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Journal of Integrative Agriculture  2017, Vol. 16 Issue (12): 2698-2708    DOI: 10.1016/S2095-3119(17)61733-7
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Cytogenetics and germplasm enrichment in Brassica allopolyploids in China
LI Zai-yun1, WANG You-ping2, 3
1 National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R.China
2 College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, P.R.China
3 Key Laboratory of  Agrobiology of Jiangsu, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.China
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Abstract  This paper reviews research advances in cytogenetics and germplasm innovation in Brassica allopolyploids, particularly oilseed rape (Brassica napus), in China.  Three naturally evolved Brassica allotetraploid species are cytologically stable but tend to preferentially lose several chromosomes from one subgenome when induced by alien chromosome elimination.  A-subgenome is extracted from B. napus, and the ancestral Brassica rapa was restituted after the total loss of C-subgenome chromosomes.  Genome-wide genetic and epigenetic alterations were observed in both natural and synthetic Brassica allotetraploids.  B. napus was subjected to extensive interspecific hybridization with landraces of B. rapa and Brassica juncea, which exhibit abundant phenotype variations, to widen the genetic diversity in breeding and select numerous elite germplasm resources and cultivars; these cultivars include the representative Zhongyou 821, which also parented numerous other varieties.  Novel B. napus genotypes were obtained using Brassica trigenomic hybrids and allohexaploids (2n=54, AABBCC) by combining subgenomes from extant allotetraploids and diploids as bridge.  Alien additions, substitutions, and translocations of the B. napus genome were developed by intergeneric/intertribal sexual and somatic hybridizations with several crucifers.  Furthermore, mitochondrial DNA recombination promoted the production of novel cytoplasmic male sterile lines.  
Keywords:  Brassica napus       germplasm       Brassica rapa       Brassica juncea       cytogenetics  
Received: 08 December 2016   Accepted:
Fund: 

This study was supported by the National Key Research and Development Program of China (2016YFD0102000, 2016YFD0101000), the National Natural Science Foundation of China (31330057), and Yangzhou University for Excellent Talent Support Program, China.

Corresponding Authors:  Correspondence WANG You-ping, Tel: +86-514-87997303, Fax: +86-514-97991747, E-mail: wangyp@yzu.edu.cn   

Cite this article: 

LI Zai-yun, WANG You-ping. 2017. Cytogenetics and germplasm enrichment in Brassica allopolyploids in China. Journal of Integrative Agriculture, 16(12): 2698-2708.

Albertin W, Balliau T, Brabant P, Chevre A M, Eber F, Malosse C, Thiellement H. 2006. Numerous and rapid nonstochastic modifications of gene products in newly synthesized Brassica napus allotetraploids. Genetics, 173, 1101–1113.

Allender C J, King G J. 2010. Origins of the amphiploid species Brassica napus L. investigated by chloroplast and nuclear molecular markers. BMC Plant Biology, 10, 54.

Birchler J A, Veitia R A. 2007. The gene balance hypothesis: From classical genetics to modern genomics. The Plant Cell, 19, 395–402.

Brenchley R, Spannagl M, Pfeifer M, Barker G L, D’Amore R, Allen A M, McKenzie N, Kramer M, Kerhornou A, Bolser D, Kay S, Waite D, Trick M, Bancroft I, Gu Y, Huo N, Luo M C, Sehgal S, Gill B, Kianian S, et al. 2012. Analysis of the bread wheat genome using whole-genome shotgun sequencing. Nature, 491, 705–710.

Chatterjee D, Banga S, Gupta M, Bharti S, Salisbury P A, Banga S S. 2016. Resynthesis of Brassica napus through hybridization between B. juncea and B. carinata. Theoretical and Applied Genetics, 129, 977–990.

Chen H F, Wang H, Li Z Y. 2007. Production and genetic analysis of partial hybrids in intertribal crosses between Brassica species (B. rapa, B. napus) and Capsella bursa-pastoris. Plant Cell Reports, 26,1791–1800.

