Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (14): 2979-2989.doi: 10.3864/j.issn.0578-1752.2013.14.014

• HORTICULTURE • Previous Articles     Next Articles

Spatial and Temporal Accumulation of Flavanols, Activity and Tissue Localization of Leucoanthocyanidin Reductase Induced by Soil Drought in Developing Grape Berries

 WEN  Peng-Fei, NIU  Xing-Yan, XING  Yan-Fu, GAO  Mei-Ying, NIU  Tie-Quan   

  1. College of Horticulture, Shanxi Agricultural University, Taigu 030801, Shanxi
  • Received:2012-12-14 Online:2013-07-15 Published:2013-05-21

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Abstract: 【Objective】The effects of soil drought on the spatial and temporal accumulation of flavanols, enzyme activity and tissue localization of its biosynthetic key enzyme, leucoanthocyanidin reductase (LAR), during the grape berry development were studied.【Method】The 5-year old grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) was used as materials, and the grapevine was subjected to soil drought stress simulated by controlling irrigation through the rain-preventing shelter and root-cutting groove during grape berry development. The spatial and temporal accumulation of flavanols and the enzyme activity of LAR in different parts of the grape berry were analyzed by spectrophotometer method, and the tissue localization of LAR was detected by the immunohistochemical localization. 【Result】 Results indicated that the growth of grape berry was significantly inhibited by soil drought during its development, and an obvious accumulation of total phenol in berry was induced by soil drought, especially in the young berry period. The accumulation pattern of flavanols and total flavan-3-ols in skin and flesh during grape berry development were not changed by soil drought, whereas an obvious accumulation of flavanols and total flavan-3-ols induced by soil drought was observed, and this effect was organ- and development-dependent. The LAR enzyme activity in skin and flesh was induced by soil drought, especially in the young berry period. There were no obvious changes of tissue localization of LAR1 and LAR2 in the skin and flesh under soil drought, but an increasing signal was detected, especially in the vascular bundle.【Conclusion】All the results of this study suggested that soil drought could induce the accumulation of LAR1 and LAR2 proteins in different parts of berry at different development stages, resulted in the increase of LAR enzyme activity in corresponding part and developing stage, and induced the accumulation of flavanols and total flavan-3-ols during grape berry development.

Key words: grape berry , soil drought , flavanols , LAR , tissue localization

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