Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (14): 3046-3054.doi: 10.3864/j.issn.0578-1752.2013.14.021

• RESEARCH NOTES • Previous Articles     Next Articles

Differentially Expressed Protein Profile of Maize Seedling Leaves Between Hybrid and Its Parental Lines

 GUO  Bao-Jian, SUI  Zhi-Peng, LI  Yang-Yang, FENG  Wan-Jun, YAN  Wen-Wen, LI  Hui-Min, SUN  Qi-Xin, NI  Zhong-Fu   

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University / National Plant Gene Research Centre (Beijing)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education / Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193
  • Received:2013-02-21 Online:2013-07-15 Published:2013-04-16

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Abstract: 【Objective】To construct protein expression profile of seedling leaves between maize hybrid and its parental lines, with the purpose to give an insight into the molecular basis of leaf size heterosis. 【Method】 Differentially expressed protein profile of the third leaf on 5th day after germination between maize hybrid and its parental lines used for 2-DE analysis and differentially expressed proteins were identified by using MALDI TOF MS method. 【Result】 A total of 630 protein spots were detected, among which 52 protein spots were found to be differentially expressed between maize hybrid and its parental lines. Differentially expressed protein spots could be grouped into six models, that is UPF1 (expression in hybrid and uniparent but not in another parent), HDH(hybrid is equal to the highly expressed parent), LDH (hybrid is equal to the lowly expressed parent), URH (up-regulated in hybrid), DRH (down-regulated in hybrid), F1nBP (hybrid-specific expressed protein spots), and the numbers were 15, 13, 8, 6, 7 and 3, respectively. Moreover, 28 of the 52 differentially expressed protein spots were identified by using MALDI TOF MS, which were grouped into nine functional categories, including metabolism, development, stress, glycolysis, transcription regulation, protein folding and degradation, tricarboxylic acid cycle, cytoskeleton and unknown protein. 【Conclusion】 Significant alterations in protein expression occurred in seedling leaves of hybrids and parental lines and differentially expressed proteins implicated in some functional categories might contribute to heterosis related to leaf size heterosis.

Key words: maize , leaf size , heterosis , differentially expressed protein , molecular mechanism

[1]Swanson-Wagner R A, Jia Y, DeCook R, Borsuk L A, Nettleton D, Schnable P S. All possible modes of gene action are observed in a global comparison of gene expression in a maize F1 hybrid and its inbred parents. Proceedings of the National Academy of Sciences of the USA, 2006, 103(18): 6805-6810.

[2]Song S H, Qu H Z, Chen C, Hu S N, Yu J. Differential gene expression in an elite hybrid rice cultivar (Oryza sativa L.) and its parental lines based on SAGE data. BMC Plant Biology, 2007, 7: 49.

[3]Meyer R C, Kusterer B, Lisec J, Steinfath M, Becher M, Scharr H, Melchinger A E, Selbig J, Schurr U, Willmitzer L, Altmann T. QTL analysis of early stage heterosis for biomass in Arabidopsis. Theoretical and Applied Genetics, 2010, 120(2): 227-237.

[4]孙其信. 农作物杂种优势机理研究及展望. 作物杂志, 1998, 4: 31.

Sun Q X. Crop heterosis mechanism and prospects. Crop Journal, 1998, 4: 31. (in Chinese)

[5]Romagnoli S, Maddaloni M, Livini C, Motto M. Relationship between gene expression and hybrid vigor in primary root tips of young maize (Zea mays L.) plantlets. Theoretical and Applied Genetics, 1990, 80(6): 769-775.

[6]Marcon C, Schützenmeister A, Schütz W, Madlung J, Piepho H P, Hochholdinger F. Nonadditive protein accumulation patterns in maize (Zea mays L.) hybrids during embryo development. Journal of Proteome Research, 2010, 9(12): 6511-6522.

[7]张义荣, 姚颖垠, 彭惠茹, 张庆波, 倪中福, 孙其信. 植物杂种优势形成的分子遗传机理研究进展. 自然科学进展, 2009, 19(7): 697-703.

