Please wait a minute...
Journal of Integrative Agriculture  2012, Vol. 12 Issue (3): 359-367    DOI: 10.1016/S1671-2927(00)8553
GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS Advanced Online Publication | Current Issue | Archive | Adv Search |
Genetic Analysis of Cold Tolerance at Seedling Stage and Heat Tolerance atAnthesis in Rice (Oryza sativa L.)
 CHENG Li-rui, Veronica Uzokwe, WANG Yun, ZHU Linghua
1.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural
Sciences, Beijing 100081, P.R.China
2.Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China
3.International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  A set of 240 introgression lines derived from the advanced backcross population of a cross between a japonica cultivar,Xiushui 09, and an indica breeding line, IR2061, was developed to dissect QTLs affecting cold tolerance (CT) at seedlingstage and heat tolerance (HT) at anthesis. Survival rate of seedlings (SRS) and spikelet fertility (SF), the index traits of CTand HT, showed significant differences between the two parents under stresses. A total of four QTLs (qSRS1, qSRS7,qSRS11a and qSRS11b) for CT were identified on chromosomes 1, 7, 11, and the Xiushui 09 alleles increased SRS at all lociexcept qSRS7. Four QTLs for SF were identified on chromosomes 4, 5, 6, and 11. These QTLs could be classified into twomajor types based on their behaviors under normal and stress conditions. The first was QTL expressed only under normalcondition; and the second QTL was apparently stress induced and only expressed under stress. Among them, two QTLs(qSF4 and qSF6) which reduced the trait difference between heat stress and normal conditions must have contributed toHT because of their obvious contribution to trait stability, and the IR2061 allele at the qSF6 and the Xiushui 09 allele at the qSF4improved HT, respectively. No similar QTL was found between CT at seedling stage and HT at anthesis. Therefore, it ispossible to breed a new variety with CT and HT by pyramiding the favorable CT- and HT-improved alleles at above locifrom Xiushui 09 and IR2061, respectively, through marker-assisted selection (MAS).

Abstract  A set of 240 introgression lines derived from the advanced backcross population of a cross between a japonica cultivar,Xiushui 09, and an indica breeding line, IR2061, was developed to dissect QTLs affecting cold tolerance (CT) at seedlingstage and heat tolerance (HT) at anthesis. Survival rate of seedlings (SRS) and spikelet fertility (SF), the index traits of CTand HT, showed significant differences between the two parents under stresses. A total of four QTLs (qSRS1, qSRS7,qSRS11a and qSRS11b) for CT were identified on chromosomes 1, 7, 11, and the Xiushui 09 alleles increased SRS at all lociexcept qSRS7. Four QTLs for SF were identified on chromosomes 4, 5, 6, and 11. These QTLs could be classified into twomajor types based on their behaviors under normal and stress conditions. The first was QTL expressed only under normalcondition; and the second QTL was apparently stress induced and only expressed under stress. Among them, two QTLs(qSF4 and qSF6) which reduced the trait difference between heat stress and normal conditions must have contributed toHT because of their obvious contribution to trait stability, and the IR2061 allele at the qSF6 and the Xiushui 09 allele at the qSF4improved HT, respectively. No similar QTL was found between CT at seedling stage and HT at anthesis. Therefore, it ispossible to breed a new variety with CT and HT by pyramiding the favorable CT- and HT-improved alleles at above locifrom Xiushui 09 and IR2061, respectively, through marker-assisted selection (MAS).
Keywords:  cold tolerance      heat tolerance      advanced backcross population      QTL mapping      rice  
Received: 10 February 2011   Accepted:
Fund: 

The work was funded by the Projectof the 863 Program (2010AA101803) and the 948 Programof China (2006-G51 and 2010-G2B).

Corresponding Authors:  Correspondence XU Jian-long, Tel: +86-10-82105854, Fax: +86-10-82108559, E-mail: xujlcaas@yahoo.com.cn     E-mail:  xujlcaas@yahoo.com.cn

Cite this article: 

CHENG Li-rui, Veronica Uzokwe, WANG Yun, ZHU Linghua. 2012. Genetic Analysis of Cold Tolerance at Seedling Stage and Heat Tolerance atAnthesis in Rice (Oryza sativa L.). Journal of Integrative Agriculture, 12(3): 359-367.

