甜菜WRKY转录因子全基因组鉴定及其在非生物胁迫下的表达分析

doi:10.3864/j.issn.0578-1752.2017.17.002

Abstract

Abstract: 【Objective】 WRKY transcription factor is a kind of transcription factor which plays an important role in plant response to biotic, abiotic stress, plant growth and development. Based on the sugar beet genome information, the WRKY family genes (BvWRKYs) were identified, tissue-specific expression profiles and expression pattern under salt and heat stresses were analyzed. The aim of the study is to provide reference for the WRKY gene function research, and lay a foundation for ornamental beet (B. vulgaris) and other Dianthus ornamental plant genetic engineering research. 【Method】 A total of 75 Arabidopsis WRKY proteins were used as references, according to WRKY conserved protein sequence (PF03106), the WRKY genes of B. vulgaris were identified by hmm and BLAST homology searches, and the chromosome location, phylogeny, gene structure, conserved domain, conserved element were also analyzed by MapInspect, GSDS2.0, MEGA5.0, DNAMAN5.0, WebLogo 3 and MEME bioinformatics tools. The specificity of WRKY genes expression and expression pattern under salt stress and heat stress of B. vulgaris were analyzed by RNA-seq and qRT-PCR analysis. 【Result】 B. vulgaris WRKY gene family contained 40 members, 39 were unevenly distributed on 9 chromosomes, 1 on the random fragment. According to the WRKY conserved domain features and the evolution analysis with Arabidopsis thaliana, 40 members were divided into three classes: Ⅰ, Ⅱ, and Ⅲ. ClassⅠhad 9 members, class Ⅱ had 26 members and class Ⅲ had 5 members. According to the evolutionary relationship, class Ⅱ further divided into five subclass: Ⅱa (1), Ⅱb (4), Ⅱc (9), Ⅱd (5) and Ⅱe (7). Genetic structure analysis showed that exon and intron number of B. vulgaris WRKY genes had high variability (2-7 exons), even within the same subgroup. Conserved element analysis showed that within the same class or subclass had the same conserved elements. WRKY conserved domain analysis revealed two mutations in the WRKY domain: WRKYGKK and WRKYGEK. Each WRKY was expressed in at least two tissues, 30 WRKYs were expressed in leaf, 40 WRKYs were expressed in inflorescence, 36 WRKYs were expressed in young leaf, 38 WRKYs were expressed in root, 39 WRKYs were expressed in seedling, and 36 WRKYs were expressed in seed. The expression levels of WRKY genes were also different. All WRKY genes were divided into two types: low expression genes and high expression genes. Such as: BvWRKY23, BvWRKY3, BvWRKY11, BvWRKY7, BvWRKY6, BvWRKY26, BvWRKY4, BvWRKY40, BvWRKY24, BvWRKY2 and BvWRKY28 were highly expressed in all tissues; BvWRKY38, BvWRKY13, BvWRKY36, BvWRKY35, BvWRKY5 and BvWRKY34 were low expressed in all tissues. BvWRKY21, BvWRKY20, BvWRKY22, BvWRKY32, BvWRKY33 and BvWRKY34 genes were up-regulated under heat stress, and BvWRKY1, BvWRKY6, BvWRKY19, BvWRKY31 and BvWRKY33 were up-regulated under salt stress. In addition, BvWRKY33 had a significant response to both heat and salt stress. 【Conclusion】 B. vulgaris WRKY proteins are highly conserved, the length of the gene sequence and the number of introns varied widely, all WRKY genes showed a variety of expression patterns in different tissues, some WRKY genes responded to heat or salt stress, which play an important role in stress physiological regulation.

Key words: Beta vulgaris, WRKY transcription factor, bioinformatics, stress, expression analysis

KONG WeiLong, YU Kun, DAN NaiZhen, YANG ShaoZong, BAO ManZhu, HUANG XiangRong, FU XiaoPeng. Genome-Wide Identification and Expression Analysis of WRKY Transcription Factor Under Abiotic Stress in Beta vulgaris[J].Scientia Agricultura Sinica, 2017, 50(17): 3259-3273.