Zhang Y R, Yao Y Y, Peng H R, Zhang Q B, Ni Z F, Sun Q X. Progress in molecular genetic mechanism of plant heterosis. Progress in Natural Science, 2009, 19(7): 697-703. (in Chinese)

[8]Hoecker N, Keller B, Muthreich N, Chollet D, Descombes P, Piepho H P, Hochholdinger F. Comparison of maize (Zea mays L.) F1-hybrid and parental inbred line primary root transcriptomes suggests organ-specific patterns of nonadditive gene expression and conserved expression trends. Genetics, 2008, 179(3): 1275-1283.

[9]Fu Z Y, Jin X N, Ding D, Li Y L, Fu Z J, Tang J H. Proteomic analysis of heterosis during maize seed germination. Proteomics, 2011, 11: 1462-1472.

[10]Paschold A, Jia Y, Marcon C, Lund S, Larson N B, Yeh C T, Ossowski S, Lanz C, Nettleton D, Schnable P S, Hochholdinger F. Complementation contributes to transcriptome complexity in maize (Zea mays L.) hybrids relative to their inbred parents. Genome Research, 2012, 22(12): 2445-2454.

[11]Hoecker N, Lamkemeyer T, Sarholz B, Paschold A, Fladerer C, Madlung J, Wurster K, Stahl M, Piepho H P, Nordheim A, Hochholdinger F. Analysis of nonadditive protein accumulation in young primary roots of a maize (Zea mays L.) F1-hybrid compared to its parental inbred lines. Proteomics, 2008, 8(18): 3882-3894.

[12]Huang Y, Zhang L D, Zhang J W, Yuan D J, Xu C G , Li X H , Zhou D X , Wang S P, Zhang Q F. Heterosis and polymorphisms of gene expression in an elite rice hybrid as revealed by a microarray analysis of 9198 unique ESTs. Plant Molecular Biology, 2006, 62(4/5): 579-591.

[13]Meyer R C, Witucka-Wall H, Becher M, Blacha A, Boudichevskaia A, Dörmann P, Fiehn O, Friedel S, von Korff M, Lisec J, Melzer M, Repsilber D, Schmidt R, Scholz M, Selbig J, Willmitzer L, Altmann T. Heterosis manifestation during early Arabidopsis seedling development is characterized by intermediate gene expression and enhanced metabolic activity in the hybrids. The Plant Journal, 2012, 71(4): 669-683.

[14]Dahal D, Mooney B P, Newton K J. Specific changes in total and mitochondrial proteomes are associated with higher levels of heterosis in maize hybrids. The Plant Journal, 2012, 72(1): 70-83.

[15]Song X, Ni Z F, Yao Y Y, Zhang Y H, Sun Q X. Identification of differentially expressed proteins between hybrid and parents in wheat (Triticum aestivum L.) seedling leaves. Theoretical and Applied Genetics, 2009, 118(2): 213-225.

[16]Wang W, Meng B, Ge X, Song S, Yang Y, Yu X, Wang L, Hu S,  Liu S, Yu J. Proteomic profiling of rice embryos from a hybrid    rice cultivar and its parental lines. Proteomics, 2008, 8(22): 4808-4821.

[17]Song X, Ni Z F, Yao Y Y, Xie C J, Li Z X, Wu H Y, Zhang Y H, Sun Q X. Wheat (Triticum aestivum L.) root proteome and differentially expressed root proteins between hybrid and parents. Proteomics, 2007, 7(19): 3538-3557.

[18]Ni Z F, Kim E D, Ha M S, Lackey E, Liu J X, Zhang Y R, Sun Q X, Chen Z J. Altered circadian rhythms regulate growth vigour in hybrids and allopolyploids. Nature, 2009, 457: 327-331.

[19]Guo M, Rupe M A, Dieter J A, Zou J, Spielbauer D, Duncan K E, Howard R J, Hou Z, Simmons C R. Cell number regulator1 affects plant and organ size in maize: Implications for crop yield enhancement and heterosis. The Plant Cell, 2010, 22(4): 1057-1073.

