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Table of Content

    10 May 2005, Volume 38 Issue 05
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES
    Isolation, Identification and Earliness Effect Analysis of Rice Dominant Earliness Gene Ef-cd
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    Scientia Agricultura Sinica. 2005, 38(05):  859-864 . 
    Abstract ( 1100 )   PDF (590KB) ( 838 )   Save
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    Ef-cd gene is a dominant earliness gene located on the short arm of rice chromosome 3. In this paper, through continuous backcross, self-pollination and molecular marker assisted selection, individual Ef-cd gene was isolated and nearly isogenic early-maturing lines were constructed by using a genic male-sterile and early-maturing indica line 6442S-7 as donor, and late-maturing indica lines Minghui 63 (MH 63), Shuhui 881 (SH 881) and Shuhui 527 (SH 527) as recipients (genetic background), respectively. Further, it was found out that Ef-cd gene could generally advance rice to head 11-14 days earlier. So, it is considered that Ef-cd gene has an important role in rapid developing early-maturing and super high-yielding rice varieties.
    Effect of Wx-A1, Wx-B1 and Wx-D1 Protein on Starch Properties and Chinese Fresh Noodle Quality
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    Scientia Agricultura Sinica. 2005, 38(05):  865-873 . 
    Abstract ( 1124 )   PDF (317KB) ( 1357 )   Save
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    Starch properties play an important role in Chinese noodle quality. Eight genotypes from Japanese cultivar 'Kanto 107'/Chinese cultivar 'Baihuo' differing in the presence and absence of three Waxy proteins, Wx-A1, Wx-B1, and Wx-D1, were used to determine the effect of Waxy protein deficiencies on amylose content, starch pasting properties and Chinese fresh noodle quality by using the fractionation and reconstitution procedures. The results showed that amylose content and starch pasting properties were influenced significantly by different Waxy protein. For amylose content, it can be ranked as type 8 (waxy wheat, three proteins null)>5 (Wx-B1 and Wx-D1 null)>7 (Wx-A1 and Wx-B null)>6 (Wx-A and Wx-D null) >3 (Wx-B1)≌4 (Wx-D1)>2 (Wx-A1). The rank for peak viscosity is 8, 3, 5, 4, 6, 7, 2. Types with Wx-B1 null or -D1 null performed the best noodle-making quality. Waxy type has higher peak viscosity and breakdown than other types, while it has lower breakdown, pasting temperature and peak time. Amylose content was significantly and positively correlated with TPA parameters hardness, gumminess, and chewiness of cooked noodle (r = 0.83 - 0.87, P<0.01). It also had a significant and negative influence on noodle springiness, adhesiveness, cohesiveness, and resilience (r = -0.53 - -0.83). It was found that a positive and significant correlation among starch pasting property parameters such as hold though, final viscosity, setback, peak time, and pasting temperature and color, appearance, firmness, elasticity, stickiness, flavor and total score of cooked noodle (r = 0.53 - 0.91). Breakdown had a significant and negative influence on those noodle quality traits (r = -0.66 - -0.74).
    Transformation and Expression of High Molecular Weight Glutenin Subunit Genes in Common Wheat (Triticum aestivum L.) Varieties
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    Scientia Agricultura Sinica. 2005, 38(05):  874-881 . 
    Abstract ( 1160 )   PDF (412KB) ( 832 )   Save
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    To explore the feasibility of improving wheat quality by genetic engineering, three Chinese winter common wheat varieties Een1, Emai11 and Emai12, were transformed with high-molecular-weight glutenin subunit (HWM-GS) genes 1Ax1 and 1Dx5+1Dy10 by particle bombardment, respectively. A significant negative correlation between plant regeneration frequency and the age of immature embryo were observed in all three tested varieties. The best transformation frequencies were achieved up to 4.5%, 2.9% and 2% for three varieties of Een 1, Emai 11 and Emai 12, respectively, when using immature embryos from young donor plants developing 12 to 14 days after blossoming. The phenotypically normal fertile T0 transgenic wheat plants of HMW-GS genes 1Dx5+1Dy10 and the endosperm-specific expressed T1 transgenic seeds were produced. Factors including explants at an optimal developmental stage from donor plant and optimal conditions of bombardment and tissue culture were proved to be the key parameters in the successful transformation procedure.
    Consensus Map of the QTL Relevant to Drought Tolerance of Maize Under Drought Conditions
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    Scientia Agricultura Sinica. 2005, 38(05):  882-890 . 
    Abstract ( 1302 )   PDF (600KB) ( 795 )   Save
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    Discovery of drought tolerant genes and their closely linked markers could provide useful information for setting molecular breeding strategy. In this paper, QTL relevant to drought tolerance in maize genomic database were explored, and the consensus map was constructed with the aid of high-density linkage map IBM and the adjacent markers to each QTL. Consequently, a total of 181 QTL involving in the expression of 9 morhpho-physiological traits in 10 mapping populations of maize under drought conditions were screened out. The length of the consensus map constructed was 3582.1cM. Furthermore, 15 "universal drought tolerance QTL" and their linked markers were identified. The results will facilitate to determine the genetic loci and molecular marker-assisted selection strategy for drought tolerance in maize.
    Cloning of fae1 Gene Partial Sequence and SNP Analysis in Brassica Species
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    Scientia Agricultura Sinica. 2005, 38(05):  891-876 . 
