Please wait a minute...
Journal of Integrative Agriculture  2015, Vol. 14 Issue (9): 1706-1714    DOI: 10.1016/S2095-3119(14)60936-9
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Identification of a novel male sterile wheat mutant dms conferring dwarf status and multi-pistils
 DUANZong-biao, SHENChun-cai, LIQiao-yun, LÜGui-zhen, NIYong-jing, YUDong-yan, NIUJi-shan
1、National Centre of Engineering and Technological Research for Wheat, Henan Agricultural University, Zhengzhou 450002,P.R.China
2、Shangqiu Academy of Agricultural and Forestry Sciences, Shangqiu 476000, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  Plant height and fertility are two important traits of wheat (Triticum aestivum L.), whose mutants are ideal materials for studies on molecular mechanisms of stem and floral organ development. In this study, we identified a dwarf, multi-pistil and male sterile (dms hereafter) wheat mutant from Zhoumai 18. Simple sequence repeat (SSR) marker assay with 181 primer pairs showed that only one locus of GWM148-2B was divergent between Zhoumai 18 and dms. There were three typical phenotypes in the progeny of dms, tall (T; ca. 0.8 m), semi-dwarf (M; ca. 0.6 m) and dwarf (D; under 0.3 m) plants. Morphological investigation indicated that the internode length of M was shortened by about 20–50 mm each; the internode number of D was 2 less than that of T and Zhoumai 18, and its internode length was shorter also. The pollen vigor and hybridization test demonstrated that dms mutant was male sterility. Segregated phenotypes in progeny of M suggested that the multi-pistils and sterility were controlled by one recessive gene locus which was designated as dms temporarily, and the plant height was controlled by a semi-dominant gene locus Dms. Therefore, progeny individuals of the dms had three genotypes, DmsDms for tall plants, Dmsdms for semi-dwarf plants and dmsdms for dwarf plants. The mutant progenies were individually selected and propagated for more than 6 generations, thus a set of near isogenic lines of T, M and D for dms were developed. This study provides a set germplasms for studies on molecular mechanisms of wheat stem and spike development.

Abstract  Plant height and fertility are two important traits of wheat (Triticum aestivum L.), whose mutants are ideal materials for studies on molecular mechanisms of stem and floral organ development. In this study, we identified a dwarf, multi-pistil and male sterile (dms hereafter) wheat mutant from Zhoumai 18. Simple sequence repeat (SSR) marker assay with 181 primer pairs showed that only one locus of GWM148-2B was divergent between Zhoumai 18 and dms. There were three typical phenotypes in the progeny of dms, tall (T; ca. 0.8 m), semi-dwarf (M; ca. 0.6 m) and dwarf (D; under 0.3 m) plants. Morphological investigation indicated that the internode length of M was shortened by about 20–50 mm each; the internode number of D was 2 less than that of T and Zhoumai 18, and its internode length was shorter also. The pollen vigor and hybridization test demonstrated that dms mutant was male sterility. Segregated phenotypes in progeny of M suggested that the multi-pistils and sterility were controlled by one recessive gene locus which was designated as dms temporarily, and the plant height was controlled by a semi-dominant gene locus Dms. Therefore, progeny individuals of the dms had three genotypes, DmsDms for tall plants, Dmsdms for semi-dwarf plants and dmsdms for dwarf plants. The mutant progenies were individually selected and propagated for more than 6 generations, thus a set of near isogenic lines of T, M and D for dms were developed. This study provides a set germplasms for studies on molecular mechanisms of wheat stem and spike development.
Keywords:  wheat       mutant       dwarf       multi-pistil       male sterility  
Received: 05 September 2014   Accepted:
Fund: 

This project was supported by the National 863 Program of China (2012AA101105) and the Henan Key Scientific and Technological Project, China (122101110200).

Corresponding Authors:  NIU Ji-shan, Tel: +86-371-63579687,Fax: +86-371-63558202, E-mail: jsniu@263.net     E-mail:  jsniu@263.net
About author:  DUAN Zong-biao, E-mail: zongbiaoduan@163.com;

Cite this article: 

DUANZong-biao , SHENChun-cai , LIQiao-yun , LüGui-zhen , NIYong-jing , YUDong-yan , NIUJi-shan . 2015. Identification of a novel male sterile wheat mutant dms conferring dwarf status and multi-pistils. Journal of Integrative Agriculture, 14(9): 1706-1714.

