烟蚜茧蜂触角的超微结构与冷藏对触角的影响

doi:10.3864/j.issn.0578-1752.2014.23.009

Abstract

Abstract: 【Objective】 The objectives of this study are to describe the external morphology of the antennae and antennal sensilla of Aphidius gifuensis, examine the effects of cold storage on the antennal sensilla, and to provide the basis for effective use of A. gifuensis . 【Method】 Scanning electron microscopy was used to observe the morphological characteristics of the antennae and sensilla of A. gifuensis adults that emerged under normal conditions, and to compare the sensilla of males and females. Mature larvae of A. gifuensis were stored at 4℃ for 7, 14, 21, and 28 days, and then following cold storage, samples were prepared by ethanol dehydration. Scanning electron microscopy was used to study the effects of cold storage on the external form of the antennae and antennal sensilla. 【Result】 The antennae of males and females were both filiform and had similar morphologies. The average length of the male antenna (19 segments) was slightly longer than that of the female (17 segments). Six types of antennal sensilla were identified, namely, trichodea, chaetica, placodea, Böhm’s bristles, coeleoconica, and basiconica sensilla. Sensilla trichodea type I, which were the most widely distributed and most numerous of the antennal sensilla, were found mainly on the flagella. Sensilla placodea were also distributed on the flagella, occurring from the first flagellum in males and from the second flagellum in females. Sensilla coeleoconica type II were found only on the antennae of adult males. There were differences in the number of different sensilla on male and female antennae. The number of sensilla chaetica type I in females was greater than that in males, whereas the converse was true for the number of sensilla chaetica type II and sensilla placodea. Abnormal placodea and coeleoconica were observed when larvae were stored at 4℃ for 14, 21, and 28 days, with the low temperature influencing the segmentation of the last two antennal subsegments. Fifty-four percent of male antennae showed abnormal segmentation, with antennae comprising 18 segments instead of the normal 19. Similarly, 69% of female antennae showed abnormal segmentation, bearing 16 segments instead of the typical 17. 【Conclusion】The antennae of adult male and female A. gifuensis are filiform. Six types of antennal sensilla were found on the antennae. The types of antennal sensilla in both males and females were similar, but the length of the male antenna was longer than that of the female. Cold storage had a significant effect on antennal segmentation and the form of some sensilla. In order to reduce the damage to A. gifuensis caused by cold storage,preserving A. gifuensis prior to the pupal stageshould be avoided and the storage time should be shortened.

Key words: Aphidius gifuensis, scanning electron microscopy, antennal sensilla, cold storage, deformity

SUN Zhi-juan, CHEN Dan, FAN Xiu-juan, LIU Li, CHENG Yun-ji, ZHANG Chang-hua, REN Guang-wei, LIU Xiang-dong. Antennal Ultrastructure of Aphidius gifuensis and the Effect of Cold Storage on Antennae[J].Scientia Agricultura Sinica, 2014, 47(23): 4637-4647.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2014.23.009

https://www.chinaagrisci.com/EN/Y2014/V47/I23/4637

References

[1] 吴兴富, 李天飞, 魏佳宁, 王毅, 邓建华, 高家合, 赵立恒. 温度对烟蚜茧蜂发育、生殖的影响. 动物学研究, 2000, 21(3): 192-198.

Wu X F, Li T F, Wei J N, Wang Y, Deng J H, Gao J H, Zhao L H. Temperature effect on development and fecundity of Aphidius gifuensisAshmead. Zoological Research, 2000, 21(3): 192-198. (in Chinese)

[2] 任广伟, 秦焕菊, 史万华, 王桂芬. 我国烟蚜茧蜂的研究进展. 中国烟草科学, 2000, 21(1): 27-30.

Ren G W, Qin H J, Shi W H, Wang G F. Review on research of Aphidius gifuensis in China. Chinese Tobacco Science, 2000, 21(1): 27-30. (in Chinese)

[3] 严善春, 张丹丹, 迟德富. 植物挥发性物质对昆虫作用的研究进展. 应用生态学报, 2003, 14(2): 310-313.

