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| Improved drought tolerance by α-naphthaleneacetic acid-induced ROS accumulation in two soybean cultivars |
| XING Xing-hua, FANG Chuan-wen, LI Long, JIANG Hong-qiang, ZHOU Qin, JIANG Hai-dong, WANG Shao-hua |
| Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture/High-Tech Key Laboratory of Information Agriculture of Jiangsu Province, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, P.R.China |
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Abstract Drought is a major abiotic factor limiting agricultural crop production. The objective of this study was to investigate whether α-naphthaleneacetic acid (NAA) confers drought tolerance to soybeans and if such tolerance is correlated with the early reactive oxygen species (ROS) accumulation in leaves. The plants of soybean (Glycine max [L.] Merr.) cv. Nannong 99-6 and cv. Kefeng 1 were foliar treated with 40 mg L−1 NAA at the beginning of bloom and then exposed to water stress for 10 d. We monitored changes in ROS levels, lipid peroxidation and antioxidant system as well as plant biomass during the drought treatment. The results showed that drought stress significantly depressed the growth and yield regardless of spraying NAA. However, drought-stressed plants treated with NAA showed much higher plant biomass and yield than those without NAA. The ROS levels increased in stressed Kefeng 1 but not in stressed Nannong 99-6 2–4 days after the treatment (DAT). During 6–10 DAT, stressed Kefeng 1 had greater increase in the levels of superoxide dismutase (SOD), guaiacol peroxidase (POD), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione peroxidase (GPX), γ-glutamylcysteine synthetase (γ-GCS), reduced ascorbate (AsA), and glutathione (GSH), smaller increase in ROS and malondialdehyde (MDA) as compared with stressed Nannong 99-6. Low ROS prevented ROS from directly reacting with membrane lipid during this stage and, consequently, reduced the cell damage. NAA application elevated ROS levels at 4 DAT, and then increased antioxidant capacity and blocked the increase in the MDA and ROS in stressed Nannong 99-6 and Kefeng 1. Overall, the results indicate that NAA application effectively alleviates the adverse effects of drought stress, which is partially attributable to increase in antioxidant ability and decrease in lipid peroxidation induced by the early ROS accumulation triggered by NAA.
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Received: 14 August 2015
Accepted:
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| Fund: This study was funded by the program supported by the National Key Technologies R&D Program of China during the 11th Five-Year Plan period (2009BADA8B02) and the Fundamental Research Funds for the Central Universities, China (KYZ201202-3). |
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Corresponding Authors:
JIANG Hai-dong, Tel/Fax: +86-25-84395713, E-mail: hdjiang@njau.edu.cn; WANG Shao-hua, Tel: +86-25-84395313, Fax: +86-25-84395845, E-mail: wangsh@njau.edu.cn
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Cite this article:
XING Xing-hua, FANG Chuan-wen, LI Long, JIANG Hong-qiang, ZHOU Qin, JIANG Hai-dong, WANG Shao-hua.
2016.
Improved drought tolerance by α-naphthaleneacetic acid-induced ROS accumulation in two soybean cultivars. Journal of Integrative Agriculture, 15(8): 1770-1784.
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| Bai Y, Feng H, Li Y, Dong D, Li Y, Niu J. 2012. Cloning and expression analysis of γ-ECS and hGSHS genes in soybean under stress of phosphorus deficiency and aluminum toxicity. Southwest China Journal of Agricultural Sciences, 25, 1159–1163. (in Chinese)Battal P, Erez ME, Turker M, Berber I. 2008. Molecular and physiological changes in maize (Zea mays) induced by exogeneous NAA, ABA and MeJA during cold stress. Aannales Botanici Fennici, 45, 173–185.Bradford M M. 1976. A rapid and sensitive method for the quantitation of micro-gram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.Cui W, Chang Z, Li N. 2013. Effect of drought stress on physiology ecology and yield of soybean. Journal of Water Resources and Water Engineering, 24, 20–24. (in Chinese)Elstner E F, Heupel A. 1976. Inhibition of nitrite formation from hydroxylammoniumchloride: A simple assay for superoxide dismutase. Analytical Biochemistry, 70, 616–620.Gadallah M A A. 2000. Effects of indole-3-acetic acid and zinc on the growth, osmotic potential and soluble carbon and nitrogen components of soybean plants growing under water deficit. Journal of Arid Environments, 44, 451–467.Gechev T S, Breusegem F V, Stone J M, Denev I, Laloi C. 2006. Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. Bioessays, 28, 1091–1101.Gechev T S, Gadjev I, Breusegem V F, Inzé D, Dukiandjieva S, Tonevaa V, Minkov I. 2002. Hydrogen peroxide protects tobacco from oxidative stress by inducing a set of antioxidant enzymes. Cellular and Molecular Life Sciences, 59, 708–714.Gill S S, Tuteja N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48, 909–930.Heath R L, Packer L. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125, 189–198.Hsu Y T, Kao C H. 2007. Heat shock-mediated H2O2 accumulation and protection against Cd toxicity in rice seedlings. Plant and Soil, 300, 137–147.IshibashiaY, Yamaguchib H, Yuasab T, Iwaya-Inoueb M, Arimaa S, Zheng S. 2011. Hydrogen peroxide spraying alleviates drought stress in soybean plants. Journal of Plant Physiology, 168, 1562–1567.Ivanchenko M G, den Os D, Monshausen G B, Dubrovsky J G, Bedná?ová A, Krishnan N. 2013. Auxin increases the hydrogen peroxide (H2O2) concentration in tomato (Solanum lycopersicum) root tips while inhibiting root growth. Annals of Botany, 112, 1107–1116.Jiang M, Zhang J. 2002. Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings. Planta, 215, 1022–1030.Joo J H, Bae Y S, Lee J S. 2001. Role of auxin-induced reactive oxygen species in root gravitropism. Plant Physiology, 126, 1055–1061.Jubany-Marí T, Munné-Bosch S, López-Carbonell M, Alegre L. 2009. Hydrogen peroxide is involved in the acclimation of the Mediterranean shrub, Cistus albidus L., to summer drought. Journal of Experimental Botany, 60, 107–120.Kang G, Wang C, Sun G, Wang Z. 2003. Salicylic acid changes activities of H2O2-metabolizing enzymes and increases the chilling tolerance of banana seedlings. Environmental and Experimental Botany, 50, 9–15.Kaya C, Tuna A L, Dikilitas M, Cullu M A. 2010. Responses of some enzymes and key growth parameters of salt-stressed maize plants to foliar and seed applications of kinetin and indole acetic acid. Journal of Plant Nutrition, 33, 405–422.Khanna-Chopra R, Selote D S. 2007. Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than -susceptible wheat cultivar under field conditions. Environmental and Experimental Botany, 60, 276–283.Kholová J, Hash C T, Kumar P L, Yadav R S, Ko?ová M, Vadez V. 2010 Terminal drought-tolerant pearl millet [Pennisetum glaucum (L.) R. Br.] have high leaf ABA and limit transpiration at high vapour pressure deficit. Journal of Experimental Botany, 61, 1431–1440.Lan H, Jia H. 2014. The Effects of NAA on leaf senescence of potted Malus Spectabilis. Modern Landscape Architecture, 11, 34–38. (in Chinese)Law M, Charles S, Halliwell B. 1983. Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts. Biochemical Journal, 210, 899–903.Liang P, Xing X, Zhou Q, Han L, Tian Y, Zhang G, Xing H, Jiang H. 2011. Effect of NAA on growth and photosynthetic characteristic of soybean seedling under drought and re-watering. Soybean Science, 30, 50–55. (in Chinese)Liu Q, Katou K, Okamoto H. 1992. Effects of exogenous auxin on the regulation of elongation growth of excised segments of Vigna hypocotyls under osmotic stress. Plant Cell Physiology, 33, 915–919.Loggini B, Scartazza A, Brugnoli E, Navari-Izzo F. 1999. Antioxidant defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought. Plant Physiology, 119, 1091–1099.Meckel L, Egli D B, Phillips R E, Radcliffe D, Leggett J E. 1984. Effect of moisture stress on seed growth in soybeans. Agronomy Journal, 75, 1027–1031. Moloi M J, Westhuizen A J. 2006. The reactive oxygen species are involved in resistance responses of wheat to the Russian wheat aphid. Journal of Plant Physiology, 163, 1118–1125.Neill S, Desikan R, Hancock G. 2002. Hydrogen peroxide signalling. Current Opinion in Plant Biology, 5, 388–395.Notor G, Foyer C H. 1998. Acrorbate and glutathione: Keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology, 49, 249–279.Pinheiro H A, DaMatta F M, Chaves A R M, Fontes E P B, Loureiro M E. 2004. Drought tolerance in relation to protection against oxidative stress in clones of Coffea canephora subjected to long-term drought. Plant Science, 167, 1307–1314.Schmidt R, Mieulet D, Hubberten H M, Obata T, Hoefgen R, Fernie A R, Fisahn J, San Segundo B, Guiderdoni E, Schippers J H M, Mueller-Roeber B. 2013. Salt-responsive ERF1 regulates reactive oxygen species-dependent signaling during the initial response to salt stress in rice. The Plant Cell, 25, 2115–2131Shan C, Liang Z. 2010. Jasmonic acid regulates ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress. Plant Science, 178, 130–139.Song X, She X, He J, Huang C, Song T. 2006. Cytokinin- and auxin-induced stomatal opening involves a decrease in levels of hydrogen peroxide in guard cells of Vicia faba. Functional Plant Biology, 33, 573–583.Tan W, Liu J, Dai T, Jing Q, Cao W, Jiang D. 2008. Alterations in photosynthesis and antioxidant enzyme activity in winter wheat subjected to post-anthesis water-logging. Photosynthetica, 46, 21–27.Tognetti V B, Mühlenbock P E R, Van Breusegem F. 2012. Stress homeostasis - the redox and auxin perspective. Plant, Cell and Environment, 35, 321–333.De Tullio M C, Jiang K, Feldman L J. 2010. Redox regulation of root apical meristem organization: Connecting root development to its environment. Plant Physiology and Biochemistry, 48, 328–336.Vranová E, Inzé D, Breusegem F V. 2002. Signal transduction during oxidative stress. Journal of Experimental Botany, 53, 1227–1236.Wang C, Yang A, Yin H, Zhang J. 2008. Influence of water stress on endogenous hormone contents and cell damage of maize seedlings. Journal of Integrative Plant Biology, 50, 427–434.Wang S, Liang D, Li C, Hao Y, Ma F, Shu H. 2012. Influence of drought stress on the cellular ultrastructure and antioxidant system in leaves of drought-tolerant and drought-sensitive apple rootstocks. Plant Physiology and Biochemistry, 51, 81–89.Weiler E W, Jordan P S, Conrad W. 1981. Levels of indole-3-acetic acid in intact and decapitated coleoptiles as determined by a specific and highly sensitive solid-phase enzyme immunoassay. Planta, 153, 561–571.Wilkinson S, Davies W J. 2010. Drought, ozone, ABA and ethylene: New insights from cell to plant to community. Plant, Cell & Environment, 33, 510–525.Xu M. 2003. Comparative studies on ascorbic acid biosynthesis and accumulation of different soybean cultivars during sprouting. Journal of the Chinese Cereals and Oils Association, 18, 51–58. (in Chinese)Yan C, Wang W, Tu X, Wang C, Zhang L, Du Q, Song S. 2013. Effect of drought stress at different growth stage on yield and root characteristics of soybean. Soybean Science, 32, 59–63. (in Chinese)Yang J, Zhang J, Wang Z, Zhu Q, Wang W. 2001. Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiology, 127, 315–323.Ye N, Zhu G, Liu Y, Li Y, Zhang J. 2011. ABA controls H2O2 accumulation through the induction of OsCATB in rice leaves under water stress. Plant and Cell Physiology, 52, 689–698.Zhang A, Jiang M, Zhang J, Ding H, Xu S, Hu X, Tan M. 2007. Nitric oxide induced by hydrogen peroxide mediates abscisic acid-induced activation of the mitogen-activated protein kinase cascade involved in antioxidant defense in maize leaves. New Phytologist, 175, 36–50. |
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