green manure , red paddy soil , dissolved organic matter , ultraviolet-visible spectra , Fourier transform infrared spectra," /> green manure , red paddy soil , dissolved organic matter , ultraviolet-visible spectra , Fourier transform infrared spectra,"/> green manure , red paddy soil , dissolved organic matter , ultraviolet-visible spectra , Fourier transform infrared spectra,"/>
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Journal of Integrative Agriculture  2018, Vol. 17 Issue (08): 1852-1860    DOI: 10.1016/S2095-3119(17)61901-4
Agro-ecosystem & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Effects of long-term green manure application on the content and structure of dissolved organic matter in red paddy soil
GAO Song-juan1, 2*, GAO Ju-sheng2, 3*, CAO Wei-dong2, 4, ZOU Chun-qin1, HUANG Jing2, 3, BAI Jin-shun2, DOU Fu-gen5 
1 College of Resources and Environmental Sciences, Center for Resources, Environment and Food Security/Key Laboratory of Plant-Soil Interactions, Ministry of Education, China Agricultural University, Beijing 100193, P.R.China
2 Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3 Red Soil Experimental Station in Hengyang, Chinese Academy of Agricultural Sciences, Qiyang 426182, P.R.China
4 Soil and Fertilizer Institute, Qinghai Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, P.R.China
5 Texas A&M AgriLife Research Center at Beaumont, Texas 77713, USA
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摘要  Received  27 October, 2017    Accepted  11 January, 2018

Dissolved organic matter (DOM) plays important roles in soil biogeochemistry activity and nutrients transportation in soils, but studies regarding the long-term effects of green manures on the content and structure of DOM in red paddy soil have not been reported yet.  A long-term green manure experiment established in 1982 was utilized to test the DOM contents in different treatments, and the spectral characteristics of DOM were investigated by using ultraviolet-visible (UV-Vis) spectrometry and Fourier transform infrared (FTIR) spectrometry.  The experiment included four cropping systems: rice-rice-milk vetch (RRV), rice-rice-rape (RRP), rice-rice-ryegrass (RRG) and rice-rice-winter fallow (RRF), among them, milk vetch, rape, and ryegrass are popular winter green manure species in southern China.  The results showed that the content of dissolved organic carbon (DOC), which is widely used to estimate the concentration of DOM, was significantly promoted after the incorporation of green manures compared with the other sampling stages.  The contents of aromatic groups and the degree of humification of DOM increased in RRV and RRP, suggesting more complex compositions of the soil DOM after long-term application of milk vetch and rape.  The contents of phenol, alcohol and carboxylic acid group at the mature stage of early rice were significantly higher than those at the stage of after green manures turned over, especially for the RRV treatment.  The absorption ratio of FTIR indicated that winter plantation of rape increased the aromatic-C/aliphatic-C ratio, and ryegrass increased the aromatic-C/carboxyl-C ratio.  In conclusion, long-term planting of milk vetch and rape as green manures increased the degree of aromaticity, humification and average molecular weight of DOM, and made the DOM more stable in red paddy soil. 
Keywords:  green manure ')" href="#">  
Received: 27 October 2017   Accepted:
Fund: This study was supported by the earmarked fund for China Agriculture Research System (2013–2017) and the Chinese Outstanding Talents Program in Agricultural Sciences.
Corresponding Authors:  Correspondence ZOU Chun-qin, Tel: +86-10-62733539, E-mail:; CAO Wei-dong, Tel: +86-10-82109622, E-mail:    
About author:  GAO Song-juan, E-mail:; * These authors contributed equally to this study.
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GAO Song-juan
GAO Ju-sheng
CAO Wei-dong
ZOU Chun-qin
BAI Jin-shun
DOU Fu-gen

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GAO Song-juan, GAO Ju-sheng, CAO Wei-dong, ZOU Chun-qin, HUANG Jing, BAI Jin-shun, DOU Fu-gen. 2018. Effects of long-term green manure application on the content and structure of dissolved organic matter in red paddy soil. Journal of Integrative Agriculture, 17(08): 1852-1860.

Blodau C, Bauer M, Regenspurg S, Macalady D. 2009. Electron accepting capacity of dissolved organic matter as determined by reaction with metallic zinc. Chemical Geology, 260, 186–195.
Chang D N, Cao W D, Bai J S, Gao S J, Wang X C, Zeng N H, Shimizu K. 2017. Effects of green manures on soil dissolved organic matter in moisture soil in North China. Spectroscopy and Spectral Analysis, 27, 221–226. (in Chinese)
Chantigny M H. 2003. Dissolved and water-extractable organic matter in soils: A review on the influence of land use and management practices. Geoderma, 113, 357–380.