Yan S C, Zhang D D, Chi D F. Advances of studies on the effects of plant volatiles on insect behavior. Chinese Journal of Applied Ecology, 2003, 14(2): 310-313. (in Chinese)

[4] 马瑞燕, 杜家纬. 昆虫的触角感器. 昆虫知识, 2000, 37(3): 179-183.

Ma R Y, Du J W. Insect antennal sensilla. Chinese Bulletin of Entomology, 2000, 37(3): 179-183. (in Chinese)

[5] 李竹, 陈力. 触角感器特征应用于昆虫分类的研究进展. 昆虫分类学报, 2010, 32(增刊): 113-118.

Li Z, Chen L. Application of antennal sensilla characters in insect taxonomy. Entomotaxonmia, 2010, 32(Suppl.): 113-118. (in Chinese)

[6] 韩宝瑜. 茶蚜和蚜茧蜂触角感器的扫描电镜观察. 茶业通报, 2000, 22(1): 25-26.

Han B Y. Antennal sensilla of Toxoptera aurantii Boyer and Aphidius sp. observed with scanning electron microscopy. Journal of Tea Business, 2000, 22(1): 25-26. (in Chinese)

[7] Xi Y Q, Yin X M, Li X J, Zhu C D, Zhang Y Z. Scanning electron microscopy studies of antennal sensilla of Lysiphlebus fabarum (Marshall) (Hymenoptera: Braconidae). Acta Entomologica Sinica, 2010, 53(8): 936-942.

[8] 李晶津, 钱海涛, 董辉, 丛斌, 李瑭. 麦蛾茧蜂触角感器的扫描电镜观察. 昆虫知识, 2008, 45(1): 61-64.

Li J J, Qian H T, Dong H, Cong B, Li T. Observing antennal sensilla of Bracon hebetor using scanning electron microscopy. Chinese Bulletin of Entomology, 2008, 45(1): 61-64. (in Chinese)

[9] 陈新芳, 高燕, 章潜才. 侧沟茧蜂触角感觉器的扫描电镜观察. 昆虫天敌, 2004, 26(4): 169-174.

Chen X F, Gao Y, Zhang Q C. Scanning electron microscopy of antennal sensilla of Microplitis sp.. Nature Enemies of Insects, 2004, 26(4): 169-174. (in Chinese)

[10] 唐文颖, 张燕, 郑方强, 墨铁路, 刘同先, 许永玉. 变温对烟蚜茧蜂低温贮藏存活特性的影响. 中国农业科学, 2011, 44(3): 493-499.

Tang W Y, Zhang Y, Zheng F Q, Mo T L, Liu T X, Xu Y Y. Effects of fluctuating thermal regimes on the survival of cold-exposed parasitoid wasps Aphidius gifuensis Ashmead. Scientia Agricultura Sinica, 2011, 44(3): 493-499. (in Chinese)

[11] 吴兴富, 赵立恒, 魏佳宁, 李天飞, 王毅, 邓建华, 高家合. 烟田烟蚜茧蜂的活动规律及其对烟蚜的防治效果. 西南农业大学学报: 自然科学版, 2000, 22(4): 327-330.

Wu X F, Zhao L H, Wei J N, Li T F, Wang Y, Deng J H, Gao J H. Activity rhythm of Aphidius gifuensis Ashmead in tobacco fields and its control of tobacco aphid. Journal of Southwest Agricultural University: Natural Science Edition, 2000, 22(4): 327-330. (in Chinese)

[12] 吴兴富. 烟蚜茧蜂繁殖利用概述. 中国农学通报, 2007, 23(5): 306-308.

Wu X F. Summarize on the using and reproducing of Aphidius gifuensis (Aphidiidae). Chinese Agricultural Science Bulletin, 2007, 23(5): 306-308. (in Chinese)

[13] 陈茂华, 韩召军, 王瑞. 烟蚜茧蜂蛹期耐冷藏性研究. 植物保护, 2005, 31(2): 41-43.

