Please wait a minute...
Journal of Integrative Agriculture  2012, Vol. 12 Issue (11): 1828-1835    DOI: 10.1016/S1671-2927(00)8717
PREFACE Advanced Online Publication | Current Issue | Archive | Adv Search |
Influence of Diazotrophic Bacteria on Antioxidant Enzymes and Some Biochemical Characteristics of Soybean Subjected to Water Stress
 Hamed Zakikhani, Mohammad RezaArdakani, Farhad Rejali, Majid Gholamhoseini, Aydin Khodaei Joghan
1.Division of Sustainable Agriculture, Agricultural Research Center, Islamic Azad University Karaj Branch, Karaj 31485-313, Iran
2.Soil and Water Research Institute, Karaj 31785-311, Iran
3.Agronomy Department, Faculty of Agriculture, Tarbiat Modares University, Tehran 14115-111, Iran
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  Drought stress is an abiotic stress that imposes serious constraints on plants. The present investigation was carried out to determine the inter-relationship between some physiological attributes of soybeans affected by drought stress and pure isolates of Azotobacter and Azospirillum. Drought stress and bacterial application increased catalase and glutathione peroxidase activity, whereas drought stress increased superoxide dismutase activity during the pod-filling stage. Abscisic acid and proline levels increased due to drought stress and bacterial application during the flowering stage, whereas total plant nitrogen was enhanced under well-watered conditions when plants were inoculated with bacteria. The close relationship between enzyme activity and drought stress with bacteria indicated that antioxidant enzymes play an important role in alleviating the detrimental effects of water stress. In addition, the enhancement of abscisic acid and proline could be positively linked with drought stress, and drought-induced abscisic acid could induce proline accumulation and the expression of antioxidant enzyme genes.

Abstract  Drought stress is an abiotic stress that imposes serious constraints on plants. The present investigation was carried out to determine the inter-relationship between some physiological attributes of soybeans affected by drought stress and pure isolates of Azotobacter and Azospirillum. Drought stress and bacterial application increased catalase and glutathione peroxidase activity, whereas drought stress increased superoxide dismutase activity during the pod-filling stage. Abscisic acid and proline levels increased due to drought stress and bacterial application during the flowering stage, whereas total plant nitrogen was enhanced under well-watered conditions when plants were inoculated with bacteria. The close relationship between enzyme activity and drought stress with bacteria indicated that antioxidant enzymes play an important role in alleviating the detrimental effects of water stress. In addition, the enhancement of abscisic acid and proline could be positively linked with drought stress, and drought-induced abscisic acid could induce proline accumulation and the expression of antioxidant enzyme genes.
Keywords:  abscisic acid       Azospirillum       Azotobacter       proline       soybean       water stress  
Received: 26 October 2011   Accepted:
Corresponding Authors:  Correspondence Aria Dolatabadian, Tel: +98-21-44196522-23, +98-21-44194911-4, Fax: +98-21-44196524, E-mail: aria_dolat2000@yahoo.com     E-mail:  aria_dolat2000@yahoo.com

Cite this article: 

Hamed Zakikhani, Mohammad RezaArdakani, Farhad Rejali, Majid Gholamhoseini, Aydin Khodaei Joghan. 2012. Influence of Diazotrophic Bacteria on Antioxidant Enzymes and Some Biochemical Characteristics of Soybean Subjected to Water Stress. Journal of Integrative Agriculture, 12(11): 1828-1835.

[1]Araus J L, Slafer G A, Reynolds M P, Royo C. 2002. Plantbreeding and drought in C3 cereals: what should webreed for? Annals of Botany, 89, 925-940

[2]Asiri A C M, Cornic G, Jouanin L, Foyer C H. 1998. Overexpression of Fe-SOD IN transformed poplar modifiesthe regulation of photosynthesis at low CO2 partialpressure or following exposure to the pro-oxidantherbicide methyl viologen. Plant Physiology, 117, 565-574

[3]Bates L S, Waldern R P, Teare I K. 1973. Rapid determinationof free proline for water stress studies. Plant Soil, 39,205-207

