|
|
|
|
|
| Chromosome Mapping, Expression and Polymorphism Analysis of CRABP1 Gene in Pigs |
| ZHAO Shuan-ping1, 4, TANG Zhong-lin1, ZHOU Rong1, QU Chang-qing3, ZHENG Jian-wei2 and LI Kui1 |
1、Key Laboratory for Domestic Animal Genetic Resources and Breeding, Ministry of Agriculture/Institute of Animal Science, Chinese Academy
of Agricultural Sciences, Beijing 100193, P.R.China
2、Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R.China
3、School of Life Science, Fuyang Teachers College, Fuyang 236041, P.R.China
4、Institute of Animal Science, Anhui Academy of Agricultural Sciences, Hefei 230031, P.R.China |
|
|
|
摘要 Cellular retinoic acid-binding protein 1 (CRABP1) is a well-conserved member of cytosolic lipid-binding protein family. It is an important modulator of retinoic acid signaling. Long serial analysis of gene expression (LongSAGE) analysis suggested that CRABP1 gene was differentially expressed during prenatal skeletal muscle development in porcine. Here, we obtained the full-length coding region sequence and genomic sequence of the porcine CRABP1 gene and analyzed its genomic structures. Subsequently, we examined CRABP1 chromosome assignment using INRA-University of Minnesota 7 000 porcine radiation hybrid panel (IMpRH) and explored its tissue distribution in adult Tongcheng pigs and dynamical expression profiles in prenatal skeletal muscle (33, 65 and 90 days post coitus, dpc) from Landrace (lean-type) (described as L33, L65 and L90) and Tongcheng pigs (obese-type) (described as T33, T65 and T90). The CRABP1 gene was mapped to chromosome 7q11-q23 and closely linked to the microsatellite marker SWR1928. Quantitative real-time PCR showed that CRABP1 mRNA was highly expressed in lung and stomach, moderately expressed in placenta and uterus, and weakly expressed in other tissues. Moreover, CRABP1 gene was down-regulated during prenatal skeletal muscle development in both Landrace and Tongcheng pigs and it was expressed much higher in T33 than L33. Two single-nucleotide polymorphisms (SNPs) were detected by sequencing and mass spectrometry methods, allele frequency analysis indicated that g. 281 (G>A) and g. 2992 (G>A) were deviated from Hardy-Weinberg equilibrium in the Landrace and DLY (Duroc×(Landrace×Yorkshire)) pig breeds.
Abstract Cellular retinoic acid-binding protein 1 (CRABP1) is a well-conserved member of cytosolic lipid-binding protein family. It is an important modulator of retinoic acid signaling. Long serial analysis of gene expression (LongSAGE) analysis suggested that CRABP1 gene was differentially expressed during prenatal skeletal muscle development in porcine. Here, we obtained the full-length coding region sequence and genomic sequence of the porcine CRABP1 gene and analyzed its genomic structures. Subsequently, we examined CRABP1 chromosome assignment using INRA-University of Minnesota 7 000 porcine radiation hybrid panel (IMpRH) and explored its tissue distribution in adult Tongcheng pigs and dynamical expression profiles in prenatal skeletal muscle (33, 65 and 90 days post coitus, dpc) from Landrace (lean-type) (described as L33, L65 and L90) and Tongcheng pigs (obese-type) (described as T33, T65 and T90). The CRABP1 gene was mapped to chromosome 7q11-q23 and closely linked to the microsatellite marker SWR1928. Quantitative real-time PCR showed that CRABP1 mRNA was highly expressed in lung and stomach, moderately expressed in placenta and uterus, and weakly expressed in other tissues. Moreover, CRABP1 gene was down-regulated during prenatal skeletal muscle development in both Landrace and Tongcheng pigs and it was expressed much higher in T33 than L33. Two single-nucleotide polymorphisms (SNPs) were detected by sequencing and mass spectrometry methods, allele frequency analysis indicated that g. 281 (G>A) and g. 2992 (G>A) were deviated from Hardy-Weinberg equilibrium in the Landrace and DLY (Duroc×(Landrace×Yorkshire)) pig breeds.