Chen S, Nelson M N, Chèvre A M, Jenczewski E, Li Z Y, Mason A S, Meng J L, Plummer J A, Pradhan A, Siddique K H M, Snowdon R J, Yan G J, Zhou W J, Cowling WA. 2011. Trigenomic bridges for Brassica improvement. Critical Reviews in Plant Sciences, 30, 524–547.

Chen X, Li M T, Shi J Q, Fu D H, Qian W, Zou J, Zhang C Y, Meng JL. 2008. Gene expression profiles associated with intersubgenomic heterosis in Brassica napus. Theoretical and Applied Genetics, 117,1031–1040.

Chen Z J, Pikaard C S. 1997. Transcriptional analysis of nucleolar dominance in polyploid plants: Biased expression silencing of progenitor rRNA genes is developmentally regulated in Brassica. Proceedings of the National Academy of Sciences of the United States of America, 94, 3442–3447.

Cheng J H, Li Y C, Hu Q, Mei D S, Li Y D, Xu Y S, Wang W M. 2008. Molecular identification and distinctness of Nsa male sterile cytoplasm in Brassica napus. Acta Agronomica Sinica, 34,1946–1952. (in Chinese)

Cui C, Ge X H, Gautam M, Kang L, Li Z Y. 2012. Cytoplasmic and genomic effects on meiotic pairing in Brassica hybrids and allotetraploids from pair crosses of three cultivated diploids. Genetics, 191, 725–738.

Cui C, Ge X H, Zhou Y Y, Li M T, Li Z Y. 2013. Cytoplasmic and genomic effects on non-meiosis-driven genetic changes in Brassica hybrids and allotetraploids from pairwise crosses of three cultivated diploids. PLOS ONE, 8, e65078.

Ding L, Zhao Z G, Ge X H, Li Z Y. 2014. Different timing and spatial separation of parental chromosomes in intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus. Genetics and Molecular Research, 13, 2611–2618.

Du K, Liu Q, Wu X, Jiang J J, Wu J, Fang Y, Li A M, Wang Y P. 2016. Morphological structure and transcriptome comparison of the cytoplasmic male sterility line in Brassica napus (SaNa-1a) derived from somatic hybridization and its maintainer line SaNa-1b. Frontiers in Plant Sciences, 7, 1313.

Du X Z, Ge X H, Zhao Z G, Li Z Y. 2008. Chromosome elimination and fragment introgression and recombination producing intertribal partial hybrids from Brassica napus×Lesquerella fendleri crosses. Plant Cell Reports, 27, 261–271.

Fu D, Qian W, Zou J, Meng J. 2012. Genetic dissection of intersubgenomic heterosis in Brassica napus carrying genomic components of B. rapa. Euphytica, 184, 151–164.

Fu T D. 1981. Production and research of rapeseed in the People’s Republic of China. Eucarpia Cruciferae Newsletter, 6, 6–7.

Gaeta R T, Pires J C, Iniguez-Luy F, Leon E, Osborn T C. 2007. Genomic changes in resynthesized Brassica napus and their effect on gene expression and phenotype. The Plant Cell, 19, 3403–3417.

Ge X H, Ding L, Li Z Y. 2013. Nucleolar dominance and different genome behaviors in hybrids and allopolyploids. Plant Cell Reports, 32, 1661–1673.

Gupta M, Atri C, Agarwal N, Banga S S. 2016. Development and molecular?genetic characterization of a stable Brassica allohexaploid. Theoretical and Applied Genetics, 129, 2085–2100.

Gupta M, Gupta S, Kumar H, Kumar N, Banga S S. 2015. Population structure and breeding value of a new type of Brassica juncea created by combining A and B genomes from related allotetraploids. Theoretical and Applied Genetics, 128, 221–234.

Hosoda T. 1950. On new types of Brassica napus obtained from artificial amphidiploids. I. A new type as a forage crop. Researches of Plant Breeding, 4, 91–95.

Hu Q, Andersen S B, Dixelius C, Hansen L N. 2002. Production of fertile intergeneric somatic hybrids between Brassica napus and Sinapis arvensis for the enrichment of the rapeseed gene pool. Plant Cell Reports, 21,147–152.