[4]Breusegem F, van Montagu M, van Inze D, van BreusegemF, van Montagu M. 1998. Engineering stress tolerancein maize. Outlook on Agriculture, 27, 115-124

[5]Brigelius-Flohe R, Flohe L. 2003. Is there a role ofgluta thione peroxidas es in s ignal ing anddifferentiation? Biofactors, 17, 93-102

[6]Caba J M, Lluch C, Ligero F. 1994. Genotypic variability ofnitrogen metabolism enzymes in nodulated roots of viciafaba. Soil Biology and Biochemistry, 27, 785-789

[7]Cakmak I, Horst W J. 1991. Effect of aluminum on lipidperoxidation, superoxide dismutase, catalase, andperoxidase activities in root tips of soybean (Glycinemax). Physiologia Plantarum, 83, 463-468

[8]Cardenas L, Martinez A, Sanchez F, Quinto C. 2008. Fasttransient and specific intracellular ROS changes inliving root hair cells responding to Nod factors (NFs).The Plant Journal, 56, 802-813

[9](in Press)Chen S, Vaghchhipawala Z, Li W, Asard H, Dickman M B.2004. Tomato phospholipids hydro peroxide glutathioneperoxidase plants. Plant Physiology, 135, 1630-1641

[10]Dhanda S, Sethi G S, Behl R K. 2004. Indices of droughttolerance in wheat genotypes at early stages of plantgrowth. Journal of Agronomy and Crop Science, 190,6-12

[11]Fehr W R, Caviness C E. 1977. Stages of SoybeanDevelopment. Special Report 80, Agriculture and HomeEconomics Experiment Station, Lowa State University,IA, USA.Giannopolitis C N, Ries S K. 1977. Superoxide dismutase inhigher plants. Plant Physiology, 59, 309-314

[12]Guan L, Zhao J, Scandalios J G. 2000. Cis-elements andtransfactors that regulate expression of the maize Cat1antioxidant gene in response to ABA and osmotic stress:H2O2 is the likely intermediary signalling molecule forthe response. The Plant Journal, 22, 87-95

[13]Halliwell B. Reactive species and antioxidants. redoxbiology is a fundamental theme of aerobic life. PlantPhysiology, 141, 312-322

[14]Hare P D, Cress V A, Staden J V. 1999. Proline synthesisand degredation: a model system for elucidating stressrelated signal transduction. Journal of ExperimentalBotany, 50, 413-434

[15]Hojati M, Modarres-sanavy S A M, Karimi M, Ghanati F.2010. Responses of growth and antioxidant systems inCarthamus tinctorius L. under water deficit stress. ActaPhysiologia Plantarum, 33, 105-112

[16]Jiang M Y, Zhang J H. 2002. Water stress induced abscisicacid accumulation triggers the increased generation ofreactive oxygen species and up-regulates the activitiesof antioxidant enzymes in maize leaves. Journal ofExperimental Botany, 53, 2401-2410

[17]Jeffries P, Gianinazzi S, Perotto S, Turnau K, Barea J M.2003. The contribution of arbuscular mycorrhizal fungiin sustainable maintenance of plant health and soilfertility. Biology and Fertility of Soils, 37, 1-16

[18]Kathju S, Vyas S P, Garg B K, Lahiri A N. 1988. Fertilityinduced improvements in performance and metabolismof wheat under different intensities of water stress. In:Proceedings of the International Congress of PlantPhysiology. Society for Plant Physiology andBiochemistry, Watertechnorny Cern, Indian AgriculturalResearch Insltvl’e, New Delhi, India. pp. 854-858

[19]Kennedy A C. 1998. The rhizosphere and spermosphere.In: Sylvia D M, Fuhrmann J J, Hartel P G, Zuberer D A,eds . , Pr inc ipl e s and Appl icat ions of Soi lMicrobiology. Prentice-Hall, Englewood Cliff, NJ. pp.389-407

[20]Kohl D H, Kennelly E J, Zhu Y, Schubert K R, Shearer G.1991. Proline accumulation, nitrogenase (C2H2 reducing)activity and activities of enzymes related to proline metabolism in drought-stressed soybean nodules.Journal of Experimental Botany, 42, 831-837