[1]Andaya V C, Mackill D J. 2003. Mapping of QTLs associatedwith cold tolerance during the vegetative stage in rice.Journal of Experimental Botany, 54, 2579-2585.

[2]Andaya V C, Tai T H. 2006. Fine mapping of the qCTS12locus, a major QTL for seedling CT in rice. Theoreticaland Applied Genetics, 113, 467-475.

[3]Blum A, Klueva N, Nguyen H T. 2001. Wheat cellularthermotolerance is related to yield under heat stress.Euphytica, 117, 117-123.

[4]Cao L Y, Zhu J, Zhao S T, He L B, Yan Q C. 2002. MappingQTLs for heat tolerance in a HD population from indicajaponicacross of rice (Oryza sativa L.). Journal ofAgricultural Biotechnology, 10, 210-214. (in Chinese)

[5]Chen L, Lou Q J, Sun Z X, Xing Y Z, Yu X Q, Luo L J. 2006.QTL mapping of low temperature germinability in rice.Chinese Journal of Rice Science, 20, 159-164. (in Chinese)

[6]Chen Q Q, Yu S B, Li C H, Mou T M. 2008. Identification ofQTLs for heat tolerance at flowering stage in rice.Scientia Agricultura Sinica, 41, 315-321. (in Chinese)

[7]Churchill G A, Doerge R W. 1994. Empirical threshold valuesfor quantitative trait mapping. Genetics, 138, 963-971.

[8]Dawe D C, Pandey S, Nelson A. 2010. Emerging trends andspatial patterns of rice production. In: Pandey S, ByerleeD, Dawe D C, Dobermann A, Mohanty S, Rozelle S,Hardy B, eds., Rice in Global Economy: StrategicRresearch and Policy Issues for Food Security.International Rice Research Institute, Los Banos,Philippines. pp. 15-35.

[9]Dilday R H. 1990. Contribution of ancestral lines in thedevelopment of new cultivars of rice. Crop Science,30, 905-911.

[10]FAO (Food and Agriculture Organization). 2010. WorldCensus of Agriculture. [2011-1-20]. http://www.fao.org/economic/ess/world-census-of-agriculture/en/

[11]Han L Z, Zhang Y Y, Qiao Y L, Cao G L, Zhang S Y, Kim J H,Koh H J. 2006. Genetic and QTL analysis for lowtemperaturevigor of germination in rice. Acta GeneticaSinica, 33, 998-1006. (in Chinese)

[12]Henderson M F, Yeh B P, Exner B. 1958. Further evidenceof structural differentiation in the chromosomes as acause of sterility in inter-varietal hybrids in rice.Cytologia, 24, 415-422.

[13]Ikehashi H, Araki H. 1986. Genetics of F1 sterility in remotecrosses of rice. In: Rice Genetics. International RiceResearch Institute, Los Banos, Philippines. pp. 119-130.

[14]Jacobs B C, Pearson C J. 1999. Growth, development andyield of rice in response to cold temperature. Journalof Agronomy Crop Science, 182, 79-88.

[15]Jagadish S V K, Cairns J, Lafitte R, Wheeler T R, Price A H,Craufurd P Q. 2010. Genetic analysis of heat toleranceat anthesis in rice. Crop Science, 50, 1633-1641.

[16]Jagadish S V K, Craufurd P Q, Wheeler T R. 2007. Hightemperature stress and spikelet fertility in rice (Oryzasativa L.). Journal of Experimental Botany, 58, 1627-1635.

[17]Jeong E G, Yea J D, Baek M K, Moon H P, Choi H C, YoonK M, Ajn S N. 2000. Estimation of critical temperaturefor traits related to cold tolerance in rice. Jorean Journal Breeding, 32, 363-368.

[18]Ji S L, Jiang L, Wang Y H, Liu S J, Liu X, Zhai H Q, YoshimuraA, Wan J M. 2008. QTL and epistasis for low temperaturegerminability in rice. Scientia Agricultura Sinica, 34,551-556. (in Chinese)

[19]Jiang L, Xun M M, Wang J K, Wan J M. 2008. QTL analysisof cold tolerance at seedling stage in rice (Oryza sativaL.) using recombination inbred lines. Journal of CerealScience, 48, 173-179.

[20]Li H B, Wang J, Liu A M, Liu K D, Zhang Q F, Zou J S. 1997.Genetic basis of low-temperature-sensitive sterility inindica-japonica hybrids of rice as determined by RFLPanalysis. Theoretical and Applied Genetics, 95, 1092-1097.