Bassam B J, Caetano-Anollés G, Gresshoff P M. 1991. Fastand sensitive silver staining of DNA in polyacrylamide gels.Analytical Biochemistry, 196, 80-83

Bhatia C R, Swaminathan M S. 1964. An induced multiple carpelmutation in bread wheat. Genetica, 34, 58-65

Chen J S, Zhang L H, Wu B L. 1983. A preliminary report onthe discovery and breeding of the “tri-grain wheat”, ActaAgronomica Sinica, 9, 67-71 (in Chinese)

Clark S E, Running M P, Meyerowitz E M. 1993. CLAVATA1,a regulator of meristem and flower development inArabidopsis. Development, 119, 397-418

Clark S E, Running M P, Meyerowitz E M. 1995. CLAVATA3 is aspecific regulator of shoot and floral meristem developmentaffecting the same processes as CLAVATA1. Development,121, 2057-2067

Cota-Sánchez J H, Remarchuk K, Ubayasena K. 2006. Readyto-use DNA extracted with a CTAB method adapted forherbarium specimens and mucilaginous plant tissue. PlantMolecular Biology Reporter, 24, 161-167

Fletcher J C. 2001. The ULTRAPETALA gene controls shootand floral meristem size in Arabidopsis. Development, 128,1323-1333

Gupta P, Balyan H, Edwards K, Isaac P, Korzun V, RöderM S, Gautier M F, Joudrer P, Schlatter A R, DubcovskyJ, Dela Pena R C, Khairallah M, Penner G, Hayden M J,Sharp P, Keller B, Wang R C, Hardouin J P, Jack P, LeroyP. 2002. Genetic mapping of 66 new microsatellite (SSR)loci in bread wheat. Theoretical and Applied Genetics, 105,413-422

Guyomarc’h H, Sourdille P, Edwards K, Rernard M. 2002.Studies of the transferability of microsatellites derived fromTriticum tauschii to hexaploid wheat and to diploid-relatedspecies using amplification, hybridization and sequencecomparisons. Theoretical and Applied Genetics, 105,736-744

Hama E, Takumi S, Ogihara Y, Murai K. 2004. Pistillody iscaused by alterations to the class-B MADS-box geneexpression pattern in alloplasmic wheats. Planta, 218,712-720

Jang S, Lee B, Kim C, Kim S J, Yim J, Han J J, Lee S,Kim S R, An G. 2003. The OsFOR1 gene encodes apolygalacturonase-inhibiting protein (PGIP) that regulatesfloral organ number in rice. Plant Molecular Biology, 53,357-369

Kayes J M, Clark S E. 1998. CLAVATA2, a regulator of meristemand organ development in Arabidopsis. Development, 125,3843-3851

Kim C, Jeong D H, An G. 2000. Molecular cloning andcharacterization of OsLRK1 encoding a putative receptorlikeprotein kinase from Oryza sativa. Plant Science, 152,17-26

Komaki M K, Okada K, Nishino E, Shimura Y. 1988. Isolation andcharacterization of novel mutants of Arabidopsis thalianadefective in flower development. Development, 104,195-203

Leyser H M O, Furner I J. 1992. Characterization of threeshoot apical meristem mutants of Arabidopsis thaliana.Development, 116, 397-403

Luo D P, Xu H, Liu Z L, Guo J X, Li H Y, Chen L T, Fang C,Zhang Q Y, Bai M, Yao N, Wu H, Wu H, Ji C H, ZhengH Q, Chen Y L, Ye S, Li X Y, Zhao X C, Li R Q, Liu YG. 2013. A detrimental mitochondrial-nuclear interactioncauses cytoplasmic male sterility in rice. Nature Genetics,45, 573-579

Ma S C, Zhang G S, Li H R, Zhao C S. 2006. Genetic analysisof multi-ovary character of wheat line Duo II. Journal ofTriticeae Crops, 26, 35-37 (in Chinese)

Ma S C, Zhang G S, Liu H W, Wang J W, Wang X L. 2000.Studies on the application of multi-ovary character to hybridwheat. I. Multi-ovary gene loci and cytoplasm effect. ActaBotanica Boreali-occidentalia Sinica, 20, 949-953 (inChinese)

