冬绿肥提升滨海盐碱土团聚性及植物源与微生物源有机碳固存

doi:10.3864/j.issn.0578-1752.2025.20.013

Abstract

Abstract:

【Objective】This study aimed to investigate the characteristics of soil aggregation and molecular origins of soil organic matter (SOM) under winter green manure cropping systems, in order to establish a scientific foundation for organic matter sequestration and soil quality improvement in saline-alkali soils. 【Method】 The field experiment, initiated in 2019 in Huanghua County of Hebei Province, involved four treatments: winter fallow (CK), Orychophragmus violaceus (OV), Triticale (TG), and winter wheat (WW). The relative contributions of SOM from different origins and related factors were elucidated. Three biomarkers - lignin phenols, glomalin-related soil protein (GRSP), and microbial necromass carbon (MNC) - were conducted to quantitatively distinguish plant- and microbial-derived organic matter components. 【Result】 Green manure generally increased soil organic carbon (SOC) storage by 12.2%-21.8% (only TG showing significant difference) and total nitrogen storage by 22.5%-36.4% compared with CK. Additionally, green manure treatments enhanced the proportion of larger macroaggregates (>2-8 mm) by 1.9-6.2 times and smaller macroaggregates (0.25-2 mm) by 12.4%-74.6%, relative to CK. The OV, TG, and WW treatments increased the mean weight diameter of aggregates by 26.7%, 86.4%, and 113.8%, respectively, compared with CK. The OV, TG, and WW also exhibited a 16.3%, 57.3%, and 86.4% higher proportion of water-stable macroaggregates (>0.25 mm) than that under CK, respectively. Moreover, the OV enhanced concentrations of vanillyl by 37.7% and cinnamyl by 149.7%, compared with CK. The OV, TG, and WW treatments increased total lignin phenols by 74.6%, 32.9%, and 38.4%, respectively, relative to CK. Besides, TG had 36.5% higher easily extractable GRSP than that under CK. TG treatment increased total MNC by 60.6% compared with CK. Again, TG had higher contributions of bacterial necromass C (19%), fungal necromass C (38%), and microbial necromass C to SOC (57%), and the contributions of microbial-derived carbon to SOC was higher than that of plant-derived across green manure treatments. 【Conclusion】 It was concluded that adoption of winter green manure increased soil aggregation and promoted the accumulation of microbial-derived carbon, potentially boosting soil quality and C sequestration in the coastal saline soils.

Key words: saline-alkali soils, green manure, microbial necromass, plant-derived lignin, aggregates, soil organic carbon sequestration

JIN XiaoYing, XIAO BingZheng, ZHANG TianJin, LIU ZhongKuan, FENG Wei, DU ZhangLiu. Winter Green Manure Enhances Soil Aggregation and Plant- and Microbial-Derived Carbon Sequestration in Coastal Saline-Alkali Soils[J].Scientia Agricultura Sinica, 2025, 58(20): 4203-4215.