[21]Li H B, Zhang Q F, Liu A M, Zou J S, Chen Z M. 1996. Agenetic analysis of low temperature sensitive sterilityin indica-japanica hybrids. Plant Breeding, 115, 305-309.

[22]Li Z K, Pinson S R M, Paterson A H, Park W D, Stansel J W.1997. Genetics of hybrid sterility and hybrid breakdownin an inter-subspecific rice (Oryza sativa L.) population.Genetics, 145, 1139-1148.

[23]Liu F X, Sun C Q, Tan L, Fu Y C, Li D, Wang X K. 2003.Identification and mapping of quantitative trait locicontrolling cold-tolerance of Chinese common wild rice(O. rufipogon Griff.) at booting to flowering stages.Chinese Science Bulletin, 48, 2068-2071.

[24]Lou Q J, Chen L, Sun Z X, Xing Y Z, Li J, Xu X Y, Mei H W,Luo L J. 2007. A major QTL associated with CT atseedling stage in rice (Oryza sativa L.). Euphytica, 15,87-94.

[25]Lu C G, Wang C L, Zong S Y, Zhao L, Zou J S. 2002. Effectsof temperature on fertility and seed set inintersubspecific hybrid rice (Oryza sativa L.). ScientiaAgricultura Sinica, 28, 499-504. (in Chinese)

[26]Mackil D J, Lei X. 1997. Genetic variation for traits relatedto temperate adaptation of rice cultivars. Crop Science,37, 1340-1346.

[27]Manly K F, Olson J M. 1999. Overview of QTL mappingsoftware and introduction to map manager QTL.Mammalian Genome, 10, 327-334.

[28]Matsui T, Omasa K. 2002. Rice (Oryza sativa L.) cultivarstolerant to high temperature at flowering: Anthercharacteristics. Annals of Botany, 8, 683-687.

[29]Nakagahra M, Okuno K, Vaughan D. 1997. Rice geneticresources: history, conservation, investigativecharacterization and use in Japan. Plant MoleuclarBiology, 35, 69-77.

[30]Nakagawa H, Horie T, Matsui T. 2003. Effects of ClimateChange on Rice Production and AdaptiveTechnologies. International Rice Research Institute,Manila, Philippines.Nishida I, Murata N. 1996. Chilling sensitivity in plantsand cyanobacteria: The crucial contribution of membranelipids. Annual Review of Plant Physiology and PlantMolecular Biology, 47, 541-568.

[31]Oh C S, Choi Y H, Lee S J, Yoon D B, Moon H P, Ahn S N.2004. Mapping of quantitative trait loci for coldtolerance in weedy rice. Breeding Science, 54, 373-380.

[32]Oka H I. 1988. Origin of Cultivated Rice. Japan ScientificSociety Press, Tokyo.Rang Z W, Jagadish S V K, Zhou Q M, Craufurd P Q, HeuerS. 2011. Effect of high temperature and water stress onpollen germination and spikelet fertility in rice.Environmental and Experimental Botany, 70, 58-65.

[33]Satake T, Yoshida S. 1978. High temperature inducedsterility in indica rices at flowering. Japanese Journalof Crop Science, 47, 6-17.

[34]Solomon S, Qin D, Manning M, Averyt K, Tignor M M B,Miller H L, Cheng Z. 2007. Climate Change 2007: thePhysical Science Basis. Contribution of WorkingGroup 1 to the Fourth Change Assignment Report ofthe Intergovernmental Panel on Climate Change.Cambridge University Press, New York, USA.

[35]Suh J P, Jeung J U, Lee J I, Choi Y H, Yea J D, Virk P S,Mackill D J, Jena K K. 2010. Identification and analysisof QTLs controlling CT at the reproductive stage andvalidation of effective QTLs in cold-tolerant genotypesof rice (Oryza sativa L.). Theoretical and AppliedGenetics, 120, 985-995.

[36]Tai T H, Tanksley S D. 1990. A rapid and inexpensive methodfor isolation of total DNA from dehydrated plant tissue.Plant Molecular Biology Reporter, 8, 297-303.

[37]Temnykh S, DeClerck G, Lukashova A, Lipovich L,Cartinhour S, McCouch S. 2001. Computational andexperimental analysis of microsatellites in rice (Oryzasativa L.): Frequency, length variation, transposonassociations, and genetic marker potential. GenomeResearch, 11, 1441-1452.

