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| Winter cover crops alter methanotrophs community structure in a double-rice paddy soil |
| LIU Jing-na, ZHU Bo, YI Li-xia, DAI Hong-cui, XU He-shui, ZHANG Kai, HU Yue-gao, ZENG Zhao-hai |
1、College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
2、School of Agronomy, Yangtze University, Jingzhou 434025, P.R.China |
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摘要 Methanotrophs play a vital role in the mitigation of methane emission from soils. However, the influences of cover crops incorporation on paddy soil methanotrophic community structure have not been fully understood. In this study, the impacts of two winter cover crops (Chinese milk vetch (Astragalus sinicus L.) and ryegrass (Lolium multiflorum Lam.), representing leguminous and non-leguminous cover crops, respectively) on community structure and abundance of methanotrophs were evaluated by using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and real-time PCR technology in a double-rice cropping system from South China. Four treatments were established in a completely randomized block design: 1) double-rice cropping without nitrogen fertilizer application, CK; 2) double-rice cropping with chemical nitrogen fertilizer application (200 kg ha–1 urea for entire double-rice season), CF; 3) Chinese milk vetch cropping followed by double-rice cultivation with Chinese milk vetch incorporation, MV; 4) ryegrass cropping followed by double-rice cultivation with ryegrass incorporation, RG. Results showed that cultivating Chinese milk vetch and ryegrass in fallow season decreased soil bulk density and increased rice yield in different extents by comparison with CK. Additionally, methanotrophic bacterial abundance and community structure changed significantly with rice growth. Methanotrophic bacterial pmoA gene copies in four treatments were higher during late-rice season (3.18×107 to 10.28×107 copies g–1 dry soil) by comparison with early-rice season (2.1×107 to 9.62×107 copies g–1 dry soil). Type I methanotrophs absolutely predominated during early-rice season. However, the advantage of type I methanotrophs kept narrowing during entire double-rice season and both types I and II methanotrophs dominated at later stage of late-rice.
Abstract Methanotrophs play a vital role in the mitigation of methane emission from soils. However, the influences of cover crops incorporation on paddy soil methanotrophic community structure have not been fully understood. In this study, the impacts of two winter cover crops (Chinese milk vetch (Astragalus sinicus L.) and ryegrass (Lolium multiflorum Lam.), representing leguminous and non-leguminous cover crops, respectively) on community structure and abundance of methanotrophs were evaluated by using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and real-time PCR technology in a double-rice cropping system from South China. Four treatments were established in a completely randomized block design: 1) double-rice cropping without nitrogen fertilizer application, CK; 2) double-rice cropping with chemical nitrogen fertilizer application (200 kg ha–1 urea for entire double-rice season), CF; 3) Chinese milk vetch cropping followed by double-rice cultivation with Chinese milk vetch incorporation, MV; 4) ryegrass cropping followed by double-rice cultivation with ryegrass incorporation, RG. Results showed that cultivating Chinese milk vetch and ryegrass in fallow season decreased soil bulk density and increased rice yield in different extents by comparison with CK. Additionally, methanotrophic bacterial abundance and community structure changed significantly with rice growth. Methanotrophic bacterial pmoA gene copies in four treatments were higher during late-rice season (3.18×107 to 10.28×107 copies g–1 dry soil) by comparison with early-rice season (2.1×107 to 9.62×107 copies g–1 dry soil). Type I methanotrophs absolutely predominated during early-rice season. However, the advantage of type I methanotrophs kept narrowing during entire double-rice season and both types I and II methanotrophs dominated at later stage of late-rice.
