Please wait a minute...
Journal of Integrative Agriculture  2015, Vol. 14 Issue (2): 389-397    DOI: 10.1016/S2095-3119(14)60772-3
Soil & Fertilization · Irrigation · Agro-Ecology & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Inoculation with chlamydospores of Trichoderma asperellum SM- 12F1 accelerated arsenic volatilization and influenced arsenic availability in soils
 WANG Xiu-rong, SU Shi-ming, ZENG Xi-bai, BAI Ling-yu, LI Lian-fang, DUAN Ran, WANG Ya-nan, WU Cui-xia
Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Environment, Ministry of Agriculture, Beijing 100081, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  Fungi capable of arsenic (As) accumulation and volatilization are hoped to tackle As-contaminated environment in the future. However, little data is available regarding their performances in field soils. In this study, the chlamydospores of Trichoderma asperellum SM-12F1 capable of As resistance, accumulation, and volatilization were inoculated into As-contaminated Chenzhou (CZ) and Shimen (SM) soils, and subsequently As volatilization and availability were assessed. The results indicated that T. asperellum SM-12F1 could reproduce well in As-contaminated soils. After cultivated for 42 days, the colony forming units (cfu) of T. asperellum SM-12F1 in CZ and SM soils reached 1010–1011 cfu g–1 fresh soil when inoculated at a rate of 5.0%. Inoculation with chlamydospores of T. asperellum SM-12F1 could significantly accelerate As volatilization from soils. The contents of volatilized As from CZ and SM soils after being inoculated with chlamydospores at a rate of 5.0% for 42 days were 2.0 and 0.6 μg kg–1, respectively, which were about 27.5 and 2.5 times higher than their corresponding controls of no inoculation (CZ, 0.1 μg kg–1; SM, 0.3 μg kg–1). Furthermore, the available As content in SM soils was decreased by 23.7%, and that in CZ soils increased by 3.3% compared with their corresponding controls. Further studies showed that soil pH values significantly decreased as a function of cultivation time or the inoculation level of chlamydospores. The pH values in CZ and SM soils after being inoculated with 5.0% of chlamydospores for 42 days were 6.04 and 6.02, respectively, which were lowered by 0.34 and 1.21 compared with their corresponding controls (CZ, 6.38; SM, 7.23). The changes in soil pH and As-binding fractions after inoculation might be responsible for the changes in As availability. These observations could shed light on the future remediation of As-contaminated soils using fungi.

Abstract  Fungi capable of arsenic (As) accumulation and volatilization are hoped to tackle As-contaminated environment in the future. However, little data is available regarding their performances in field soils. In this study, the chlamydospores of Trichoderma asperellum SM-12F1 capable of As resistance, accumulation, and volatilization were inoculated into As-contaminated Chenzhou (CZ) and Shimen (SM) soils, and subsequently As volatilization and availability were assessed. The results indicated that T. asperellum SM-12F1 could reproduce well in As-contaminated soils. After cultivated for 42 days, the colony forming units (cfu) of T. asperellum SM-12F1 in CZ and SM soils reached 1010–1011 cfu g–1 fresh soil when inoculated at a rate of 5.0%. Inoculation with chlamydospores of T. asperellum SM-12F1 could significantly accelerate As volatilization from soils. The contents of volatilized As from CZ and SM soils after being inoculated with chlamydospores at a rate of 5.0% for 42 days were 2.0 and 0.6 μg kg–1, respectively, which were about 27.5 and 2.5 times higher than their corresponding controls of no inoculation (CZ, 0.1 μg kg–1; SM, 0.3 μg kg–1). Furthermore, the available As content in SM soils was decreased by 23.7%, and that in CZ soils increased by 3.3% compared with their corresponding controls. Further studies showed that soil pH values significantly decreased as a function of cultivation time or the inoculation level of chlamydospores. The pH values in CZ and SM soils after being inoculated with 5.0% of chlamydospores for 42 days were 6.04 and 6.02, respectively, which were lowered by 0.34 and 1.21 compared with their corresponding controls (CZ, 6.38; SM, 7.23). The changes in soil pH and As-binding fractions after inoculation might be responsible for the changes in As availability. These observations could shed light on the future remediation of As-contaminated soils using fungi.
Keywords:  arsenic       volatilization       availability       soil       chlamydospores       T. asperellum SM-12F1  
Received: 30 November 2013   Accepted:
Fund: 

the financial support from the National Natural Science Foundation of China (41101296), and the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2012BAD14B02).

