078, USA
5 Crop Research Institute, Sichuan Academy of Agriculture Sciences, Chengdu 610066, P.R.China
6 China National Seed Group Co., Ltd., Beijing 100045, P.R.China
7 Department of Plant Sciences, University of California, Davis, CA 95616, USA
8 Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel
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Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating disease that can cause severe yield losses.  Identification and utilization of stripe rust resistance genes are essential for effective breeding against the disease.  Wild emmer accession TZ-2, originally collected from Mount Hermon, Israel, confers near-immunity resistance against several prevailing Pst races in China.  A set of 200 F6:7 recombinant inbred lines (RILs) derived from a cross between susceptible durum wheat cultivar Langdon and TZ-2 was used for stripe rust evaluation.  Genetic analysis indicated that the stripe rust resistance of TZ-2 to Pst race CYR34 was controlled by a single dominant gene, temporarily designated YrTZ2.  Through bulked segregant analysis (BSA) with SSR markers, YrTZ2 was located on chromosome arm 1BS flanked by Xwmc230 and Xgwm413 with genetic distance of 0.8 cM (distal) and 0.3 cM (proximal), respectively.  By applying wheat 90K iSelect SNP genotyping assay, 11 polymorphic loci (consisting of 250 SNP markers) closely linked to YrTZ2 were identified.  YrTZ2 was further delimited into a 0.8-cM genetic interval between SNP marker IWB19368 and SSR marker Xgwm413, and co-segregated with SNP marker IWB28744 (co-segregated with 28 SNP).  Comparative genomics analyses revealed high level of collinearity between the YrTZ2 genomic region and the orthologous region of Aegilops tauschii 1DS.  The genomic region between loci IWB19368 and IWB31649 harboring YrTZ2 is orthologous to a 24.5-Mb genomic region between AT1D0112 and AT1D0150, spanning 15 contigs on chromosome 1DS.  The genetic and comparative maps of YrTZ2 provide a framework for map-based cloning and marker-assisted selection of YrTZ2.
Keywords:  Triticum dicoccoides       Puccinia striiformis f. sp. tritici        SNP        comparative genomics  
Received: 12 June 2017   Accepted: 05 June 2018
Fund: This work was financially supported by the Science and Technology Service Network Initiative of Chinese Academy of Sciences (KFJ-STS-ZDTP-024).
Corresponding Authors:  Correspondence LIU Zhi-yong, Tel: +86-10-64806422,    
About author:  WANG Zhen-zhong, E-mail:;

Cite this article: 

WANG Zhen-zhong, XIE Jing-zhong, GUO Li, ZHANG De-yun, LI Gen-qiao, FANG Ti-lin, CHEN Yongxing, LI Jun, WU Qiu-hong, LU Ping, LI Miao-miao, WU Hai-bin, ZHANG Huai-zhi, ZHANG Yan, YANG Wu-yun, LUO Ming. 2018. Molecular mapping of YrTZ2, a stripe rust resistance gene in wild emmer accession TZ-2 and its comparative analyses with Aegilops tauschii. Journal of Integrative Agriculture, 17(06): 1267-1275.

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