马铃薯根系分泌物对马铃薯金线虫孵化和趋化的影响及外源酸类物质的验证

doi:10.3864/j.issn.0578-1752.2024.11.009

Abstract

Abstract:

【Objective】The objective of this study is explore the effects of root exudates from different resistant potato varieties and different growth stages on the hatching and chemotaxis of Globodera rostochiensis, so as to provide a theoretical basis for the green prevention and control of G. rostochiensis.【Method】The root exudates of four potato varieties at three growth stages were collected. The effects of root exudates on the hatching and chemotaxis of G. rostochiensis were studied by the soaking method and filter paper method. The components of root exudate were analyzed by GC-MS. Eight standard exogenous acids, caffeic acid, succinic acid, 3-phenylpropionic acid, 3-hydroxypropionic acid, 3-amino-3-methyl-butyric acid, glycolic acid, stearic acid, and L-lactic acid, were selected, and the effects of the main acids of root exudates on the hatching and chemotaxis of G. rostochiensis were verified by the soaking method and filter paper method.【Result】The efficiency of stimulating hatching of G. rostochiensis by root exudates of susceptible varieties Qingshu 9 and Huize 2 was higher than that of resistant varieties Xuanshu 6 and Yunshu 505. At three growth stages (15 d (germination stage), 30 d (seedling stage), 55 d (tuber formation stage)), the effect of susceptible varieties on hatching stimulation was stronger than that of resistant varieties, and the effect of stimulating hatching of each variety root exudate at 30 d (seedling stage) was better than that at 15 d (germination stage) and 55 d (tuber formation stage). The stimulating hatchability of root exudates from Qingshu 9 was 7.20%, 27.30%, and 5.70% at 15, 30 and 55 d, respectively; that of Huize 2 was 10.50%, 38.00%, and 9.40%, Xuanshu 6 was 5.40%, 20.40%, and 16.30%, Yunshu 505 was 13.20%, 21.80%, and 3.10% at 15, 30 and 55 d, respectively. Among the root exudates of four potato varieties, Qingshu 9 was more attractive to G. rostochiensis. At three growth stages, except for Xuanshu 6, the root exudates of the other three varieties at 15 d had a better attraction effect than at 30 and 55 d. The attraction rate of root exudates of Qingshu 9 at 15, 30 and 55 d was 13.73%, 9.68%, and 6.13%, respectively, that of Huize 2 was 5.65%, 4.76%, and 4.89%, Yunshu 505 was 4.86%, 4.13%, and 2.91%, Xuanshu 6 was 4.11%, 5.77%, and 6.47% at 15, 30 and 55 d, respectively. According to GC-MS analysis and identification, potato root exudates mainly contain 9 kinds of substances, including 24 acids, 13 amino acids, 5 salts, 3 alcohols, 3 hydrocarbons, 3 carbohydrates, 2 phenols, 1 ketone, and 1 lipid. From the species and proportion, acids, amino acids, phenols and alcohols are the main components of potato root exudates. The experiment of 8 kinds of exogenous acid substances showed that succinic acid and 3-hydroxypropionic acid had a significant effect on the hatching stimulation of G. rostochiensis. Caffeic acid and 3-phenylpropionic acid had a significant effect on the chemotaxis of G. rostochiensis. However, compared with the effects of root exudates from four varieties at 30 d on G. rostochiensis, it was found that the hatching stimulation effects of above acids with significant effects were significantly lower than those of root exudates, and there was no significant difference in chemotaxis.【Conclusion】The root exudates from different potato varieties and different growth stages all have effects on the hatching and chemotaxis of G. rostochiensis. The effects of hatching and chemotaxis of G. rostochiensis by root exudates from susceptible varieties were higher than those of resistant varieties. The effects of susceptible and resistant varieties on G. rostochiensis began at 15 d (germination stage) when the root system appeared, were strongest at 30 d (seedling stage), and weakened at 55 d (tuber formation stage). The potato root exudates are diverse, the main components are acids, amino acids, and alcohols, and the resistance and susceptible varieties are slightly different. Succinic acid and 3-hydroxypropionic acid had a significant effect on hatching, caffeic acid and 3-phenylpropionic acid had a significant effect on chemotaxis. Single acid had significantly less effect on hatching of G. rostochiensis than root exudates.

Key words: potato (Solanum tuberosum), root exudate, Globodera rostochiensis, hatching, chemotaxis, component

YIN YanDie, YANG YanMei, FU QiChun, WANG Qin, LI YongQing, DUAN JinFeng, LIU YuZhu, WANG QiaoMei, HU XianQi. The Effects of Potato Root Exudates on the Hatching and Chemotaxis of Globodera rostochiensis and Verification of Exogenous Acid Substances[J].Scientia Agricultura Sinica, 2024, 57(11): 2161-2175.