References

[1] Dohm J C, Minoche A E, Holtgräwe D, Capellagutiérrez S, Zakrzewski F, Tafer H, Rupp O, Sörensen T R, Stracke R, Reinhardt R. The genome of the recently domesticated crop plant sugar beet (Beta vulgaris). Nature, 2014, 505(7484): 546-549.

[2] Eulgem T, Rushton P J, Robatzek S, Somssich I E. The WRKY superfamily of plant transcription factors. Trends in Plant Science, 2000, 5(5): 199-206.

[3] Guo C, Guo R, Xu X, Gao M, Li X, Song J, Zheng Y, Wang X. Evolution and expression analysis of the grape (Vitis vinifera L.) WRKY gene family. Journal of Experimental Botany, 2014, 65(6): 1513-1528.

[4] Cormack R S, Eulgem T, Rushton P J, Köchner P, Hahlbrock K, Somssich I E. Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor- induced WRKY transcription factors in parsley. Biochimica et Biophysica Acta, 2002, 1576(1/2): 92-100.

[5] Somssich I E. WRKY transcription factors: from DNA binding towards biological function. Current Opinion in Plant Biology, 2004, 7(5): 491-498.

[6] Zhang Y, Wang L. The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evolutionary Biology, 2005, 5(1): 1-12.

[7] Ishiguro S, Nakamura K. Characterization of a cDNA encoding a novel DNA-binding protein, SPF1, that recognizes SP8 sequences in the 5' upstream regions of genes coding for sporamin and beta-amylase from sweet potato. Molecular Genetics and Genomics, 1994, 244(6): 563-571.

[8] Dellagi A, Birch P J, Heilbronn J, Avrova A O, Montesano M, Palva E T, Lyon G D. A potato gene, erg-1, is rapidly induced by Erwinia carotovora ssp. atroseptica, Phytophthora infestans, ethylene and salicylic acid. Journal of Plant Physiology, 2000, 157(2): 201-205.

[9] Yoda H, Ogawa M, Yamaguchi Y, Koizumi N, Kusano T, Sano H. Identification of early-responsive genes associated with the hypersensitive response to tobacco Mosaic virus and characterization of a WRKY-type transcription factor in tobacco plants. Molecular Genetics and Genomics, 2002, 267(2): 154-161.

[10] Sun C, Palmqvist S, Olsson H, Borén M, Ahlandsberg S, Jansson C. A novel WRKY transcription factor, SUSIBA2, participates in sugar signaling in barley by binding to the sugar- responsive elements of the iso1 promoter. The Plant Cell, 2003, 15(9): 2076-2092.

[11] 刘自成, 苗丽丽, 王景一, 杨德龙, 毛新国, 景蕊莲. 普通小麦转录因子基因TaWRKY35的克隆及功能分析. 中国农业科学, 2016, 49(12): 2245-2254.
Liu Z C, Miao L L, Wang J Y, Yang D L, Mao X G, Jing R L. Cloning and characterization of transcription factor TaWRKY35 in wheat (Triticum aestivum). Scientia Agricultura Sinica, 2016, 49(12): 2245-2254. (in Chinese)

[12] Chen H, Lai Z, Shi J, Xiao Y, Chen Z, Xu X. Roles of Arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress. BMC Plant Biology, 2010, 10(1): 443-462.

[13] Chen W, Provart N J, Glazebrook J, Katagiri F, Chang H S, Eulgem T, Mauch F, Luan S, Zou G, Whitham S A, BUDWORTH P R, YAO T, XIE Z, CHEN X, LAM S, KREPS J A, HARPER J F, SI-AMMOUR A, MAUCH-MANI B, HEINLEIN M, KOBAYASHI K, HOHN T, DANGL J L, WANG X, ZHU T. Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses. The Plant Cell, 2002, 14(3): 559-574.

