|
|
|
UsingPhospholipid FattyAcid Technique toAnalysis theRhizosphere Specific Microbial Community of Seven Hybrid Rice Cultivars |
ZHU Yu-jing, HU Gui-ping, LIU Bo, XIE Hua-an, ZHENG Xue-fang , ZHANG Jian-fu |
1.Fujian Academy of Agricultural Sciences, Fuzhou 350003, P.R.China
2.Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China |
|
|
摘要 To analyze the intrinsic relationship between rhizosphere microbial community structure and variety of rice, the microbial community structures in rhizosphere of different hybrid rice cultivars were determined with phospholipid fatty acids (PLFA) analysis. Three series of new-breeding hybrid rice cultivars in China were tested in the experiment, IIyouming 86 (II-32A/Minghui 86), IIyouhang 1 (II-32A/Hang 1), and IIyouhang 2 (II-32A/Hang 2) with II-32A as female parent, XinyouHK02 (XinA/HK02) and YiyouHK02 (YXA/HK02) with HK02 as male parent, Chuanyou 167 (ChuanxiangA/MR167) and 44you167 (Hunan44A/MR167) with MR167 as male parent. The results showed that the microbial community in rhizosphere of the hybrid rice comprised bacteria, fungi, actinomycetes, and protozoa, according to the 40 PLFA biomarkers detected. Bacteria were more abundant than fungi and actinomycetes in rhizosphere of the hybrid rice tested. Both sulfate-reducing and methane-oxidizing bacteria were found to exist in the hybrid rice rhizosphere. It was also found that the characteristics of PLFA biomarkers had correlation with the biological traits of rice. The cluster analysis suggested that microbial community structure and activity in rhizosphere were associated with genetic background of the rice cultivar.
Abstract To analyze the intrinsic relationship between rhizosphere microbial community structure and variety of rice, the microbial community structures in rhizosphere of different hybrid rice cultivars were determined with phospholipid fatty acids (PLFA) analysis. Three series of new-breeding hybrid rice cultivars in China were tested in the experiment, IIyouming 86 (II-32A/Minghui 86), IIyouhang 1 (II-32A/Hang 1), and IIyouhang 2 (II-32A/Hang 2) with II-32A as female parent, XinyouHK02 (XinA/HK02) and YiyouHK02 (YXA/HK02) with HK02 as male parent, Chuanyou 167 (ChuanxiangA/MR167) and 44you167 (Hunan44A/MR167) with MR167 as male parent. The results showed that the microbial community in rhizosphere of the hybrid rice comprised bacteria, fungi, actinomycetes, and protozoa, according to the 40 PLFA biomarkers detected. Bacteria were more abundant than fungi and actinomycetes in rhizosphere of the hybrid rice tested. Both sulfate-reducing and methane-oxidizing bacteria were found to exist in the hybrid rice rhizosphere. It was also found that the characteristics of PLFA biomarkers had correlation with the biological traits of rice. The cluster analysis suggested that microbial community structure and activity in rhizosphere were associated with genetic background of the rice cultivar.
|
Received: 14 November 2011
Accepted:
|
Fund: This work was supported by the National Basic Research Program of China (2011CB111607) and the Fujian Funds for Distinguished Young Scientists, China (2009J06010). |
Corresponding Authors:
Correspondence LIU Bo, Tel/Fax: +86-591-87864601, E-mail: liubofaas@163.com
E-mail: liubofaas@163.com
|
About author: ZHU Yu-jing, Mobile: 13960770801, Tel: +86-591-87882571, E-mail: zyjingfz@163.com; HU Gui-ping, E-mail: hugp_2007@163.com; |
Cite this article:
ZHUYu-jing , HU Gui-ping, LIU Bo, XIE Hua-an, ZHENGXue-fang , ZHANG Jian-fu.
2012.
