Scientia Agricultura Sinica ›› 2018, Vol. 51 ›› Issue (11): 2155-2163.doi: 10.3864/j.issn.0578-1752.2018.11.013

• HORTICULTURE • Previous Articles     Next Articles

QTL Fine Mapping and Candidate Gene Prediction for Growth     Traits in G.41×Malus sieversii

DONG Jun, WANG Jing, LIANG Wei, MA BaiQuan, DONG LiJuan, MA FengWang, FU XuanChang, LI CuiYing   

  1. College of Horticulture, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi
  • Received:2017-09-21 Online:2018-06-01 Published:2018-06-01

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Abstract: 【Objective】Dwarf rootstock is an important germplasm to be utilized in the production of high and stable yields of apples by inducing dwarf scions. Mapping the genes that regulate the growth of apple trees has important theoretical and practical significance for improving apple's yield and quality. 【Method】 In this study, 188 F1 progenies derived from the cross G.41×Malus sieversii were used to perform QTL mapping, and ‘Fuji Champion’ were grafted on every F1 plant. Subsequently, based on SSR markers, a genetic map of apple was constructed using Join Map 4.0 software. Using Map QTL 5.0 mapping software, the growth traits, including scion height and cross-sectional area of the scion were used as phenotypic data in QTL preliminary analysis. Combined with information from the apple genome sequence and newly developed SSR markers using Primer 5.0 software, the QTL has been defined and the candidate genes were predicted. 【Result】 In this study, a total of 108 out of 361 SSRs showed a variable degree of polymorphism, and the polymorphism rate was 29.9%. A genetic map was constructed using 95 of 108 polymorphism SSRs and four QTLs for the growth trait were detected in linkage group No.5. Two markers (L05024 and Hi09b04) were closely linked with the height and the cross-sectional area of scion. Combined with the genome sequence, 24 new SSR markers were developed, which contained 10 polymorphism markers. Subsequently, the linkage group No.5 was analyzed again using these 10 markers. The reconstructed high density linkage group, consisted of 21 SSR markers, spanning 86.0 cM with an average of 7.52 cM per marker. The QTLs responsible for the height and the cross-sectional area of the scion were defined and two relevant SSR markers (L05024 and Hi09b04) were identified accounting for 19.2% and 51.7% of the observed phenotypic variation. According to the ‘Gold Delicious’ genome sequence, this locus has been defined in a region of 543 kb that contains 16 candidate genes and a gene (MDP0000323212) was considered as the candidate gene for plant growth in this QTL interval. 【Conclusion】 In this study, genes responsible for dwarfing were located in a 4.048-4.591 Mb interval on apple chromosome No.5 and two high linked SSR markers (L05024 and Hi09b04) were identified. A gene (MDP0000323212) was identified as a potential candidate gene for growth traits.

Key words: apple rootstock, SSR, genetic map, QTL, candidate gene

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