|
|
|
|
|
| Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits |
| NIU Bu-yue, LAN Xiao-ming, YAN Xiao-hong, DI Sheng-wei, WANG Yang, LI Hai-tao, XIONG Yuanzhu, WANG Xi-biao |
1.College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, P.R.China
2.Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture/Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education/Huazhong Agricultural University, Wuhan 430070, P.R.China |
|
|
|
摘要 The matrix metalloproteinase 23 (MMP-23), which might play a role in ovulation in mammals, was one of the promising candidate genes for litter size traits in pigs. In the present research, partial sequence of porcine MMP-23 (pMMP-23) gene, including exons 2-8 (GenBank: EU360790), was obtained. Real-time PCR analysis revealed that pMMP-23 gene was highly expressed in ovary. PCR-Sau3A I-RFLP and PCR-Acc II-RFLP assay were established to detect a C/T mutation in exon 3 (EU360790: g. 269C>T) and an A/G mutation in exon 4 (EU360790: g. 505A>G), respectively. Association study for these two SNPs with litter size was assessed in three independent populations (Minzhu, Landrace I and Landrace II). Statistical analysis demonstrated that for second and subsequent litters, TT sows produced more TNB than CC pigs in Landrace breed (P<0.05) at g. 269C>T locus, and the additive effect was significant (P<0.05); GG sows produced more TNB and NBA than AA pigs in Minzhu (P<0.01) and Landrace breeds (P<0.05) at g. 505A>G locus, and the additive effect was significant (P<0.01 or P<0.05). Our study suggested that the pMMP-23 gene might be a novel candidate gene for litter size traits, and g. 505A>G locus might be a useful molecular marker for marker assisted selection (MAS).
Abstract The matrix metalloproteinase 23 (MMP-23), which might play a role in ovulation in mammals, was one of the promising candidate genes for litter size traits in pigs. In the present research, partial sequence of porcine MMP-23 (pMMP-23) gene, including exons 2-8 (GenBank: EU360790), was obtained. Real-time PCR analysis revealed that pMMP-23 gene was highly expressed in ovary. PCR-Sau3A I-RFLP and PCR-Acc II-RFLP assay were established to detect a C/T mutation in exon 3 (EU360790: g. 269C>T) and an A/G mutation in exon 4 (EU360790: g. 505A>G), respectively. Association study for these two SNPs with litter size was assessed in three independent populations (Minzhu, Landrace I and Landrace II). Statistical analysis demonstrated that for second and subsequent litters, TT sows produced more TNB than CC pigs in Landrace breed (P<0.05) at g. 269C>T locus, and the additive effect was significant (P<0.05); GG sows produced more TNB and NBA than AA pigs in Minzhu (P<0.01) and Landrace breeds (P<0.05) at g. 505A>G locus, and the additive effect was significant (P<0.01 or P<0.05). Our study suggested that the pMMP-23 gene might be a novel candidate gene for litter size traits, and g. 505A>G locus might be a useful molecular marker for marker assisted selection (MAS).
|
|
Received: 29 November 2011
Accepted:
|
| Fund: This study was supported by the Educational Commission of Heilongjiang Province, China (11541031). |
|
Corresponding Authors:
Correspondence WANG Xi-biao, Tel: +86-451-55191109, E-mail: wxibiao1967@yahoo.com.cn
|
| About author: NIU Bu-yue, E-mail: niubuyue@163.com |
Cite this article:
NIU Bu-yue, LAN Xiao-ming, YAN Xiao-hong, DI Sheng-wei, WANG Yang, LI Hai-tao, XIONG Yuanzhu, WANG Xi-biao.
2013.
Characterization of Porcine Matrix Metalloproteinase 23 (pMMP-23) Gene and Its Association with Litter Size Traits. Journal of Integrative Agriculture, 12(1): 103-109.
|
| [1]Bennett G L, Leymaster K A. 1989. Integration of ovulationrate, potential embryonic viability and uterine capacityinto a model of litter size in swine. Journal of AnimalScience, 67, 1230-1241[2]Curry Jr T E, Osteen K G. 2003. The matrix metalloproteinasesystem: changes, regulation, and impact throughoutthe ovarian and uterine reproductive cycle. EndocrineReview, 24, 428-465[3]Davidson R K, Waters J G, Kevorkian L, Darrah C, CooperA, Donell S T, Clark I M. 2006. Expression profiling ofmetalloproteinases and their inhibitors in synovium andcartilage. Arthritis Research and Therapy, 8, 124-134[4]Egeblad M, Werb Z. 2002. New functions for the matrixmetalloproteinases in cancer progression. NatureReview Cancer, 2, 161-174[5]Li K, Ren J, Xing Y, Zhang Z, Ma J, Guo Y, Huang L. 2009.Quantitative trait loci for litter size and prenatal loss ina White Duroc×Chinese Erhualian resource population.Animal Genetics, 40, 963-966[6]Liu B H. 1998. Statistical Genomics: Linkage, Mappingand QTL Analysis. CRC Press, LLC, USA.Ohnishi J, Ohnishi E, Jin M, Hirano W, Nakane D, MatsuiH, Kimura A, Sawa H, Nakayama K, Shibuya H, et al.2001. Cloning and characterization of a rat ortholog ofMMP-23 (matrix metalloproteinase-23), a unique typeof membrane-anchored matrix metalloproteinase andconditioned switching of its expression during theovar ian fol l i cular deve lopment MolecularEndocrinology, 15, 747-764[7]Ohnishi J, Ohnishi E, Shibuya H, Takahashi T. 2005.Functions for proteinases in the ovulatory process.Biochimical et Biophysica Acta (Proteins andProteomics), 1751, 95-109[8]Ohnishi J, Ohnishi E, Takahashi T. 2004. The possibleinvolvement of matrix metalloproteinase-23 (MMP-23)activity in ovulatory processes and conditionedswitching of its expression during folliculardevelopment In: Proceedings of 37th Annual Meetingof the Society for the Study of Reproduction. Vancouver,BC, Canada.[9]Onteru S K, Ross J W, Rothschild M F. 2009. The role ofgene discovery, QTL analyses and gene expression inreproductive traits in the pig. Society ReproductionFertility Supplement, 66, 87-102[10]Soller M. 1994. Marker assisted selection - an overview.Animal Biotechnology, 5, 193-207[11]Sun X, Mei S, Tao H, Wang G, Su L, Jiang S, Deng C, XiongY, Li F. 2011. Microarray profiling for differential geneexpression in PMSG-hCG stimulated preovulatoryovarian follicles of Chinese Taihu and LargeWhite sows.BMC Genomics, 12, 111.[12]Velasco G, Pendás A M, Fueyo A, Knäuper V, Murphy G,López-Otín C. 1999. Cloning and characterization ofhuman MMP-23, a new matrix metalloproteinasepredominantly expressed in reproductive tissues andlacking conserved domains in other family members Journal of Biological Chemistry, 274, 4570-4576[13]Wilkie P J, Paszek AA, Beattie C W, Alexander L J,WheelerM B, Schook L B. 1999. A genomic scan of porcinereproductive traits reveals possible quantitative traitloci (QTLs) for number of corpora lutea. MammalianGenome, 10, 573-578[14]Xiong Y Z. 1999. Pig Biochemistry and MolecularGenetics: Experimental Introduction. ChineseAgriculture Press, Beijing. (in Chinese)Yong L D, Leymaster K A, Christenson R K. 1996.Opportunities for indirect selection for uterine capacityof swine. Journal of Animal Science, 74, 119-123 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