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处理 Treatment	2龄幼虫占总接种量比率Percent of J2 to total number (%)
处理 Treatment	0.5-1 cm	1-2 cm	2-3 cm
宣薯6号Xuanshu 6 15 d	4.11±1.54a	15.22±3.62b	80.67±3.43a
宣薯6号Xuanshu 6 30 d	5.77±1.51a	26.19±10.32b	68.05±11.31b
宣薯6号Xuanshu 6 55 d	6.47±2.61a	24.14±5.67b	69.39±7.15b
ddH₂O	6.97±3.38a	22.22±7.99b	72.21±4.51ab
CaCl₂	5.80±3.13a	39.60±5.25a	54.61±5.71c
云薯505 Yunshu 505 15 d	4.86±3.61a	22.50±7.98b	72.60±10.32a
云薯505 Yunshu 505 30 d	4.13±3.56a	20.94±8.89b	74.94±10.07a
云薯505 Yunshu 505 55 d	2.91±1.10a	18.12±11.11b	78.97±11.06a
ddH₂O	5.58±2.29a	23.92±7.75b	70.25±10.04a
CaCl₂	5.80±3.13a	39.60±5.25a	54.61±5.71b
青薯9号Qingshu 9 15 d	13.73±6.20a	32.59±2.48a	53.68±6.86a
青薯9号Qingshu 9 30 d	9.68±5.11ab	28.10±8.81a	62.22±12.63a
青薯9号Qingshu 9 55 d	6.13±0.91b	35.16±14.00a	58.71±13.63a
ddH₂O	9.51±2.04ab	33.82±5.35a	56.67±5.54a
CaCl₂	5.80±3.13b	39.60±5.25a	54.61±5.71a
会-2 Huize 2 15 d	5.65±0.99a	20.84±5.41bc	74.19±4.45ab
会-2 Huize 2 30 d	4.76±2.72a	29.98±10.77b	65.26±12.08b
会-2 Huize 2 55 d	4.89±1.19a	18.53±3.73c	76.58±4.77a
ddH₂O	4.72±1.96a	28.38±3.70b	68.30±5.19ab
CaCl₂	5.80±3.13a	39.60±5.25a	54.61±5.71c

物质/品种 Substance/Variety	感病品种Susceptible variety						抗病品种Resistant variety
物质/品种 Substance/Variety	青薯9号Qingshu 9 15 d	青薯9号Qingshu 9 30 d	青薯9号Qingshu 9 55 d	会-2 Huize 2 15 d	会-2 Huize 2 30 d	会-2 Huize 2 55 d	宣薯6号Xuanshu 6 15 d	宣薯6号Xuanshu 6 30 d	宣薯6号Xuanshu 6 55 d	云薯505 Yunshu 505 15 d	云薯505 Yunshu 505 30 d	云薯505 Yunshu 505 55 d
咖啡酸Caffeic acid	-	-	-	-	-	-	-	-	-	-	0.19	-
苹果酸Malic acid	-	-	-	-	5.17	-	-	-	-	-	0.29	-
Polyglyceric acid	-	-	-	-	0.49	-	-	-	-	-	0.19	-
(2S,3R,4S,5R)-2,3,4,5- Tetrahydroxyhexanedioic acid	-	-	-	-	-	-	-	-	-	-	0.73	-
木糖酸Xylonic acid	-	0.27	-	-	-	-	-	-	-	-	0.24	-
Pentanoic acid	-	1.65	-	-	-	-	-	-	-	-	3.88	-
2,3,4-Trihydroxybutyric acid	-	0.21	-	-	0.66	-	-	-	-	-	0.33	-
丁二酸Succinic acid	-	7.12	15.14	-	10.90	-	-	3.21	-	1.77	5.00	6.01
(S)-3-羟基丁酸 (S)-3-Hydroxybutyric acid	-	0.34	-	-	-	-	-	-	-	-	0.21	0.27
3-氨基-3-甲基丁酸 3-Amino-3-methyl-butyric acid	1.19	1.84	-	-	-	-	-	-	-	-	2.03	-
氢化肉桂酸 3-Phenylpropionic acid	-	0.25	-	-	-	-	-	-	-	-	-	0.71
4-Azanylbutanoic acid	1.01	1.79	-	-	-	-	-	0.70	-	-	1.65	-
3-Deoxypentonic acid	-	-	-	-	-	-	-	-	-	-	1.03	-
硬脂酸Stearic acid	0.76	0.69	3.60	-	2.31	3.72	1.72	1.27	3.91	1.24	0.32	1.56
3-羟基丙酸3-Hydroxypropionic acid	-	-	-	-	-	-	-	0.38	-	-	-	0.36
乙醇酸Glycolic acid	-	-	-	-	-	-	-	-	-	-	0.14	-
L-乳酸L-lactic acid	-	5.21	13.25	-	12.80	-	-	0.29	-	-	4.12	4.11
莽草酸Shikimic acid	-	0.25	-	-	-	-	-	-	-	-	-	-
富马酸Fumaric acid	-	-	-	-	14.05	-	-	-	-	-	-	-
2-氨基戊二酸 DL-glutamic acid	-	-	-	-	-	-	-	2.36	-	-	-	-
2-氨基丁酸DL-2-aminobutyric acid	-	-	-	-	-	-	-	-	-	-	1.65	-
(Propionylamino)acetic acid	-	-	-	-	-	-	-	-	-	-	2.03	-
壬二酸Azelaic acid	-	-	-	-	-	-	-	-	-	-	0.39	-
磷酸Sodium pyrophosphate	-	-	-	-	0.63	-	-	2.66	-	1.59	-	-

	孵化的2龄幼虫数量 Number of hatched J2	吸引率 Attraction rate (%)
会-2 Huize 2	802a	5ab
青薯9号Qingshu 9	766a	10a
宣薯6号Xuanshu 6	470b	6ab
云薯505 Yunshu 505	555b	4ab
ddH₂O	6c	8a
咖啡酸Caffeic acid	8c	2b
丁二酸Succinic acid	127c	5ab
氢化肉桂酸3-Phenylpropionic acid	4c	10a
3-羟基丙酸3-Hydroxypropionic acid	164c	6ab
1% DMSO	8c	6ab

The Effects of Potato Root Exudates on the Hatching and Chemotaxis of Globodera rostochiensis and Verification of Exogenous Acid Substances

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