Meguro A, Takumi S, Ogihara Y, Murai K. 2003. WAG, a wheatAGAMOUS homolog, is associated with development ofpistil-like stamens in alloplasmic wheats. Sexual PlantReproduction, 15, 221-230

Mizumoto K, Hatano H, Hirabayashi C, Murai K, Takumi S.2009. Altered expression of wheat AINTEGUMENTAhomolog, WANT-1, in pistil and pistil-like transformedstamen of an alloplasmic line with Aegilops crassacytoplasm. Development Genes and Evolution, 219,175-187

Murai K. 2013. Homeotic genes and the ABCDE model for floral organ formation in wheat. Plants, 2, 379-395

Murai K, Tsunewaki K. 1993. Photoperiod-sensitivecytoplasmic male sterility in wheat with Aegilops crassacytoplasm. Euphytica, 67, 41-48

Murai K, Takumi S, Koga H, Ogihara Y. 2002. Pistillody,homeotic transformation of stamens into pistil-likestructures, caused by nuclear-cytoplasm interaction inwheat. The Plant Journal, 29, 169-181

Nagasawa N, Miyoshi M, Kitano H, Satoh H, Nagato Y. 1996.Mutations associated with floral organ number in rice.Planta, 198, 627-633

Niu J S, Wang B Q, Wang Y H, Cao A Z, Qi Z J, Shen T M.2008. Chromosome location and microsatellite markerslinked to a powdery mildew resistance gene in wheat line‘Lankao 90 (6)’. Plant Breeding, 127, 346-349

Peng Z S, Martinek P, Kosuge K, Kuboyama T, Watanabe N.2008. Genetic mapping of a mutant gene producing threepistils per floret in common wheat. Journal of AppliedGenetics, 49, 135-139

Peng Z S, Yang J, Wei S H, Zeng J H. 2004. Characterizationof the common wheat (Triticum aestivum L.) mutation lineproducing three pistils in a floret. Hereditas, 141, 15-18

Peng Z S, Yang Z, Ouyang Z, Yang H. 2013. Characterizationof a novel pistillody mutant in common wheat. AustralianJournal of Crop Science, 7, 159-164

Pestsova E, Ganal M W, Röder M S. 2000. Isolation andmapping of microsatellite markers specific for the D genomeof bread wheat. Genome, 43, 689-697

Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal M W. 1998. A microsatellite map ofwheat. Genetics, 149, 2007-2023

Running M P, Meyerowitz E M. 1996. Mutations in thePERIANTHIA gene of Arabidopsis specifically alter floralorgan number and initiation pattern. Development, 122,1261-1269

Saraike T, Shitsukawa N, Yamamoto Y, Hagita H, Iwasaki Y,Takumi S, Murai K. 2007. Identification of a protein kinasegene associated with pistillody, homeotic transformationof stamens into pistil-like structures, in alloplasmicwheat. Planta, 227, 211-221

Shen G H, Tong Y Z, Shen G Z. 1992. Localization of thegene multi-ovary on chromosome and chromosome-arm ofcommon wheat using monosomic and ditelosomic analysis.Acta Genetica Sinica, 19, 513-516 (in Chinese)

Somers D J, Isaac P, Edwards K. 2004. A high-densitymicrosatellite consensus map for bread wheat (Triticumaestivum L.). Theoretical and Applied Genetics, 109,1105-1114

Song Q J, Shi J R, Singh S, Fickus E W, Costa J M, LewisJ, Cregan P B. 2005. Development and mapping ofmicrosatellite (SSR) markers in wheat. Theoretical andApplied Genetics, 110, 550-560

Sourdille P, Cadalen T, Guyomarc’h H, Snape J W J W,Perretant M R M R, Charmet G, Bernard M. 2003. An updateof the Courtot×Chinese Spring intervarietal molecularmarker linkage map for the QTL detection of agronomictraits in wheat. Theoretical and Applied Genetics, 106,530-538

Stell R G D, Torrie J H, Dickey D A. 1980. Principles andProcedures of Statistics: A Biometrical Approach.MacGraw-Hill, New York.Suzaki T, Sato M, Ashikari M, Miyoshi M, Nagato Y, Hirano HY. 2004. The gene FLORAL ORGAN NUMBER1 regulatesfloral meristem size in rice and encodes a leucine-rich repeatreceptor kinase orthologous to Arabidopsis CLAVATA1.Development, 131, 5649-5657