|
|
Received: 05 December 2012
Accepted: 06 May 2014
|
| Fund: This work was supported by the National High Technology Research and Development Program of China (2011AA100302), the National Key Technology R&D Program of China (2011ZX08009-001), the National Natural Science Foundation of China (31171192), and the National Basic Research Program of China (2012CB124706). |
|
Corresponding Authors:
ZHENG Jian-wei, E-mail: zjw2001309@hotmail.com
E-mail: zjw2001309@hotmail.com
|
| About author: ZHAO Shuan-ping, E-mail: zhaoshuanping@163.com |
Cite this article:
ZHAO Shuan-ping1, 4 , TANG Zhong-lin1, ZHOU Rong1, QU Chang-qing3, ZHENG Jian-wei2 and LI Kui1.
2014.
Chromosome Mapping, Expression and Polymorphism Analysis of CRABP1 Gene in Pigs. Journal of Integrative Agriculture, 13(5): 1051-1057.
|
| Akama K, Horikoshi T, Nakayama T, Otsu M, Imaizumi N, Nakamura M, Toda T, Inuma M, Hirano H, Kondo Y, Suzuki Y, Inoue N. 2010. Proteomic identification of differentially expressed genes in neural stem cells and neurons differentiated from embryonic stem cells of cynomolgus monkey (Macaca fascicularis) in vitro. Biochimica et Biophysica Acta, 1814, 265-276 Alsop D, Brown S, van der Kraak G. 2001. Development of a retinoic acid receptor-binding assay with rainbow trout tissue: Characterization of retinoic acid binding, receptor tissue distribution, and developmental changes. General and Comparative Endocrinology, 123, 254-267 Banz C, Ungethuem U, Kuban R J, Diedrich K, Lengyel E, Hornung D. 2009. The molecular signature of endometriosis-associated endometrioid ovarian cancer differs significantly from endometriosis-independent endometrioid ovarian cancer. Fertility and Sterility, 94, 1212-1217 Bi J, Hu X, Zhou F C, Wei L N. 2001. Upregulation of cellular retinoic acid-binding protein I expression by ethanol. Development, Growth & Differentiation, 43, 553-561 Chen J, Sun M, Lee S, Zhou G, Rowley J D, Wang S M. 2002. Identifying novel transcripts and novel genes in the human genome by using novel SAGE tags. Proceedings of the National Academy of Sciences of the United States of America, 99, 12257-12262 Collins C A, Watt F M. 2008. Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling. Developmental Biology, 324, 55-67 Conaway H H, Persson E, Halen M, Granholm S, Svensson O, Pettersson U, Lie A, Lerner U H. 2009. Retinoids inhibit differentiation of hematopoietic osteoclast progenitors. The FASEB Journal, 23, 3526-3538 Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P, Frelat G, Gellin J. 1996. Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics, 36, 252- 262. Hawthorn L, Stein L, Varma R, Wiseman S, Loree T, Tan D. 2004. TIMP1 and SERPIN-A overexpression and TFF3 and CRABP1 underexpression as biomarkers for papillary thyroid carcinoma. Head & Neck, 26, 1069- 1083.Lane M A, Xu J, Wilen E W, Sylvester R, Derguini F, Gudas L J. 2008. LIF removal increases CRABPI and CRABPII transcripts in embryonic stem cells cultured in retinol or 4-oxoretinol. Molecular and Cellular Endocrinol, 280, 63-74 Lee Y J, Yu S L, Jung K C, Jung H J, Kim K S, Park C S, Jin D I, Lee J H. 2006. Assignment of cellular retinoic acid-binding protein 1 (CRABP1) and 2 (CRABP2) to porcine chromosome 7q12-->q23 and 4q21-->q23 by somatic cell and radiation hybrid panel mapping. Cytogenetic and Genome Research, 112, 180B. Li H, Su L N, Liu D J, Li X F, Xu R G. 2012. Cloning and expression of cellular retinoic acid binding protein I gene in inner Mongolian cashmere goats. Scientia