    Abstract ( 1342 )   PDF (336KB) ( 714 )   Save
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    fae1 gene is a rate-limiting gene for erucic acid synthesis in Brassica. Partial sequences of the gene were cloned by polymerase chain reaction (PCR) strategy with primers F1 and R1 designed according to the DNA sequence of fae1 reported in Genbank (AF490462). Twelve cultivars with different erucic acid content from B.napus, B.oleracea and B.rapa , including 2 resynthesized B.napus lines were used as genomic DNA source materials. The PCR products were cloned into the vector pGEM-T and sequenced individually. All the 12 cultivars showed similar nucleotide sequences with a same 1007bp in length. A clustal alignment of the 12 sequences showed that fae1 gene is highly conservative among different species tested, and within the 1007bp region showed 23 sites of single nucleotide polymorphism corresponding to 11 amino acid changes at protein level. No significant difference was found between natural and resynthesized lines of B.napus. Specific SNP sites were found to distinguish fae1 genes between high and low erucic acid and between C genome and A genome.
    TILLAGE & CULTIVATION·PHYSIOLOGY & ECOLOGY
    Activities of the Enzymes Involved in Starch Synthesis and Starch Accumulation in Grains of Wheat Cultivars GC8901 and SN1391
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    Scientia Agricultura Sinica. 2005, 38(05):  897-903 . 
    Abstract ( 1124 )   PDF (356KB) ( 741 )   Save
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    Two wheat cultivars, GC8901 (hard winter wheat) and SN1391 (soft winter wheat), were used for investigating the changes of enzyme activities for sucrose metabolism and starch biosynthesis and the accumulation characters of starch composition. Result showed that, the activities of sucrose (SS), sucrose-phosphate synthase (SPS), adenosine diphosphorate glucose pyrophrylase (ADPGPPase) and soluble starch syntheses (SSS) of SN 1391, which has more starch, were significantly higher than GS 8901, that with low starch content. But the changes of granule-bound starch synthase (GBSS) activity were consistent with the amylose content which indicated that amylose contents in grain were determined by GBSS activity, especially the activity at later grain filling stages. Simulating with Richards Equation showed that it was initiating time and accumulation rate, but not accumulation duration that determined the content of starch composition. Furthermore, the changes of sucrose transport capacity were consistent with SSS and GBSS activities, starch accumulation rate was accordant to ADPGPPase and SS/SPS ration, not SS, SPS, SSS or GBSS activities. The results suggested that there is no inevitable relation of starch accumulation rate and starch composition contents with the activity of single enzyme such as SS, SPS, SSS or GBSS, but closely related to ADPGPPase activity and SS/SPS ratio, and it was SPS and ADPGPPase have played a vital role in the biosynthetic pathway. Later polymerization reactions catalyzed by SSS and GBSS do not seem to control the rate of starch accumulation, but do affect starch structure.
    Effect of the Composite Root Population of Wheat-Cotton Double Cropping System on Soil Enzyme Activity and Soil Nutrient Content at the Cotton Rhizosphere and Non-Rhizosphere Zones
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    Scientia Agricultura Sinica. 2005, 38(05):  904-910 . 
    Abstract ( 1054 )   PDF (378KB) ( 860 )   Save
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    Under the conditions of high yielding cotton-wheat double cropping system, the effect of the composite root population of wheat-cotton double cropping system on soil enzyme activity and soil nutrient content at cotton rhizosphere and non-rhizosphere zones was studied in 3 treatments, i.e. wheat-cotton double cropping system in which the roots of wheat and cotton mixed naturally (WCRM), wheat-cotton double cropping system in which the roots of wheat and cotton separated with nylon net (WCRN),and wheat-cotton double cropping system in which the roots of wheat and cotton separated with plastic film(WCRP). The results showed that: in the symbiotic period of wheat and cotton, compared with WCRP, the activity of urease, invertase and protease at cotton rhizosphere and non-rhizosphere soil increased in WCRM and WCRN, and the soil enzyme activity in WCRN was the highest one. The soil nutrient content at cotton rhizosphere and non-rhizosphere zones reduced in WCRM, and the soil nutrient content in WCRN was the highest one as a result of yarn net slowed down the nutrient competition of wheat root to cotton root and secretion from wheat root's enhancement at the same time. In full-bud stage, wheat had been harvested, the soil enzyme activity and soil nutrient content at cotton rhizosphere and non-rhizosphere zones improved in different degrees in the composite root population, and WCRM improved the most, it's urease and invertase activity was the highest, and the soil organic matter content and total nitrogen content were higher than WCRP, but lower than WCRN. In wheat-cotton double cropping system, there were not only disadvantage nutrition competition between wheat and cotton at underground part, but also advantage part in which the soil enzyme activity and soil nutrient content at cotton rhizosphere and non-rhizosphere zones improved.
    Studies on Wheat Canopy Structure by Hyperspectral Remote Sensing
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    Scientia Agricultura Sinica. 2005, 38(05):  911-915 . 
