Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (05): 1034-1039 .doi: 10.3864/j.issn.0578-1752.at-2004-2889

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

In vitro Expression and Bioactivity of Chicken Interleukin-2

,,,,,,,   

  1. 浙江大学动物预防医学研究所病毒与免疫研究室
  • Received:2004-10-28 Revised:1900-01-01 Online:2005-05-10 Published:2005-05-10

PDF

921

Cited

21

Abstract: In this study, the recombinant chicken interleukin-2 (rchIL-2) was successfully produced in the pBAD/His B expression system induced by L-(+)-arabinose. By SDS-PAGE analysis, the observed molecular weight of rchIL-2 fusion protein without the signal peptide was 18 kD. Monoclonal and polyclonal antibodies specific for rchIL-2 were produced. These antibodies were used to develop a mAb-based antigen capture ELISA (AC-ELISA). Based on the constructed AC-ELISA, in vitro kinetics of endogenous chicken IL-2 production were detected. The soluble rchIL-2 protein purified under native conditions was shown to activate the proliferation of chicken lymphocytes stimulated by Con A for 24 h in a dose-dependent manner. This activity cannot be neutralized by mAbs to chIL-2, but can be neutralized by anti-chIL-2 polyclonal antibody. These results demonstrated that the E.coli-derived rchIL-2 and polyclonal antibody to chIL-2 have bioactivity.

Key words: Chicken, Interleukin-2, Bioactivity, Monoclonal antibody, Polyclonal antibody, Ag capture ELISA

[1] ZHAO QingYao, WANG XiaoMing, XING Tong, LI LingYun, XU XingLian, ZHAO Xue. Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Intestinal Polysaccharides from Yellow-Feathered Chickens [J]. Scientia Agricultura Sinica, 2026, 59(6): 1317-1332.
[2] YANG LiJuan, CHEN SiYu, ZHAO Wei, ZHU Ling, GUO Lei, MA LiNa, MA RuiMin, ZHANG Juan. Whole-Genome Resequencing Reveals the Genetic Mechanisms Underlying Feather Coloration in Jingyuan Chicken [J]. Scientia Agricultura Sinica, 2026, 59(6): 1348-1360.
[3] WANG ShaoHua, FAN QiuLi, YANG JinChang, SUN YuJie, YU Niu, JIANG ShouQun. Effects of Different Levels of Mytilaria laosensis Leaves Feeding on Growth Performance, Immune Function, Antioxidant Capacity, Carcass Quality and Meat Quality of Yellow-Feathered Chickens [J]. Scientia Agricultura Sinica, 2026, 59(5): 1111-1127.
[4] LI Yun, ZHANG Fan, ZHOU YongQi, QIAO ZhiHao, LIU YanLi. Analysis of Body Size Traits During Growth and Development and Comparison of Meat Quality and Flavor Between 13 and 16 Weeks Lueyang Black-Bone Chickens [J]. Scientia Agricultura Sinica, 2026, 59(2): 427-440.
[5] ZHAO JiaLi, BIAN XianYu, SONG JiaPeng, WANG Chen, TANG XueChao, LI YunChuan, ZHOU JinZhu, ZHU XueJiao, TAO Ran, DONG HaiLong, ZHANG XueHan, LI Bin. Preparation of Monoclonal Antibody to Porcine Rotavirus VP4 and Preliminary Characterization of Antigenic Epitope [J]. Scientia Agricultura Sinica, 2026, 59(1): 220-232.
[6] HONG RunJing, ZHOU Hong, LIN HuiXing, FAN HongJie. Establishment and Application of Sandwich ELISA Method for Detecting Lawsonia intracellularis [J]. Scientia Agricultura Sinica, 2025, 58(5): 1032-1042.
[7] YAO Hong, SHI ShouRong, ZHAO RuQian. The Potential and Mechanism of Chlorogenic Acid to Alleviate Intestinal Inflammation in Chickens Based on Network Pharmacology and Molecular Docking [J]. Scientia Agricultura Sinica, 2025, 58(3): 600-616.
[8] WU ChuYan, LU HangQiong, HU MingXue, LIN YuMeng, CHEN MengYun, LIU ChangJun, LIU YongZhen, CUI HongYu, WANG SuYan, QI XiaoLe, CHEN YunTong, DUAN YuLu, GAO YuLong, ZHANG YanPing. Preparation, Epitope Identification, and Preliminary Application of Monoclonal Antibodies Against Marek's Disease Virus pp38 Protein [J]. Scientia Agricultura Sinica, 2025, 58(24): 5288-5298.
[9] HUANG HuaYun, SUI YuLe, KONG Yi, LIANG Zhong, YANG MiaoMiao, LIU Xing, HAN Wei. Regulatory Effect of FTO Gene on Lipid Deposition in Chicken Intramuscular Adipocytes [J]. Scientia Agricultura Sinica, 2025, 58(22): 4786-4796.
[10] LI XueFeng, WANG Hui, ZHANG NingBo, JIN TaiHua, ZHANG ShuEr, ZHENG QuanSheng, TAO JiaShu, LI QingKe, LÜ ShenJin, LI YongZhu. Prediction and Analysis of Feeding Density on Production Performance, Cecal Flora Diversity, Short-Chain Fatty Acid Content and Microbial Differential Function of Langya Chickens [J]. Scientia Agricultura Sinica, 2025, 58(17): 3544-3560.
[11] HUANG HuaYun, LIU Xing, WANG QianBao, LI RuiRui, YANG MiaoMiao, LI ChunMiao, WU ZhaoLin, KONG LingLin, ZHAO ZhenHua. Expression Pattern of gga-miR-30a-5p and Its Regulation of Abdominal Fat and Intramuscular Fat Deposition in Chicken [J]. Scientia Agricultura Sinica, 2025, 58(15): 3134-3144.
[12] LIU ChuanXia, CHEN Xin, WANG Xiao, LI XueWen, LI TingTing, WENG ChangJiang, ZHENG Jun. Preparation and Application of Polyclonal Antibodies Against Pig CD1d Protein [J]. Scientia Agricultura Sinica, 2024, 57(8): 1620-1628.
[13] WANG ChaoHui, ZHANG LiMin, SUN Xi, LI SiJing, YANG XiaoJun, LIU YanLi. The Model Establishment of Lipid Deposition in Primary Chicken Embryo Liver Cells Induced by Oleic Acid [J]. Scientia Agricultura Sinica, 2024, 57(23): 4806-4814.
[14] ZHANG HuiYong, WU HuCong, ZHU GuoQiang, LI GuoHui, YU Yan, YIN JianMei, XUE Qian, ZHOU ChengHao, JIANG YiXiu, SU YiJun, HUANG HuaYun, HAN Wei. Detox Dynamics and Reproductive Performance of Langya Chickens Infected with ALV-J [J]. Scientia Agricultura Sinica, 2024, 57(23): 4815-4824.
[15] MA JingE, XIONG XinWei, ZHOU Min, WU SiQi, HAN Tian, RAO YouSheng, WANG ZhangFeng, XU JiGuo. Full-Length Transcriptomic Analysis of Chicken Pituitary Reveals Candidate Genes for Testicular Trait [J]. Scientia Agricultura Sinica, 2024, 57(20): 4130-4144.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd