肺形侧耳高温后恢复期间海藻糖代谢途径研究

doi:10.3864/j.issn.0578-1752.2013.24.013

Abstract

Abstract: 【Objective】 The objective of this study is to explore the recovery mechanism after heat response stress of mushroom, in order to provide a theoretical basis for breeding. 【Method】 The responses of trehalose metabolism at the level of enzyme activity and gene relative expression quantity during heat stress recovery period in two strains of Pleurotus pulmonarius, including the heat sensitive strain CCMSSC 00494 and heat tolerant strain CCMSSC 00499 were investigated. 【Result】The results showed that the trehalose content of two strains rapidly decreased to the control level under heat stress recovery conditions, and the rate of decrease in heat sensitive strain CCMSSC 00494 was faster than that in CCMSSC 00499. During post-heat stress recovery period, trehalose-6-phosphate synthase (TPS) activity of CCMSSC 00494 increased with prolongation of treatment time, while it decreased slowly in CCMSSC 00499. And in both strains, trehalose phosphorylase (TP) activity in the direction of trehalose synthesis decreased suddenly, and TP activity in the direction of trehalose phosphorolysis and neutral trehalase (NTH) activity for trehalose hydrolysis were activated in the early and late period of recovery, respectively. In contrast with the case of tps and tp Genes, the increase of nth mRNA expression was not consistent with the elevation of neutral trehalase activity during heat stress in both strains. 【Conclusion】The main difference between heat sensitive and heat tolerant strains is that the activities and mRNA expression levels of TPS in heat sensitive strain was significantly higher during heat stress recovery period.

Key words: Pleurotus pulmonarius , trehalose , heat stress recovery , heat resistance

LIU Xiu-Ming, HUANG Chen-Yang, CHEN Qiang, WU Xiang-Li, ZHANG Jin-Xia. Study on the Metabolic Pathway of Trehalose in Pleurotus pulmonarius During Heat Stress Recovery[J].Scientia Agricultura Sinica, 2013, 46(24): 5188-5195.

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Study on the Metabolic Pathway of Trehalose in Pleurotus pulmonarius During Heat Stress Recovery

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