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References 54

[1]	FAO. Global Status of Salt-Affected Soils-Main Report. FAO Rome, 2024.
[2]	杨劲松, 姚荣江, 王相平, 谢文萍, 张新, 朱伟, 张璐, 孙瑞娟. 中国盐渍土研究: 历程、现状与展望. 土壤学报, 2022, 59(1): 10-27.
	YANG J S, YAO R J, WANG X P, XIE W P, ZHANG X, ZHU W, ZHANG L, SUN R J. Research on salt-affected soils in China: History, status quo and prospect. Acta Pedologica Sinica, 2022, 59(1): 10-27. (in Chinese)
[3]	赵英, 王丽, 赵惠丽, 陈小兵. 滨海盐碱地改良研究现状及展望. 中国农学通报, 2022, 38(3): 67-74. doi: 10.11924/j.issn.1000-6850.casb2021-0255
	ZHAO Y, WANG L, ZHAO H L, CHEN X B. Research status and prospects of saline-alkali land amelioration in the coastal region of China. Chinese Agricultural Science Bulletin, 2022, 38(3): 67-74. (in Chinese) doi: 10.11924/j.issn.1000-6850.casb2021-0255
[4]	ABDALLA M, HASTINGS A, CHENG K, YUE Q, CHADWICK D, ESPENBERG M, TRUU J, REES R M, SMITH P. A critical review of the impacts of cover crops on nitrogen leaching, net greenhouse gas balance and crop productivity. Global Change Biology, 2019, 25(8): 2530-2543. doi: 10.1111/gcb.14644 pmid: 30955227
[5]	李可心, 王光美, 张晓冬, 张海波, 石一鸣, 季增诚, 周志勇. 毛叶苕子对滨海盐碱地土壤活性有机碳和后茬玉米产量的影响. 中国生态农业学报(中英文), 2023, 31(3): 405-416.
	LI K X, WANG G M, ZHANG X D, ZHANG H B, SHI Y M, JI Z C, ZHOU Z Y. Effect of planting and returning Vicia villosa on soil active organic carbon and yield of subsequent maize in coastal saline soils. Chinese Journal of Eco-Agriculture, 2023, 31(3): 405-416. (in Chinese)
[6]	LESTURGEZ G, POSS R, HARTMANN C, BOURDON E, NOBLE A, RATANA-ANUPAP S. Roots of Stylosanthes hamata create macropores in the compact layer of a sandy soil. Plant and Soil, 2004, 260(1): 101-109.
[7]	张京磊, 王国良, 吴波, 贾春林, 张进红, 周圆, 马冰. 滨海盐碱地苜蓿-小黑麦黑麦轮作对土壤细菌和真菌群落多样性与网络结构的影响. 生态环境学报, 2024, 33(7): 1048-1062. doi: 10.16258/j.cnki.1674-5906.2024.07.006
	ZHANG J L, WANG G L, WU B, JIA C L, ZHANG J H, ZHOU Y, MA B. The effects of alfalfa-Triticale rotation on soil bacterial and fungal community diversity and co-occurrence network in coastal saline-alkaline soil. Ecology and Environmental Sciences, 2024, 33(7): 1048-1062. (in Chinese)
[8]	王强盛, 薄雨心, 余坤龙, 刘晓雪. 绿肥还田在稻作生态系统的效应分析及研究展望. 土壤, 2021, 53(2): 243-249.
	WANG Q S, BO Y X, YU K L, LIU X X. Analysis and research prospect of effect of green manure returning on rice cropping ecosystem. Soils, 2021, 53(2): 243-249. (in Chinese)
[9]	曹卫东, 周国朋, 高嵩涓. 绿肥内源驱动土壤健康的作用与机制. 植物营养与肥料学报, 2024, 30(7): 1274-1283.
	CAO W D, ZHOU G P, GAO S J. Effects and mechanisms of green manure on endogenous improving soil health. Journal of Plant Nutrition and Fertilizers, 2024, 30(7): 1274-1283. (in Chinese)
[10]	GAO S J, ZHOU G P, CHANG D N, LIANG H, NIE J, LIAO Y L, LU Y H, XU C X, LIU J, WU J, et al. Southern China can produce more high-quality rice with less N by green manuring. Resources, Conservation and Recycling, 2023, 196: 107025.
[11]	LUGATO E, BAMPA F, PANAGOS P, MONTANARELLA L, JONES A. Potential carbon sequestration of European arable soils estimated by modelling a comprehensive set of management practices. Global Change Biology, 2014, 20(11): 3557-3567. doi: 10.1111/gcb.12551 pmid: 24789378
[12]	LIANG H, LI S, ZHOU G P, FU L B, HU F, GAO S J, CAO W D. Targeted regulation of the microbiome by green manuring to promote tobacco growth. Biology and Fertility of Soils, 2024, 60(1): 69-85.
[13]	ZHOU G P, MA Z B, HAN S, CHANG D N, SUN J X, LIU H, ZHOU G D, MA Q X, LIU J, WU J, et al. Green manuring combined with optimal water management achieves a triple-win for paddy soil quality, rice productivity, and environmental benefits. Agriculture, Ecosystems & Environment, 2025, 383: 109507.
[14]	LI Y, ZHANG W, LI J, ZHOU F, LIANG X N, ZHU X F, HE H B, ZHANG X D. Complementation between microbial necromass and plant debris governs the long-term build-up of the soil organic carbon pool in conservation agriculture. Soil Biology and Biochemistry, 2023, 178: 108963.
[15]	CHANG F D, ZHANG H Y, ZHAO P Y, ZHAO N, SONG J S, YU R, WANG J, WANG X Q, HAN D X, LIU X D, ZHOU J, LI Y Y. Green manure roots return drives saline-alkali soil organic carbon accumulation via microbial necromass formation. Soil and Tillage Research, 2025, 251: 106550.
[16]	ANGST G, MUELLER K E, NIEROP K G J, SIMPSON M J. Plant- or microbial-derived: A review on the molecular composition of stabilized soil organic matter. Soil Biology and Biochemistry, 2021, 156: 108189.
[17]	LEHMANN J, KLEBER M. The contentious nature of soil organic matter. Nature, 2015, 528(7580): 60-68.