[14] Hwang S H, Yie S W, Hwang D J. Heterologous expression of OsWRKY6 gene in Arabidopsis activates the expression of defense related genes and enhances resistance to pathogens. Plant Science, 2011, 181(3): 316-323.

[15] Xu X, Chen C, Fan B, Chen Z. Physical and functional interactions between pathogen-induced Arabidopsis WRKY18, WRKY40, and WRKY60 transcription factors. The Plant Cell, 2006, 18(5): 1310-1326.

[16] ZHANG Z Z, QAMAR S A, Chen Z X, Mengiste T. Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens. Plant Journal for Cell & Molecular Biology, 2006, 48(4): 592-605.

[17] Zheng J, Zou X, Mao Z, Xie B. A novel pepper (Capsicum annuum L.) WRKY gene, CaWRKY30, is involved in pathogen stress responses. Journal of Plant Biology, 2011, 54(5): 329-337.

[18] Ramamoorthy R, Jiang S Y, Kumar N, Venkatesh P N, Ramachandran S. A comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments. Plant and Cell Physiology, 2008, 49(6): 865-879.

[19] Christian R, Shen Q x. The WRKY gene family in rice (Oryza sativa). Journal of Integrative Plant Biology, 2007, 49(6): 827-842.

[20] Zhang J, Peng Y l, GUO Z J. Constitutive expression of pathogen-inducible OsWRKY31 enhances disease resistance and affects root growth and auxin response in transgenic rice plants. Cell Research, 2008, 18(4): 508-521.

[21] Oh S K, Baek K H, Park J M, Kamoun S, Choi D. Capsicum annuum WRKY protein CaWRKY1 is a negative regulator of pathogen defense. New Phytologist, 2008, 177(4): 977-989.

[22] Levée V, Major I, Levasseur C, Tremblay L, Mackay J, Séguin A. Expression profiling and functional analysis of Populus WRKY23 reveals a regulatory role in defense. New Phytologist, 2009, 184(1): 48-70.

[23] Yang B, Jiang Y, Rahman M H, Deyholos M K, Kav N N. Identification and expression analysis of WRKY transcription factor genes in canola (Brassica napus L.) in response to fungal pathogens and hormone treatments. BMC Plant Biology, 2009, 9(1): 261-279.

[24] Ling J, Jiang W, Zhang Y, Yu H, Mao Z, Gu X, Huang S, Xie B. Genome-wide analysis of WRKY gene family in Cucumis sativus. BMC Genomics, 2011, 12(1): 1-20.

[25] Yao D, Zhang X, Zhao X, Liu C, Wang C, Zhang Z, Zhang C, Wei Q, Wang Q, Yan H. Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (Gossypium hirsutum L.). Genomics, 2011, 98(1): 47-55.

[26] Li M Y, Xu Z S, Tian C, Huang Y, Wang F, Xiong A S. Genomic identification of WRKY transcription factors in carrot (Daucus carota) and analysis of evolution and homologous groups for plants. Scientific Reports, 2016, 6(1): 1-17.

[27] Pan L J, Jiang L. Identification and expression of the WRKY transcription factors of Carica papaya in response to abiotic and biotic stresses. Molecular Biology Reports, 2014, 41(3): 1215-1225.

[28] Bi C W, Xu Y Q, Ye Q L, Yin T M, Ye N. Genome-wide identification and characterization of WRKY gene family in Salix suchowensis. PeerJ, 2016, 4: e2437.