Chen W, Westerhoff P, Leenheer J A, Booksh K. 2003. Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter. Environmental Science & Technology, 37, 5701–5710.
Chin Y P, Alken G R, Loughlin E O. 1994. Molecular weight, polydispersity, and spectroscopic properties of aquatic humic substances. Environmental Science & Technology, 28, 1853–1858.
Cook B D, Allan D L. 1992. Dissolved organic carbon in old field soils: Compositional changes during the biodegradation of soil organic matter. Soil Biology & Biochemistry, 24, 595–600.
Don A, Kalbitz K. 2005. Amounts and degradability of dissolved organic carbon from doliar litter at different decomposition stages. Soil Biology & Biochemistry, 37, 2171–2179.
Dorado J, González-Vila F J, Zancada M C, Almendros G, López-Fando C. 2003. Pyrolytic descriptors responsive to changes in humic acid characteristics after long-term sustainable management of dryland farming systems in Central Spain. Journal of Analytical and Applied Pyrolysis, 68, 299–314.
Elfstrand S, Hedlund K, Mårtensson A. 2007. Soil enzyme activities, microbial community composition and function after 47 years of continuous green manuring. Applied Soil Ecology, 35, 610–621.
Fang H J, Cheng S L, Yu G R, Xu M J, Wang Y S, Li L S, Dang X S, Wang L, Li Y N. 2014. Experimental nitrogen deposition alters the quantity and quality of soil dissolved organic carbon in an alpine meadow on the Qinghai-Tibetan Plateau. Applied Soil Ecology, 81, 1–11.
FAO (Food and Agriculture Organization). 2006. World Reference Base for Soil Resources 2006. World Soil Resources Report. p. 103.
Fibor T, Rékási M. 2011. Factors controlling dissolved organic carbon (DOC), dissolved organic nitrogen (DON) and DOC/DON ratio in arable soils based on a dataset from Hungary. Geoderma, 162, 312–318.
Fu Q L, He J Z, Blaney L, Zhou D M. 2016. Roxarsone binding to soil-derived dissolved organic matter: Insights from multi-spectroscopic techniques. Chemosphere, 155, 225–233.
Gao J S, Xu M G, Dong C H, Huang J, Cao W D, Zeng X B, Wen S L, Nie J. 2013. Effects of long-term rice-rice-green manure cropping rotation on rice yield and soil fertility. Acta Agronomica Sinica, 39, 34–39. (in Chinese)
Guggenberger G, Kaiser K. 2003. Dissolved organic matter in soil: Challenging the paradigm of sorptive preservation. Geoderma, 113, 293–310.
Guppy C N, Menzies N W, Moody P W, Blamey F P C. 2005. Competitive sorption reactions between phosphorus and organic matter in soil: a review. Australian Journal of Soil Research, 43, 189–204.
He X, Xi B, Wei Z, Guo X, Li M, An D, Liu H. 2011. Spectroscopic characterization of water extractable organic matter during composting of municipal solid waste. Chemosphere, 82, 541–548.
He X S, Xi B D, Jiang Y H, Li M X, Yu H B, An D, Yang Y, Liu H L. 2012. Elemental and spectroscopic methods with chemometric analysis for characterizing composition and transformation of dissolved organic matter during chicken manure composting. Environmental Technology, 33, 2033–2039.
Hiriart-Baer V P, Diep N, Smith R E H. 2008. Dissolved organic matter in the great lakes: Role and nature of allochthonous material. Journal of Great Lakes Research, 34, 383–394.
Hunt J F, Ohno T. 2007. Characterization of fresh and decomposed dissolved organic matter using excitation-emission matrix fluorescence spectroscopy and multiway analysis. Journal of Agricultural and Food Chemistry, 55, 2121–2128.
Hur J, Williams M A, Schlautman M A. 2006. Evaluating spectroscopic and chromatographic techniques to resolve dissolved organic matter via end member mixing analysis. Chemosphere, 63, 387–402.
Ilina S M, Drozdova O Y, Lapitskiy S A, Alekhin Y V, Demin V V, Zavgorodnyaya Y A, Shirokova L S, Viers J, Pokrovsky O S. 2014. Size fractionation and optical properties of dissolved organic matter in the continuum soil solution-bog-river and terminal lake of a boreal watershed. Organic Geochemistry, 66, 14–24.
Jaffé R, Boyer J N, Lu X, Maie N, Yang C, Scully N M, Mock S. 2004. Source characterization of dissolved organic matter in a subtropical mangrove-dominated estuary by fluorescence analysis. Marine Chemistry, 84, 195–210.
Jones D L, Simfukwe P, Hill P W, Mills R T E, Emmett B A. 2014. Evaluation of dissolved organic carbon as a soil quality indicator in national monitoring schemes. PLoS ONE, 9, e90882.