[21]Leung J, Giraudat J. 1998. Abscisic acid signal transduction.Annual Review of Plant Physiology and PlantMolecular Biology, 49, 199-222

[22]Masoumi H, Darvish F, Daneshian J, Normohammadi G,Habibi D. 2011. Effects of water deficit stress on seedyield and antioxidants content in soybean (Glycine maxL.) cultivars. African Journal of Agricultural Research,6, 1209-1218

[23]Matysik J, Alia A, Bhalu B, Mohanty P. 2002. Molecularmechanism of quenching of reactive oxygen speciesby proline under water stress in plants. CurrentScience, 82, 525-532

[24]Miao Y, Lv D, Wang P, Wang X C, Chen J, Miao C. 2006.An Arabidopsis glutathione peroxidase functions asboth a redox transducer and a scavenger in abscisicacid and drought stress responses. The Plant Cell, 18,2749-2766

[25]Murata Y, Pei Z M, Mori I C, Schroeder J I. 2001. Abscisicacid activation of plasma membrane Ca2+ channels inguard cells requires cytosolic NAD(P)H and isdifferentially disrupted upstream and downstream ofreactive oxygen species production in abi1-1 and abi2-1 protein phosphatase 2C mutants

[26]The Plant Cell, 13,2513-2523

[27]Okon Y, Itzigsohn R. 1995. The development of Azospirillumas a commercial inoculant for improving crop yields.Biotechnology Advances, 13, 415-424

[28]Okon Y, Labandera-Gonzalez C A. 1994. Agronomicapplications of Azospirillum: an evaluation of 20 yearsworldwide field inoculation. Soil Biology andBiochemistry, 26, 1591-1601

[29]Pedersen A L, Felder H C, Rosendahl L. 1996. Effect ofproline on nitrogenase activity in symbiosomes fromroot nodules of soybean (Glycine max L.) subjected todrought stress. Journal of Experimental Botany, 47,1533-1539

[30]Peleg-Grossman S, Volpin H, Levine A. 2007. Root haircurling and rhizobium infection in Medicago trancatulaare mediated by phosphatidylinostide-regulatedendocytosis and reactive oxygen species. Journal ofExperimental Botany, 58, 1637-1649

[31]Perdomo P, Murphy J A, Berkowitz GA. 1996. Physiologicalchanges associated with performance of Kentuckybluegrass cultivars during summer stress. HorticultureScience, 31, 1182-1186

[32]Prasad T K, Anderson M D, Martin B A, Stewart C R. 1994.Evidence for chilling induced oxidative stress in maizeseedliongs and a regulatory role for hydrogen peroxide.The Plant Cell, 6, 65-74

[33]Puckette M C, Weng H, Mahalingam R. 2007. Physiologicaland biochemical responses to acute ozone-inducedoxidative stress in Medicago truncatula. PlantPhysiology and Biochemistry, 45, 70-79

[34]Saharan B S, Nehra V. 2011. Plant Growth PromotingRhizobacteria: A Critical Review. Life Sciences andMedicine Research, LSMR-21, JK

[35]pp. 1-30

[36]Sall K, Sinclair T R. 1991. Soybean genotypic differencesin sensitivity of symbiotic nitrogen fixation to soildehydration. Plant Soil, 133, 31-37

[37]Santos R, Herouart D, Siguad S, Touati D, Puppo A. 2001.Oxidative burst in alfalfa-sinorhizobium melilotisymbiotic interaction. Molecular and Plant MicrobeInteraction, 14, 86-89

[38]Shao H B, Liang Z S, Shao M A, Sun A. 2005. Dynamicchanges of antioxidative enzymes of ten wheatgenotypes at soil water deficits. Colloids and Surfaces(B), 42, 187-195

[39]Somasegaran P, Hoben H J. 1994. Handbook for Rhizobia:Methods in Legumerhizobiu Technology. Springer-Verlag, New York, USA. p. 450.Stajner D, Kevreaan S, G saíc O, Mimica-Dudíc N, ZongliH. 1997. Nitrogen and Azotobacter chroococcumenhance oxidative stress tolerance in sugar beet.Biologia Plantarum, 39, 441-445