UsingPhospholipid FattyAcid Technique toAnalysis theRhizosphere Specific Microbial Community of Seven Hybrid Rice Cultivars. Journal of Integrative Agriculture, 12(11): 1817-1827.
|
[1]Arab H G D E, Vlich V, Sikora R A. 2001. The use ofphospholipids fatty acids (PLFA) in the determinationof rhizosphere specific microbial communities of twowheat cultivars. Plant and Soil, 228, 291-297[2]Balser T C, Firestone M K. 2005. Linking microbialcommunity composition and soil processes in aCalifornia annual grassland and mixed-conifer forest.Biogeochemistry, 73, 395-415[3]Brookes P C, Cayuela M L, Contin M, Nobili D M, KemmittS J, Mondini C. 2007. The mineralisation of fresh andhumified soil organic matter by the soil microbialbiomass. Waste Management, 28, 716-722[4]Briones AM, Okabe S, Umemiya Y, Ramsing N B, ReichardtW, Okuyama H. 2002. Influence of different cultivarson populations of ammonia-oxidizing bacteria in theroot environment of rice. Applied EnvironmentalMicrobiology, 68, 3067-3075[5]Chen LY, Xiao Y H, TangWB, Lei D Y. 2007. Practices andprospects of super hybrid rice breeding. Rice Science,14, 71-77[6](in Chinese)Cocking E C. 2003. Endophytic colonisation of plant rootsby nitrogen-fixing bacteria. Plant Soil Environment,252, 169-175[7]Frostegfird A, Baath E. 1996. The use of phospholipid fattyacid analysis to estimate bacterial and fungal biomassin soil. Biology and Fertility of Soils, 22, 59-65[8]Garbeva P, van Veen J A, van Elsas J D. 2004. Microbialdiversity in soil: selection microbial populations byplant and soil type and implications for diseasesuppressiveness. Annual Review of Phytopathology,42, 243-270[9]Haubert D, GgblomM M H, Scheu S, Ruess L. 2008. Effectsof temperature and life stage on the fatty acidcomposition of Collembola. European Journal of SoilBiology, 44, 213-219[10]Hedrick D B, Peacock A, Stephen J R, Macnaughton S J,Bruggemann J, White D C. 2000. Measuring soilmicrobial community diversity using polar lipid fattyacid and denaturing gradient gel electrophoresis data.Journal of Microbiological Methods, 41, 235-248[11]Hill G T, Mitkowski N A, Aldrich-Wolfe L, Emele L R,Jurkonie D D, Ficke A, Maldonado-Ramirez S, Lynch ST, Nelson E B. 2000. Methods for assessing thecomposi t ion and diversity of soi l microbialcommunities. Applied Soil Ecology, 15, 25-36[12]Hinojosa M B, Carreira, J A, Garcia-Ruiz R, Dick R P. 2005.Microbial response to heavy metal-polluted soils:community analysis from phospholipid-linked fattyacids and ester-linked fatty acids extracts. Joural ofEnvironment Quality, 34, 1789-1800[13]Ikeda S, Ytow N, Ezura H,Minamisawa K, Fujimura T. 2006.Soil microbial community analysis in the environmentalrisk asses sment of t ransgenic plants. PlantBiotechnology, 23, 131-151[14]Kimura M, Asakawa S. 2006. Comparison of communitystructures of microbiota at main habitats in rice fieldecosystems based on phospholipid fatty acid analysis.Biology and Fertility of Soils, 43, 20-29[15]Kong C H, Wang P, Gu Y, Xu X H, Wang M L. 2008. Fateand impact on microorganisms of rice allelochemicalsin paddy soil. Journal Agricultural and FoodChemistry, 56, 5043-5049[16](in Chinese)Kourtev P S, Ehrenfeld J G, GgblomM H. 2002. Exotic plantspecies alter the microbial community structure andfunction in the soil. Ecology, 83, 3152-3166[17]Kourtev P S, Ehrenfeld J G, HaggblomM. 