    Abstract ( 1179 )   PDF (302KB) ( 798 )   Save
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    Some wheat varieties were selected in this experiment, and different density treatments were arranged. The results were as follows: 1) Leaf Orientation Value (LOV) and Leaf Area Index (LAI) of wheat had different contributions to canopy spectra, for example, LOV affected greatly canopy spectra more than LAI in jointing stage, but LAI had more effects on spectra after the ground was near to be covered completely. 2) 20 treatments including different varieties and densities were arranged in this experiment, the result of cluster analysis showed that all these treatments can be parted into four clusters: A-erect type and low LAI, B-erect type and high LAI, C-horizontal type and low LAI, D-horizontal type and high LAI. Their spectral reflectance had obvious difference in 400-700 nm and 700-1 150 nm at jointing, which indicated that jointing stage was an important stage in plant type recognition. 3) There was an obvious distribution difference among different clusters in scatter plot (X=△R890, Y=R890), △R890 was the reflectance dispersion of booting stage and jointing stage. It was seen from the Y-axis direction that R890 of a horizontal type variety was higher than erect type ones, and seen from the X-axis direction, the greater △R890 was the lower population one within the same type varieties, which indicted that the combination of plant-type and the population magnitude can be identified qualitatively by this method.
    Crude Protein Content and Yield of Fifty Maize (Zea mays L.) Varieties Widely Planted in the North of China
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    Scientia Agricultura Sinica. 2005, 38(05):  916-921 . 
    Abstract ( 1253 )   PDF (435KB) ( 956 )   Save
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    In field experiments,crude protein content (CPC)and yield of fifty maize varieties widely planted in the north of China since 1990s were studied. By exploring analysis and testing of normality for stalk CPC,gain CPC,and whole-plant CP yield (CPY),each of which accorded with normal distribution N(5.48,0.532),N(9.42,0.792),and N(530.67,64.632),respectively. The ranges of stalk CPC were 4.38%-7.09%,grain CPC were 7.03%-11.00%,whole-plant CPY were 417.43-726.72 g/plant. The stalk CPC was not significantly correlated with grain CPC (P>0.05). Through hierarchical cluster analysis for stalk CPC and grain CPC of the fifty maize varieties,the maize varieties could be classified into 3 types:high-grain CPC with low-stalk CPC type(HGLS),low- grain CPC with low-stalk CPC type(LGLS),and high-grain CPC with high-stalk CPC type(HGHS). Currently, maize varieties in the north of China were short of HGHS type hybrids which was only 20 percent,it is suggested that breeding and selection for this type of maize should be strengthened.
    Effects of Water Stress on the Protective Enzyme Activities and Lipid Peroxidation in Roots and Leaves of Summer Maize
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    Scientia Agricultura Sinica. 2005, 38(05):  922-928 . 
    Abstract ( 1060 )   PDF (408KB) ( 1069 )   Save
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    Using water proof installations, a systematic study was conducted determine the effects of water stress on the protective enzyme activities and lipid peroxidation were determined. The results showed that: Under water stress, the activities of superoxide dismutase(SOD), catalase(CTA),and peoxidase(POD) in leaves and roots increased sharply at prophase and metaphase growth stages such as male tetrad stage, while declined at the evening developmental stages such as milky stage.The protective enzyme activities in root are lower than that in leaves. The content of malondialdehyde (MDA) increased following the stronger water stress, and also the content of MDA in roots was lower than that in leaves. The protective enzyme activities and lipid peroxidation in root were positively related to that in leaf, and furthermore the most of correlated coefficients showed the significant difference. In addition, the soluble protein in roots and leaves decreased. The ear characteristics were deteriorated and the economic yields of maize decreased significantly under water stress. The main factors that caused reduction of yields were the decrease of ear kernel number and 100-kernel weight.
    PLANT PROTECTION
    Cloning and Sequence Analysis of Diverse Members of avrBs3/PthA Family of Xanthomonas oryzae pv.oryzicola
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    Scientia Agricultura Sinica. 2005, 38(05):  929-935 . 
    Abstract ( 1118 )   PDF (406KB) ( 979 )   Save
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    Xanthomonas oryzae pv.oryzicola (Xooc) and X.o.pv.oryzae (Xoo) are two pathovars on rice. An EcoRI-BamHI DNA fragment, corresponding to the coding region NLS and AD of AvrXa3 of Xoo, was used as a probe to screen the genomic library of Xooc. Twenty three avrBs3/PthA gene clones were obtained after positive clones of the library were digested by EcoRI endoenzyme and proved by Southern blot with the probe. Sequencing randomly selected clones, avr/Pth13 and avr/pth14, indicated that they were the members of avrBs3/PthA family of Xanthomonas pathogenic bacteria. The two putative proteins were similar as other AvrBs3/PthA, possessing the same 5′- and 3′- terminals, one leucine zipper (LZ), three nuclear localization signals (NLSs) and one acidic transcriptional activation domain (AD). However, the difference of the gene with other avrBs3/PthA family members was the repeat numbers of 34-amino-acid unit coded by 102 bp repeat. The repeat numbers of 102 bp unit was 5.5 in avr/Pth13 gene and 19.5 in avr/Pth14。Alignments of 12-13th residues in 34-amino-acid unit of Avr/Pth13 and Avr/Pth14 with those of other reported AvrBs3/PthA proteins demonstrated hypothetically that at least three HD residues in 12-13th position of 34-amino-acid unit were necessary for avirulence or/and virulence of the proteins. Pathogenicity of strain PXO99A containing avr/Pth13 and avr/Pth14 gene, respectively, on near isogenic lines (NILs) and rice cultivars with Xoo R genes showed that the virulence of PXO99A was strengthened by avr/Pth13 gene, and reduced by avr/Pth14. All the results confirmed that diverse avrBs3/PthA genes existed in Xooc as that in Xoo determining virulence in bacterial streak and blight diseases of rice. It is the first report that the avrBs3/PthA genes were cloned from Xooc and avr/Pth13 is the smallest gene in the avrBs3/PthA family up to now。
    Molecular Divergence Estimation of Chinese Upland Cottons with Fusarium and Verticillium Wilt Resistance
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    Scientia Agricultura Sinica. 2005, 38(05):  936-943 . 
