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| Abortive Process of a Novel Rapeseed Cytoplasmic Male Sterility Line Derived from Somatic Hybrids Between Brassica napus and Sinapis alba |
| WANG Juan, GAO Ya-nan, KONG Yue-qin, JIANG Jin-jin, LI Ai-min, ZHANG Yong-tai |
1、Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China
2、Jiangsu Institute of Agricultural Sciences in the Lixiahe District, Yangzhou 225009, P.R.China |
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摘要 Somatic hybridization is performed to obtain significant cytoplasmic male sterility (CMS) lines, whose CMS genes are derived either from the transfer of sterile genes from the mitochondrial genome of donor parent to the counterpart of receptor or production of new sterile genes caused by mitochondrial genome recombination of the biparent during protoplast fusion. In this study, a novel male sterile line, SaNa-1A, was obtained from the somatic hybridization between Brassica napus and Sinapis alba. The normal anther development of the maintainer line, SaNa-1B, and the abortive process of SaNa-1A were described through phenotypic observations and microtome sections. The floral organ of the sterile line SaNa-1A was sterile with a shortened filament and deflated anther. No detectable pollen grains were found on the surface of the sterile anthers. Semi-thin sections indicated that SaNa-1A aborted in the pollen mother cell (PMC) stage when vacuolization of the tapetum and PMCs began. The tapetum radically elongated and became highly vacuolated, occupying the entire locule together with the vacuolated microspores. Therefore, SaNa-1A is different from other CMS lines, such as ogu CMS, pol CMS and nap CMS as shown by the abortive process of the anther.
Abstract Somatic hybridization is performed to obtain significant cytoplasmic male sterility (CMS) lines, whose CMS genes are derived either from the transfer of sterile genes from the mitochondrial genome of donor parent to the counterpart of receptor or production of new sterile genes caused by mitochondrial genome recombination of the biparent during protoplast fusion. In this study, a novel male sterile line, SaNa-1A, was obtained from the somatic hybridization between Brassica napus and Sinapis alba. The normal anther development of the maintainer line, SaNa-1B, and the abortive process of SaNa-1A were described through phenotypic observations and microtome sections. The floral organ of the sterile line SaNa-1A was sterile with a shortened filament and deflated anther. No detectable pollen grains were found on the surface of the sterile anthers. Semi-thin sections indicated that SaNa-1A aborted in the pollen mother cell (PMC) stage when vacuolization of the tapetum and PMCs began. The tapetum radically elongated and became highly vacuolated, occupying the entire locule together with the vacuolated microspores. Therefore, SaNa-1A is different from other CMS lines, such as ogu CMS, pol CMS and nap CMS as shown by the abortive process of the anther.
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Received: 17 February 2013
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31330057), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Program of International S&T Cooperation of China (1021) and the Jiangsu Province Graduate Innovation Fund (XCLX13_899), China. |
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Corresponding Authors:
WANG You-ping, Tel: +86-514-87997303, Fax: +86-514-87991747, E-mail: wangyp@yzu.edu.cn
E-mail: wangyp@yzu.edu.cn
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| About author: WANG Juan |
Cite this article:
WANG Juan, GAO Ya-nan, KONG Yue-qin, JIANG Jin-jin, LI Ai-min, ZHANG Yong-tai.
2014.
Abortive Process of a Novel Rapeseed Cytoplasmic Male Sterility Line Derived from Somatic Hybrids Between Brassica napus and Sinapis alba. Journal of Integrative Agriculture, 13(4): 741-748.
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| Bang S, Tsutsui K K, Shim S, Kaneko Y. 2011. Production and characterization of the novel CMS line of radish. Raphanus sativus. carrying Brassica maurorum cytoplasm. Plant Breeding, 130, 410-412 Bartkowiak-Broda I, Rousellle P, Renard M. 1977. Investigations of two kinds of cytoplasmic male sterility in rapeseed (Brassica napus L.). Genetics Polish, 20, 487-497 Dawson J, Wilson Z A, Aarts M G M, Braithwaite A F, Briarty L G, Mulligan B J. 1993. Microspore and pollen development in six male-sterile mutants of Arabidopsis thaliana. Candian Journal of Botany, 71, 629-638 Fu T D. 1981. Production and research of rapeseed in the People’s Republic of China. Eucarpia Cruciferae Newsletter, 6, 6-7 Fu T D, Yang G S. 1995. Cytoplasmic male sterility in rapeseed. In: Fu T D, ed., Breeding and Utilization of Rapeseed Hybrid. Hubei Science and Technology Press, China. pp. 1-6 Gonzalez-Melendi P, Uyttewaal M, Morcillo C N, Mora J R H, Fajardo S, Budar F, Lucas M M. 2008. A light and electron microscopy analysis of the events leading to male sterility in Ogu-INRA CMS of rapeseed (Brassica napus). Journal Experimental Botany, 59, 827-838 Hao J Y, Li Y C, Hu Q, Mei D S, Li Y D, Xu Y S. 2011. Putative fragment cloning of Nsa CMS restoration gene in Brassica napus. Chinese Journal Oil Crop Science, 33, 433-437 (in