Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (21): 4569-4577.doi: 10.3864/j.issn.0578-1752.2013.21.020

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Optimization of Ultrasound-Assisted Extraction Technology of Polyphenols from Apple Pomace by Response Surface Methodology

 LI  Zhen, HA  Yi-Ming, LI  An, LI  Wei-Ming, WANG  Feng, LI  Qing-Peng   

  1. Institute of Agro-food Science & Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-product Processing, Ministry of Agriculture, Beijing 100193
  • Received:2013-02-01 Online:2013-11-01 Published:2013-08-21

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Abstract: 【Objective】The objective of this study is to optimize ultrasound-assisted ethanol extraction of polyphenols from apple pomace. 【Method】Residues from juice extraction of apple were used in this paper. On the basis of single-factor test, the response surface methodology was used to investigate the effects of processing parameters, including extraction time, temperature, ultrasound power and solvent/material ratio. The extraction rate of polyphenols was chosen as response value, mathematical model was established by implementing four factors and three levels. 【Result】 The model was of great difference, and the established regression equation for polyphenols yield had an excellent goodness of fit. Therefore the polyphenols from apple pomance could be analyzed and predicted by the model. Factors influencing the extraction of polyphenols from the apple pomace were in the order as follows: extraction temperature＞solvent/material ratio＞ultrasound power＞extraction time. The response surface plots showed that the interaction between extraction time and ultrasound power was not significant, while the interaction between extraction temperature and ultrasound power was outstanding, and the main effect of solvent/material ratio was greater than the temperature. The optimum technological conditions were extraction time of 10 min, extraction temperature of 65℃, ultrasonic power of 503 W, solvent/material ratio of 1﹕30(g•mL-1).【Conclusion】The extraction ratio of apple polyphenols was up to 4.53 mg•g-1, which is highly consistent with the predictive value of 4.55 mg•g-1.

Key words: apple pomace , polyphenols , ultrasound assisted , response surface methodology

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