Taguchi-Shiobara F, Yuan Z, Hake S, Jackson D. 2001. Thefasciated ear 2 gene encodes a leucine-rich repeat receptorlikeprotein that regulates shoot meristem proliferation inmaize. Genes & Development, 15, 2755-2766

Theissen G, Saedler H. 1999. The golden decade ofmolecular floral development (1990-1999): A cheerfulobituary

 Developmental Genetics, 25, 181-193

Tong Y Z, Tong P D. 1984. Studies on multi-ovary in commonwheat. I. The morphologenesis of multi-ovary in commonwheat and the chromosomal location of its genes. Journal ofShanghai Normal University (Natural Sciences), 2, 48-53(in Chinese)

Vahamidis P, Karamanos A, Economou G, Fasseas C.2014. A new scale for the assessment of wheat spikemorphogenesis. Annals of Applied Biology, 164, 220-231

Wang Y Z, Ding H B, Chen C, Chen J S. 1991. The developmentand abnormality of embryo and endosperm in trigrain wheat.Acta Botanica Sinica, 33, 176-180 (in Chinese)

Wang Y Z, Ding H B, Jin Z L. 1989. Initiation and developmentof flowers in a multi-pistil wheat. Acta Botanica BorealioccidentaliaSinica (China), 9, 131-135 (in Chinese)

Wang Z G, Xu D H, Ji J, Wang J, Wang M C, Ling H Q, Sun GL, Li J M. 2009. Genetic analysis and molecular markersassociated with multi-gynoecia (Mg) gene in Trigrainwheat. Canadian Journal of Plant Science, 89, 845-850

Wu J, Li B Q, Zhao J X. 2000. Genetic analysis of multi-ovarycharacter of trigrain wheat. The Journal of NorthwestAgricultural University, 28, 58-60 (in Chinese)

Xi Y J, Ma X F, Zhong H, Liu S D, Wang Z L, Song Y Y, ZhaoC H. 2011. Characterization of a male sterile mutant fromprogeny of a transgenic plant containing a leaf senescenceinhibitiongene in wheat. Euphytica, 177, 241-251

Yamada K, Saraike T, Shitsukawa N, Hirabayashi C, TakumiS, Murai K. 2009. Class D and Bsister MADS-box genesare associated with ectopic ovule formation in the pistil-likestamens of alloplasmic wheat (Triticum aestivum L.). PlantMolecular Biology, 71, 1-14

Zhu Y, Saraike T, Yamamoto Y, Hagita H, Takumi S, Murai K.2008. Orf260cra, a novel mitochondrial gene, is associatedwith the homeotic transformation of stamens into pistil-likestructures (pistillody) in alloplasmic wheat. Plant and CellPhysiology, 49, 1723-1733
[1] CHU Jin-peng, GUO Xin-hu, ZHENG Fei-na, ZHANG Xiu, DAI Xing-long, HE Ming-rong. Effect of delayed sowing on grain number, grain weight, and protein concentration of wheat grains at specific positions within spikes[J]. >Journal of Integrative Agriculture, 2023, 22(8): 2359-2369.
[2] GUO Bao-jian, SUN Hong-wei, QI Jiang, HUANG Xin-yu, HONG Yi, HOU Jian, LÜ Chao, WANG Yu-lin, WANG Fei-fei, ZHU Juan, GUO Gang-gang, XU Ru-gen. A single nucleotide substitution in the MATE transporter gene regulates plastochron and many noded dwarf phenotype in barley (Hordeum vulgare L.)[J]. >Journal of Integrative Agriculture, 2023, 22(8): 2295-2305.
[3] WANG Xue-feng, SHAO Dong-nan, LIANG Qian, FENG Xiao-kang, ZHU Qian-hao, YANG Yong-lin, LIU Feng, ZHANG Xin-yu, LI Yan-jun, SUN Jie, XUE Fei. A 2-bp frameshift deletion at GhDR, which encodes a B-BOX protein that co-segregates with the dwarf-red phenotype in Gossypium hirsutum L.[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2000-2014.
[4] FAN Ting-lu, LI Shang-zhong, ZHAO Gang, WANG Shu-ying, ZHANG Jian-jun, WANG Lei, DANG Yi, CHENG Wan-li. Response of dryland crops to climate change and drought-resistant and water-suitable planting technology: A case of spring maize[J]. >Journal of Integrative Agriculture, 2023, 22(7): 2067-2079.
[5] WU Xian-xin, ZANG Chao-qun, ZHANG Ya-zhao, XU Yi-wei, WANG Shu, LI Tian-ya, GAO Li.