[29] 谷彦冰, 冀志蕊, 迟福梅, 乔壮, 徐成楠, 张俊祥, 董庆龙, 周宗山. 苹果WRKY基因家族生物信息学及表达分析. 中国农业科学, 2015, 48(16): 3221-3238.
Gu Y B, Ji Z R, Chi F M, Qiao Z, Xu C N, Zhang J X, Dong Q L, Zhou Z S. Bioinformatics and expression analysis of the WRKY gene family in apple. Scientia Agricultura Sinica, 2015, 48(16): 3221-3238. (in Chinese)

[30] 祖倩丽, 尹丽娟, 徐兆师, 陈明, 周永斌, 李连城, 马有志, 闵东红, 张小红. 谷子WRKY36转录因子的分子特性及功能鉴定. 中国农业科学, 2015, 48(5): 851-860.
Zu Q L, Yin L J, Xu Z S, Chen M, Zhou Y B, Li L C, Ma Y Z, Min D H, Zhang X H. Molecular characteristics and functional identification of foxtail millet transcription factor WRKY36. Scientia Agricultura Sinica, 2015, 48(5): 851-860. (in Chinese)

[31] Rushton P J, Somssich I E, Ringler P, Shen Q J. WRKY transcription factors. Trends in Plant Science, 2014, 15(5): 247-258.

[32] Ren X, Chen Z, Liu Y, Zhang H, Zhang M, Liu Q, Hong X, Zhu J K, Gong Z. ABO3, a WRKY transcription factor, mediates plant responses to abscisic acid and drought tolerance in Arabidopsis. Plant Journal for Cell & Molecular Biology, 2010, 63(3): 417-429.

[33] Niu C F, Wei W, Zhou Q Y, Tian A G, Hao Y J, Zhang W K, Biao M A, Lin Q, Zhang Z B, Zhang J S. Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stress tolerance in transgenic Arabidopsis plants. Plant Cell & Environment, 2012, 35(6): 1156-1170.

[34] Zhen X, Zhang Z L, Zou X, Yang G, Komatsu S, Shen Q J. Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells. Plant Journal for Cell & Molecular Biology, 2006, 46(2): 231-242.

[35] Minoche A E, Dohm J C, Schneider J, Holtgräwe D, Viehöver P, Montfort M, Sörensen T R, Weisshaar B, Himmelbauer H. Exploiting single-molecule transcript sequencing for eukaryotic gene prediction. Genome Biology, 2015, 16(1): 1-13.

[36] Stracke R, Holtgräwe D, Schneider J, Pucker B, Sörensen T R, Weisshaar B. Genome-wide identification and characterization of R2R3-MYB genes in sugar beet (Beta vulgaris). BMC Plant Biology, 2014, 14(1): 1-17.

[37] 梁娜, 伍国强, 冯瑞军, 刘左军, 李志忠. 甜菜Actin基因片段的克隆及序列分析. 中国糖料, 2014(4): 13-15.
Liang N, Wu G J, Feng R J, Liu Z J, Li Z Z. Cloning and sequence analysis of Actin gene fragments from sugarbeet. Sugar Crops of China, 2014(4): 13-15. (in Chinese)

[38] Huang S, Gao Y, Liu J, Peng X, Niu X, Fei Z, Cao S, Liu Y. Genome-wide analysis of WRKY transcription factors in Solanum lycopersicum. Molecular Genetics and Genomics, 2012, 287(6): 495-513.

[39] Hu L, Liu S. Genome-wide analysis of the MADS-box gene family in cucumber. Genome, 2012, 55(3): 245-256.

[40] Li H L, Zhang L B, Guo D, Li C Z, Peng S Q. Identification and expression profiles of the WRKY transcription factor family in Ricinus communis. Gene, 2012, 503(2): 248-253.

[41] He H, Dong Q, Shao Y, Jiang H, Zhu S, Cheng B, Xiang Y. Genome-wide survey and characterization of the WRKY gene family in Populus trichocarpa. Plant Cell Reports, 2012, 31(7): 1199-1217.

[42] Yu Y, Wang N, Hu R, Xiang F. Genome-wide identification of soybean WRKY transcription factors in response to salt stress. Springerplus, 2016, 5(1): 1-15.