    Abstract ( 1227 )   PDF (373KB) ( 852 )   Save
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    SSR(simple sequence repeats) and AFLP(amplified fragment length polymorphism) were used to assess the genetic divergence of 54 Chinese cottons with Verticillium and Fusarium wilt resistance. 446 DNA polymorphic markers were scored from amplifications of 22 SSR primers and 20 AFLP primer pairs, with 8.26 markers for each variety in average. Euclidean distances were calculated using the software of SPSS version 11.5 and dendrograms were constructed. The Euclidean distances were 5.568-11.790, with the mean Euclidean distance 8.906. Average genetic distances for every single variety were 8.530-10.011, and those of 10 varieties, accounted for 18.52% of the total, were above the total mean Euclidean distance. The genetic distances of 85.55% of the varieties ranged from 7.000 to 10.000. All of above results showed a narrow genetic base in the tested cotton population. Four foreign cultivars were used as control and their Euclidean distances varied from 6.481 to 12.490. The Euclidean distances between Chinese- and foreign- cultivars were 6.481-12.490. Euclidean distances of 82.02% of the varieties distributed from 8.000 to11.000. These indicated that the genetic divergence between the Chinese cottons and foreign cultivars was more abundant. All the determined materials could be classified into five SAGs (SSR&AFLP-based groups) . The SAGs, to some extent, could reflect the pedigrees of the cultivars.
    Studies on Inheritance of Symptom Reaction to Soybean Mosaic Virus in Soybean
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    Scientia Agricultura Sinica. 2005, 38(05):  944-949 . 
    Abstract ( 810 )   PDF (270KB) ( 755 )   Save
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    Five kinds of crosses (M×R, M×N, N×R, N×N and M×M,R, N and M representing resistant, necrotic and mosaic symptom, respectively) and their parents were used to study the inheritance of symptom reaction to soybean mosaic virus (SMV) in soybean. The results were as follows: (1) All plants of F1 from M×R crosses were symptomless. F2 populations segregated in a 3R: 1S ratio. The results showed that a single dominant gene controls resistance to SC8. (2) All F1 plants from M×N produced necrotic symptom, F2 population segregated in a 3N:1S ratio. It indicated that genes conditioning necrotic and mosaic symptom were at a same locus with necrosis being dominant to mosaic. (3) All F1 plants from N×R performed symptomless, F2 population segregated in a 3R : 1N ratio. It indicated that genes conditioning resistant and necrotic reaction are at a same locus with resistance being dominant to necrosis.(4)Non-segregation in N×N and M×M crosses showed that the genes for necrosis in different cultivars were at a same locus and the genes for mosaic symptom in different cultivars were also at a same locus. It was inferred that three types of symptoms of SMV in soybean were conditioned by a set of multiple alleles and the gene symbols SR, sN and sm were assigned for resistance, necrosis and mosaic symptom, respectively, with SR dominant to sN and sm and sN dominant to sm. Key words: Soybean mosaic virus (SMV); Symptom; Inheritance; Multiple alleles
    Control of Postharvest Diseases of Tomato Fruit by Combining Antagonistic Yeast with Sodium Bicarbonate
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    Scientia Agricultura Sinica. 2005, 38(05):  950-955 . 
    Abstract ( 1701 )   PDF (368KB) ( 900 )   Save
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    Biocontrol efficacy of antagonistic yeast Crytococcus laurentii in combination with different concentrations of sodium bicarbonate (SBC) against postharvest diseases caused by Botrytis cinerea and Pythium aphanidermatum in tomato fruit were investigated. The results indicated that combining C. laurentii with SBC provided a more effective control on B. cinerea and P. aphanidermatum than applying the antagonistic yeast or SBC alone, and C. laurentii combined with 5% SBC showed the best effect. Growth of B. cinerea and P. aphanidermatum were inhibited on PDA containing different concentrations of SBC and the inhibitory effect on B. cinerea was stronger than on P. aphanidermatum. Growth of C. laurentii was inhibited by SBC both in the wound of tomato fruit and NYDB broth.
    SOIL & FERTILIZER·WATER-SAVING IRRIGATION
    Modeling and Applications of Soil Organic Matter in Intensive Cropping in China's Huang-Huai-Hai Plain
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    Scientia Agricultura Sinica. 2005, 38(05):  956-964 . 
