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Journal of Integrative Agriculture  2015, Vol. 14 Issue (4): 691-697    DOI: 10.1016/S2095-3119(14)60800-5
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Inhibitory effect of chitosan on growth of the fungal phytopathogen, Sclerotinia sclerotiorum, and sclerotinia rot of carrot
WANG Qing, ZUO Jin-hua, WANG Qian, NA Yang, GAO Li-pu
Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Fruits and Vegetable Storage and Processing/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture/Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing 100097, P.R.China
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摘要  The antifungal activity of chitosan on a common fungal phytopathogen, Sclerotinia sclerotiorum, and the control effect on sclerotinia rot of carrot were investigated. Mycelial growth and fungal biomass were strongly inhibited by chitosan. Using propidium iodide stain combined with fluorescent microscopy, the plasma membrane of chitosan-treated S. sclerotiorum mycelia was observed to be markedly damaged. Concomitantly, protein leakage and lipid peroxidation was also found to be significantly higher in chitosan-treated mycelia compared to the control. Chitosan provided an effective control of sclerotinia rot of carrot, with induction of activity of defense-related enzymes including polyphenoloxidase and peroxidase. These data suggest that the effects of chitosan on sclerotinia rot of carrot may be associated with the direct damage to the plasma membrane and lipid peroxidation of S. sclerotiorum, and the elicitation of defense response in carrot.

Abstract  The antifungal activity of chitosan on a common fungal phytopathogen, Sclerotinia sclerotiorum, and the control effect on sclerotinia rot of carrot were investigated. Mycelial growth and fungal biomass were strongly inhibited by chitosan. Using propidium iodide stain combined with fluorescent microscopy, the plasma membrane of chitosan-treated S. sclerotiorum mycelia was observed to be markedly damaged. Concomitantly, protein leakage and lipid peroxidation was also found to be significantly higher in chitosan-treated mycelia compared to the control. Chitosan provided an effective control of sclerotinia rot of carrot, with induction of activity of defense-related enzymes including polyphenoloxidase and peroxidase. These data suggest that the effects of chitosan on sclerotinia rot of carrot may be associated with the direct damage to the plasma membrane and lipid peroxidation of S. sclerotiorum, and the elicitation of defense response in carrot.
Keywords:  antifungal activity       carrot       chitosan       plasma membrane       Sclerotinia sclerotiorum  
Received: 31 March 2014   Accepted: 10 April 2015
Fund: 

This research was supported by grants from the National Natural Science Foundation of China (31101364), the Ministry of Agriculture of China (CARS-25-E-01 and 201203095) and the Beijing Academy of Agriculture and Forestry Sciences, China (CXJJ201304).

Corresponding Authors:  GAO Li-pu, Tel/Fax: +86-10-51503051,E-mail: gaolipu@nercv.org     E-mail:  gaolipu@nercv.org
About author:  WANG Qing, E-mail: wangqing@nercv.org;

Cite this article: 

WANG Qing, ZUO Jin-hua, WANG Qian, NA Yang, GAO Li-pu. 2015. Inhibitory effect of chitosan on growth of the fungal phytopathogen, Sclerotinia sclerotiorum, and sclerotinia rot of carrot. Journal of Integrative Agriculture, 14(4): 691-697.

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