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Received: 29 April 2015
Accepted:
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| Fund: This study was supported by the National Natural Science Foundation of China (31171509 and 30671222), the Special Fund for Agro-scientific Research in the Public Interest, China (201103001), and the National Key Technology R&D Program for the 12th Five-Year Plan period (2011BAD16B15). |
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Corresponding Authors:
ZENG Zhao-hai,Tel: +86-10-62733847, Mobile: +86-13911686202, Fax: +86-10-62732441, E-mail: zengzhaohai@cau.edu.cn
E-mail: zengzhaohai@cau.edu.cn
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| About author: LIU Jing-na, E-mail: liujingna199066@126.com; ZHU Bo,E-mail: 1984zhubo@163.com;* These authors contributed equally to this study |
Cite this article:
LIU Jing-na, ZHU Bo, YI Li-xia, DAI Hong-cui, XU He-shui, ZHANG Kai, HU Yue-gao, ZENG Zhao-hai.
2016.
Winter cover crops alter methanotrophs community structure in a double-rice paddy soil. Journal of Integrative Agriculture, 15(3): 553-565.
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| Azam F, Lodhi A, Ashraf M. 1991. Availability of soil and fertilizernitrogen to wetland rice following wheat straw amendment.Biology and Fertility of Soils, 11, 97-100Bender M, Conrad R. 1995. Effect of CH4 concentrations andsoil conditions on the induction of CH4 oxidation activity.Soil Biology and Biochemistry, 27, 1517-1527Cahyani V R, Matsuya K, Asakawa S, Kimura M. 2003.Succession and phylogenetic composition of bacterialcommunities responsible for the composting process of ricestraw estimated by PCR-DGGE analysis. Soil Science andPlant Nutrition, 49, 619-630Cho Y S, Mineta T, Hidaka K. 2003. Nitrogen fixation andutilization for green manure of common wild legumenarrowleaf vetch (Vicia angustifolia L.). Japan AgriculturalResearch Quarterly, 37, 45-51Conrad R. 2007. Microbial ecology of methanogens andmethanotrophs. Advances in Agronomy, 96, 1-63Costello A M, Lidstrom M E. 1999. Molecular characterizationof functional and phylogenetic genes from naturalpopulations of methanotrophs in lake sediments. Appliedand Environmental Microbiology, 65, 5066-5074Flessa H, Beese F. 1995. Effects of sugarbeet residues on soilredox potential and nitrous oxide emission. Soil ScienceSociety of America Journal, 59, 1044-1051Groot T T, Van Bodegom P M, Harren F J M, Meijer H A J. 2003.Quantification of methane oxidation in the rice rhizosphereusing 13C-labelled methane. Biogeochemistry, 64, 355-372Gutierrez J, Atulba S L, Kim G, Kim P J. 2014. Importance ofrice root oxidation potential as a regulator of CH4 productionunder waterlogged conditions. Biology and Fertility of Soils,50, 861-868Han C, Zhong W H, Shen W S, Cai Z C, Liu B. 2013. TransgenicBt rice has adverse impacts on CH4 flux and rhizosphericmethanogenic archaeal and methanotrophic bacterialcommunities. Plant and Soil, 369, 297-316Henckel T, Roslev P, Conrad R. 2000. Effects of O2 and CH4on presence and activity of the indigenous methanotrophiccommunity in rice field soil. Environmental Microbiology,2, 666-679Holmes A J, Costello A, Lidstrom M E, Murrell J C. 1995.Evidence that participate methane monooxygenase andammonia monooxygenase may be evolutionarily related.FEMS Microbiology Letters, 132, 203-208Horz H P, Yimga M T, Liesack W. 2001. Detection ofmethanotroph diversity on roots of submerged rice plantsby molecular retrieval of pmoA, mmoX, mxaF, and 16SrRNA and Ribosomal DNA, including pmoA-based terminalrestriction fragment length polymorphism profiling. Appliedand Environmental Microbiology, 67, 4177-4185Ikenaga M, Asakawa S, Muraoka Y, Kimura M. 2003. Bacterialcommunities associated with nodal roots of rice plantsalong with the growth stages: Estimation by PCR-DGGEand sequence anals. Soil Science and Plant Nutrition, 49,591-602IPCC (Intergovernmental Panel on Climatic Change). 