    Abstract ( 1249 )   PDF (481KB) ( 732 )   Save
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    Organic carbon dynamics in intensive agricultural soils is significant for sustainable crop production. Based on the short-, mid-term buried organic material experiments in China's Huang-Huai-Hai Plain, the CQESTR model is modified with the division of 4 decomposition stages according to the relation between biomass decomposition and cumulative degree days and the biomass type factors in different stages are determined. Through the validation of soil organic matter (OM) by 7 independent long-term soil fertility and fertilizer efficiency experiments in China's Huang-Huai-Hai Plain, the modified CQESTR model can be used to predict the mineralization of residue, added organic amendment and native soil OM based on cropping and management practices, thus predicting the effect of tillage and crop rotation on organic carbon decomposition and storage in agricultural soils. The modified model provides estimates with a 95% confidence interval of 1.91 g·kg-1 OM and regression coefficient r2 of 0.91 on 1 151 pairs of fitted and observed soil OM from 7 independent fertility experiments in the plough layer. The modified model performs better than the original one.
    The Interspecific Nitrogen Facilitation and the Subsequent Nitrogen Transfer Between the Intercropped Wheat and Fababean
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    Scientia Agricultura Sinica. 2005, 38(05):  965-973 . 
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    A pot experiment was carried out to investigate the interspecific nitrogen facilitation between the intercropped wheat and fababean. The results from the isotope dilution method showed that wheat competed more fertilizer N than fababean, and fababean depended more on biological nitrogen fixation. 15N recovery rate of wheat with solid barrier, mesh barrier and no barrier was 58%, 73% and 52%, respectively. Nevertheless, 15N recovery rate of fababean was 30%, 20% and 3%, respectively. The results in the mesh barrier also showed that due to competition from wheat for the soil available N, the percentage of fixed N in fababean was enhanced. The percentage of N absorbed by fababean from pots with solid barrier, mesh barrier and no barrier was 58%, 80% and 91%, respectively. Therefore, the complementary N use did exist in wheat-fababean intercropping, and both the interspecific nitrogen competition and facilitation occurred in wheat-fababean intercropping. At last but not the least, the isotope dilution method showed nitrogen transfer occurred from fababean to the associate wheat, and 5% of total N of fababean was transferred to wheat.
    Analysis on Supply and Demand of Water Resources and Evaluation of the Security of Water Resources in Irrigation Region of the Middle Reaches of Heihe River , Northwest China
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    Scientia Agricultura Sinica. 2005, 38(05):  974-982 . 
    Abstract ( 1022 )   PDF (454KB) ( 1011 )   Save
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    The model of supply and demand of water resources was established, combined with meteorological, hydrological, soil condition, planting industry structure, vegetation and economic development of irrigation region in the middle reaches of Heihe river, northwest China to develop a calculation and evaluation model of regional supply and demand of water resources. After unified management of water resources in Heihe river basin between Gansu Province and Inner Mongolia in China, the amount of surface water from Heihe river decreased and that of groundwater increased, the volume of groundwater mining is increasing and the groundwater table is rising in studied regions now. The groundwater exploitation potential of irrigation region is less than the permissible utilization volume of groundwater, groundwater overdraft is remarkable in irrigation region. This paper has also proposed the measures to meet water security in the middle stream and related suggestion has been raised to guarantee this balance. It is found that water supply and water demand was safe in 1997, but water supply was not safe in 2003. If the water resources management, surface water and groundwater planning, the patterns and techniques of optimum utilization of water and land in this region would be tackled, the problem of the security of supply and demand of water resource and eco-environment is confronted with the baptism of Heihe river basin.
    The Concept of Agricultural Productivity on Ecosystem Scale and Its Measurement
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    Scientia Agricultura Sinica. 2005, 38(05):  983-989 . 
    Abstract ( 966 )   PDF (261KB) ( 768 )   Save
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    What is the connotation and the extension of the productivity concept and how to calculate it are the core problems in evaluating an agricultural production process. The attributes of agricultural production processes have changed greatly in the last 100 years, and the methods to describe and measure the productivity failed to keep up with, resulting in a totally vague, incomplete valuation of the production processes. Sometimes the serious pollution is missed behind shortdated money making, bringing along a blooming and flooding polluting; sometimes the great ecological benefits are ignored and the corresponding production processes are died from "economic deficit". With the old agricultural productivity indices, it can't be told what an agroecosytem is ultimately producing to mankind, pollution or welfare. A new concept, Agricultural Productivity on Ecosystem Scale(APES) and the related indices are defined here to solve these problems. Emergy and exergy are introduced to help the measurement of the indices. Emergy is the memory of energy, can be used to measure the total cost, including natural capital and social capital. APES of the agroecosystem is the aggregated contributions of all components to mankind and its habitat in uniformed dimensions, may be expressed in material, energy, information, organization or pollution, damage etc., positive or negative, are calculated based on exergy methods. And a related index Agroecological Coupling Degree(ACD)shows sustainability.
    Approach to Health Diagnoses of Eco-Economic System in Mesoscale in Loess Hilly Area
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    Scientia Agricultura Sinica. 2005, 38(05):  990-998 . 
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    Ecosystem health is the aim of soil conservation and ecosystem rehabilitation on the Loess Plateau. In this paper, based on the analysis of the ecosystem health situation and its diagnoses, a set of indictors of eco-economic system in mesoscale was established in loess hilly area. The mean square deviation was used to calculate the weight, and compositive appraisement method of hierarchy multiplayer and the linear weighted function were introduced to build the two levels and integrated indicator of health diagnoses model - the health index. The dynamics of health indicators for its time and space of changes were also analyzed. The results showed that under different re-habilitation practices the watershed ecosystem health had improved from 0.2125 to 0.5188, and were able to develop sustainably. By analysis of limiting degree and the advantage degree, different management approaches were suggested in improving the health situation which provided a scientific base for restoration of watershed ecosystem in this region.
