|
|
|
Long-term rice-rice-green manure rotation changing the microbial communities in typical red paddy soil in South China |
GAO Song-juan, ZHANG Ren-gang, CAO Wei-dong, FAN Yuan-yuan, GAO Ju-sheng, HUANG Jing, BAI Jin-shun, ZENG Nao-hua, CHANG Dan-na, Shimizu Katsu-yoshi, Kristian Thorup-Kristensen |
1、Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning,
Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2、The Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3、College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R.China
4、Soil and Fertilizer Institute, Qinghai Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, P.R.China
5、College of Resources and Environmental Sciences, China Agricultural University, Beijing 100083, P.R.China
6、Red Soil Experimental Station in Hengyang, Chinese Academy of Agricultural Sciences, Qiyang 426182, P.R.China
7、Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan
8、Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen 2630, Denmark |
|
|
摘要 On the basis of a long-term (30 years) field experiment that involved four rotation systems, rice-rice-winter fallow (RRF), rice-rice-ryegrass (RRG), rice-rice-rape (RRP), and rice-rice-milk vetch (RRV), this study described the effects of green manure on the microbial communities in the red paddy soils using 454 pyrosequencing for the 16S rRNA gene. The Chao1 richness and non-parametric Shannon’s index increased in all soil samples that received green manure treatments. The communities’ structures with the green manure applications were significantly dissimilar from that under the winter fallow. Using Metastats tests, many genera in the RRG, RRP and RRV soils were significantly different from those in the RRF soil, including a number of genera that functioned in the nitrogen and sulfur cycles. Analyses of the genera with these functions revealed the shifts in microbial ecosystem functions after long-term green manuring. Changes in the microbial communities increased the ammonium supply and decreased the soil acidification in green-manure-amended soils. Together, these data suggested powerful effects of green manure on both the microbial communities and the biogeochemical cycle driven by the shifts in bacterial functional groups.
Abstract On the basis of a long-term (30 years) field experiment that involved four rotation systems, rice-rice-winter fallow (RRF), rice-rice-ryegrass (RRG), rice-rice-rape (RRP), and rice-rice-milk vetch (RRV), this study described the effects of green manure on the microbial communities in the red paddy soils using 454 pyrosequencing for the 16S rRNA gene. The Chao1 richness and non-parametric Shannon’s index increased in all soil samples that received green manure treatments. The communities’ structures with the green manure applications were significantly dissimilar from that under the winter fallow. Using Metastats tests, many genera in the RRG, RRP and RRV soils were significantly different from those in the RRF soil, including a number of genera that functioned in the nitrogen and sulfur cycles. Analyses of the genera with these functions revealed the shifts in microbial ecosystem functions after long-term green manuring. Changes in the microbial communities increased the ammonium supply and decreased the soil acidification in green-manure-amended soils. Together, these data suggested powerful effects of green manure on both the microbial communities and the biogeochemical cycle driven by the shifts in bacterial functional groups.
|
Received: 21 August 2015
Accepted:
|
Fund: This study was supported by the Special Fund for Agro-scientific Research in the Public Interest of China (201103005) and the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (2013–2017) |
Corresponding Authors:
CAO Wei-dong, Tel: +86-10-82109622,E-mail: caoweidong@caas.cn
E-mail: caoweidong@caas.cn
|
About author: GAO Song-juan, E-mail: gaosongjuan@caas.cn;* These authors contributed equally to this study. |
Cite this article:
GAO Song-juan, ZHANG Ren-gang, CAO Wei-dong, FAN Yuan-yuan, GAO Ju-sheng, HUANG Jing, BAI Jin-shun, ZENG Nao-hua, CHANG Dan-na, Shimizu Katsu-yoshi, Kristian Thorup-Kristensen.
2015.