Chen M H, Han Z J, Wang R. A preliminary study on the effects of cold storage on the pupae of Aphidius gifuensis Ashmead. Plant Protection, 2005, 31(2): 41-43. (in Chinese)

[14] 陈文龙, 闫玉芳. 冷藏处理对烟蚜茧蜂羽化率及寿命的影响. 西南大学学报: 自然科学版, 2012, 34(8): 1-5.

Chen W L, Yan Y F. The impact of refrigeration on the emergence rate and life span of Aphidius gifuensis (Ashmead). Journal of Southwest University: Natural Science Edition, 2012, 34(8): 1-5. (in Chinese)

[15] 董兆克, 戈峰. 温度升高对昆虫发生发展的影响. 应用昆虫学报, 2011, 48(5): 1141-1148.

Dong Z K, Ge F. The fitness of insects in response to climate warming. Chinese Journal of Applied Entomology, 2011, 48(5): 1141-1148. (in Chinese)

[16] Bleeker M A K, Smid H M, Van Aelst A C, Van Loon J J A, Vet Louise M E. Antennal sensilla of two parasitoid wasps: A comparative scanning electron microscopy study. Microscopy Research and Technique, 2004, 63: 266-273.

[17] Groba H F, de Cidre L S L, Castelo M K. Description of antennal structures of the parasitoid Mallophora ruficauda (Diptera: Asilidae) and its relationship with resources searching behavior. Zoomorphology, 2014, 133: 191-204.

[18] 那杰, 于维熙, 李玉萍, 董鑫, 焦娇. 昆虫触角感器的种类及其生理生态学意义. 沈阳师范大学学报: 自然科学版, 2008, 26(2): 214-216.

Na J, Yu W X, Li Y P, Dong X, Jiao J. Types and physiological ecology significance of insects antenna sensilla. Journal of Shenyang Normal University: Natural Science Edition, 2008, 26(2): 214-216. (in Chinese)

[19] Ochieng S A, Park K C, Zhu J W, Baker T C. Functional morphology of antennal chemoreceptors of the parasitoid Microplitis croceipes (Hymenoptera: Braconidae). Arthropod Structure & Development, 2000, 29: 231-240.

[20] Zacharuk R Y. Ultrastructure and function of insect chemosensilla. Annual Review of Entomology, 1980, 25: 27-47.

[21] Almaas T, Mustaparta H. Heliothis virescens: Response characteristics of receptor neurons in sensilla trichodea type1 and type2. Journal of Chemical Ecology, 1991, 17(5): 953-972.

[22] 吴才宏. 棉铃虫雄蛾触角的毛形感器对其性信息素组分及类似物的反应. 昆虫学报, 1993, 36(4): 385-389.

Wu C H. Responses from sensilla on the antennae of male Heliothis armigera to its sex pheromone components and analogs. Acta Entomologica Sinica, 1993, 36(4): 385-389. (in Chinese)

[23] 金鑫, 张善干, 张龙. 东亚飞蝗四种类型的触角感受器超微结构 (昆虫纲: 直翅目). 农业生物技术学报, 2004, 12(3): 300-305.

Jin X, Zhang S G, Zhang L. Ultrastructure of four types of antennal sensilla in Locusta migratoria manilensis (Insecta: Orthoptera). Journal of Agricultural Biotechnology, 2004, 12(3): 300-305. (in Chinese)

[24] 高宇, 王志英, 赵红盈, 刘欣. 白蜡吉丁啮小蜂触角感觉器的扫描电镜观察. 中国农业科学, 2013, 46(9): 1956-1964.

Gao Y, Wang Z Y, Zhao H Y, Liu X. Scanning electron microscopy observation on the antennal sensilla of Tetrastichus planipennisi (Hymenoptera: Eulophidae). Scientia Agricultura Sinica, 2013, 46(9): 1956-1964. (in Chinese)

[25] 李坤, 罗梅浩, 赵国强, 刘晓光. 烟实夜蛾 (Helicoverpa assulta Guenée)触角感器的超微结构观察. 河南农业大学学报, 2006, 40(3): 250-253.