2003. Experimentanalysis of the effect of exotic and native plant specieson st ructure and func t ion of soi l microbi a lcommunities. Soil Biology and Biochemistrym, 35,895-905[18]Liu B, Hu G P, Zheng X F, Zhang J F, Xie HA. 2010.Analysison microbial diversity in the rhizosphere of rice byphospholipid fatty acids biomarkers. Chinese Journalof Rice Science, 24, 278-288[19](in Chinese)Lu Y, Rosencrantz D, LiesackW, Conrad R. 2006. Structureand activity of bacterial community inhabiting rice rootsand the rhizosphere. Environmental Microbiology, l8,1351-1360. (in Chinese)[20]Mubyana-John T, Wutor V C, Yeboah S O, Ringrose S.2007. Fire and its influence on microbial communitystructure and soil biochemical properties in theOkavango Delta, Botswana. Scientific Research andEssay, 2, 47-54[21]Myers R T, Zak D R, White D C, Peacock A. 2001.Landscape-level patterns of microbial communitycomposition and substrate use in upland forestecosystems. Soil Science Society of America Journal,65, 359-367[22]Nancy J R, Mary E S, Richard P D, David D M. 2000. Use oflength heterogeneity PCR and fatty acid methyl esterprofiles to characterize microbial communities in soil.Applied and Environmental Microbiology, 66, 1668-1675[23]Olsson P A, Thingstrup I, Jakobsen I, Baath E. 1999.Estimation of the biomass of arbuscular mycorrhizalfungi in a linseed field. Soil Biology and Biochemistry,31, 1879-1887[24]Peng P Q, Qiu S J, Tong C L, Ren X E. 2007. Effects of longtermapplication of fertilizers on soil microbial biomassnitrogen and organic nitrogen components insubtropical paddy soi ls. Chinese Journal ofEnvionmental Science, 28, 1816-1821 (in Chinese)[25]Salomonovaa S, Lama?ovab J, Rulikb M, Rol?ikc J, ?apa L,Bedna a P, Bartak P. 2003. Determination of phospholipidfat ty acids in sediments. Acta Universi tati sPalackianae Olomucensis Facultas Rerum Chemica,42, 39-49[26]Sarah J M, John R S, Albert D V, Gregory A D, Chang Y J,David C W. 1999. Microbial population changes duringbioremediation of an experimental oil spill. Applied andEnvironmental Microbiology, 65, 3566-3574[27]Sass H, Overmann J, Rutters H, Babenzien H, Cypionka H.2004. Desulfosporomusa polytropa gen. nov., sp. nov.,a novel sulfate-reducing bacterium from sediments ofan oligotrophic lake. Archives of Microbiology, 182,204-211[28]Schutter M E, Dick R P. 2000. Comparison of fatty acidmethyl ester (FAME) methods for characterizingmicrobial communities. Soil Science Society of AmericaJournal, 64, 1659-1668[29]Siciliano S D, Theoret C M, Freitas J R D, Hucl P J, GermidaJ J. 1998. Differences in the microbial communitiesassociated with the roots of different cultivars of canolaand wheat. Canadian Journal of Microbiology, 44,844-851[30]Tetsuya D, Jun A, Fumitaka S, Shigenori M. 2011. Study onrhizosphere bacterial community in lowland rice grownwith organic fertilizers by using PCR-denaturing gradientgel electrophoresis. Plant Root, 5, 5-16[31]Vestal J R, White D C. 1989. Lipid analysis in microbialecology: quantitative approaches to the study ofmicrobial communities. Bioscience, 39, 535-541[32]Waldrop M P, Balser T C, Firestone M K. 2000. Linkingmicrobial community composition to function in atropical soil. Soil Biology and Biochemistry, 32, 1837-1846[33]Wang G H, Jin J, Chen X L, Liu J D, Liu X B, Herbert S J.2007. Biomass and catabolic diversity of microbialcommunities with long-term restoration, bare fallow andcropping history in Chinese Mollisols. Plant Soil andEnvironment, 53, 177-185[34](in Chinese)Wieland G, Neumann R, Backhaus H. 2001. Variation ofmicrobial communities in soil, rhizosphere, andrhizoplane in response to crop species, soil type, andcrop development. Applied and EnvironmentalMicrobiology, 67, 5849-5854[35]Zhang P, Zheng J, Pan G, Zhang X, Li L, Tippkotter R.2007. Changes in microbial community structure andfunction within particle size fractions of a paddy soilunder different long-term fertilization treatment from theTai Lake region, China. Colloids Surf B Biointerfaces,58, 264-270[36]Zhang Q C, Wang G H. 2005. Studies on nutrient uptake ofrice and characteristics of soil microorganisms in a longtermfertilization experiments for irrigated rice. Journalof Zhejiang University Science (B), 6, 147-154 (in Chinese)[37]Zhang Q C, Wang G H, Yao H Y. 2007. Phospholipid fattyacid patterns of microbial communities in paddy soilunder different fertilizer treatments. Chinese Journalof Environmental Science, 19, 55-59 (in Chinese) |
No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|