Long-term rice-rice-green manure rotation changing the microbial communities in typical red paddy soil in South China. Journal of Integrative Agriculture, 14(12): 2512-2520.
|
Arrigo K R. 2005. Marine microorganisms and global nutrientcycles. Nature, 437, 349-355Bending G D, Turner M K, Rayns F, Marx M C, Wood M.2004. Microbial and biochemical soil quality indicators andtheir potential for differentiating areas under contrastingagricultural management regimes. Soil Biology andBiochemistry, 36, 1785-1792Brochier-Armanet C, Boussau B, Gribaldo S, Forterre P.2008. Mesophilic Crenarchaeota: Proposal for a thirdarchaeal phylum, the Thaumarchaeota. Nature ReviewsMicrobiology, 6, 245-252Cao W D, Huang H X. 2009. Ideas on restoration anddevelopment of green manures in China. Soil and Fertilizer,4, 1-4 (in Chinese)Chao A. 1984. Nonparametric estimation of the number of classes in a population. Scandinavlan Journal of Statistics,11, 265-270Chao A, Chazdon R L, Colwell R K, Shen T J. 2004. A newstatistical approach for assessing similarity of speciescomposition with incidence and abundance data. EcologyLetters, 8, 148-159Chao A, Chazdon R L, Colwell R K, Shen T J. 2006. Abundancebasedsimilarity indices and their estimation when thereare unseen species in samples. Biometrics, 62, 361-371Chao A, Shen T J. 2003. Nonparametric estimation of Shannon’sindex of diversity when there are unseen species in sample.Environmental and Ecological Statistics, 10, 429-443Chen X P, Zhu Y G, Xia Y, Shen J P, He J Z. 2008. Ammoniaoxidizingarchaea: Important players in paddy rhizospheresoil? Environmental Microbiology, 10, 1978-1987Christensen B, Laake M, Lien T. 1996. Treatment of acid minewater by sulfate-reducing bacteria; results from a benchscale experiment. Water Research, 30, 1617-1624Claesson M J, O’Sullivan O, Wang Q, Nikkila J, Marchesi JR, Smidt H, de Vos W M, Ross R P, O’Toole P W. 2009.Comparative analysis of pyrosequencing and a phylogeneticmicroarray for exploring microbial community structures inthe human distal intestine. PLoS ONE, 4, e6669.Di H J, Cameron K C, Shen J P, Winefield C S, O’CallaghanM, Bowatte S, He J Z. 2009. Nitrification driven by bacteriaand not archaea in nitrogen-rich grassland soils. NatureGeoscience, 2, 621-624Drinkwater L E, Wagoner P, Sarrantonio M. 1998. Legumebasedcropping systems have reduced carbon and nitrogenlosses. Nature, 396, 262-265Edgar R C, Haas B J, Clemente J C, Quince C, Knight R.2011. UCHIME improves sensitivity and speed of chimeradetection. Bioinformatics, 27, 2194-2200Elfstrand S, Båth B, Mårtensson A. 2007a. Influence of variousforms of green manure amendment on soil microbialcommunity composition, enzyme activity and nutrient levelsin leek. Applied Soil Ecology, 36, 70-82Elfstrand S, Hedlund K, Mårtensson A. 2007b. Soil enzymeactivities, microbial community composition and functionafter 47 years of continuous green manuring. Applied SoilEcology, 35, 610-621Gao J S, Cao W D, Li D C, Xu M G, Zeng X B, Nie J, Zhang WJ. 2011. Effects of long-term double-rice and green manurerotation on rice yield and soil organic matter in paddy field.Acta Ecologica Sinica, 31, 4542-4548 (in Chinese)Gao J S, Xu M G, Dong C H, Huang J, Cao W D, Zeng X B,Wen S L, Nie J. 2013. Effects of long-term rice-rice-greenmanure cropping rotation on rice yield and soil fertility. ActaAgronomica Sinica, 39, 343-349 (in Chinese)Good I J. 1953. The population frequencies of species andthe estimation of