[38]Vergara B S. 1976. Physiological and morphologicaladaptability of rice varieties to climate. In: Climate andRice. International Rice Research Institute, Los Banos,Philippines, pp. 67-83.

[39]Wang D L, Zhu J, Li Z K, Paterson A H. 1999. MappingQTLs with epistatic effects and QTL×environmentinteractions by mixed linear model approaches.Theoretical and Applied Genetics, 99, 1255-1264.

[40]Xu L M, Zhou L, Zeng Y W, Wang F M, Zhang H L, ShenS Q, Li Z C. 2008. Identification and mapping ofquantitative trait loci for CT at the booting stage in ajaponica rice near-isogenic line. Plant Science, 174,340-347.

[41]Yang T F, Liu B. 2009. Progress on the identification ofQTL for heat tolerance in rice (Oryza sativa L.).Guangdong Journal of Rice Science, 6, 16-20. (in Chinese)

[42]Zeng Y W, Yang S M, Cui H, Yang X J, Xu L M, Du J, Pu XY, Li Z C, Cheng Z Q, Huang X Q. 2009. QTLs of coldtolerance-related traits at the booting stage for NILRILsin rice revealed by SSR. Genes and Genomics, 31,143-154.

[43]Zhang G L, Chen L Y, Xiao G Y, Xiao Y H, Chen X B, Zhang S T. 2009. Bulked segregant analysis to detect QTLrelated to heat tolerance in rice (Oryza sativa L.) usingSSR markers. Agricultural Sciences in China, 8, 482-487.

[44]Zhang Z H, Su L, Li W, Chen W, Zhu Y G. 2005. A majorQTL conferring CT at the early seedling stage usingrecombinant inbred lines of rice (Oryza sativa L.). PlantScience, 168, 527-534.

[45]Zhao Z G, Jing L, Xiao Y H, Zhang W W, Zhai H Q, Wan JM. 2006. Identification of QTLs for heat tolerance atthe booting stage in rice (Oryza sativa L.). ActaAgronomica Sinica, 32, 640-644. (in Chinese)

[46]Zhou L, Zeng Y W, Zheng W W, Tang B, Yang S M, ZhangH L, Li J J, Li Z C. 2010. Fine mapping a QTL, qCTB7,for cold tolerance at the booting stage on ricechromosome 7 using a near-isogenic line. Theoreticaland Applied Genetics, 121, 893-905.

[47]Zhu C L, Xiao Y H, Wang C M, Jiang L, Zhai H Q, Wan J M.2005. Mapping QTL for heat-tolerance at grain fillingstage in rice. Rice Science, 12, 33-38.
[1] Gaozhao Wu, Xingyu Chen, Yuguang Zang, Ying Ye, Xiaoqing Qian, Weiyang Zhang, Hao Zhang, Lijun Liu, Zujian Zhang, Zhiqin Wang, Junfei Gu, Jianchang Yang. An optimized strategy of nitrogen-split application based on the leaf positional differences in chlorophyll meter readings[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2605-2617.
[2] Xiaogang He, Zirong Li, Sicheng Guo, Xingfei Zheng, Chunhai Liu, Zijie Liu, Yongxin Li, Zheming Yuan, Lanzhi Li. Epistasis-aware genome-wide association studies provide insights into the efficient breeding of high-yield and high-quality rice[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2541-2556.
[3] Myeong-Hyeon Min, Aye Aye Khaing, Sang-Ho Chu, Bhagwat Nawade, Yong-Jin Park. Exploring the genetic basis of pre-harvest sprouting in rice through a genome-wide association study-based haplotype analysis[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2525-2540.
[4] Peng Xu, Mengdie Jiang, Imran Khan, Muhammad Shaaban, Hongtao Wu, Barthelemy Harerimana, Ronggui Hu. Regulatory potential of soil available carbon, nitrogen, and functional genes on N2O emissions in two upland plantation systems[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2792-2806.
[5] Hui Fang, Xiuyi Fu, Hanqiu Ge, Mengxue Jia, Jie Ji, Yizhou Zhao, Zijian Qu, Ziqian Cui, Aixia Zhang, Yuandong Wang, Ping Li, Baohua Wang. Genetic analysis and candidate gene identification of salt tolerancerelated traits in maize[J]. >Journal of Integrative Agriculture, 2024, 23(7): 2196-2210.
[6] Bin Lei, Jiale Shao, Feng Zhang, Jian Wang, Yunhua Xiao, Zhijun Cheng, Wenbang Tang, Jianmin Wan. Genetic analysis and fine mapping of a grain size QTL in the small-grain sterile rice line Zhuo201S[J]. >Journal of Integrative Agriculture, 2024, 23(7): 2155-2163.
[7] Hanzhu Gu, Xian Wang, Minhao Zhang, Wenjiang Jing, Hao Wu, Zhilin Xiao, Weiyang Zhang, Junfei Gu, Lijun Liu, Zhiqin Wang, Jianhua Zhang, Jianchang Yang, Hao Zhang.