[18]	MEHRAN M, HUANG L, GENG M J, GAN Y F, CHENG J Y, ZHU Q, ALI AHMAD I, HAIDER S, MUSTAFA A. Co-utilization of green manure with straw return enhances the stability of soil organic carbon by regulating iron-mediated stabilization of aggregate-associated organic carbon in paddy soil. Soil and Tillage Research, 2025, 252: 106624.
[19]	LEE J, HOPMANS J W, ROLSTON D E, BAER S G, SIX J. Determining soil carbon stock changes: Simple bulk density corrections fail. Agriculture, Ecosystems & Environment, 2009, 134(3/4): 251-256.
[20]	鲍士旦. 土壤农化分析. 3版. 北京: 中国农业出版社, 2000.
	BAO S D. Soil and Agricultural Chemistry Analysis. 3rd ed. Beijing: China Agriculture Press, 2000. (in Chinese)
[21]	RENGASAMY P, MARCHUK A. Cation ratio of soil structural stability (CROSS). Soil Research, 2011, 49(3): 280.
[22]	MÁRQUEZ C O, GARCIA V J, CAMBARDELLA C A, SCHULTZ R C, ISENHART T M. Aggregate-size stability distribution and soil stability. Soil Science Society of America Journal, 2004, 68(3): 725-735.
[23]	ISLAM K R, STINE M A, GRUVER J B, SAMSON-LIEBIG S E, WEIL R R. Estimating active carbon for soil quality assessment: A simplified method for laboratory and field use. American Journal of Alternative Agriculture, 2003, 18(1): 3-17.
[24]	JIANG P K, XU Q F, XU Z H, CAO Z H. Seasonal changes in soil labile organic carbon pools within a Phyllostachys praecox stand under high rate fertilization and winter mulch in subtropical China. Forest Ecology and Management, 2006, 236(1): 30-36.
[25]	WRIGHT S F, UPADHYAYA A. Extraction of an abundant and unusual protein from soil and comparison with hyphal protein of arbuscular mycorrhizal fungi. Soil Science, 1996, 161(9): 575-586.
[26]	OTTO A, SIMPSON M J. Degradation and preservation of vascular plant-derived biomarkers in grassland and forest soils from western Canada. Biogeochemistry, 2005, 74(3): 377-409.
[27]	CHEN X B, HU Y J, XIA Y H, ZHENG S M, MA C, RUI Y C, HE H B, HUANG D Y, ZHANG Z H, GE T D, et al. Contrasting pathways of carbon sequestration in paddy and upland soils. Global Change Biology, 2021, 27(11): 2478-2490. doi: 10.1111/gcb.15595 pmid: 33713528
[28]	ZHANG X D, AMELUNG W. Gas chromatographic determination of muramic acid, glucosamine, mannosamine, and galactosamine in soils. Soil Biology and Biochemistry, 1996, 28(9): 1201-1206.
[29]	MENG D, ZHANG K, LIU Z P, ZANG Y X, LIU S S, YANG J Y, BAI E. Refining amino sugar-based conversion factors for quantification of microbial necromass carbon in soils. Global Change Biology, 2025, 31(8): e70443.
[30]	LI S, CHENG D D, YANG Y C, YAO Y Y, GAO B, ZHANG Y H, SHI M M, YU S Q, HAO Q, SONG H F, ZHANG B, WU H Y. Remediation of coastal saline soils using organic amendments: Review. Chemistry and Ecology, 2025, 41(9): 1273-1301.
[31]	PARK S, LEE J G. Green manure improves humification and aggregate stability in paddy soils. Soil Biology and Biochemistry, 2025, 206: 109796.
[32]	闫桂菀, 董文斌, 李忠义, 韦彩会, 唐红琴, 邓羽松, 张宇亭, 何铁光. 绿肥覆盖对果园土壤团聚体及有机碳组分的影响. 应用生态学报, 2024, 35(12): 3427-3434. doi: 10.13287/j.1001-9332.202412.012
	YAN G W, DONG W B, LI Z Y, WEI C H, TANG H Q, DENG Y S, ZHANG Y T, HE T G. Effects of green manure mulching on soil aggregates and organic carbon fractions in orchards. Chinese Journal of Applied Ecology, 2024, 35(12): 3427-3434. (in Chinese) doi: 10.13287/j.1001-9332.202412.012
[33]	LI Y J, JIA X X, ZHAO W L, GAO R C, LUO W, WANG T S. Changes in soil aggregates and aggregate-associated carbon following green manure-maize rotations in coastal saline soil. Agronomy, 2025, 15(6): 1283.
[34]	ZHANG D B, YAO P W, ZHAO N, CAO W D, ZHANG S Q, LI Y Y, HUANG D L, ZHAI B N, WANG Z H, GAO Y J. Building up the soil carbon pool via the cultivation of green manure crops in the Loess Plateau of China. Geoderma, 2019, 337: 425-433.
[35]	杨滨娟, 黄国勤, 兰延, 陈洪俊, 王淑彬. 施氮和冬种绿肥对土壤活性有机碳及碳库管理指数的影响. 应用生态学报, 2014, 25(10): 2907-2913.
	YANG B J, HUANG G Q, LAN Y, CHEN H J, WANG S B. Effects of nitrogen application and winter green manure on soil active organic carbon and the soil carbon pool management index. Chinese Journal of Applied Ecology, 2014, 25(10): 2907-2913. (in Chinese)
[36]	CHANG F D, ZHANG H Y, ZHAO N, ZHAO P Y, SONG J S, YU R, KAN Z R, WANG X Q, WANG J, LIU H J, et al. Green manure removal with reduced nitrogen improves saline-alkali soil organic carbon storage in a wheat-green manure cropping system. Science of the Total Environment, 2024, 926: 171827.
[37]	王国璀, 胡发龙, 李含婷, 殷文, 樊志龙, 范虹, 柴强, 曹卫东. 绿肥提高农田土壤有机碳固存机制的研究进展. 植物营养与肥料学报, 2024, 30(6): 1185-1198.