Li K, Luo M H, Zhao G Q, Liu X G. Observation on the ultrastructures of antennal sensilla in Helicoverpa assulta. Journal of Henan Agricultural University, 2006, 40(3): 250-253. (in Chinese)

[26] 李科明, 张永军, 吴孔明, 郭予元. 中红侧沟茧蜂触角超微结构. 中国农业科学, 2012, 45(17): 3522-3530.

Li K M, Zhang Y J, Wu K M, Guo Y Y. Antenna ultrastructure of Microplitis mediator. Scientia Agricultura Sinica, 2012, 45(17): 3522-3530. (in Chinese)

[27] Tarumingkeng R C, Coppel H C, Matsumura F.Morphology and ultrastructure of the antennal chemoreceptors and mechanorecepteors of worker Coptotermes formosanus Shiraki. Cell and Tissue Research, 1976, 173: 173-178.

[28] 杜永均, 严福顺, 唐觉. 大豆蚜触角嗅觉感器结构及其功能. 昆虫学报, 1995, 38(1): 1-7.

Du Y J, Yan F S, Tang J. Structure and function of olfactory sensilla on the antennae of soybean aphids, Aphis glycines. Acta Entomologica Sinica, 1995, 38(1): 1-7. (in Chinese)

[29] 赵红盈, 王志英, 高宇. 密点曲姬蜂触角感觉器的扫描电镜观察. 中国农业科学, 2012, 45(15): 3213-3221.

Zhao H Y, Wang Z Y, Gao Y. Antennal morphology and sensilla distribution of Scambus punctatus (Hymenoptera: Ichneumonidae) with scanning electron microscopy observation. Scientia Agricultura Sinica, 2012, 45(15): 3213-3221. (in Chinese)

[30] 陆宴辉, 仝亚娟, 吴孔明. 绿盲蝽触角感器的扫描电镜观察. 昆虫学报, 2007, 50(8): 863-867.

Lu Y H, Tong Y J, Wu K M. Antennal sensilla of the green plant bug Lygus lucorum Meyer-Dǜr (Heteroptera: Miridae) observed with scanning electron microscopy. Acta Entomologica Sinica, 2007, 50(8): 863-867. (in Chinese)

[31] Onagbola E O, Raj Boina D, Hermann S L, Stelinski L L. Antennal sensilla of Tamarixia radiata (Hymenoptera: Eulophidae), a parasitoid of Diaphorina citri (Hemiptera: Psyllidae). Annals of the Entomological Society of America, 2009, 102(3): 523-531.

[32] Onagbola E O, Fadamiro H Y.Scanning electron microscopy studies of antennal sensilla of Pteromalus cerealellae (Hymenoptera: Pteromalidae). Micron, 2008, 39: 526-535.

[33] Gao Y, Luo L L, Hammond A. Antennal morphology, structure and sensilla distribution in Microplitis pallidipes (Hymenoptera: Braconidae). Micron, 2007, 38: 684-693.

[34] 高其康, 胡萃. 野蚕黑卵蜂触角感器的超微结构研究.浙江农业大学学报, 1993, 19(4): 399-404.

Gao Q K, Hu C. Ultrastructural studies on the antennal sensilla of Telenomus theophilae Wu et Chen (Hymenoptera: Scelionidae). Journal of Zhejiang Agricultural University, 1993, 19(4): 399-404. (in Chinese)

[35] Dweck H K M. Antennal sensory receptors of Pterolamu spuparum female (Hymenoptera: Pteromalidae), a gregarious pupal endoparasitoid of Pieris rapae. Micron, 2009, 40: 769-774.

[36] 邓小刚. 烟蚜茧蜂—规模繁殖与应用. 北京: 中国环境科学出版社, 2010: 59-137.

Deng X G. Aphidius gifuensis Mass Rearing and Application. Beijing: China Environmental Science Press, 2010: 59-137. (in Chinese)

[37] Bourdais D, Vernon P, Krespi L, Le Lannic J, Van Baaren J. Antennal structure of male and female Aphidius rhopalosiphi DeStefani-Peres (Hymenoptera: Braconidae): description and morphological alterations after cold storage or heat exposure. Microscopy Research and Techniques, 2006, 69: 1005-1013.