The response of roots and the rhizosphere environment to integrative cultivation practices in paddy rice [J]. >Journal of Integrative Agriculture, 2024, 23(6): 1879-1896.

[8] Jianjun Wang, Yanan Shao, Xin Yang, Chi Zhang, Yuan Guo, Zijin Liu, Mingxun Chen.

Heterogeneous expression of stearoyl-acyl carrier protein desaturase genes SAD1 and SAD2 from Linum usitatissimum enhances seed oleic acid accumulation and seedling cold and drought tolerance in Brassica napus [J]. >Journal of Integrative Agriculture, 2024, 23(6): 1864-1878.

[9] Yuguang Zang, Gaozhao Wu, Qiangqiang Li, Yiwen Xu, Mingming Xue, Xingyu Chen, Haiyan Wei, Weiyang Zhang, Hao Zhang, Lijun Liu, Zhiqin Wang, Junfei Gu, Jianchang Yang.

Irrigation regimes modulate non-structural carbohydrate remobilization and improve grain filling in rice (Oryza sativa L.) by regulating starch metabolism [J]. >Journal of Integrative Agriculture, 2024, 23(5): 1507-1522.

[10] Luqi Jia, Yongdong Dai, Ziwei Peng, Zhibo Cui, Xuefei Zhang, Yangyang Li, Weijiang Tian, Guanghua He, Yun Li, Xianchun Sang.

The auxin transporter OsAUX1 regulates tillering in rice (Oryza sativa) [J]. >Journal of Integrative Agriculture, 2024, 23(5): 1454-1467.

[11] Chaoyue Pang, Ling Jin, Haoyu Zang, Damalk Saint-Claire S. Koklannou, Jiazhi Sun, Jiawei Yang, Yongxing Wang, Liang Xu, Chunyan Gu, Yang Sun, Xing Chen, Yu Chen. Establishment of a system for screening and identification of novel bactericide targets in the plant pathogenic bacterium Xanthomonas oryzae pv. oryzae using Tn-seq and SPR[J]. >Journal of Integrative Agriculture, 2024, 23(5): 1580-1592.
[12] Keanning Li, Bingxing An, Mang Liang, Tianpeng Chang, Tianyu Deng, Lili Du, Sheng Cao, Yueying Du, Hongyan Li, Lingyang Xu, Lupei Zhang, Xue Gao, Junya LI, Huijiang Gao.

Prescreening of large-effect markers with multiple strategies improves the accuracy of genomic prediction [J]. >Journal of Integrative Agriculture, 2024, 23(5): 1634-1643.

[13] Minghao Cai, Xuhui Li, Zhi Liang, Jie Wang, Delin Li, Zhipeng Yuan, Riliang Gu, Jianhua Wang, Li Li.

qSTA2-2, a novel QTL that contributes to seed starch synthesis in Zea mays L. [J]. >Journal of Integrative Agriculture, 2024, 23(4): 1118-1133.

[14] Junnan Hang, Bowen Wu, Diyang Qiu, Guo Yang, Zhongming Fang, Mingyong Zhang.

OsNPF3.1, a nitrate, abscisic acid and gibberellin transporter gene, is essential for rice tillering and nitrogen utilization efficiency [J]. >Journal of Integrative Agriculture, 2024, 23(4): 1087-1104.

[15] Shuang Cheng, Zhipeng Xing, Chao Tian, Mengzhu Liu, Yuan Feng, Hongcheng Zhang.

Optimized tillage methods increase mechanically transplanted rice yield and reduce the greenhouse gas emissions [J]. >Journal of Integrative Agriculture, 2024, 23(4): 1150-1163.

No Suggested Reading articles found!