Corresponding Authors:  ZENG Xi-bai,Tel: +86-10-82105612, E-mail: zengxibai@caas.cn     E-mail:  zengxibai@caas.cn
About author:  WANG Xiu-rong, Tel: +86-10-82106009, E-mail: 124922870wxr@ sina.com; SU Shi-ming, Tel: +86-10-82106009, E-mail: sushiming@caas.cn; * These authors contributed equally to this study.

Cite this article: 

WANG Xiu-rong, SU Shi-ming, ZENG Xi-bai, BAI Ling-yu, LI Lian-fang, DUAN Ran, WANG Ya-nan, WU Cui-xia. 2015. Inoculation with chlamydospores of Trichoderma asperellum SM- 12F1 accelerated arsenic volatilization and influenced arsenic availability in soils. Journal of Integrative Agriculture, 14(2): 389-397.

Achal V, Pan X L, Fu Q L, Zhang D Y. 2012. Biomineralizationbased remediation of As (III) contaminated soil bySporosarcina ginsengisoli. Journal of Hazardous Materials,201, 178-184

?erňanský S, Kolen?ík M, Ševc J, Urík M, Hiller E 2009.Fungal volatilization of trivalent and pentavalent arsenicunder laboratory conditions. Bioresource Technology, 100,1037-1040

Dixit S, Hering J G. 2003. Comparison of arsenic (V) andarsenic (III) sorption onto iron oxide minerals: Implicationsfor arsenic mobility. Environmental Science & Technology,37, 4182-4189

Edvantoro B B, Naidu R, Megharaj M, Merrington G, SingletonI. 2004. Microbial formation of volatile arsenic in cattle dipsite soils contaminated with arsenic and DDT. Applied SoilEcology, 25, 207-217

Elad Y, Chet I. 1983. Improved selective media for isolationof Trichoderma spp. or Fusarium spp. Phytoparasitica,11, 55-58

Fayiga A O, Ma L Q. 2006. Using phosphate rock toimmobilize metals in soil and increase arsenic uptakeby hyperaccumulator Pteris vittata. Science of the TotalEnvironment, 359, 17-25

Gadd G M. 2004. Microbial influence on metal mobility andapplication for bioremediation. Geoderma, 122, 109-119

Huang H, Jia Y, Sun G X, Zhu Y G. 2012. Arsenic speciationand volatilization from flooded paddy soils amendedwith different organic matters. Environmental Science &Technology, 46, 2163-2168

Huang J H, Hu K N, Decker B. 2011. Organic arsenic in thesoil environment: speciation, occurrence, transformation,and adsorption behavior. Water, Air, & Soil Pollution, 219,401-415

Lewis J, Papavizas G. 1983. Production of chlamydosporesand conidia by Trichoderma spp. in liquid and solid growthmedia. Soil Biology and Biochemistry, 15, 351-357

Liu S, Zhang F, Chen J, Sun G X. 2011. Arsenic removalfrom contaminated soil via biovolatilization by geneticallyengineered bacteria under laboratory conditions. Journalof Environmental Sciences, 23, 1544-1550

Mestrot A, Feldmann J, Krupp E M, Hossain M S, Roman-RossG, Meharg A A. 2011. Field fluxes and speciation of arsinesemanating from soils. Environmental Science & Technology,45, 1798-1804

Mestrot A, Uroic M K, Plantevin T, Islam M R, Krupp E M,Feldmann J R, Meharg A A. 2009. Quantitative and qualitative trapping of arsines deployed to assess loss ofvolatile arsenic from paddy soil. Environmental Science &Technology, 43, 8270-8275

Papavizas G. 1982. Survival of Trichoderma harzianum in soiland in pea and bean rhizospheres. Phytopathology, 72,121-125

Pokhrel D, Viraraghvavan T. 2008. Arsenic removal from anaqueous solution by modified A. niger biomass: Batchkinetic and isotherm studies. Journal of HazardousMaterials, 150, 818-825

Qin J, Lehr C R, Yuan C, Le X C, McDermott T R, Rosen BP. 2009. Biotransformation of arsenic by a Yellowstonethermoacidophilic eukaryotic alga. Proceedings of theNational Academy of Sciences of the United States ofAmerica, 106, 5213-5217

Sneh B, Dupler M, Elad Y, Baker R. 1984. Chlamydosporegermination of Fusarium oxysporum f. sp. cucumerinum asaffected by fluorescent and lytic bacteria from a Fusariumsuppressivesoil. Phytopathology, 74, 1115-1124

