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Journal of Integrative Agriculture  2012, Vol. 12 Issue (4): 521-·527    DOI: 10.1016/S1671-2927(00)8571
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Characterization and Molecular Mapping of a Stripe Rust Resistance Gene in Synthetic Wheat CI110
 REN Qiang, LIU Hui-juan, ZHANG Zeng-yan, FENG Jing, XU Shi-chang, PU Zong-jun , XIN Zhiyong
1.The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
3 .Crop Research Institute, Sichun Academy of Agricultural Sciences, Chengdu 610066, P.R.China
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摘要  Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.). To diversify stripe rust-resistant resources for wheat breeding programs, a CIMMYT synthetic wheat line CI110 was identified to be resistant to 28 isolates of Pst, including 6 Chinese prevalent races CYR28-CYR33. Genetic analysis indicated that a single dominant gene was responsible for the stripe rust resistance in CI110, temporarily designated YrC110. A molecular map, harboring YrC110 and 9 linked SSR markers, was constructed through simple sequence repeat (SSR), and bulked segregant analysis. These linked markers and YrC110 were assigned on the short arm of chromosome 1B using the Chinese Spring nullisomic-tetrasomic and ditelosomic stocks. Gene postulation based on seedling reaction patterns to 30 Pst isolates suggested that the resistance gene YrC110 seemed different from the other known resistance genes tested, such as Yr9, Yr10, Yr15, Yr24, and Yr26/YrCH42. Four SSR markers Xbarc187150, Xgwm18227, Xgwm11223, and Xbarc240292 distinguished YrC110 from Yr10, Yr15, Yr24, and Yr26/YrCH42, and could be used as diagnostic ones for YrC110 in wheat resistant breeding programs against stripe rust.

Abstract  Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.). To diversify stripe rust-resistant resources for wheat breeding programs, a CIMMYT synthetic wheat line CI110 was identified to be resistant to 28 isolates of Pst, including 6 Chinese prevalent races CYR28-CYR33. Genetic analysis indicated that a single dominant gene was responsible for the stripe rust resistance in CI110, temporarily designated YrC110. A molecular map, harboring YrC110 and 9 linked SSR markers, was constructed through simple sequence repeat (SSR), and bulked segregant analysis. These linked markers and YrC110 were assigned on the short arm of chromosome 1B using the Chinese Spring nullisomic-tetrasomic and ditelosomic stocks. Gene postulation based on seedling reaction patterns to 30 Pst isolates suggested that the resistance gene YrC110 seemed different from the other known resistance genes tested, such as Yr9, Yr10, Yr15, Yr24, and Yr26/YrCH42. Four SSR markers Xbarc187150, Xgwm18227, Xgwm11223, and Xbarc240292 distinguished YrC110 from Yr10, Yr15, Yr24, and Yr26/YrCH42, and could be used as diagnostic ones for YrC110 in wheat resistant breeding programs against stripe rust.
Keywords:  synthetic wheat      Puccinia striiformis f. sp. tritici      resistance gene      simple sequence repeat (SSR) marker      gene postulation  
Received: 17 February 2011   Accepted:
Fund: 

This study was funded by the 863 Program of China (2006AA100120).

Corresponding Authors:  Correspondence ZHANG Zeng-yan, Tel: +86-10-82108781, E-mail: zhangzy@mail.caas.net.cn     E-mail:  zhangzy@mail.caas.net.cn

Cite this article: 

REN Qiang, LIU Hui-juan, ZHANG Zeng-yan, FENG Jing, XU Shi-chang, PU Zong-jun , XIN Zhiyong. 2012. Characterization and Molecular Mapping of a Stripe Rust Resistance Gene in Synthetic Wheat CI110. Journal of Integrative Agriculture, 12(4): 521-·527.

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