Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (10): 2355-2360 .doi: 10.3864/j.issn.0578-1752.at-2006-7252

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Study the expression map of salt-tolerance mutant under salt-stress in wheat using gene microarray

  

  1. 河北师范大学生命科学学院
  • Received:2006-03-01 Revised:2006-06-13 Online:2007-10-10 Published:2007-10-10

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Abstract: 【OBJECTIVE】To study the reaction of different gene of root under salt-tolerance in wheat;【METHOD】Analysed the gene expression of root in wheat using gene chip technology; 【RESULTS】 The expression of all gene in root of wheat salt-tolerance mutant RH8706-49 were analysed using gene chip technology. The responses to different time of salt stress were studied. The differentia expression map of 61,215 genes was gained. The clustering analysis of hybridizing data showed gene expression changed in root under salt stress. Both salt-induced gene and salt-inhabited gene had been found;【CONCLUSION】The mechanism of salt-tolerance in wheat is very complicated. It is the result of a lot of genes expression in line. And the function of the salt-induced gene is very important.

Key words: gene chip, wheat, salt-tolerance gene, salt stress, salt-induced gene

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