Hu Q, Li Y C, Mei D S, Fang X P, Hansen L N, Andorsen S B. 2004. Establishment and identification of cytoplasmic male sterility in Brassica napus by intergeneric somatic hybridization. Scientia Agricultura Sinica, 37, 333–338. (in Chinese)

Hua Y W, Li Z Y. 2006. Genomic in situ hybridization analysis of intergeneric hybrids between Brassica napus and Orychophragmus violaceus and production of B. napus aneuploids. Plant Breeding, 125, 144–149.

Jiang J, Shao Y, Du K, Ran L, Fang X, Wang Y. 2013. Use of digital gene expression to discriminate gene expression differences in early generations of resynthesized Brassica napus and its diploid progenitors. BMC Genomics, 14, 72.

Jiang J, Wang Y, Zhu B, Fang T, Fang Y, Wang Y. 2015. Digital gene expression analysis of gene expression differences within Brassica diploids and allopolyploids. BMC Plant Biology, 15, 22.

Kang L, Du X, Zhou Y, Zhu B, Ge X, Li Z. 2014. Development of a complete set of monosomic alien addition lines between Brassica napus and Isatis indigotica (Chinese woad). Plant Cell Reports, 33,1355–1364.

Lee S S, Lee S A, Yang J, Kim J. 2011. Developing stable progenies of ×Brassicoraphanus, an intergeneric allopolyploid between Brassica rapa and Raphanus sativus, through induced mutation using microspore culture. Theoretical and Applied Genetics, 122, 885–891.

Li A L, Liu D C, Wu J, Zhao X B, Hao M, Geng S, Yan J, Jiang X, Zhang L, Wu J, Yin L, Zhang R, Wu L, Zheng Y, Mao L. 2014. mRNA and small RNA transcriptomes reveal insights into dynamic homoeolog regulation of allopolyploid heterosis in nascent hexaploidy wheat. The Plant Cell, 26, 1878–1900.

Li M T, Chen X, Meng J L. 2006. Intersubgenomic heterosis in rapeseed production with a partial new typed Brassica napus containing subgenome Ar from B. rapa and Cc from Brassica carinata. Crop Science, 46, 234–242.

Li M T, Li Z, Zhang C, Qian W, Meng J L. 2005. Reproduction and cytogenetic characterization of interspecific hybrids derived from crosses between Brassica carinata and

B. rapa. Theoretical and Applied Genetics, 110, 1284–1289.

Li M T, Qian W, Meng J L, Li Z Y. 2004. Construction of novel Brassica napus genotypes through chromosomal substitution and elimination using interploid species hybridization. Chromosome Research, 12, 417–426.

Li Q, Mei J, Zhang Y, Li J N, Ge X H, Li Z Y, Qian W. 2013. A large-scale introgression of genomic components of Brassica rapa into B. napus by the bridge of hexaploid derived from hybridization between B. napus and

B. oleracea. Theoretical and Applied Genetics, 126, 2073–2080.

Li Q F, Zhou Q H, Mei J Q, Zhang Y J, Li J N, Li Z Y, Ge X H, Xiong Z Y, Huang Y J, Qian W. 2014. Improvement of Brassica napus via interspeci?c hybridization between

B. napus and B. oleracea. Molecular Breeding, 34,1955–1963.

Li Z Y, Liu H L, Luo P. 1995. Production and cytogenetics of intergeneric hybrids between Brassica napus and Orychophragmus violaceus. Theoretical and Applied Genetics, 91, 131–136.

Liu H L. 2000. Genetics and Breeding of Rapeseed. China Agricultural University Press, Beijing. (in Chinese).

Liu R, Qian W, Meng J. 2002. Association of RFLP markers and biomass heterosis in trigenomic hybrids of oilseed rape (Brassica napus×B. campestris). Theoretical and Applied Genetics, 105, 1050–1057.

Liu S, Liu Y, Yang X, Tong C, Edwards D, Paterson A H. 2014. The Brassica oleracea genome reveals the asymmetrical evolution of polyploid genomes. Nature Communication, 5, 3930.