population parameters. Biometrika, 40,237-264Gribaldo S, Brochier-Armanet C. 2006. The origin and evolutionof Archaea: A state of the art. Philosophical Transactions ofthe Royal Society of London (Series B, Biological Sciences),361, 1007-1022Guo J H, Liu X J, Zhang Y, Shen J L, Han W X, Zhang WF, Christie P, Goulding K W, Vitousek P M, Zhang F S.2010. Significant acidification in major Chinese croplands.Science, 327, 1008-1010Hayden E C. 2013. Gene sequencing leaves the laboratory.Nature, 494, 290-291Hirsch P R. 1996. Population dynamics of indigenous andgenetically modified rhizobia in the field. New Phytologist,133, 159-171Huse S M, Welch D M, Morrison H G, Sogin M L. 2010. Ironingout the wrinkles in the rare biosphere through improved OTUclustering. Environmental Microbiology, 12, 1889-1898Iguchi H, Yurimoto H, Sakai Y. 2011. Methylovulum miyakonensegen. nov., sp. nov., a type I methanotroph isolatedfrom forest soil. International Journal of Systematic andEvolutionary Microbiology, 61, 810-815Ishiyama K, Inoue E, Tabuchi M, Yamaya T, Takahashi H. 2004.Biochemical background and compartmentalized functionsof cytosolic glutamine synthetase for active ammoniumassimilation in rice roots. Plant and Cell Physiology, 45,1640-1647Jetten M S, Niftrik L, Strous M, Kartal B, Keltjens J T, Op denCamp H J. 2009. Biochemistry and molecular biology ofanammox bacteria. Critical Reviews in Biochemistry andMolecular Biology, 44, 65-84Komarnisky L A, Christopherson R J, Basu T K. 2003. Sulfur:Its clinical and toxicologic aspects. Nutrition, 19, 54-61Larssen T, Carmichael G R. 2000. Acid rain and acidificationin China: The importance of base cation deposition.Environmental Pollution, 110, 89-102Leininger S, Urich T, Schloter M, Schwark L, Qi J, Nicol GW, Prosser J I, Schuster S C, Schleper C. 2006. Archaeapredominate among ammonia-oxidizing prokaryotes in soils.Nature, 442, 806-809Lozupone C, Knight R. 2005. UniFrac: A new phylogeneticmethod for comparing microbial communities. Applied andEnvironmental Microbiology, 71, 8228-8235Lozupone C, Lladser M E, Knights D, Stombaugh J, Knight R.2011. UniFrac: An effective distance metric for microbialcommunity comparison. ISME Journal, 5, 169-172Macguidwin A E, Knuteson D L, Connell T, Bland W L, BarteltK D. 2012. Manipulating inoculum densities of Verticilliumdahliae and Pratylenchus penetrans with green manureamendments and solarization influence potato yield.Phytopathology, 102, 519-527MacRae R J, Mehuys G R. 1985. The effect of green manuringon the physical properties of temperate-area soils.Advances in Soil Science, 3, 71-94Maier R M. 2000. Biogeochemical cycling. EnvironmentalMicrobiology, 2, 428-464Murugan R, Kumar S. 2013. Influence of long-term fertilisationand crop rotation on changes in fungal and bacterialresidues in a tropical rice-field soil. Biology and Fertility ofSoils, 49, 847-856Needleman S B, Wunsch C D. 1970. A general methodapplicable to the search for similarities in the amino acid sequence of two proteins. Journal of Molecular Biology,48, 443-453Pruesse E, Quast C, Knittel K, Fuchs B M, Ludwig W, PepliesJ, Glockner F O. 2007. SILVA: A comprehensive onlineresource for quality checked and aligned ribosomal RNAsequence data compatible with ARB. Nucleic AcidsResearch, 35, 7188-7196Schloss P D. 2008. Evaluating different approaches that testwhether microbial communities have the same structure.ISME Journal, 2, 265-275Schloss P D. 2009. A high-throughput