[40]Stewart C R, Voetberg G. 1985. Relationship between stressinducedABA and proline accumulations and ABAinducedproline accumulation in excised barley leaves.Plant Physiology, 79, 24-27

[41]Suprunova T, Krugman T, Fahima T, Cheng G, Shams I,Korol A, Nevo E. 2004. Differential expression ofdehydrin genes in wild barley, Hordeum spontaneum,associated with resistance water deficit. Plant CellEnvironment, 27, 1297-1308

[42]Upadhyaya H, Panda S K, Dutta B K. 2008. Variation ofphysiological and oxidative responses in tea cultivarssubjected to elevated water stress followed byrehydration recovery. Acta Physiologia Plantarum, 30,457-468

[43]Urbanek H, Kuzniak-Gebarowska E, Herka K. 1991.Elicitation of defense responses in bean leaves byBotrytis cinerea polygalacturonase. Acta PhysiologiaePlantarum, 13, 43-50

[44]Williamson J D, Scandalios J G. 1992. Differential responseof maize catalase to abscisic acid: Vpl transcriptionalactivator is not required for abscisic acid-regulated Cat1expression. Proceedings of the National Academy ofSciences of the United States of America, 89, 8842-8846

[45]Xing Y, Jia W, Zhang J. 2008. AtMKK1 mediates ABAinducedCAT1 expression and H2O2 production viaAtMPK6-coupled signaling in Arabidopsis. The PlantJournal, 54, 440-451

[46]Yu Q, Rengel Z. 1999. Drought and salinity differentiallyinfluence activities of superoxide dismutases in narrowleafedlupins. Plant Science, 144, 1-11

[47]Zhang J, Cui S, Li J, KirkhamMB. 1995. Protoplasmic factors,antioxidant responses, and chilling resistance in maize.Plant Physiology and Biochemistry, 33, 567-575
[1] YANG Hong-jun, YE Wen-wu, YU Ze, SHEN Wei-liang, LI Su-zhen, WANG Xing, CHEN Jia-jia, WANG Yuan-chao, ZHENG Xiao-bo. Host niche, genotype, and field location shape the diversity and composition of the soybean microbiome[J]. >Journal of Integrative Agriculture, 2023, 22(8): 2412-2425.
[2] XU Lei, ZHAO Tong-hua, Xing Xing, XU Guo-qing, XU Biao, ZHAO Ji-qiu.

Model fitting of the seasonal population dynamics of the soybean aphid, Aphis glycines Matsumura, in the field [J]. >Journal of Integrative Agriculture, 2023, 22(6): 1797-1808.

