|
|
|
|
|
| Essential Oil from Inula britannica Extraction with SF-CO2 and Its Antifungal Activity |
| ZHAO Te, GAO Fei, ZHOU Lin, SONG Tian-you |
1 Henan Key Laboratory for Creation and Application of New Pesticides, Zhengzhou 450002, P.R.China
2 College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, P.R.China |
|
|
|
摘要 The aim of this study was to determine the extraction technique of supercritical fluid carbon dioxide (SF-CO2) for the essential oil from Inula britannica flowers and its antifungal activities against plant pathogenic fungi for its potential application as botanical fungicide. The effects of factors, including extraction temperature, extraction pressure, SF-CO2 flow rate, flower powder size, and time on the essential oil yield were studied using the single factor experiment. An orthogonal experiment was conducted to determine the best operating conditions for the maximum extraction oil yield. Adopting the optimum conditions, the maximum yield reached 10.01% at 40°C temperature, 30 MPa pressure, 60 mesh flower powder size, 20 L h-1 SF-CO2 flow rate, and 90 min extraction time. The antifungal activities of I. britannica essential oil using the SF-CO2 against the most important plant pathogenic fungi were also examined through in vitro and in vivo tests. Sixteen plant pathogenic fungi were inhibited to varying degrees at 1 mg mL-1 concentration of the essential oil. The mycelial growth of Gaeumannomyces graminis var. tritici was completely inhibited. The radial growths of Phytophthora capsici and Fusarium monilifome were also inhibited by 83.76 and 64.69%, respectively. In addition, the essential oil can inhibit the spore germination of Fusarium oxysporum f. sp. vasinfectum, Phytophthora capsici, Colletotrichum orbiculare, and Pyricularia grisea, and the corresponding inhibition rates were 98.26, 96.54, 87.89, and 87.35% respectively. The present study has demonstrated that the essential oil of I. britannica flowers extracted through the SF-CO2 technique is one potential and promising antifungal agent that can be used as botanical fungicide to protect crops.
Abstract The aim of this study was to determine the extraction technique of supercritical fluid carbon dioxide (SF-CO2) for the essential oil from Inula britannica flowers and its antifungal activities against plant pathogenic fungi for its potential application as botanical fungicide. The effects of factors, including extraction temperature, extraction pressure, SF-CO2 flow rate, flower powder size, and time on the essential oil yield were studied using the single factor experiment. An orthogonal experiment was conducted to determine the best operating conditions for the maximum extraction oil yield. Adopting the optimum conditions, the maximum yield reached 10.01% at 40°C temperature, 30 MPa pressure, 60 mesh flower powder size, 20 L h-1 SF-CO2 flow rate, and 90 min extraction time. The antifungal activities of I. britannica essential oil using the SF-CO2 against the most important plant pathogenic fungi were also examined through in vitro and in vivo tests. Sixteen plant pathogenic fungi were inhibited to varying degrees at 1 mg mL-1 concentration of the essential oil. The mycelial growth of Gaeumannomyces graminis var. tritici was completely inhibited. The radial growths of Phytophthora capsici and Fusarium monilifome were also inhibited by 83.76 and 64.69%, respectively. In addition, the essential oil can inhibit the spore germination of Fusarium oxysporum f. sp. vasinfectum, Phytophthora capsici, Colletotrichum orbiculare, and Pyricularia grisea, and the corresponding inhibition rates were 98.26, 96.54, 87.89, and 87.35% respectively. The present study has demonstrated that the essential oil of I. britannica flowers extracted through the SF-CO2 technique is one potential and promising antifungal agent that can be used as botanical fungicide to protect crops.
|
|
Received: 27 September 2012
Accepted: 01 October 2013
|
| Fund: The study was financially supported by the Scientific and Technological Key Project of Henan Province, China (082102350006 and 102102310242), and the College Young Teachers Projects of Henan Province, China (2010GGJS- 046). |
|
Corresponding Authors:
Correspondence ZHOU Lin, Tel: +86-371-63558170, Mobile: 13643851328, Fax: +86-371-63558170, E-mail: zhoulin@henau.edu.cn
E-mail: zhoulin@henau.edu.cn
|
| About author: ZHAO Te, E-mail: tezhao@126.com |
Cite this article:
ZHAO Te, GAO Fei, ZHOU Lin, SONG Tian-you.
2013.
Essential Oil from Inula britannica Extraction with SF-CO2 and Its Antifungal Activity. Journal of Integrative Agriculture, 12(10): 1791-1798.