[20]Li P, Ponnala L, Gandotra N, Wang L, Si Y, Tausta S L, Kebrom T H, Provart N, Patel R, Myers C R, Reidel E J, Turgeon R, Liu P, Sun Q, Nelson T, Brutnell T P. The developmental dynamics of the maize leaf transcriptome. Nature Genetic, 2010, 42(12): 1060-1067.

[21]Palmer S J. Davies W J. An analysis of relative elemental growth rate, epidermal cell size and xyloglucan endotransglycosylase activity through the growing zone of ageing maize leaves. Journal of Experimental Botany, 1996, 47(296): 339-347.

[22]Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, 72: 248-254.

[23]宋方威, 彭惠茹, 张义荣, 杨小红, 刘婷, 孙其信, 倪中福. 利用三重测交群体剖析玉米苗期性状杂种优势的遗传学基础。农业生物技术学报, 2011, 19(5): 785-792.

Song F W, Peng H R, Zhang Y R, Yang X H, Liu T, Sun Q X, Ni Z F. Heterosis for seeding traits in maize (Zea mays L.) revealed by quantitative trait loci analysis of the triple testcross design. Journal of Agricultural Biotechnology, 2011, 19(5): 785-792. (in Chinese)

[24]Frascaroli E, Canè M A, Landi P, Pea G, Gianfranceschi L, Villa M, Morgante M, Pè M E. Classical genetic and quantitative trait loci analyses of heterosis in a maize hybrid between two elite inbred lines. Genetics, 2007, 176: 625-644.

[25]Lange P R, Geserick C, Tischendorf G, Zrenner R. Functions of chloroplastic adenylate kinases in Arabidopsis. Plant Physiology, 2008, 146(2): 492-504.

[26]Regierer B, Fernie A R, Springer F, Perez-Melis A, Leisse A, Koehl K, Willmitzer L, Geigenberger P, Kossmann J. Starch content and yield increase as a result of altering adenylate pools in transgenic plants. Nature Biotechnology, 2002, 20(12): 1256-1260.

[27]Staiger C J. Signaling to the actin cytoskeleton in plants. Annual Review of Plant Physiology and Plant Molecular Biology, 2000, 51: 257-288.

[28]An Y Q, Huang S, McDowell J M, McKinney E C, Meagher R B. Conserved expression of the Arabidopsis ACT1 and ACT3 actin subclass in organ primordia and mature pollen. The Plant Cell, 1996, 8(1): 15-30.

[29]McDowell J M, An Y Q, Huang S, McKinney E C, Meagher R B. The Arabidopsis ACT7 actin gene is expressed in rapidly developing tissues and responds to several external stimuli. Plant Physiology, 1996, 111: 699-711.

[30]McDowell J M, Huang S, McKinney E C, An Y Q, Meagher R B. Structure and evolution of the actin gene family in Arabidopsis thaliana. Genetics, 1996, 142(2): 587-602.

[31]Kandasamy M K, McKinney E C, Meagher R B. A single vegetative actin isovariant overexpressed under the control of multiple regulatory sequences is sufficient for normal Arabidopsis development. The Plant Cell, 2009, 21(3): 701-718.

[32]Winter D, Vinegar B, Nahal H, Ammar R, Wilson G V, Provart N J. An “electronic fluorescent pictograph” browser for exploring and analyzing large-scale biological data sets. PloS ONE, 2007, 2(8): e718.

[33]Shen H S, He H, Li J G, Chen W, Wang X C, Guo L, Peng Z Y, He G M, Zhong S W, Qi Y J, Terzaghi W, Deng X W. Genome-wide analysis of DNA methylation and gene expression changes in two Arabidopsis ecotypes and their reciprocal hybrids. The Plant Cell, 2012, 24: 875-892.

[34]Johnston A J, Matveeva E, Kirioukhova O, Grossniklaus U, Gruissem W. A dynamic reciprocal RBR-PRC2 regulatory circuit controls Arabidopsis gametophyte development. Current Biology, 2008, 18(21): 1680-1686.
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