Chinese) Havey M J. 2004. The use of cytoplasmic male sterility for hybrid seed production. In: Daniell H, Chase C. eds., Molecular Biology and Biotechnology of Plant Organelles. Springer Verlag, Berlin. pp. 623-634 Holford P, Croft J, Newbury H J. 1991. Structural studies of microsporogenesis in fertile and male-sterile onions. Allium cepa L. containing the cms-S cytoplasm. Theoretical and Applied Genetics, 82, 745-755 Hu Q, Li Y C, Mei D S, Fang X P, Hansen L N, Andersen S B. 2004. Establishment and identification of cyto-plasmic male sterility in Brassica napus L. by intergeneric somatic hybridization. Scientia Agricultura Sinica, 37, 333-338 (in Chinese) Lei S, Yao X, Yi B, Chen W, Ma C, Tu J, Fu T. 2007. Towards map-based cloning: fine mapping of a recessive genic male-sterile gene. BnMs2. in Brassica napus L. and syntenic region identification based on the Arabidopsis thaliana genome sequences. Theoretical and Applied Genetics, 15, 643-651Li A M, Jiang J J, Zhang Y T, Snowdon R J, Liang G H, Wang Y P. 2012. Molecular and cytological characterization of introgression lines in yellow seed derived from somatic hybrids between Brassica napus and Sinapis alba. Molecular Breeding, 29, 209-219Li A M, Wei C X, Jiang J J, Zhang Y T, Snowdon R J, Wang Y P. 2009. Phenotypic variation in the progenies of somatic hybrids between Brassica napus and Sinapis alba. Euphytica, 170, 289-296Liu J H, Landgren M, Glimelius K. 1996. Transfer of the Brassica tournefortii cytoplasm to B. napus for the production of cytoplasmic male sterile B. napus. Physiologia Plantarium, 96, 123-129Liu J H, Xu X Y, Deng X X. 2005. Intergeneric somatic hybridization and its application to crop genetic improvement. Plant Cell, Tissue and Organ Culture, 82, 19-44Liu Z, Liu P, Long F, Hong D, He Q, Yang G. 2012. Fine mapping and candidate gene analysis of the nuclear restorer gene Rfp for pol CMS in rapeseed (Brassica napus L.). Theoretical and Applied Genetics, 125, 773- 779. Mei J, Fu Y, Qian L, Xu X, Li J, Qian W. 2011. Effective widening the gene pool of oilseed rape (Brassica napus L.) by using Chinese B. rapa in a ‘virtual allopolyploid’ approach. Plant Breeding, 130, 333-337Ogura H. 1968. Studies on the new male sterility in Japanese radish, with special references to utilization of this sterility towards the practical raising of hybrid seeds. Memoirs Faculty of Agricultural Kagoshima University, 6, 39-78Pahwa R S, Banga S K, Gogna K P S, Banga S S. 2004. Tournefortii male sterility system in Brassica napus, identification, expression and genetic characterization of male fertility restorers. Plant Breeding, 123, 444-448Pelletier G, Primard C, Vedel F, Chetrit P, Rousselle R R, Renard M. 1983. Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion. Molecular Genomics and Genetics, 191, 244-250Prakash S, Bhat S R, Quiros C F, Kirti P B, Chopra V L. 2009. Brassica and its close allies: Cytogenetics and Evolution. In: Janick J, ed., Plant Breeding Reviews, John Wiley & Sons, UK. pp. 21-187Stiewe G, Roebbelen G. 1994. Establishing cytoplasmic male sterility in Brassica napus by mitochondrial recombination with B. tournefortii. Plant Breeding, 113, 294-304Thompson K F. 1972. Cytoplasmic male sterility in oilseed rape. Heredity, 29, 253-257Uyttewaal M, Arnal N, Quadrado M, Martin-Canadell A, Vrielynck N, Hiard S, Gherbi H, Bendahmane A, Budar F, Mireau H. 2008. Characterization of Raphanus sativus pentatricopeptide repeat proteins encoded by the fertility restorer locus for ogura cytoplasmic male sterility. Plant Cell, 20, 3331-3345Vizcay-Barrena G, Willson Z A. 2006. Altered tapetal PCD and pollen wall development in the Arabidopsis ms1 mutant. Journal of Experimental Botany, 57, 2709-2717Wang J, Jiang J J, Wang Y P. 2013. Protoplast fusion for crop improvement and breeding in China. Plant Cell, Tissue and Organ Culture, 112, 131-142Wang Y P, Sonntag K, Rudloff E, Chen J M. 2005. Intergeric somatic hybridization between Brassica napus L. and Sinapis alba L. Journal of Integrative Plant Biology, 47, 81-91Wei W L, Wang H Z, Liu G H. 2005. Anatomical observation of anther development of NCa, a cytoplasmic male sterile line in rapeseed (Brassica napus L.). Scientia Agricultura Sinica, 38, 1232-1237. (in Chinese) Wei W L, Wang H Z, Liu G H. 2009. Cytological and molecular characterization of a new cytoplasmic male sterility in rapeseed. Plant Breeding, 128, 426-428Wilson Z A, Morroll S M, Dawson J, Swarup R, Tighe P J. 2001. The Arabidopsis MALE STERILITY1 (MS1) gene is a transcriptional regulator of male gametogenesis, with homology to the PHD-finger family of transcription factors. The Plant Journal, 28, 27-39Yi B, Chen Y, Lei S, Tu J, Fu T. 2006. Fine mapping of the recessive genic male-sterile gene (Bnmsl) in Brassica napus L. Theoretical and Applied Genetics, 13, 643-650Yi B, Zeng F, Lei S, Chen Y, Yao X, Zhu Y, Shen J, Ma C, Tu J, Fu T. 2010. Two duplicate CYP704B1- homologous genes BnMs1 and BnMs2 are required for pollen exine formation and tapetal development in Brassica napus. The Plant Journal, 63, 925-938Yuan M, Yang G S, Fu T D, Li Y. 2003. Transcriptional control of orf224/atp6 by the pol CMS restorer Rf gene in Brassica napus L. Acta Genetics Sinica, 30, 469-473. |
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