	WANG G C, HU F L, LI H T, YIN W, FAN Z L, FAN H, CHAI Q, CAO W D. Research advances on the effect and mechanism of green manure on improving soil carbon sequestration in cropland. Journal of Plant Nutrition and Fertilizers, 2024, 30(6): 1185-1198. (in Chinese)
[38]	赵秋, 田秀平, 周丽平, 代雪宾, 宁晓光, 张新建. 不同冬绿肥翻压对土壤有机氮组分和氮素矿化的影响. 水土保持通报, 2023, 43(1): 78-83.
	ZHAO Q, TIAN X P, ZHOU L P, DAI X B, NING X G, ZHANG X J. Effect of different winter green manures on composition and mineralization of soil organic nitrogen. Bulletin of Soil and Water Conservation, 2023, 43(1): 78-83. (in Chinese)
[39]	刘冲, 洪立洲, 郁凯, 董静, 陈环宇, 邢锦城. 江苏沿海滩涂绿肥种植模式对土壤水稳性团聚体及有机碳含量的影响. 江苏农业科学, 2023, 51(12): 180-186.
	LIU C, HONG L Z, YU K, DONG J, CHEN H Y, XING J C. Effects of green fertilizer planting patterns on soil water stability aggregates and organic carbon content in Jiangsu coastal beaches. Jiangsu Agricultural Sciences, 2023, 51(12): 180-186. (in Chinese)
[40]	吕奕彤, 于爱忠, 吕汉强, 王玉珑, 苏向向, 柴强. 绿洲灌区玉米农田土壤团聚体组成及其稳定性对绿肥还田方式的响应. 中国生态农业学报(中英文), 2021, 29(7): 1194-1204.
	LÜ Y T, YU A Z, LÜ H Q, WANG Y L, SU X X, CHAI Q. Composition and stability of soil aggregates in maize farmlands under different green manure utilization patterns in an oasis irrigation area. Chinese Journal of Eco-Agriculture, 2021, 29(7): 1194-1204. (in Chinese)
[41]	SHARMA P, LAOR Y, RAVIV M, MEDINA S, SAADI I, KRASNOVSKY A, VAGER M, LEVY G J, BAR-TAL A, BORISOVER M. Green manure as part of organic management cycle: Effects on changes in organic matter characteristics across the soil profile. Geoderma, 2017, 305: 197-207.
[42]	SIX J, BOSSUYT H, DEGRYZE S, DENEF K. A history of research on the link between (micro)aggregates, soil biota, and soil organic matter dynamics. Soil and Tillage Research, 2004, 79(1): 7-31.
[43]	FEI Y H, SHE D L, YI J, SUN X Q, HAN X, LIU D D, LIU M X, ZHANG H L. Roles of soil amendments in the water and salt transport of coastal saline soils through regulation of microstructure. Land Degradation & Development, 2024, 35(7): 2382-2394.
[44]	WANG Y D, HU N, GE T D, KUZYAKOV Y, WANG Z L, LI Z F, TANG Z, CHEN Y, WU C Y, LOU Y L. Soil aggregation regulates distributions of carbon, microbial community and enzyme activities after 23-year manure amendment. Applied Soil Ecology, 2017, 111: 65-72.
[45]	程琪, 毛霞丽, 孙涛, 王湘洁, 马庆旭, 吴良欢. 长期化肥与不同有机物料配施对土壤微生物生态化学计量特征和群落结构的影响. 植物营养与肥料学报, 2024, 30(2): 209-220.
	CHENG Q, MAO X L, SUN T, WANG X J, MA Q X, WU L H. Effects of long-term combined application of chemical fertilizers with different organic materials on soil microbial ecological stoichiometry and community structure. Journal of Plant Nutrition and Fertilizers, 2024, 30(2): 209-220. (in Chinese)
[46]	RILLIG M C. Arbuscular mycorrhizae, glomalin, and soil aggregation. Canadian Journal of Soil Science, 2004, 84(4): 355-363.
[47]	WALKER B A R, POWELL S M, TEGG R S, DOYLE R B, HUNT I G, WILSON C R. Ten years of green manuring and biofumigation alters soil characteristics and microbiota. Applied Soil Ecology, 2023, 187: 104836.
[48]	OTTO A, SIMPSON M J. Evaluation of CuO oxidation parameters for determining the source and stage of lignin degradation in soil. Biogeochemistry, 2006, 80(2): 121-142.
[49]	KONG A Y Y, SIX J. Tracing root vs. residue carbon into soils from conventional and alternative cropping systems. Soil Science Society of America Journal, 2010, 74(4): 1201-1210.
[50]	LEASEBURG E E, LEI L L, FINK L S. Effects of organic amendments on phenol oxidase, peroxidase, urease, and nitrogen mineralization: A laboratory incubation study. Agrochemicals, 2022, 1(1): 3-16.
[51]	LIANG C, SCHIMEL J P, JASTROW J D. The importance of anabolism in microbial control over soil carbon storage. Nature Microbiology, 2017, 2: 17105. doi: 10.1038/nmicrobiol.2017.105 pmid: 28741607
[52]	XIA S P, SONG Z L, LI Q, GUO L D, YU C X, SINGH B P, FU X L, CHEN C M, WANG Y D, WANG H L. Distribution, sources, and decomposition of soil organic matter along a salinity gradient in estuarine wetlands characterized by C: N ratio, δ¹³C-δ¹⁵N, and lignin biomarker. Global Change Biology, 2021, 27(2): 417-434.
[53]	BLAGODATSKAYA Е, KUZYAKOV Y. Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure: Critical review. Biology and Fertility of Soils, 2008, 45(2): 115-131.
[54]	SOKOL N W, SLESSAREV E, MARSCHMANN G L, NICOLAS A, BLAZEWICZ S J, BRODIE E L, FIRESTONE M K, FOLEY M M, HESTRIN R, HUNGATE B A, et al. Life and death in the soil microbiome: How ecological processes influence biogeochemistry. Nature Reviews Microbiology, 2022, 20(7): 415-430. doi: 10.1038/s41579-022-00695-z pmid: 35228712

