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Journal of Integrative Agriculture  2020, Vol. 19 Issue (3): 866-880    DOI: 10.1016/S2095-3119(19)62630-4
Special Issue: 农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe
Agro-ecosystem & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
A comprehensive analysis of the response of the fungal community structure to long-term continuous cropping in three typical upland crops
LIU Hang1, 2*, PAN Feng-juan1*, HAN Xiao-zeng1, SONG Feng-bin1, ZHANG Zhi-ming1, YAN Jun1, XU Yan-li1  
1 National Observation Station of Hailun Agro-ecology System/Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, P.R.China
2 University of Chinese Academy of Sciences, Beijing 100049, P.R.China
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Abstract  Certain agricultural management practices are known to affect the soil microbial community structure; however, knowledge of the response of the fungal community structure to the long-term continuous cropping and rotation of soybean, maize and wheat in the same agroecosystem is limited.  We assessed the fungal abundance, composition and diversity among soybean rotation, maize rotation and wheat rotation systems and among long-term continuous cropping systems of soybean, maize and wheat as the effect of crop types on fungal community structure.  We compared these fungal parameters of same crop between long-term crop rotation and continuous cropping systems as the effect of cropping systems on fungal community structure.  The fungal abundance and composition were measured by quantitative real-time PCR and Illumina MiSeq sequencing.  The results revealed that long-term continuous soybean cropping increased the soil fungal abundance compared with soybean rotation, and the fungal abundance was decreased in long-term continuous maize cropping compared with maize rotation.  The long-term continuous soybean cropping also exhibited increased soil fungal diversity.  The variation in the fungal community structure among the three crops was greater than that between long-term continuous cropping and rotation cropping.  Mortierella, Guehomyces and Alternaria were the most important contributors to the dissimilarity of the fungal communities between the continuous cropping and rotation cropping of soybean, maize and wheat.  There were 11 potential pathogen and 11 potential biocontrol fungi identified, and the relative abundance of most of the potential pathogenic fungi increased during the long-term continuous cropping of all three crops.  The relative abundance of most biocontrol fungi increased in long-term continuous soybean cropping but decreased in long-term continuous maize and wheat cropping.  Our results indicate that the response of the soil fungal community structure to long-term continuous cropping varies based upon crop types.
Keywords:  continuous cropping        crop rotation        fungal community structure        Illumina MiSeq sequencing  
Received: 31 October 2018   Accepted:
Fund: This study was funded by the National Key Research and Development Program of China (2016YFD02003009-6 and 2016YFD0300806), the National Natural Science Foundation of China (41771327 and 41571219) and the earmarked fund for China Agriculture Research System (CARS04).
Corresponding Authors:  Correspondence HAN Xiao-zeng, Tel: +86-451-86602940, Fax: +86-451-86603736, E-mail:    
About author:  LIU Hang, E-mail:; PAN Feng-juan, E-mail:; * These authors contributed equally to this study.

Cite this article: 

LIU Hang, PAN Feng-juan, HAN Xiao-zeng, SONG Feng-bin, ZHANG Zhi-ming, YAN Jun, XU Yan-li. 2020. A comprehensive analysis of the response of the fungal community structure to long-term continuous cropping in three typical upland crops. Journal of Integrative Agriculture, 19(3): 866-880.

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