DNA sequence alignerfor microbial ecology studies. PLoS ONE, 4, e8230.Schloss P D, Westcott S L, Ryabin T, Hall J R, Hartmann M,Hollister E B, Lesniewski R A, Oakley B B, Parks D H,Robinson C J, Sahl J W, Stres B, Thallinger G G, Van HornD J, Weber C F. 2009. Introducing mothur: Open-source,platform-independent, community-supported software fordescribing and comparing microbial communities. Appliedand Environmental Microbiology, 75, 7537-7541Schloss P D, Gevers D, Westcott S L. 2011. Reducing theeffects of PCR amplification and sequencing artifacts on16S rRNA-based studies. PLoS ONE, 6, e27310.Schloss P D, Westcott S L. 2011. Assessing and improvingmethods used in operational taxonomic unit-basedapproaches for 16S rRNA gene sequence analysis. Appliedand Environmental Microbiology, 77, 3219-3226Schmid M C, Maas B, Dapena A, van de Pas-Schoonen K, vande Vossenberg J, Kartal B, van Niftrik L, Schmidt I, CirpusI, Kuenen J G, Wagner M, Sinninghe Damste J S, KuypersM, Revsbech N P, Mendez R, Jetten M S, Strous M. 2005.Biomarkers for in situ detection of anaerobic ammoniumoxidizing(anammox) bacteria. Applied and EnvironmentalMicrobiology, 71, 1677-1684Tamaki H, Wright C L, Li X, Lin Q, Hwang C, Wang S,Thimmapuram J, Kamagata Y, Liu W T. 2011. Analysis of16S rRNA amplicon sequencing options on the roche/454next-generation titanium sequencing platform. PLoS ONE,6, e25263.Tejada M, Gonzalez J L, Garcia-Martinez A M, Parrado J.2008a. Effects of different green manures on soil biologicalproperties and maize yield. Bioresource Technology, 99,1758-1767Tejada M, Gonzalez J L, Garcia-Martinez A M, Parrado J.2008b. Application of a green manure and green manurecomposted with beet vinasse on soil restoration: Effects onsoil properties. Bioresource Technology, 99, 4949-4957Wang Q, Garrity G M, Tiedje J M, Cole J R. 2007. NaïveBayesian classifier for rapid assignment of rRNA sequencesinto the new bacterial taxonomy. Applied and EnvironmentalMicrobiology, 73, 5261-5267Wang Y, Qian P Y. 2009. Conservative fragments in bacterial16S rRNA genes and primer design for 16S ribosomal DNAamplicons in metagenomic studies. PLoS ONE, 4, e7401.White J R, Nagarajan N, Pop M. 2009. Statistical methodsfor detecting differentially abundant features in clinicalmetagenomic samples. Plos Computational Biology, 5,e1000352.Yang L, Cao W D, Bai J S, Xiong J, Gao S J. 2013. Effect ofcombined application of February Orchid (Orychophragmusviolaceus L.) and chemical fertilizer on nutrient uptake andutilization of spring maize. Journal of Plant Nutrition andFertilizer, 19, 799-807 (in Chinese)Yang Z P, Gao J S, Zheng S X, Nie J, Xu M G, Xie J, Liao Y L.2011. Effects of long-term winter planting-green manure onmicrobial properties and enzyme activities in reddish paddysoil. Soils, 43, 576-583 (in Chinese)Ye X, Liu H, Li Z, Wang Y, Wang Y, Wang H, Liu G. 2014.Effects of green manure continuous application on soilmicrobial biomass and enzyme activity. Journal of PlantNutrition, 37, 498-508Zhang L M, Hu H W, Shen J P, He J Z. 2012. Ammonia-oxidizingarchaea have more important role than ammonia-oxidizingbacteria in ammonia oxidation of strongly acidic soils. ISMEJournal, 6, 1032-1045Zheng Y, Zhang L M, Zheng Y M, Di H, He J Z. 2008. Abundanceand community composition of methanotrophs in a Chinesepaddy soil under long-term fertilization practices. Journal ofSoils and Sediments, 8, 406–414. |
No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|