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处理 Treatments	容重 Bulk density (g·cm^-3)	含量 Concentrations (g·kg^-1)		碳氮比 SOC/TN	储量 Storage (Mg·hm^-2)
处理 Treatments	容重 Bulk density (g·cm^-3)	土壤有机碳SOC	全氮 TN	碳氮比 SOC/TN	SOC	TN
CK	1.16±0.03a	9.96±0.70b	0.85±0.07b	11.71±0.19a	20.43±1.43b	1.75±0.15b
OV	1.06±0.03a	11.18±0.51ab	1.04±0.06a	10.71±0.09b	22.92±1.06ab	2.14±0.12a
TG	1.05±0.04a	12.13±0.30a	1.16±0.02a	10.45±0.42b	24.88±0.61a	2.38±0.04a
WW	1.07±0.07a	11.42±0.57ab	1.09±0.04a	10.49±0.13b	23.41±1.17ab	2.23±0.09a

处理 Treatments	pH 水土比2.5:1	EC (dS·m^-1)	CEC (cmol·kg^-1)	SAR ((mmol·L^-1)^1/2)	CROSS ((mmol·L^-1)^1/2)
CK	8.40±0.03a	0.139±0.01b	6.55±0.24a	0.70±0.07a	0.75±0.07a
OV	8.21±0.06ab	0.141±0.01b	5.88±0.24a	0.71±0.03a	0.79±0.02a
TG	8.10±0.02ab	0.122±0.01b	6.09±0.15a	0.53±0.04b	0.60±0.03b
WW	7.80±0.06b	0.193±0.02a	6.11±0.07a	0.68±0.06a	0.74±0.06a

Winter Green Manure Enhances Soil Aggregation and Plant- and Microbial-Derived Carbon Sequestration in Coastal Saline-Alkali Soils

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