Srivastava P K, Vaish A, Dwivedi S, Chakrabarty D, Singh N,Tripathi R D. 2011. Biological removal of arsenic pollutionby soil fungi. Science of the Total Environment, 409,2430-2442

Su S M, Zeng X B, Bai L Y, Li L F, Duan R. 2011. Arsenicbiotransformation by arsenic-resistant fungi Trichodermaasperellum SM-12F1, Penicillium janthinellum SM-12F4,and Fusarium oxysporum CZ-8F1. Science of the TotalEnvironment, 409, 5057-5062

Su S M, Zeng X B, Jiang X L, Li L F. 2010. Bioaccumulationand biovolatilisation of pentavalent arsenic by Penicillinjanthinellum, Fusarium oxysporum and Trichodermaasperellum under laboratory conditions. CurrentMicrobiology, 61, 261-266

Su S M, Zeng X B, Li L F, Duan R, Bai L Y, Li A G, Wang J,Jiang S. 2012. Arsenate reduction and methylation in thecells of Trichoderma asperellum SM-12F1, Penicilliumjanthinellum SM-12F4, and Fusarium oxysporum CZ-8F1investigated with X-ray absorption near edge structure.Journal of Hazardous Materials, 243, 364-367

Tabak H H, Lens P, van Hullebusch E D, Dejonghe W. 2005.Developments in bioremediation of soils and sedimentspolluted with metals and radionuclides -1 Microbialprocesses and mechanisms affecting bioremediation ofmetal contamination and influencing metal toxicity andtransport. Reviews in Environmental Science and Bio/Technology, 4, 115-156

Wang P P, Sun G X, Jia Y, Meharg A A, Zhu Y G. 2013.A review on completing arsenic biogeochemical cycle:Microbial volatilization of arsines in environment. Journalof Environmental Sciences, 26, 371-381

Wang S L, Zhao X Y. 2009. On the potential of biologicaltreatment for arsenic contaminated soils and groundwater.Journal of Environmental Management, 90, 2367-2376

Wenzel W W, Kirchbaumer N, Prohaska T, Stingeder G, LombiE, Adriano D C. 2001. Arsenic fractionation in soils usingan improved sequential extraction procedure. AnalyticaChimica Acta, 436, 309-323

Woolson E, Axley J, Kearney P. 1971. Correlation betweenavailable soil arsenic, estimated by six methods, andresponse of corn (Zea mays L.). Soil Science Society ofAmerica Journal, 35, 101-105

Ye J, Rensing C, Rosen B P, Zhu Y G. 2012. Arsenicbiomethylation by photosynthetic organisms. Trends inPlant Science, 17, 155-162

Zeng X B, Su S M, Jiang X L, Li L F, Bai L Y, Zhang Y R. 2010.Capability of pentavalent arsenic bioaccumulation andbiovolatilization of three fungal strains under laboratoryconditions. CLEAN-Soil, Air, Water, 38, 238-241

Zhang J Y, Ding T D, Zhang C L. 2013. Biosorption andtoxicity responses to arsenite (As [III]) in Scenedesmusquadricauda. Chemosphere, 92, 1077-1084

Zhao F J, Zhu Y G, Meharg A A. 2013. Methylated arsenicspecies in rice: Geographical variation, origin, and uptakemechanisms. Environmental Science & Technology, 47,3957-3966

Zheng R L, Sun G X, Zhu Y G. 2013. Effects of microbialprocesses on the fate of arsenic in paddy soil. ChineseScience Bulletin, 58, 186-193
[1] Xiaotong Liu, Siwei Liang, Yijia Tian, Xiao Wang, Wenju Liang, Xiaoke Zhang. Effect of land use on soil nematode community composition and co-occurrence network relationship[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2807-2819.
[2] Xianglin Zhang, Jie Xue, Songchao Chen, Zhiqing Zhuo, Zheng Wang, Xueyao Chen, Yi Xiao, Zhou Shi. Improving model performance in mapping cropland soil organic matter using time-series remote sensing data[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2820-2841.
[3] Sainan Geng, Lantao Li, Yuhong Miao, Yinjie Zhang, Xiaona Yu, Duo Zhang, Qirui Yang, Xiao Zhang, Yilun Wang. Nitrogen rhizodeposition from corn and soybean, and its contribution to the subsequent wheat crops[J]. >Journal of Integrative Agriculture, 2024, 23(7): 2446-2457.
[4] Wenjie Yang, Jie Yu, Yanhang Li, Bingli Jia, Longgang Jiang, Aijing Yuan, Yue Ma, Ming Huang, Hanbing Cao, Jinshan Liu, Weihong Qiu, Zhaohui Wang. Optimized NPK fertilizer recommendations based on topsoil available nutrient criteria for wheat in drylands of China[J]. >Journal of Integrative Agriculture, 2024, 23(7): 2421-2433.
[5] Guilong Li, Xiaofen Chen, Wenjing Qin, Jingrui Chen, Ke Leng, Luyuan Sun, Ming Liu, Meng Wu, Jianbo Fan, Changxu Xu, Jia Liu.