Liu Z S, Guan C Y, Chen S Y, Liu S, Yang L. 2010. Transfer of superior traits from Brassica juncea into Brassica napus. Agricultural Science & Technology, 11, 49-52.

Lysak M A, Cheung K, Kitschke M, Bures P. 2007. Ancestral chromosomal blocks are triplicated in Brassiceae species with varying chromosome number and genome size. Plant Physiology, 145, 402–410.

Ma N, Li Z Y. 2007. Development of novel Brassica napus lines with canola quality and higher levels of oleic and linoleic acids derived from intergeneric hybrids between B. napus and Orychophragmus violaceus. Euphytica, 157, 231–238.

Ma N, Li Z Y, Cartagena J A, Fukui K. 2006. GISH and AFLP analyses of novel Brassica napus lines derived from one hybrid between B. napus and Orychophragmus violaceus. Plant Cell Reports, 25, 1089–1093.

Mason A S, Nelson M N, Takahira J, Cowling W A, Alves G M, Chaudhuri A, Chen N, Ragu M E, Dalton-Morgan J, Coriton O, Huteau V, Chevre A M, Batley J. 2014. The fate of chromosomes and alleles in an allohexaploid Brassica population. Genetics, 197, 273–283.

Mason A S, Yan G, Cowling W A, Nelson M N. 2012. A new method for producing allohexaploid Brassica through unreduced gametes. Euphytica, 186, 277–287.

Mathias R. 1985. Transfer of cytoplasmic male sterility from brown mustard (Brassica juncea (L.) Coss.) into rapeseed (Brassica napus L.). Zeitung Pflanzenzüchtg, 95, 371–374.

Mei D S, Li Y C, Hu Q. 2003. Investigation of male sterile lines derived from intergeneric somatic hybrids of Brassica napus(+) Orychophragmus violaceus and B. napus(+) Sinapis arvensis. Chinese Journal of Oil Crop Sciences, 25, 72–75. (in Chinese)

Mei J Q, Ding Y J, Lu K, Wei D, Liu Y, Disi J O, Li J, Liu L, Liu S, Mckay J, Qian W. 2013. Identification of genomic regions involved in resistance against Sclerotinia sclerotiorum from wild Brassica oleracea. Theoretical and Applied Genetics, 126, 549–556.

Meng J, Shi S, Gan L, Li Z, Qu X. 1998. The production of yellow-seeded Brassica napus (AACC) through crossing interspecific hybrids of B. campestris (AA) and B. carinata (BBCC) with B. napus. Euphytica, 103, 329–333.

Nagaharu U. 1935. Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Japanese Journal of Botany, 7, 389–452.

Ogura H. 1968. Studies on the new male-sterility in Japanese radish with special reference to the utilization of this sterility towards the practical raising of hybrid seeds. Memoirs of the Faculty of Agriculture, Kagoshima University, 6, 39–78.

Paterson A H, Wendel J F, Gundlach H, Guo H, Jenkins J, Schmutz J. 2012. Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres. Nature, 492, 423–427.

Pradhan A, Plummer J A, Nelson M N, Cowling W A, Yan G. 2010. Successful induction of trigenomic hexaploid Brassica from a triploid hybrid of B. napus L. and B. nigra (L.) Koch. Euphytica, 176, 87–98.

Prakash S, Bhat S R, Quiros C F, Kirti P B, Chopra V L. 2009. Brassica and its close allies: Cytogenetics and evolution. Plant Breeding Review, 31, 21–187.

Prakash S, Wu X M, Bhat S R. 2011. Brassica and its close allies: Cytogenetics and evolution. Plant Breeding Reviews, 35, 19–84.

Qian W, Chen X, Fu D, Zou J, Meng J. 2005. Intersubgenomic heterosis in seed yield potential observed in a new type of Brassica napus introgressed with partial Brassica rapa genome. Theoretical and Applied Genetics, 110, 1187–1194.

Qian W, Liu R, Meng J. 2003. Genetic effects on biomass yield in interspecific hybrids between Brassica napus and B. rapa. Euphytica, 134, 9–15.