    HORTICULTURE
    Combined Effects of High Level of Mn and Low pH on Oxidative Stress and Antioxidant Enzymes in Cucumber Roots
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    Scientia Agricultura Sinica. 2005, 38(05):  999-1004 . 
    Abstract ( 1124 )   PDF (403KB) ( 967 )   Save
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    Effects of high level of Mn on oxidative stress and antioxidant enzymes in cucumber roots under different pH (pH 4.5 and pH 6.5) were studied. The results indicated that lipid peroxidation increased seriously with the increasing concentration of Mn, especially under low pH. As a results, the growth of cucumber roots and shoots was significantly inhibited. High level of Mn led to an elevation in activities of Mn-SOD and Fe-SOD, which played an important role in removing O2?· CAT was sensitive to high level of Mn, and its activity significantly decreased under high level of Mn and lower pH,while the activities of GPX, APX, DHAR and GR increased under low pH and some extents of high level of Mn, which indicate their important role in scavenging reactive oxygen in cucumber roots tolerance to low pH and high level of Mn.
    Screening on Chilling Tolerance of Different Eggplant Rootstock Seedlings
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    Scientia Agricultura Sinica. 2005, 38(05):  1005-1010 . 
    Abstract ( 1119 )   PDF (420KB) ( 798 )   Save
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    The changes of morphology, physiology and biochemistry of eggplant rootstock seedlings were studied under low temperature stress, with Jinong 2000 as control. The results showed that these materials tested were sensitive to low temperature stress in the primary period of treatment. For instance, such indexes as chilling injury index, leaf electrolytic leakage, MDA content and SOD activity improved fast, and such indexes as POD activity, CAT activity, AQY, photosynthetic rate, Fv/Fm and ФPSⅡ decreased quickly. Subsequently, leaf protective enzyme activity, photosynthetic rate and Fv/Fm increased gradually. With the prolonging time of low temperature stress, leaf protective enzyme activities and photosynthetic rate decreased, but the degree of decrease of Torvum and Hiranasu were slight and their activities still kept at a higher level. The result of comprehensive screening showed that the chilling tolerance of Torvum and Hiranasu was better, Jinong 2000 was the lowest, Daidaro and Taibyo VF were in the middle in this study.
    ffects of Boron in Soil on Physiological-Biochemical Characteristics and the Yield and Quality in Garlic
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    Scientia Agricultura Sinica. 2005, 38(05):  1011-1016 . 
    Abstract ( 1054 )   PDF (342KB) ( 710 )   Save
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    The effects of boron on physiological-biochemical indexes and the yield and quality in garlic (Allium sativum L.)were studied. The results showed that the activity of POD and SOD, soluble protein content, photosynthetic pigment content and photosynthetic parameter in garlic(Allium sativum L.)leaf all reached maximum while boron in soil was 0.85 ?g·g-1. Meanwhile , the average weight of single bulb and bolt was 23.58% and 31.31% higher than that of CK, respectively.The activity of NR and CAT increased with the increase of concentration of boron in soil. The content of allicin, soluble protein and Vc in bulb increased, respectively, by 30.84%, 33.28%, and 232.22% when boron in soil was 1.29 ?g·g-1. And on this concentration, the content of free amino acid and soluble protein in bolt improved by 20.79% and 13.82%, respectively. The content of soluble sugar and Vc in bolt were the highest when boron in soil was 0.62 ?g·g-1.
    Phylogenetic Analysis of Cultivated Radish (Raphanus sativus L.) Germplasm with AFLP
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    Scientia Agricultura Sinica. 2005, 38(05):  1017-1023 . 
    Abstract ( 915 )   PDF (250KB) ( 995 )   Save
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    Phylogenesis of 56 cultivated radish germplasm from different countries or regions was analyzed with amplified fragment length polymorphism (AFLP). A total of 327 bands were detected with 8 pairs of informative AFLP primers among the accessions tested. Of them, 128 bands (39.1%) were polymorphic, indicating abundant genetic diversity existing among the cultivated radish. The 56 cultivars were clustered into 5 groups and 9 subgroups by cluster analysis, and 4 groups and 7 subgroups by principal coordinate analysis. The two methods above exhibited similar phylogenesis among the tested cultivars. The results from molecular classification were almost coincided with germplasm phenotypes.
    Physiological Reaction on Resistance to Iron Deficiency in Transgenic Lines of Malus robusta by Introducing LeIRT2 Gene
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    Scientia Agricultura Sinica. 2005, 38(05):  1024-1028 . 
    Abstract ( 1245 )   PDF (254KB) ( 776 )   Save
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    Two transgenic plants of Malus robusta were hydroponically planted to test their efficiency to iron-deficiency(iron concentration 10-6mol·L-1), and the tolerance was found in these transgenic plants. Their leaf chlorophyll content and net photosynthetic rate were much higher than those of control, which showed severe chlorosis under the same condition. The rate of root H+ excretion and ferric-chelate reductase activity increased in transgenic lines and substantially higher than those of control. Scanning photos of root absorption region and root hair by electronic microscope in transgenic lines and non-transgenic plants were different under same iron treatment.
    ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT
    Effects of Chemical Activation on the Parthenogenetic Development of Porcine in vitro Maturation Oocyte
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    Scientia Agricultura Sinica. 2005, 38(05):  1029-1033 . 
    Abstract ( 1060 )   PDF (216KB) ( 698 )   Save
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    The objective of this study was to determine the effects of ionomycin combined with cytochalasin B (CB), cycloheximide (CHX), or 6-dimethylaminopurine (6-DMAP) on the activation of porcine oocytes. In experiment 1, in vitro matured oocytes were activated with ionomycin at 15, 20, 25 or 30 mol·L-1. Activation rates of 20, 25 mol·L-1 and 30 mol·L-1 treatments were higher (P<0.05)than that of 15 mol·L-1 treatment. In experiment 2, in vitro matured oocytes were activated with 20 mol·L-1 ionomycin at 10,20,30,40 or 50 min and then incubated with 2 mmol·L-1 6-DMAP 6 h.Cleavage rates and blastocyst formation rates after 40 min treatments [(72.40±13.02)%, (25.37±11.43)%]was higher (P>0.05) than that of the either treatments. In experiment 3, matured oocytes were activated with ionomycin and then incubated with 7.5 g·ml-1 CB,10 g·ml-1 CHX, 2 mmol·L-1 6-DMAP, 7.5 g·ml-1 CB +10 g·ml-1 CHX or 7.5 g·ml-1 CB +2 mmol·L-1 6-DMAP for 6 h. Activation rates, cleavage rates and blastocyst formation rates of 2 mmol·L-1 6-DMAP treatment [(86.05±4.29)%, (61.77±8.10)% and (21.62±3.31)%] was higher (P<0.05) than that of 7.5 g·ml-1 CB treatment. In experiment 4, matured oocytes were activated with ionomycin and then incubated with 2 mmol·L-1 6-DMAP for 3.5, 5.5 or 7.5 h. Cleavage rates and blastocyst formation rates of 5.5 h treatment [(66.59±14.36)% and (25.40±10.16)%] was higher (P>0.05) than that of other treatments. In conclusion, activation of porcine oocytes appears to be most successful using the combination of ionomycin (20 mol·L-1, 40 min) followed by 6-DMAP (2 mmol·L-1 , 5.5 h).
    In vitro Expression and Bioactivity of Chicken Interleukin-2
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    Scientia Agricultura Sinica. 2005, 38(05):  1034-1039 . 
    Abstract ( 1303 )   PDF (400KB) ( 864 )   Save
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    In this study, the recombinant chicken interleukin-2 (rchIL-2) was successfully produced in the pBAD/His B expression system induced by L-(+)-arabinose. By SDS-PAGE analysis, the observed molecular weight of rchIL-2 fusion protein without the signal peptide was 18 kD. Monoclonal and polyclonal antibodies specific for rchIL-2 were produced. These antibodies were used to develop a mAb-based antigen capture ELISA (AC-ELISA). Based on the constructed AC-ELISA, in vitro kinetics of endogenous chicken IL-2 production were detected. The soluble rchIL-2 protein purified under native conditions was shown to activate the proliferation of chicken lymphocytes stimulated by Con A for 24 h in a dose-dependent manner. This activity cannot be neutralized by mAbs to chIL-2, but can be neutralized by anti-chIL-2 polyclonal antibody. These results demonstrated that the E.coli-derived rchIL-2 and polyclonal antibody to chIL-2 have bioactivity.
    Profiling of Gender-Specific Gene Expression for Ascaris suum by Suppression Subtractive Hybridization
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    Scientia Agricultura Sinica. 2005, 38(05):  1040-1045 . 
    Abstract ( 878 )   PDF (262KB) ( 860 )   Save
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    Attempting a new method of gender-controlling nematode infection in animal production, differentially expressed male and female cDNA libraries of the adult Ascaris suum were constructed using the technique of suppression subtractive hybridization (SSH). The gender specificity of the constructed archives was determined using Southern blots with Digoxigenin(DIG)- labeled male and female cDNA probes. Twenty-five clones from each archive were selected ad libitum for sequence determination and homology analysis by BLAST online. Twenty of the 25 ESTs from the male cDNA library have a homology to the genes from C. elegans and 11 of the 19 ESTs from the female cDNA library have a homology to the genes from C. elegans. The successful construction of differentially expressed male and female cDNA libraries have provided a foundation for further study of the gender-specific genes.
    Construction of Expression Vectors for Detection of β Estradiol
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    Scientia Agricultura Sinica. 2005, 38(05):  1046-1051 . 