2007.Climate Change 2007: The Physical Science Basis.Cambridge University Press, Cambridge, United, Kingdomand New York, USA. p. 104.Kim S Y, Gutierrez J, Kim P J. 2012. Considering winter covercrop selection as green manure to control methane emissionduring rice cultivation in paddy soil. Agriculture, Ecosystems& Environment, 161, 130-136Kim S Y, Lee C H, Gutierrez J, Kim P J. 2013. Contribution ofwinter cover crop amendments on global warming potentialin rice paddy soil during cultivation. Plant and Soil, 366,273-286Kirchmann H, Witter E. 1992. Composition of fresh, aerobicand anaerobic farm animal dungs. Bioresource Technology,40, 137-142Kruger M, Frenzel P, Kemnitz D, Conrad R. 2005. Activity,structure and dynamics of the methanogenic archaealcommunity in a flooded Italian rice field. FEMS MicrobiologyEcology, 51, 323-331Lee C H, Do Park K, Jung K Y, Ali M A, Lee D, Gutierrez J,Kim P J. 2010. Effect of Chinese milk vetch (Astragalussinicus L.) as a green manure on rice productivity andmethane emission in paddy soil. Agriculture, Ecosystems& Environment, 138, 343-347Lee H J, Kim S Y, Kim P J, Madsen E L, Jeon C O. 2014.Methane emission and dynamics of methanotrophicand methanogenic communities in a flooded rice fieldecosystem. FEMS Microbiology Ecology, 88, 195-212Liu Y, Lou J, Li F B, Xu J M, Yu X S, Zhu L A, Wang F. 2014.Evaluating oxidation-reduction properties of dissolvedorganic matter from Chinese milk vetch (Astragalus sinicusL.): A comprehensive multi-parametric study. EnvironmentalTechnology, 35, 1916-1927Ma K, Lu Y. 2011. Regulation of microbial methane productionand oxidation by intermittent drainage in rice field soil. FEMSMicrobiology Ecology, 75, 446-456Murase J, Shimizu M, Hayashi M, Matsuya K, Kimura M. 2005.Vertical changes in bacterial communities in percolatingwater of a Japanese paddy field as revealed by PCR-DGGE.Soil Science and Plant Nutrition, 51, 83-90Olson K R, Ebelhar S A, Lang J M. 2010. Cover crop effects oncrop yields and soil organic carbon content. Soil Science,175, 89-98Qiu Q, Noll M, Abraham W R, Lu Y, Conrad R. 2008. Applyingstable isotope probing of phospholipid fatty acids and rRNA in a Chinese rice field to study activity and composition ofthe methanotrophic bacterial communities in situ. The ISMEJournal, 2, 602-614Rao D N, Mikkelsen D S. 1976. Effect of rice straw incorporationon rice plant growth and nutrition. Agronomy Journal, 68,752-756Talgre L, Lauringson E, Roostalu H, Astover A, Makke A. 2012.Green manure as a nutrient source for succeeding crops.Plant Soil and Environment, 58, 275-281Verma T S, Bhagat R M. 1992. Impact of rice straw managementpractices on yield, nitrogen uptake and soil properties in awheat-rice rotation in northern India. Fertilizer Research,33, 97-106Wu L, Ma K, Lu Y. 2009. Rice roots select for type Imethanotrophs in rice field soil. Systematic and AppliedMicrobiology, 32, 421-428Zhang X, Kong J Y, Xia F F, Su Y, He R. 2014. Effects ofammonium on the activity and community of methanotrophsin landfill biocover soils. Systematic and AppliedMicrobiology, 37, 296-304Zhu B, Yi L X, Hu Y G, Zeng Z H, Lin C, Tang H M, Xiao XP. 2014. Nitrogen release from incorporated 15N-labelledChinese milk vetch (Astragalus sinicus L.) residue and itsdynamics in a double rice cropping system. Plant and Soil,374, 331-344Zhu B, Yi L X, Hu Y G, Zeng Z H, Tang H M, Yang G L, Xiao XP. 2012. Effects of Chinese milk vetch (Astragalus sinicusL.) residue incorporation on CH4 and N2O emission from adouble-rice paddy soil. Journal of Integrative Agriculture,11, 1537-1544 |
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