|
| [1]Aleksovski S A, Sovová H. 2007. Supercritical CO2 extraction of Salvia officinalis L. The Journal of Supercritical Fluid, 40, 239-245[2]Campanile F, Villecco D, Zaccardelli M. 2009. Use of essential oils of thyme and oregano against phytopathogenic fungi and bacteria. Journal of Plant Pathology, 91(Special Issue), 89-96[3]Chang H T, Cheng Y H, Wu C L, Chang S T, Chang T T, Su Y C. 2008. Antifungal activity of essential oil and its constituents from Calocedrus macrolepis var. formosana florin leaf against plant pathogenic fungi. Bioresource Technology, 99, 6266-6270[4]de Corato U, Trupo M, Leone G P, Di Sanzo G, Zingarelli G, Adami M. 2007. Antifungal activity of the leaf extracts of laurel (Laurus nobilis L.), sweet orange (Citrus sinensis Osbeck) and olive (Olea europaea L.) obtained by means of supercritical carbon dioxide technique. Journal of Plant Pathology, 89(Special Issue), 54-60[5]Daferera D J, Ziogas B N, Polissiou M G. 2003. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis. Crop Protection, 22, 39-44[6]Deans S G, Svoboda K P, Kennedy A I. 1989. Biological activity of plant volatile oils and their constituents. Planta Medica, 55, 588. [7]Ding H X, Li G Z, Feng J T, Zhang X. 2005. Anti-fungi active compounds from Inula britannica. Journal of Northwest Sci-Tech University of Agriculture and Forestry (Natural Science Edition), 33, 90-94[8](in Chinese) Duke S O. 1990. Natural pesticides from plants. In: Janick J, Simon J E, eds., Advances in New Crops. Timber Press, Portland, OR. pp. 511-517[9]Gigante B, Silva A M, Marcelo Curto M J, Savluchinske Feio S, Roseiro J C, Reis V R. 2002. Structural effects on the bioactivity of dehydroabietic acid derivatives. Planta Medica, 68, 680-684[10]Isman B M. 2000. Plant essential oils for pest and disease management. Crop Protection, 19, 603-608[11]Jiao B, Zhou L, Song T Y, Wang H J, You X F, Sun S J. 2010. Antifungal activity study of flower chloroform extract of Inula britannica L. Journal of Henan Agricultural Science, 38, 60-62, 65[12]Lee A K K, Bulley N R, Fattori M, Meisen A. 1986, Modeling of supercirital carbon dioxide extraction of canola oil seeds in fixed bed. Journal of the Amercia Oil Chemists Society, 63, 921-925[13]Lee Y S, Kim J, Shin S C, Lee S G, Park I K. 2008. Antifungal activity of Myrtaceae essential oils and their components against three phytopathogenic fungi. Flavour Fragrance Journal, 23, 23-28[14]Luu T D, Raffaella M, Paul T, Neil F. 2009. Response surface method applied to supercritical carbon dioxide extraction of Vetiveria zizanioides essential oil. Chemical Engineering Journal, 155, 617-626[15]Mandana B, Russly A R, Farah S T, Ali G, Liza M S, Jinap S, Azizah H, Zaidul I S M. 2011. Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food Bioprod Process, 89, 67-72[16]Mu L Y. 1994. Research methods of plant chemical protection. China Agriculture Press, Beijing. pp. 72-82[17](in Chinese) Mukhopadhyay M. 2000. Natural Extracts Using SuperCritical Carbon Dioxide. CRC Press, Boca Raton. p. 339. Nanci P P, Marcia O M M, Meireles M A A. 2001. Supercritical CO2 extraction of essential oil and oleoresin from chamomile (Chamomilla recutita [L.] Rauschert). Journal of Supercritical Fluid, 21, 245-256[18]Pandey V N, Dubey N K. 1994. Antifungal potential of leaves and essential oils from higher plants against soil phytopathogens. Soil Biology and Biochemistry, 26, 1417-1421[19]Reverchon E. 1997. Supercritical fluid extraction and fractionation of essential oils and related products. Journal of Supercritical Fluid, 10, 1-37[20]Singh A K, Dikshit A, Sharma M L, Dixit S N. 1980. Fungitoxic activity of some essential oils. Economic Botany, 34, 186-190[21]Sthal E, Gerard D. 1985. Solubility behaviour and fractionation of essential oils in dense carbon dioxide. Perfum Flavor, 10, 29-37[22]Ugo DC, Oliviero M, Mario T, Giuseppe D S. 2010. Use of essential oil of Laurus nobilis obtained by means of a supercritical carbon dioxide technique against post harvest spoilage fungi. Crop Protection, 29, 142-147[23]Wang J Q, Xu W Y, Zhu Q L, Wu G. 2006. Control efficacy of 56% cupric hydroxide•dimethomorph WP against phytophthora blight in pepper plants. Subtropical Agriculture Research, 2, 37-40[24]Wu Y B, Zhang D Q, Wang Y Z. 2006. Advances in the study of chemical constituents of Inula britannica. Natural Product Research and Development, 18, 503- 507. Yang D, Liu H Y, Gao W W, Chen S L. 2010. Activities of essential oils from Asarum heterotropoides var. mandshuricum against five phytopathogens. Crop Protection, 29, 295-299[25]Zambonelli A, Bianchi A, Curini M, Epifano F, Bruni R, Iotti M. November 2003. Effetto degli oli essenziali di Erigeron canadensise Myrtus communis sullo sviluppo di funghi fitopatogeni in vitro. In: Proceedings of the Conference of AIPP, Sanremo, Italy. pp. 27-28[26](in Italian) Zha J P, Fu Y. 2005. Study of chemical constituents of the essential oil from Inula britannica L. by GC-MS. Journal of Chinese Medicine Material, 28, 466-468[27]Zhou L, Jiao B, Gao F, Song T Y, Zhang X. 2010. Antifungal activities of flower extracts of Inula britannica L. with different solvents. Journal of Zhejiang University (Agriculture and Life Sciences), 36, 287-292. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