Qian W, Meng J, Li M, Frauen M, Sass O, Noack J, Jung C. 2006. Introgression of genomic components from Chinese Brassica rapa contributes to widening the genetic diversity in rapeseed (B. napus L.), with emphasis on the evolution of Chinese rapeseed. Theoretical and Applied Genetics, 113, 49–54.

Ran L P, Fang T T, Rong H, Jiang J J, Fang Y J, Wang Y P. 2016. Analysis of cytosine methylation in early generations of resynthesized Brassica napus. Journal of Integrative Agriculture, 15, 1228–1238.

Shiga T, Baba S. 1971. Cytoplasmic male sterility in rape plants (Brassica napus L.). Japanese Journal of Breeding, 21, 16–17.

Shinohara S, Kanno M. 1961. “Hakuran” interspecific hybrid between common cabbage and Chinese cabbage. Agriculture and Horticulture, 36, 1189–1190.

Takeda M. 1986. Studies on the breeding of artificially synthesized Brassica napus “Hakuran” with head formation habit and the estabilishment of cropping systems of the F1 hybrids. Research Bulletin of the Faculty College of Agriculture Gifu University, 1, 1–185.

Tan C, Pan Q, Cui C, Xiang Y, Ge X, Li Z. 2016. Genome-wide gene/genome dosage imbalance regulates gene expressions in synthetic Brassica napus and derivatives (AC, AAC, CCA, CCAA). Frontiers in Plant Sciences, 7, 1432.

Tang Z L, Li J N, Zhang X K, Chen L, Wang R. 1997. Genetic variation of yellow-seeded rapeseed lines (Brassica

napus L.) from different genetic sources. Plant Breeding, 116, 471–474.

Tu Y Q, Sun J, Liu Y, Ge X H, Zhao Z G, Yao X C, Li Z Y. 2010. Production and genetic analysis of partial hybrids from intertribal sexual crosses between Brassica napus and Isatis indigotica and progenies. Genome, 53, 146–156.

Vaughan D A, Balázs E, Heslop-Harrison J S. 2007. From crop domestication to super-domestication. Annals of Botany, 100, 893–901.

Wan Z J, Jing B, Tu J X, Ma C Z, Shen J X, Yi B, Wen J, Huang T, Wang X J, Fu T D. 2008. Genetic characterization of a new cytoplasmic male sterility system (hau) in Brassica juncea and its transfer to B. napus. Theoretical and Applied Genetics, 116, 355–362.

Wang J, Gao Y N, Kong Y Q, Jiang J J, Li A M, Zhang Y T, Wang Y P. 2014. Abortive process of a novel rapeseed cytoplasmic male sterility line derived from somatic hybrids between Brassica napus and Sinapis alba. Journal of Integrative Agriculture, 13, 741–748.

Wang J, Jiang J J, Wang Y P. 2013. Protoplast fusion for crop improvement and breeding in China. Plant Cell, Tissue and Organ Culture, 112, 131–142.

Wang W M. 2008. Molecular identification of Nsa cytoplasm in Brassica napus L. MSc thesis, Chinese Academy of Agricultural Sciences, China. (in Chinese)

Wang X, Chen F, Li Y, Du Y, Liao Y, Yin Y. 2011. The genome of the mesopolyploid crop species Brassica rapa. Nature Genetics, 43, 1035–1039.

Wang Y P, Sonntag K, Rudloff E. 2003. Development of rapeseed with high erucic acid content by asymmetric somatic hybridization between Brassica napus and Crambe abyssinica. Theoretical and Applied Genetics, 106, 1147–1155.

Wang Y P, Sonntag K, Rudloff E, Chen J M. 2005a. Intergeneric somatic hybridization between Brassica napus L. and Sinapis alba L. Journal of Integrative Plant Biology, 47, 84–91.

Wang Y P, Sonntag K, Rudloff E, Wehling P, Snowdon R J. 2006. GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition line. Plant Cell Reports, 25, 35–40.

Wang Y P, Zhao X X, Sonntag K, Wehling P, Snowdon R J. 2005b. Behaviour of Sinapis alba chromosomes in a Brassica napus background revealed by genomic in situ hybridization. Chromosome Research, 13, 819–826.