[3] Mariama KEBBEH, DONG Jing-xian, HUAN Chen, SHEN Shu-ling, LIU Yan, ZHENG Xiao-lin. Melatonin treatment alleviates chilling injury in mango fruit 'Keitt' by modulating proline metabolism under chilling stress[J]. >Journal of Integrative Agriculture, 2023, 22(3): 935-944.
[4] GAO Hua-wei, YANG Meng-yuan, YAN Long, HU Xian-zhong, HONG Hui-long, ZHANG Xiang, SUN Ru-jian, WANG Hao-rang, WANG Xiao-bo, LIU Li-ke, ZHANG Shu-zhen, QIU Li-juan. Identification of tolerance to high density and lodging in short petiolate germplasm M657 and the effect of density on yield-related phenotypes of soybean[J]. >Journal of Integrative Agriculture, 2023, 22(2): 434-446.
[5] QU Zheng, LI Yue-han, XU Wei-hui, CHEN Wen-jing, HU Yun-long, WANG Zhi-gang. Different genotypes regulate the microbial community structure in the soybean rhizosphere[J]. >Journal of Integrative Agriculture, 2023, 22(2): 585-597.
[6] GAO Hua-wei, SUN Ru-jian, YANG Meng-yuan, YAN Long, HU Xian-zhong, FU Guang-hui, HONG Hui-long, GUO Bing-fu, ZHANG Xiang, LIU Li-ke, ZHANG Shu-zhen, QIU Li-juan. Characterization of the petiole length in soybean compact architecture mutant M657 and the breeding of new lines[J]. >Journal of Integrative Agriculture, 2022, 21(9): 2508-2520.
[7] ZHANG Hua, WU Hai-yan, TIAN Rui, KONG You-bin, CHU Jia-hao, XING Xin-zhu, DU Hui, JIN Yuan, LI Xi-huan, ZHANG Cai-ying. Genome-wide association and linkage mapping strategies reveal genetic loci and candidate genes of phosphorus utilization in soybean[J]. >Journal of Integrative Agriculture, 2022, 21(9): 2521-2537.
[8] ZOU Jia-nan, ZHANG Zhan-guo, KANG Qing-lin, YU Si-yang, WANG Jie-qi, CHEN Lin, LIU Yan-ru, MA Chao, ZHU Rong-sheng, ZHU Yong-xu, DONG Xiao-hui, JIANG Hong-wei, WU Xiao-xia, WANG Nan-nan, HU Zhen-bang, QI Zhao-ming, LIU Chun-yan, CHEN Qing-shan, XIN Da-wei, WANG Jin-hui. Characterization of chromosome segment substitution lines reveals candidate genes associated with the nodule number in soybean[J]. >Journal of Integrative Agriculture, 2022, 21(8): 2197-2210.
[9] PAN Wen-jing, HAN Xue, HUANG Shi-yu, YU Jing-yao, ZHAO Ying, QU Ke-xin, ZHANG Ze-xin, YIN Zhen-gong, QI Hui-dong, YU Guo-long, ZHANG Yong, XIN Da-wei, ZHU Rong-sheng, LIU Chun-yan, WU Xiao-xia, JIANG Hong-wei, HU Zhen-bang, ZUO Yu-hu, CHEN Qing-shan, QI Zhao-ming. Identification of candidate genes related to soluble sugar contents in soybean seeds using multiple genetic analyses[J]. >Journal of Integrative Agriculture, 2022, 21(7): 1886-1902.
[10] LIU Chen, TIAN Yu, LIU Zhang-xiong, GU Yong-zhe, ZHANG Bo, LI Ying-hui, NA Jie, QIU Li-juan. Identification and characterization of long-InDels through whole genome resequencing to facilitate fine-mapping of a QTL for plant height in soybean (Glycine max L. Merr.)[J]. >Journal of Integrative Agriculture, 2022, 21(7): 1903-1912.
[11] HUI Fang, XIE Zi-wen, LI Hai-gang, GUO Yan, LI Bao-guo, LIU Yun-ling, MA Yun-tao. Image-based root phenotyping for field-grown crops: An example under maize/soybean intercropping[J]. >Journal of Integrative Agriculture, 2022, 21(6): 1606-1619.
[12] Yeison M QUEVEDO, Liz P MORENO, Eduardo BARRAGÁN. Predictive models of drought tolerance indices based on physiological, morphological and biochemical markers for the selection of cotton (Gossypium hirsutum L.) varieties[J]. >Journal of Integrative Agriculture, 2022, 21(5): 1310-1320.
[13] LIU Jian-long, ZHANG Chen-xiao, LI Tong-tong, LIANG Cheng-lin, YANG Ying-jie, LI Ding-Li, CUI Zhen-hua, WANG Ran, SONG Jian-kun. Phenotype and mechanism analysis of plant dwarfing in pear regulated by abscisic acid[J]. >Journal of Integrative Agriculture, 2022, 21(5): 1346-1356.
[14] TIAN Yu, YANG Lei, LU Hong-feng, ZHANG Bo, LI Yan-fei, LIU Chen, GE Tian-li, LIU Yu-lin, HAN Jia-nan, LI Ying-hui, QIU Li-juan. QTL analysis for plant height and fine mapping of two environmentally stable QTLs with major effects in soybean[J]. >Journal of Integrative Agriculture, 2022, 21(4): 933-946.
[15] LIU Sang-lin, CHENG Yan-bo, MA Qi-bin, LI Mu JIANG Ze, XIA Qiu-ju, NIAN Hai. Fine mapping and genetic analysis of resistance genes, Rsc18, against soybean mosaic virus[J]. >Journal of Integrative Agriculture, 2022, 21(3): 644-653.
No Suggested Reading articles found!