    Abstract ( 996 )   PDF (375KB) ( 1116 )   Save
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    The estrogenic endocrine disrupting compounds whether from environment and food chains or from chemicals illegally used in animal production pose a serious threat to the safety of animal derived products. Because of the huge diversities of these chemical structures, it is necessary to establish high-throughput screening and detecting methods to screen them. In this study, 4 recombination expression vectors with enhanced GFP as reporter gene were constructed. These constructs, namely, pERE-SV-EGFP, pERE-TA-EGFP, pERE-CMV-EGFP, pERE-TK-EGFP, only differ in promoters. Then the four expression vectors were transiently transfected into MCF-7 cells with liposome transfection reagent Tfx-20. The constitutive expressions of EGFP in transient transfected cells were related to the activity of the promoters in the expression vectors. And the fluorescence intensity treated by 17- βestradiol (10-9 mol·L-1) was two-fold higher than that treated by DMSO (control) in cells transiently transfected with pERE-SV-EGFP, pERE-TA-EGFP and pERE-TK-EGFP. These results indicated that the expression of reporter gene in ERE-SV-EGFP, pERE-TA-EGFP and pERE-TK-EGFP was estrogenic chemical dependent, and could be used for stable transfection to establish stable cell lines for estogenics screen.
    A Comparison of the Protein Composition of the Middle Silk Gland Cells of the Fifth Instar Larvae of Silkworm
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    Scientia Agricultura Sinica. 2005, 38(05):  1052-1058 . 
    Abstract ( 970 )   PDF (331KB) ( 925 )   Save
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    Research of the protein composition of different parts of the middle silk gland (MSG) cells of the fifth instar larvae of silkworm (Bombyx mori L.)are useful to find the functional protein concerned with the composition and secretion of the sericin protein. Using the protein two dimensional electrophoresis (2D-PAGE) and the image analysis technology, a significant differences of the protein composition among the anterior, middle and posterior parts of the MSG cells of silkworm were found. Twenty specific proteins that expressed only in anterior part of the MSG cells were likely to relate with the composition and secretion of 4 kinds of sericin proteins, which are Ser2A, Ser2B, S4 and S5. Twenty-two specific proteins that expressed only in middle part of the MSG cells were likely to relate with the composition and secretion of 4 kinds of sericin proteins, which are Ser1B, Ser1C, Ser1D and S3. Twenty-seven specific proteins that expressed only in posterior part of the MSG cells were likely to relate with the composition and secretion of 2 kinds of sericin proteins, which are Ser1A and S3. Moreover, fifty-one specific proteins which expressed in middle and posterior, but not in anterior part of MSG cells and twenty proteins whose volume in middle and posterior part of MSG cells were higher than that in anterior part likely participated in the synthesis of the sericin protein of the corresponding parts.
    RESEARCH NOTES
    Effects of Eco-Physiological Factors on Storage Protein 11S/7S Ratio in Soybean Seeds
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    Scientia Agricultura Sinica. 2005, 38(05):  1059-1064 . 
    Abstract ( 843 )   PDF (263KB) ( 670 )   Save
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    The soybean cultivar Yudou No.25 was sown at different dates in 5 locations of Henan Province in 2001 and 2002. One hundred and nine samples of 11S/7S ratio in soybean seeds and 29 eco-physiological factors were received. The stepwise regression was used to screen the correlated factors,which significantly affected 11S/7S ratio. Results showed that: (1) The sunshine hour at seed-filling and maturation showed in curve relation to 11S/7S ratio, and optimum value for getting higher and lower 11S/7S ratio were 459.2 h and 110.8 h, respectively. The lower diurnal temperature variation at seed-filling and maturation was favorable for getting higher 11S/7S ratio. Lower diurnal temperature variation and higher mean temperature at seedling brought on higher 11S/7S ratio, both of the two factors showed liner relationship with the ratio of 11S/7S. Higher diurnal temperature variation at branching was suitable for higher 11S/7S ratio. (2) Liner relationship also existed between 11S/7S ratio and soil K content, the maximum and minimum 11S/7S ratio emerged when soil K content were 0.83% and 1.32%, respectively. (3) The 11S/7S content would not be affected by other eco-physiological factors in the range of this test.
    Analysis of Body Conformation and Fitting Growth Model in Tibetan Chicken Raised in Plain
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    Scientia Agricultura Sinica. 2005, 38(05):  1065-1068 . 
    Abstract ( 818 )   PDF (216KB) ( 798 )   Save
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    The altiplano breed, Tibetan chicken which are raised in Beijing was studied on the body conformation and growth rhythm, and the two curves of Logistic and Gompertz model were used to fit the growth model. The results indicate that cocks and hens raised in plain grow basically unanimous before the age of 4 weeks, but the cock grows faster than the hens obviously after age of 4 weeks. The fitting results of two kinds of models are very good, especially Gompertz model is better. The growth inflexion of the cock and hen are the age of 75.69 days, and 61.91 days respectively. The adult live weight of the cock is higher than the hen notably, but the day of inflexion is late to the hen relatively.
    In vitro Adsorption of Aflatoxin Adsorbing Nano-Additive for Aflatoxin B1, B2, G1, G2
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    Scientia Agricultura Sinica. 2005, 38(05):  1069-1072 . 
    Abstract ( 1116 )   PDF (307KB) ( 693 )   Save
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    A new aflatoxin adsorbing nano-additive (AAN) was configured by modifying montmorillonite. The influence of shaking time, AAN amount, solution pH and equilibrium temperature on the adsorption of AAN for aflatoxins was studied and the optimal experiment term for adsorption was determined. The results showed that the adsorption of AAN for aflatoxins was strong and quick, the adsorption reached equilibrium after 60 min, the desorption rate of aflatoxins from AAN was low and less than 10%, which was a chemisorption process. These findings suggest that AAN, as a new nano-adsorbent, has promising prospects.