Wen J, Tu J X, Li Z Y, Fu T D, Ma C Z, Shen J X. 2008. Improving ovary and embryo culture techniques for efficient resynthesis of Brassica napus from reciprocal crosses between yellow-seeded diploids B. rapa and B. oleracea. Euphytica, 162, 81–89.

Xiao Y, Chen L, Zou J, Tian E, Xia W, Meng J. 2010. Development of a population for substantial new type Brassica napus diversified at both A/C genomes. Theoretical and Applied Genetics, 121, 1141–1150.

Xu Y, Zhong L, Wu X, Fang X, Wang J. 2009. Rapid alterations of gene expression and cytosine methylation in newly synthesized Brassica napus allopolyploids. Planta, 229, 471–483.

Yang J H, Liu D Y, Wang X W,  Ji C M, Cheng F, Zhang M. 2016. The genome sequence of allopolyploid Brassica juncea and analysis of differential homoeolog gene expression influencing selection. Nature Genetics, 48, 1225–1232.

Zhang D, Pan Q, Tan C, Zhu B, Ge X, Shao Y, Li Z. 2016. Genome-wide gene expressions respond differently to A-subgenome origins in Brassica napus synthetic hybrids and natural allotetraploid. Frontiers in Plant Sciences, 7, 1508.

Zhang D W, Pan Q, Cui C, Tan C, Ge X H, Shao Y, Li Z Y. 2015 Genome-specific differential gene expressions in resynthesized Brassica allotetraploids from pair-wise crosses of three cultivated diploids revealed by RNA-seq. Frontiers in Plant Sciences, 6, 957.

Zhang H K, Zhu B, Qi B, Gou X W, Dong Y Z, Liu B. 2014. Evolution of the BBAA component of bread wheat during its history at the allohexaploid level. The Plant Cell, 26, 2761–2776.

Zhang X, Ge X, Shao Y, Sun G, Li Z. 2013. Genomic change, retrotransposon mobilization and extensive cytosine methylation alteration in Brassica napus introgressions from two intertribal hybridizations. PLOS ONE, 8, e56346. 

Zhao Z G, Hu T T, Ge X H, Du X Z, Ding L, Li Z Y. 2008. Production and characterization of intergeneric somatic hybrids between Brassica napus and Orychophragms violaceus and  their backcrossing progenies. Plant Cell Reports, 27, 1611–1621.

Zhao Z G, Ma N, Li Z Y. 2007. Alteration of chromosome behavior and synchronization of parental chromosomes after successive generations in Brassica napus×Orychophragmus violaceus hybrids. Genome, 50, 226–233.

Zhou J N, Chen T, Cui C, Ge X H, Li Z Y. 2016. Distinct subgenome stabilities in synthesized Brassica allohexaploids. Theoretical and Applied Genetics, 129, 1257–1271.

Zhou R C, Moshgabadi N, Adams K L. 2011 Extensive changes to alternative splicing patterns following allopolyploidy in natural and resynthesized polyploids. Proceedings of the National Academy of Sciences of the United States of America, 108, 16122–16127.

Zhu B, Shao Y, Pan Q, Ge X, Li Z Y. 2015. Genome-wide gene expression perturbation induced by loss of C2 chromosome in allotetraploid Brassica napus L. Frontiers in Plant Sciences, 6, 763.     

Zhu B, Tu Y Q, Zeng P, Ge X H, Li Z Y. 2016. Extraction of the constituent subgenomes of the natural allopolyploid rapeseed (Brassica napus L.). Genetics, 204, 1015–1027.

Zou J, Fu D, Gong H, Qian W, Xia W, Meng J. 2011. De novo genetic variation associated with retrotransposon activation, genomic rearrangements and trait variation in a recombinant inbred line population of Brassica napus derived from interspecific hybridization with Brassica rapa. The Plant Journal, 68, 212–224.

Zou J, Zhu J, Huang S M, Tian E T, Xiao Y, Fu D H, Tu J X, Fu T D, Meng J L. 2010. Broadening the avenue of intersubgenomic heterosis in oilseed Brassica. Theoretical and Applied Genetics, 120, 283–290.
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