Characterization of wheat monogenic lines with known Sr genes and wheat cultivars for resistance to three new races of Puccinia graminis f. sp. tritici in China [J]. >Journal of Integrative Agriculture, 2023, 22(6): 1740-1749.

[6] TANG Chan-juan, LUO Ming-zhao, ZHANG Shuo, JIA Guan-qing, TANG Sha, JIA Yan-chao, ZHI Hui, DIAO Xian-min. Variations in chlorophyll content, stomatal conductance and photosynthesis in Setaria EMS mutants[J]. >Journal of Integrative Agriculture, 2023, 22(6): 1618-1630.
[7] ZHANG Chong, WANG Dan-dan, ZHAO Yong-jian, XIAO Yu-lin, CHEN Huan-xuan, LIU He-pu, FENG Li-yuan, YU Chang-hao, JU Xiao-tang. Significant reduction of ammonia emissions while increasing crop yields using the 4R nutrient stewardship in an intensive cropping system[J]. >Journal of Integrative Agriculture, 2023, 22(6): 1883-1895.
[8] DU Xiang-bei, XI Min, WEI Zhi, CHEN Xiao-fei, WU Wen-ge, KONG Ling-cong. Raised bed planting promotes grain number per spike in wheat grown after rice by improving spike differentiation and enhancing photosynthetic capacity[J]. >Journal of Integrative Agriculture, 2023, 22(6): 1631-1644.
[9] ZHANG Zhen-zhen, CHENG Shuang, FAN Peng, ZHOU Nian-bing, XING Zhi-peng, HU Ya-jie, XU Fang-fu, GUO Bao-wei, WEI Hai-yan, ZHANG Hong-cheng. Effects of sowing date and ecological points on yield and the temperature and radiation resources of semi-winter wheat[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1366-1380.
[10] LI Jiao-jiao, ZHAO Li, LÜ Bo-ya, FU Yu, ZHANG Shu-fa, LIU Shu-hui, YANG Qun-hui, WU Jun, LI Jia-chuang, CHEN Xin-hong. Development and characterization of a novel common wheat–Mexico Rye T1DL·1RS translocation line with stripe rust and powdery mildew resistance[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1291-1307.
[11] DONG Xiu-chun, QIAN Tai-feng, CHU Jin-peng, ZHANG Xiu, LIU Yun-jing, DAI Xing-long, HE Ming-rong. Late sowing enhances lodging resistance of wheat plants by improving the biosynthesis and accumulation of lignin and cellulose[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1351-1365.
[12] ZHAO Xiao-dong, QIN Xiao-rui, LI Ting-liang, CAO Han-bing, XIE Ying-he. Effects of planting patterns plastic film mulching on soil temperature, moisture, functional bacteria and yield of winter wheat in the Loess Plateau of China[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1560-1573.
[13] JIANG Yun, WANG De-li, HAO Ming, ZHANG Jie, LIU Deng-cai.

Development and characterization of wheat–Aegilops kotschyi 1Uk(1A) substitution line with positive dough quality parameters [J]. >Journal of Integrative Agriculture, 2023, 22(4): 999-1008.

[14] TU Ke-ling, YIN Yu-lin, YANG Li-ming, WANG Jian-hua, SUN Qun. Discrimination of individual seed viability by using the oxygen consumption technique and headspace-gas chromatography-ion mobility spectrometry[J]. >Journal of Integrative Agriculture, 2023, 22(3): 727-737.
[15] Sunusi Amin ABUBAKAR, Abdoul Kader Mounkaila HAMANI, WANG Guang-shuai, LIU Hao, Faisal MEHMOOD, Abubakar Sadiq ABDULLAHI, GAO Yang, DUAN Ai-wang. Growth and nitrogen productivity of drip-irrigated winter wheat under different nitrogen fertigation strategies in the North China Plain[J]. >Journal of Integrative Agriculture, 2023, 22(3): 908-922.
No Suggested Reading articles found!