Agricultura Sinica, 45, 2743-2750 (in Chinese)Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 25, 402-408 Miyake T, Ueda Y, Matsuzaki S, Miyatake T, Yoshino K, Fujita M, Nomura T, Enomoto T, Kimura T. 2010. CRABP1-reduced expression is associated with poorer prognosis in serous and clear cell ovarian adenocarcinoma. Journal of Cancer Research and Clinical Oncology, 137, 715-722 Park S W, Li G, Lin Y P, Barrero M J, Ge K, Roeder R G, Wei L N. 2005. Thyroid hormone-induced juxtaposition of regulatory elements/factors and chromatin remodeling of Crabp1 dependent on MED1/TRAP220. Molecular Cell, 19, 643-653 Poulain S, Evenou F, Carre M C, Corbel S, Vignaud J M, Martinet N. 2009. Vitamin A/retinoids signalling in the human lung. Lung Cancer, 66, 1-7 Pravenec M, Kren V, Wang J, Kurtz T W. 1997. Linkage mapping of the cellular retinoic acid-binding protein 1 (Crabp1) gene to rat chromosome 8. Mammalian Genome, 8, 455-456 Ross S A, McCaffery P J, Drager U C, de Luca L M. 2000. Retinoids in embryonal development. Physiological Reviews, 80, 1021-1054 Stachurska E, Loboda A, Niderla-Bielinska J, Szperl M, Juszynski M, Jozkowicz A, Dulak J, Ratajska A. 2011. Expression of cellular retinoic acid-binding protein I and II (CRABP and II) in embryonic mouse hearts treated with retinoic acid. Acta Biochimica Polonica, 58, 19-29 Park S W, Huang W H, Persaud S D, Wei L N. 2009. RIP140 in thyroid hormone-repression and chromatin remodeling of Crabp1 gene during adipocyte differentiation. Nucleic Acids Research, 37, 7085-7094 Tang Z L, Li Y, Wan P, Li X P, Zhao S H, Liu B, Fan B, Zhu M J, Yu M, Li K. 2007a. LongSAGE analysis of skeletal muscle at three prenatal stages in Tongcheng and Landrace pigs. Genome Biology, 8, R115. Tang Z L, Li Y, Zhao S H, Liu B, Fan B, Li K. 2007b. A modified GLGI method for identification of novel porcine genes from long serial analysis of gene expression tags. Chinese Journal of Agricultural Biotechnology, 4, 111-115 Uhrig M, Brechlin P, Jahn O, Knyazev Y, Weninger A, Busia L, Honarnejad K, Otto M, Hartmann T. 2008. Upregulation of CRABP1 in human neuroblastoma cells overproducing the Alzheimer-typical Abeta42 reduces their differentiation potential. BMC Medicine, 6, 38. Wei L N, Blaner W S, Goodman D S, Nguyen-Huu M C. 1989. Regulation of the cellular retinoid-binding proteins and their messenger ribonucleic acids during P19 embryonal carcinoma cell differentiation induced by retinoic Acid. Molecular Endocrinology, 3, 454-463 Wei L N, Chang L, Hu X. 1999. Studies of the type I cellular retinoic acid-binding protein mutants and their biological activities. Molecular and Cellular Biochemistry, 200, 69-76 Wei L N, Lee C H, Chang L. 1995. Retinoic acid induction of mouse cellular retinoic acid-binding protein-I gene expression is enhanced by sphinganine. Molecular and Cellular Endocrinology, 111, 207-211 Yerle M, Pinton P, Robic A, Alfonso A, Palvadeau Y, Delcros C, Hawken R, Alexander L, Beattie C, Schook L, Millan D, Gellin J. 1998. Construction of a whole- genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenetics and Cell Genetics, 82, 182-188 Zhao S P, Zhao Y Z, Niu P X, Wang N, Tang Z L, Zan L S, Li K. 2011. Molecular characterization of porcine MMP19 and MMP23B genes and its association with immune traits. International Journal of Biological Science, 7, 1101-1113 Zile M H. 2001. Function of vitamin A in vertebrate embryonic development. Journal of Nutrition, 131, 705- 708. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