[43] Dou L, Zhang X, Pang C, Song M, Wei H, Fan S, Yu S. Genome-wide analysis of the WRKY gene family in cotton. Molecular Genetics and Genomics, 2014, 289(6): 1103-1121.

[44] Tang J, Wang F, Hou X L, Wang Z, Huang Z N. Genome-wide fractionation and Identification of WRKY transcription factors in Chinese cabbage (Brassica rapa ssp. pekinensis) reveals collinearity and their expression patterns under abiotic and biotic stresses. Plant Molecular Biology Reporter, 2013, 32(4): 1-15.

[45] Song H, Wang P, Lin J Y, Zhao C, Bi Y, Wang X. Genome-wide identification and characterization of WRKY gene family in peanut. Frontiers in Plant Science, 2016, 7(1): 1-16.

[46] Huang S, Gao Y, Liu J, Peng X, Niu X, Fei Z, Cao S, Liu Y. Genome-wide analysis of WRKY transcription factors in Solanum lycopersicum. Molecular Genetics & Genomics, 2012, 287(6): 495-513.

[47] Chen M, Tan Q, Sun M, Li D, Fu X, Chen X, Xiao W, Li L, Gao D. Genome-wide identification of WRKY family genes in peach and analysis of WRKY expression during bud dormancy. Molecular Genetics and Genomics, 2016, 291(3): 1319-1332.

[48] Zhou Q Y, Tian A G, Zou H F, Xie Z M, Lei G, Huang J, Wang C M, Wang H W, Zhang J S, Chen S Y. Soybean WRKY-type transcription factor genes, GmWRKY13, GmWRKY21, and GmWRKY54, confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants. Plant Biotechnology Journal, 2008, 6(5): 486-503.

[49] Van verk M C, Pappaioannou D, Neeleman L, Bol J F, Linthorst H J. A novel WRKY transcription factor is required for induction of PR-1a gene expression by salicylic acid and bacterial elicitors. Plant Physiology, 2008, 146(4): 1983-1995.

[50] Li C, Li D, Shao F, Lu S. Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza. BMC Genomics, 2015, 16(1): 1-21.

[51] Dai X. OsWRKY74, a WRKY transcription factor, modulates tolerance to phosphate starvation in rice. Journal of Experimental Botany, 2016, 67(3): 823-826.

[52] Su T, Xu Q, Zhang F C, Chen Y, Li L Q, Wu W H, Chen Y F. WRKY42 modulates phosphate homeostasis through regulating phosphate translocation and acquisition in Arabidopsis. Plant Physiology, 2015, 167(4): 1579-1591.

[53] Wang H, Xu Q, Kong Y H, Chen Y, Duan J Y, Wu W H, Chen Y F. Arabidopsis WRKY45 transcription factor activates PHOSPHATE TRANSPORTER1;1 expression in response to phosphate starvation. Plant Physiology, 2014, 164(4): 2020-2029.

[54] Wu H, Ni Z, Yao Y, Guo G, Sun Q. Cloning and expression profiles of 15 genes encoding WRKY transcription factor in wheat (Triticum aestivem L.). Progress in Natural Science: Materials International, 2008, 18(6): 697-705.

[55] QIU Y P, JING S J, FU J, LI L, YU D Q. Cloning and analysis of expression profile of 13 WRKY genes in rice. Science Bulletin, 2004, 49(20): 2159-2168.

[56] 黄胜雄, 刘永胜. 土豆WRKY转录因子家族的生物信息学分析. 应用与环境生物学报, 2013, 19(2): 205-214.
Huang S X, Liu Y S. Genome-wide analysis of WRKY transcription factors in Solanum tuberosum. Chinese Journal of Applied and Environmental Biology, 2013, 19(2): 205-214. (in Chinese)

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Genome-Wide Identification and Expression Analysis of WRKY Transcription Factor Under Abiotic Stress in Beta vulgaris

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