Characteristics of the microbial communities regulate soil multi-functionality under different cover crop amendments in Ultisol [J]. >Journal of Integrative Agriculture, 2024, 23(6): 2099-2111.

[6] Shanshan Cai, Lei Sun, Wei Wang, Yan Li, Jianli Ding, Liang Jin, Yumei Li , Jiuming Zhang, Jingkuan Wang, Dan Wei.

Straw mulching alters the composition and loss of dissolved organic matter in farmland surface runoff by inhibiting the fragmentation of soil small macroaggregates [J]. >Journal of Integrative Agriculture, 2024, 23(5): 1703-1717.

[7] Jialin Yang, Liangqi Ren, Nanhai Zhang, Enke Liu, Shikun Sun, Xiaolong Ren, Zhikuan Jia, Ting Wei, Peng Zhang.

Can soil organic carbon sequestration and the carbon management index be improved by changing the film mulching methods in the semiarid region? [J]. >Journal of Integrative Agriculture, 2024, 23(5): 1541-1556.

[8] Jie Song, Dongsheng Yu, Siwei Wang, Yanhe Zhao, Xin Wang, Lixia Ma, Jiangang Li. Mapping soil organic matter in cultivated land based on multi-year composite images on monthly time scales[J]. >Journal of Integrative Agriculture, 2024, 23(4): 1393-1408.
[9] Junyu Xie, Jianyong Gao, Hanbing Cao, Jiahui Li, Xiang Wang, Jie Zhang, Huisheng Meng, Jianping Hong, Tingliang Li, Minggang Xu. Calcium carbonate promotes the formation and stability of soil macroaggregates in mining areas of China[J]. >Journal of Integrative Agriculture, 2024, 23(3): 1034-1047.
[10] Weina Zhang, Zhigan Zhao, Di He, Junhe Liu, Haigang Li, Enli Wang.

Combining field data and modeling to better understand maize growth response to phosphorus (P) fertilizer application and soil P dynamics in calcareous soils [J]. >Journal of Integrative Agriculture, 2024, 23(3): 1006-1021.

[11] Minghui Cao, Yan Duan, Minghao Li, Caiguo Tang, Wenjie Kan, Jiangye Li, Huilan Zhang, Wenling Zhong, Lifang Wu.

Manure substitution improves maize yield by promoting soil fertility and mediating the microbial community in lime concretion black soil [J]. >Journal of Integrative Agriculture, 2024, 23(2): 698-710.

[12] Changqin Yang, Xiaojing Wang, Jianan Li, Guowei Zhang, Hongmei Shu, Wei Hu, Huanyong Han, Ruixian Liu, Zichun Guo.

Straw return increases crop production by improving soil organic carbon sequestration and soil aggregation in a long-term wheat–cotton cropping system [J]. >Journal of Integrative Agriculture, 2024, 23(2): 669-679.

[13] Ilenia Clavero-Camacho, Antonio Archidona-Yuste, Carolina Cantalapiedra-Navarrete, Pablo Castillo, Juan Emilio Palomares-Rius.

Prevalence and ecological factors affecting the distribution of plant-parasitic nematodes in Prunus groves in Spain [J]. >Journal of Integrative Agriculture, 2024, 23(2): 566-589.

[14] Qiuyan Yan, Linjia Wu, Fei Dong, Shuangdui Yan, Feng Li, Yaqin Jia, Jiancheng Zhang, Ruifu Zhang, Xiao Huang.

Subsoil tillage enhances wheat productivity, soil organic carbon and available nutrient status in dryland fields [J]. >Journal of Integrative Agriculture, 2024, 23(1): 251-266.

[15] Tingcheng Zhao, Aibin He, Mohammad Nauman Khan, Qi Yin, Shaokun Song, Lixiao Nie.

Coupling of reduced inorganic fertilizer with plant-based organic fertilizer as a promising fertilizer management strategy for colored rice in tropical regions [J]. >Journal of Integrative Agriculture, 2024, 23(1): 93-107.

No Suggested Reading articles found!