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| Cloning One CIPK Gene from a Thermo-Sensitive Genic Self-Incompatible Line in Maize Expressing Under Different Temperatures |
| LIN Xing-e, JI Hong-qing, NIU Jun-hai, HU Yan-min, FU Zhong-jun, LIU Zong-hua and TANG Ji-hua |
| College of Agronomy, Henan Agricultural University, Zhengzhou 450002, P.R.China |
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摘要 A calcineurin B-like protein-interacting protein kinases (CIPK) gene was cloned (EU 424058) from a thermo-sensitive genic self-incompatibility (TSGI) line, HE97, in maize, which was selected for suppression subtractive hybridization (SSH) library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium (Ca2+). High Ca2+ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility (SI) under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of HE97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.
Abstract A calcineurin B-like protein-interacting protein kinases (CIPK) gene was cloned (EU 424058) from a thermo-sensitive genic self-incompatibility (TSGI) line, HE97, in maize, which was selected for suppression subtractive hybridization (SSH) library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium (Ca2+). High Ca2+ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility (SI) under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of HE97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.
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Received: 10 June 2011
Online: 10 June 2011
Accepted:
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Corresponding Authors:
TANG Ji-hua,
E-mail: Tangjihua1@163.com
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| About author: Correspondence TANG Ji-hua, Professor, Tel: +86-371-63558127, Fax: +86-371-63558122, E-mail: Tangjihua1@163.com |
Cite this article:
LIN Xing-e, JI Hong-qing, NIU Jun-hai, HU Yan-min, FU Zhong-jun, LIU Zong-hua, TANG Ji-hua.
2011.
Cloning One CIPK Gene from a Thermo-Sensitive Genic Self-Incompatible Line in Maize Expressing Under Different Temperatures. Journal of Integrative Agriculture, 10(6): 813-819.
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