[38] Amice G, Vernon P, Outerman Y, Van Alphen J, Van Baaren J. Variability in responses to thermal stress in parasitoids. Ecological Entomology, 2008, 33: 701-708.

[39] Rinehart J, Yocum G, Denlinger D. Thermotolerance and rapid cold hardening ameliorate the negative effects of brief exposures to high or low temperatures on fecundity in the flesh fly, Sarcophaga crassipalpis. Physiological Entomology, 2000, 25: 330-336.

[40] David J R, Araripe L O, Chakir M, Legout H, Lemos B, Pétavy G, Rohmer C, Joly D, Moreteau B. Male sterility at extreme temperatures: a significant but neglected phenomenon for understanding Drosophila climatic adaptations. Journal of Evolutionary Biology, 2005, 18: 838-846.

[41] Hochachka P W, Somero G N. Biochemical Adaptation: Mechanism and Process in Physiological Evolution. Oxford: Oxford University Press, 2002.

[42] Bourdais D, Vernon P, Krespi L, Van Baaren J. Behavioural consequences of cold exposure on males and females of Aphidius rhopalosiphi De Stephani Perez (Hymenoptera: Braconidae). BioControl, 2012, 57: 349-360.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Antennal Ultrastructure of Aphidius gifuensis and the Effect of Cold Storage on Antennae

RichHTML

PDF

Abstract

Cite this article

share this article

References

Related Articles 8

Metrics

Comments

Recommended 0

[1]	LÜ XinNing,WANG Yue,JIA RunPu,WANG ShengNan,YAO YuXin. Effects of Melatonin Treatment on Quality of Stored Shine Muscat Grapes Under Different Storage Temperatures [J]. Scientia Agricultura Sinica, 2022, 55(7): 1411-1422.
[2]	LIU Nian, SUN Yan-yan, BAI Hao, HUA Deng-ke, XUE Fu-guang, LIU Ran-ran, LI Dong-li, WEN Jie, CHEN Ji-lan . iTRAQ-Based Proteomic Analysis for Identification of Candidate Proteins Underlying Beak Deformity in Chickens [J]. Scientia Agricultura Sinica, 2015, 48(21): 4390-4396.
[3]	ZHAO Jing, WANG Su, GUO Xiao-Jun, GAO Xi-Wu, ZHANG Fan. Progress in Research of Cold Storage of Insect Parasitoids [J]. Scientia Agricultura Sinica, 2014, 47(3): 482-494.
[4]	GAO Yu, WANG Zhi-Ying, ZHAO Hong-Ying, LIU Xin. Scanning Electron Microscopy Observation on the Antennal Sensilla of Tetrastichus planipennisi (Hymenoptera: Eulophidae) [J]. Scientia Agricultura Sinica, 2013, 46(9): 1956-1964.
[5]	ZHENG Yuan-Rong, LIU Zhen-Min, MO Bei-Hong, GAO Hong-Yan, SUN Ke-Jie. Effects of Microstructure Change on the Texture of Cheddar Cheese During Ripening [J]. Scientia Agricultura Sinica, 2012, 45(3): 503-508.
[6]	ZHAO Hong-Ying, WANG Zhi-Ying, GAO Yu. Antennal Morphology and Sensilla Distribution of Scambus punctatus (Hymenoptera: Ichneunmonidae) with Scanning Electron Microscopy Observation [J]. Scientia Agricultura Sinica, 2012, 45(15): 3213-3221.
[7]	. Effects of Harvest Maturity on Quality and Chilling Injury of Juicy Peaches During Low Temperature Storage [J]. Scientia Agricultura Sinica, 2009, 42(2): 612-618 .
[8]	,. Study on Purifying Technology of Andrographolide by Supercritical Carbon Dioxide Secondary Gradient Crystallization [J]. Scientia Agricultura Sinica, 2005, 38(01): 140-144 .