利用iTRAQ蛋白质组技术筛选与鸡喙畸形相关的候选蛋白

doi:10.3864/j.issn.0578-1752.2015.21.019

Abstract

Abstract: 【Objective】Beak deformity has been reported in some indigenous chickens like Beijing-You chickens and Qingyuan partridge chickens with deformed beaks have problems with eating and drinking resulting in obvious reductions of growth and production performances. Observations on pedigreed individuals with deformed beaks shed light on the heritability of this trait. The aim of this study was to identify the candidate proteins related to beak deformity using isobaric tags for relative and absolute quantitation (iTRAQ) technology and bioinformatic analysis. The results can build the basis for further understanding the mechanism of beak deformity in chickens. 【Method】Three chickens with deformed beaks (W1, W2 and W3) and their sibs (Z1, Z2 and Z3) of 120 days of age were used in this study. Beak sample proteins of these chickens were collected by using tissue total protein lysis buffer. Six iTRAQs were used to label samples of three contrast groups (W1 vs Z1, W2 vs Z2, and W3 vs Z3). After separation by chromatography and analysis of liquid chromatography tandem mass spectrometry, differentially expressed proteins were identified and quantitatively analyzed by Mascot 2.3.02 software. Comparing deformed samples with the normal samples, we chose some differentially expressed proteins which consisted of 2 or more peptides, P＜0.05, and the ratio of which was more than 1.2 (up-regulated) or less than 0.83 (down-regulated). 【Result】 In total, 3 372 proteins were detected from three contrast groups using iTRAQ and 1 869 of them were Jungle fowl proteins. Of these, 159 were differentially expressed proteins, 70 were up-regulated and 89 were down-regulated. The most up-regulated proteins included LPL, MLC-2, CO9A1, MATN3, and HSP90B1, while the most down-regulated proteins were MBP, RLA1, PRVM, and HAPLN1. Protein CO9A1, MATN3, and HAPLN1 were associated with cartilage synthesis and ossification. PRVM is an intracellular calcium binding protein involved in regulating the Ca²⁺ ion signaling pathway. LPL is a multifunctional enzyme and mainly plays a role in lipid metabolism and transport, which involves regulating PPAR signaling pathways. 【Conclusion】 Proteins CO9A1, MATN3, HAPLN1, PRVM, and LPL were proposed as candidate proteins for beak deformity. The discovery and identification of these proteins can offer a valuable insights into basis of protein level of beak deformity in chickens.

Key words: Beijing-You chickens, beak deformity, iTRAQ, differentially expressed proteins

LIU Nian, SUN Yan-yan, BAI Hao, HUA Deng-ke, XUE Fu-guang, LIU Ran-ran, LI Dong-li, WEN Jie, CHEN Ji-lan . iTRAQ-Based Proteomic Analysis for Identification of Candidate Proteins Underlying Beak Deformity in Chickens[J].Scientia Agricultura Sinica, 2015, 48(21): 4390-4396.

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