Kalbitz K, Schmerwitz J, Schwesig D, Matzner E. 2003a. Biodegradation of soil-derived dissolved organic matter as related to its properties. Geoderma, 113, 273–291.
Kalbitz K, Schwesig D, Rethemeyer J, Matzner E. 2005. Stabilization of dissolved organic matter by sorption to the mineral soil. Soil Biology & Biochemistry, 37, 1319–1331.
Kalbitz K, Schwesig D, Schmerwitz J, Kaiser K, Haumaier L, Glaser B, Ellerbrock R, Leinweber P. 2003b. Changes in properties of soil-derived dissolved organic matter induced by biodegradation. Soil Biology & Biochemistry, 35, 1129–1142.
Kalbitz K, Sonlinger S, Park J H, Michalzik B, Matzner E. 2000. Controls on the dynamics of dissolved organic matter in soils: A review. Soil Science, 165, 277–304.
Kuzyakov Y. 2010. Priming effects: Interactions between living and dead organic matter. Soil Biology & Biochemistry, 42, 1363–1371.
Lguirati A, Ait Baddi G, El Mousadik A, Gilard V, Revel J C, Hafidi M. 2005. Analysis of humic acids from aerated and non-aerated urban landfill composts. International Biodeterioration & Biodegradation, 56, 8–16.
Liu Y, Li F B, Xia W, Xu J M, Yu X S. 2013. Association between ferrous iron accumulation and pentachlorophenol degradation at the paddy soil-water interface in the presence of exogenous low-molecular-weight dissolved organic carbon. Chemosphere, 91, 1547–1555.
Long G Q, Jiang Y J, Sun B. 2015. Seasonal and inter-annual variation of leaching of dissolved organic carbon and nitrogen under long-term manure application in an acidic clay soil in subtropical China. Soil & Tillage Research, 146, 270–278.
Lu R K. 2000. Analytical Method for Soil and Agro-Chemical. Agricultural Science and Technology Press, China. (in Chinese)
Madari B E, Reeves J B, Machado P L O A, Guimarães C M, Torres E, McCarty G W. 2006. Mid- and near-infrared spectroscopic assessment of soil compositional parameters and structural indices in two Ferralsols. Geoderma, 136, 245–259.
Marschner B, Kalbitz K. 2003. Controls of bioavailability and biodegradability of dissolved organic matter in soils. Geoderma, 113, 211–235.
Qiu Q Y, Wu L F, Ouyang Z, Li B B, Xu Y Y, Wu S S, Gregorich E G. 2015. Effects of plant-derived dissolved organic matter (DOM) on soil CO2 and N2O emissions and soil carbon and nitrogen sequestrations. Applied Soil Ecology, 96, 122–130.
Shang F Z, Ren S M, Yang P L, Li C S, Ma N. 2015. Effects of different fertilizer and irrigation water types, and dissolved organic matter on soil C and N mineralization in crop rotation farmland. Water Air and Soil Pollution, 226, 395–420.
Smebye A, Alling V, Vogt R D, Gadmar T C, Mulder J, Cornelissen G, Hale S E. 2016. Biochar amendment to soil changes dissolved organic matter content and composition. Chemosphere, 142, 100–105.
Smidt E, Eckhardt K U, Lechner P, Schulten H R, Leinweber P. 2005. Characterization of different decomposition stages of biowaste using FTIR spectroscopy and pyrolysis-field ionization mass spectrometry. Biogeochemistry, 16, 67–79.
Swietlik J, Dabrowska A, Raczyk-Stanislawiak U, Nawrocki J. 2004. Reactivity of natural organic matter fractions with chlorine dioxide and ozone. Water Research, 38, 547–558.
Uyguner C S, Bekbolet M. 2005. Implementation of spectroscopic parameters for practical monitoring of natural organic matter. Desalination, 176, 47–55.
Uyguner-Demirel C S, Bekbolet M. 2011. Significance of analytical parameters for the understanding of natural organic matter in relation to photocatalytic oxidation. Chemosphere, 84, 1009–1031.
Wershaw R L. 2004. Evaluation of conceptual models of natural organic matter (Humus) from a consideration of the chemical and biochemical processes of humification. Scientific Investigations Report, 5121, 1–49.
Zhang A F, Zhou X, Li M, Wu H M. 2017. Impacts of biochar addition on soil dissolved organic matter characteristics in a wheat-maize rotation system in Loess Plateau of China. Chemosphere, 186, 986–993.
Zhu Z K, Tao L, Li F B. 2013. Effects of dissolved organic matter on adsorbed Fe(II) reactivity for the reduction of 2-nitrophenol in TiO2 suspensions. Chemosphere, 93, 29–34.
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