氮素对灌浆期夏玉米叶片蛋白质表达的调控

doi:10.3864/j.issn.0578-1752.2015.09.06

Abstract

Abstract: 【Objective】The objective of this experiment is to explore the regulating effect of nitrogen on protein expression of leaf of summer maize under the field production condition.【Method】Denghai 618 was selected as an experimental material, different nitrogen treatments were designed to investigate the photosynthetic rate, the activity of nitrate reductase (NR), superoxide dismutase (SOD) and peroxidase (POD), malondialdehyde (MDA) content and soluble protein content in maize ear leaves after tasseling. The total proteins from harvested sample at the stage of 20 days after tasseling were extracted by the trichloroacetate (TCA)-acetone precipitation method and the protein profiles of maize ear leaves were set up by two-dimensional electrophoresis (2-DE). The proteins in maize leaves responding to nitrogen were identified by ImageMaster-2D Elite 7.0 software. The functions of these differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analysis and NCBInr database searching.【Result】After tasseling, the photosynthetic rate, activities of nitrate reductase (NR), superoxide dismutase (SOD) and peroxidase (POD), and soluble protein content were significantly decreased, and malondialdehyde (MDA) content was significantly increased. The photosynthetic rate, NR, SOD and POD, and soluble protein content were significantly increased, and MDA content was significantly decreased in nitrogen supply treatment when compared to nitrogen limited treatment. There were 1 086 and 1 170 protein spots were found after 2-DE analysis in nitrogen limited and nitrogen supply treatment, respectively. After a comparative proteomics analysis and MALDI-TOF/TOF mass spectrometry (MS), 29 differentially expressed proteins were found, including 25 which were successfully identified. They were all up-regulated in nitrogen supply treatment except spots 55 and 1089. They were mainly involved in energy, defense-related pathways, protein synthesis, metabolism, protein transportation and storage, cell growth, secondary metabolism, transcription and unknown.【Conclusion】Nitrogen supply can significantly improve the photosynthesis, carbohydrate catabolism, defense-related pathways, protein synthesis, protein transportation and storage and secondary metabolism in maize leaves at filling stage.

Key words: nitrogen, summer maize, ear leaf, comparative proteomics, filling stage

WANG Xiang-yu, WEI Shan-shan, DONG Shu-ting, LIU Peng, ZHANG Ji-wang, ZHAO Bin. Regulation of Nitrogen on Protein Expression of Summer Maize (Zea mays L.) Leaves at Filling Stage[J].Scientia Agricultura Sinica, 2015, 48(9): 1727-1736.

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