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Journal of Integrative Agriculture  2023, Vol. 22 Issue (4): 1199-1215    DOI: 10.1016/j.jia.2022.08.043
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Green manuring facilitates bacterial community dispersal across different compartments of subsequent tobacco

LIANG Hai1*, FU Li-bo2*, CHEN Hua2, ZHOU Guo-peng3, GAO Song-juan1#, CAO Wei-dong3# 

1 College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, P.R.China

2 Institute of Agricultural Environment and Resources, Yunnan Academy of Agricultural Sciences, Kunming 650205, P.R.China

3 Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

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绿肥-作物轮作作为一项可持续农艺措施,具有增强作物抗病、提升作物产量的效果。 然而,绿肥施用对作物相关微生物群落构建的调控机制尚不清楚。 本研究通过烟草与紫云英、黑麦草、肥田萝卜和冬(不施绿肥)四种轮作方式的田间试验,研究烟草不同区室(非根际土、根际土、根表及根内)细菌群落水平构建过程。 结果表明与冬闲处理相比,绿肥各处理相邻区室构建的共现网络具有更多的边,表明土壤-根界面存在显著的微生物相互作用。绿肥施用提高了土壤各区室间的扩散-生态位连续指数,促进了微生物的水平扩散。 对于不同的绿肥处理,中性群落模型对细菌出现频率的解释为24.6-27.6%,且每种处理至少有一个区室的归一化随机率高于50%边界,表明确定性和随机过程共同塑造了烟草微生物群落。综上所述,绿肥施用促进了不同区室间细菌群落的扩散,增强了相邻区室潜在的相互作用。本研究为了解绿肥-作物轮作条件下微生物群落的构建过程提供了经验依据。


Green manure–crop rotation is a sustainable approach to protect crops against diseases and improve yield.  However, the mechanism by which green manuring manipulates the crop-associated microbial community remains to be elucidated.  In this study, we explored the horizontal processes of bacterial communities in different compartments of the soil–root interface (bulk soil, rhizosphere soil, rhizoplane and endosphere) of tobacco by performing a field experiment including four rotation practices, namely, tobacco rotated with smooth vetch, ryegrass, radish, and winter fallow (without green manure).  Results showed that the co-occurrence networks constructed by adjacent compartments of the soil–root interface with green manuring had more edges than without green manuring, indicating  dramatic microbial interactions.  Green manuring increased the dispersal-niche continuum index between bulk soil and other compartments, indicating that it facilitated the horizontal dispersal of microbes.  For the different green manuring practices, the neutral community model explained 24.6–27.6% of detection frequency for bacteria, and at least one compartment under each practice had a normalized stochasticity ratio higher than the 50% boundary point, suggesting that the deterministic and stochastic processes jointly shaped the tobacco microbiome.  In conclusion, green manuring generally facilitates bacterial community dispersal across different compartments and enhances potential interactions among adjacent compartments.  This study provides empirical evidence for understanding the microbiome assembly under green manure–crop rotation.

Keywords:  green manure       tobacco       rotation practice         niche compartment         community assembly  
Received: 28 April 2022   Accepted: 13 June 2022
Fund: This work was supported financially by the National Key Research and Development Program of China (2021YFD1700200) and the China Agriculture Research System of MOF and MARA (CARS-22).
About author:  LIANG Hai, E-mail:; FU Li-bo, E-mail:; #Correspondence GAO Song-juan, E-mail:; CAO Wei-dong, E-mail: * These authors contributed equally to this study

Cite this article: 

LIANG Hai, FU Li-bo, CHEN Hua, ZHOU Guo-peng, GAO Song-juan, CAO Wei-dong. 2023. Green manuring facilitates bacterial community dispersal across different compartments of subsequent tobacco. Journal of Integrative Agriculture, 22(4): 1199-1215.

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