|
|
|
|
|
| Cloning One CIPK Gene from a Thermo-Sensitive Genic Self-Incompatible Line in Maize Expressing Under Different Temperatures |
| LIN Xing-e, JI Hong-qing, NIU Jun-hai, HU Yan-min, FU Zhong-jun, LIU Zong-hua and TANG Ji-hua |
| College of Agronomy, Henan Agricultural University, Zhengzhou 450002, P.R.China |
|
|
|
摘要 A calcineurin B-like protein-interacting protein kinases (CIPK) gene was cloned (EU 424058) from a thermo-sensitive genic self-incompatibility (TSGI) line, HE97, in maize, which was selected for suppression subtractive hybridization (SSH) library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium (Ca2+). High Ca2+ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility (SI) under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of HE97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.
Abstract A calcineurin B-like protein-interacting protein kinases (CIPK) gene was cloned (EU 424058) from a thermo-sensitive genic self-incompatibility (TSGI) line, HE97, in maize, which was selected for suppression subtractive hybridization (SSH) library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium (Ca2+). High Ca2+ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility (SI) under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of HE97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.
|
|
Received: 10 June 2011
Accepted:
Online: 10 June 2011
|
|
Corresponding Authors:
TANG Ji-hua,
|
| About author: Correspondence TANG Ji-hua, Professor, Tel: +86-371-63558127, Fax: +86-371-63558122, E-mail: Tangjihua1@163.com |
Cite this article:
LIN Xing-e, JI Hong-qing, NIU Jun-hai, HU Yan-min, FU Zhong-jun, LIU Zong-hua, TANG Ji-hua.
2011.
Cloning One CIPK Gene from a Thermo-Sensitive Genic Self-Incompatible Line in Maize Expressing Under Different Temperatures. Journal of Integrative Agriculture, 10(6): 813-819.
|
| Ansaldi R, Chaboud A, Dumas C. 2000. Multiple S gene family members including natural antisense transcripts are differentially expressed during development of maize flowers.Journal of Biological Chemistry, 31, 24146-24155.Ashburner M, Ball C A, Blake J A, Botstein D, Butler H, Cherry J M, Davis A P, Dolinski K, Dwight S S, Eppig J T, et al.2000. Gene ontology: tool for the unification of biology. Nature Genetics, 25, 25-29.Becraft P W. 1998. Receptor kinases in plant development. Trends in Plant Science, 3, 384-388.Cabrillac D, Cock J M, Dumas C, Gaude T. 2001. The S-locus receptor kinase is inhibited by thioredoxins and activated by pollen coat proteins. Nature, 410, 220-223.Diatchenko L, Lau Y F C, Campbell A P, Chenchik A, Moqadam F, Huang B, Lukyanov K, Gurskaya N, Sverdlov E D, Siebert P D. 1996. Suppression subtractive hybridization: a method of generating differentially regulated or tissue-specific cDNA probes and libraries. Proceedings of the National Academy of Sciences of the USA, 93, 6025-6030.Dodds P N, Ferguson C, Clarke A E, Newbigin E. 1999. Pollenexpressed S-RNases are not involved in self-incompatibility in Lycopersicon peruvianum. Sexual Plant Reproduction, 12,76-87.East E M. 1940. The distribution of self-sterility in flowering plants. Proceedings of the American Philosophical Society, 82, 449-518.Franklin-Tong V E. 1999. Signaling and the modulation of pollen tube growth. The Plant Cell, 11, 727-738.Franklin-Tong V E, Hackett G, Hepler P K. 1997. Ratioimaging of [Ca2+]i in the self-incompatibility response in pollen tubes of Papaver rhoeas. The Plant Journal, 12, 1375-1386.Franklin-Tong V E, Holdaway-Clarke T L, Straatman K R, Kunkel J G, Hepler P K. 2002. Involvement of extracellular calcium influx in the self-incompatibility response of Papaver rhoeas. The Plant Journal, 29, 333-345.Franklin-Tong V E, Ride J P, Franklin F C H. 1995. Recombinant stigmatic self-incompatibility (S-) protein elicits a Ca2+ transient in pollen of Papaver rhoeas. The Plant Journal, 8, 299-307.Franklin-Tong V E, Ride J P, Read N D, Trewavas A J, Franklin FCH. 1993. The self-incompatibility response in Papaver rhoeas is mediated by cytosolic-free calcium. The Plant Journal, 4, 163-177.Geitmann A, Snowman B N, Emons A M, Franklin-Tong V E.2000. Alterations in the actin cytoskeleton of pollen tubes are induced by the self-incompatibility reaction in Papaver rhoeas. The Plant Cell, 12, 1239-1252.Goring D R, Banks P, Fallis L, Baszczynski C L, Beversdorf W D, Rothstein S J. 1992. Identification of an S-locus glycoprotein allele introgressed from Brassica napus ssp.rapifera to Brassica napus ssp. oleifera. The Plant Journal,2, 983-989.Hackauf B, Wehling P. 2005. Approaching the self-incompatibility locus Z in rye (Secale cereale L.) via comparative genetics.Theoretical and Applied Genetics, 110, 832-845.Hanks S K, Quinn A M, Hunter T. 1988. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science, 241, 42-52.Jordan N D, Ride J P, Rudd J J, Davies E M, Vernonica E,Franklin T, Christopher F, Franklin H. 2000. Inhibition of self-incompatible pollen in Papaver rhoeas involves a compels series of cellular events. Annals of Botany, 85, 197-202.Kai N, Suzuki G, Watanabe M, Isogai A, Hinata K. 2001. Sequence comparisons among dispersed members of the Brassica S multi 2 gene family in an S9 genome. Molecular Genetics and Genomics, 265, 526-534.Kendall W A, Taylor N L. 1969. Effect of temperature on pseudoself-compatibility in Trifolium pretense L. Theoretical and Applied Genetics, 39, 123-126.Lan X G, Yu X M, Li Y H. 2005. Progress in study on signal transduction of gametophytic self-incompatibility. Hereditas,27, 677-685. (in Chinese)Lease K, Ingham E, Walker J C. 1998. Challenges in understanding RLK function. Current Opinion in Plant Biology, 1, 388-392.Lin X E, Xie H L, Xi Z Y, Hu Y M, Zhao G Y, Duan L J, Hao Z Y, Liu Z H, Tang J H. 2009. Identification and mapping of a thermo-sensitive genic self-incompatibility gene in maize.Genes & Genomics, 31, 227-234.Malhó R, Trewavas A J. 1996. Localized apical increases of cytosolic free calcium control pollen tube orientation. The Plant Cell, 8, 1935-1949.Marchler-Bauer A, Anderson J B, DeWeese-Scott C, Fedorova N D, Geer L Y, He S, Hurwitz D I, Jackson J D, Jacobs A R,Lanczycki C J, et al. 2003. CDD: a curated Entrez database of conserved domain alignments. Nucleic Acids Research, 31, 383-387.Nasrallah J B, Yu S M, Nasrallah M E. 1988. Self-incompatibility genes of Brassica oleracea: expression, isolation, and structure. Proceedings of the National Academy of Sciences of the USA, 85, 5551-5555.Natalia G E, Chou C F, Lin W W, Hsieh S H, Campbell R D.1998. The G11 gene located in the major histocompatibility complex encodes a novel nuclear serine/threonine protein kinase. Journal of Biological Chemistry, 273, 30954-30960.Nettancourt D D. 2001. Incompatibility and Incongruity in Wild and Cultivated Plants. 2nd ed. Springer-Verlag, Berlin. p. 322.Rudd J J, Franklin-Tong V E. 1999. Calcium signalling in plants. Cellular and Molecular Life Sciences, 55, 214-232.Stein J C, Howlett B, Boyes D C, Nasrallah M E, Nasrallah J B.1991. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proceedings of the National Academy of Sciences of the USA, 88, 8816-8820.Stone J M, Walker J C. 1995. Plant protein kinase families and signal transduction. Plant Physiology, 108, 451-457.Takayama S J, Isogai A. 2003. Molecular mechanism of selfrecognition in Brassica self-incompatibility. Journal of Experimental Botany, 54, 149-156.Townsend C E. 1968. Self-compatibility studies with diploid alsike clover, Trifolium hybridum L. III. Response to Temperature. Crop Science, 8, 269-272.Townsend C E. 1970. Inheritance of a self-compatibility response to temperature and the segregation of S alleles in diploid alsike clover, Trifolium hybridum L. Crop Science, 10, 558-563.Townsend C E, Danielson R E. 1968. Non-translocation of temperature-induced self-compatibility substances(s) in alsike clover, Trifolium hybridum L. Crop Science, 8, 493-495.Walker J C, Zhang R. 1990. Relationship of a putative receptor protein kinase from maize to the S-locus glycoproteins of Brassica. Nature, 345, 743-746.Wilkins P W, Thorogood D. 1992. Breakdown of selfincompatibility in perennial ryegrass at high temperature and its uses in breeding. Euphytica, 64, 65-69.Zhang R, Walker J C. 1993. Structure and expression of the Slocus-related genes of maize. Plant Molecular Biology, 21,1171-1174. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
