Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (11): 2240-2248.doi: 10.3864/j.issn.0578-1752.2013.11.007

• PLANT PROTECTION • Previous Articles     Next Articles

Enzymatic Characteristics of 3-Acetyl Deoxynivalenol Oxidase by Devosia sp. DDS-1

 XU  Jian-Hong, PAN  Yan-Mei, HU  Xiao-Dan, JI  Fang, YIN  Xian-Chao, SHI  Jian-Rong   

  1. 1.Institute of Safety and Detection of Food Quality, Jiangsu Academy of Agricultural Sciences/Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base/Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality , Ministry of Agriculture/Jiangsu Center for GMO Evaluation and Detection, Nanjing 210014
    2.Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095
  • Received:2012-12-04 Online:2013-06-01 Published:2013-04-01

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Abstract: 【Objective】 The objective of this study is to investigate the enzymatic characteristics of 3-acetyl deoxynivalenol oxidase (3-AC-DON oxidase), understand the degradation characteristics of 3-acetyl deoxynivalenol by Devosia sp. DDS-1, and realize the application of the enzyme.【Method】After being cultured in LG medium , the strain DDS-1 was centrifuged and washed by PBS, and then broken by ultrasonic treatment. The crude enzyme of DDS-1 was obtained by ammonium sulfate precipitation. The enzymatic activity of 3-AC-DON oxidase was then detected in different enzyme reaction systems. 【Result】 The optimal 3-AC-DON oxidase production conditions for DDS-1 were 25℃, pH 7.0 and 36 hours in LG medium. The optimum reaction conditions for the 3-AC-DON oxidase in DDS-1 were 35℃, pH 7.0. The enzymatic activity could be maintained above 80% from 20 to 40℃ and in the range of pH 5.0 to 9.0. Most metal ions at a concentration from 0.2 to 2 mmol•L-1 could promote the enzymatic activity. Ethylenediamine tetraacetic acid (EDTA) inhibited the enzyme activity on the contrary. The crude enzyme produced by DDS-1 could degrade DON, 3-AC-DON in wheat.【Conclusion】The enzymatic characteristics of 3-AC-DON oxidase produced by Devosia sp. DDS-1 was studied in this paper. The results showed that Devosia sp. DDS-1 can produce 3-AC-DON oxidase with good temperature and pH stability, and the enzyme requires metal ions as cofactor.

Key words: 3-acetyl deoxynivalenol , degradation , Devosia sp. , enzymatic activity

[1]Jaksic S, Abramovic B, Jajic I, Balos M Z, Mihaljev Z, Despotovic V, Sojic D. Co-occurrence of fumonisins and deoxynivalenol in wheat and maize harvested in Serbia. Bulletin of Environmental Contamination and Toxicology, 2012, 89(3): 615-619.

[2]van der Fels-Klerx H J, de Rijk T C, Booij C J, Goedhart P W, Boers E A M, Zhao C, Waalwijk C, Mol H G J, van der Lee T A J. Occurrence of Fusarium head blight species and Fusarium mycotoxins in winter wheat in the Netherlands in 2009. Food Additives & Contaminants: Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 2012, 29(11): 1716-1726.

[3]Lee T, Lee S H, Lee S H, Shin J Y, Yun J C, Lee Y W, Ryu J G. Occurrence of Fusarium mycotoxins in rice and its milling by-products in Korea. Journal of Food Protection, 2011, 74(7): 1169-1174.

[4]Mylona K, Sulyok M, Magan N. Relationship between environmental factors, dry matter loss and mycotoxin levels in stored wheat and maize infected with Fusarium species. Food Additives & Contaminants: Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 2012, 29(7): 1118-1128.

[5]Edwards S G, Dickin E T, MacDonald S, Buttler D, Hazel C M, Patel S, Scudamore K A. Distribution of Fusarium mycotoxins in UK wheat mill fractions. Food Additives & Contaminants: Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 2011, 28(12): 1694-1704.

[6]Bernhoft A, Torp M, Clasen P E, Loes A K, Kristoffersen A B. Influence of agronomic and climatic factors on Fusarium infestation and mycotoxin contamination of cereals in Norway. Food Additives & Contaminants: Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 2012, 29(7): 1129-1140.

[7]Zhang H, Van der Lee T, Waalwijk C, Chen W, Xu J, Xu J S, Zhang Y, Feng J. Population analysis of the Fusarium graminearum species complex from wheat in China show a shift to more aggressive isolates. PLoS One, 2012, 7(2): e31722.

[8]Zhang X, Zhou M, Ren L, Bai G, Ma H, Scholten O E, Guo P G, Lu W Z. Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai. Euphytica, 2004, 139(1): 59-64.

[9]张旭, 邢锦城, 马鸿翔, 温云平, 陆维忠, 袁生. 江淮流域小麦赤霉病菌的遗传多样性. 江西农业大学学报, 2010, 32(6): 1146-1151.

Zhang X, Xing J C, Ma H X, Wen Y P, Lu W Z, Yuan S. Genetic diversity of Fusarium spp. isolates in Yangtze-huaihe river valley. Acta Agriculturae Universitis Jiangxiensis, 2010, 32(6): 1146-1151. (in Chinese)

[10]马鸿翔, 陆维忠. 小麦赤霉病抗性改良研究进展. 江苏农业学报, 2010, 26(1): 197-203.

Ma H X, Lu W Z. Progress on genetic improvement for resistance to Fusarium head blight in wheat. Jiangsu Journal of Agricultural Sciences, 2010, 26(1): 197-203. (in Chinese)

[11]Piekkola S, Turner P C, Abdel-Hamid M, Ezzat S, El-Daly M, El-Kafrawy S, Savchenko E, Poussa T, Woo J C S, Mykkanen H, El-Nezami H. Characterisation of aflatoxin and deoxynivalenol exposure among pregnant Egyptian women. Food Additives & Contaminants: Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 2012, 29(6): 962-971.

[12]Ruiz M J, Franzova P, Juan-Garcia A, Font G. Toxicological interactions between the mycotoxins beauvericin, deoxynivalenol and T-2 toxin in CHO-K1 cells in vitro. Toxicon, 2011, 58(4): 315-326.

[13]Barros G, Alaniz Zanon M S, Palazzini J M, Haidukowski M, Pascale M, Chulze S. Trichothecenes and zearalenone production by Fusarium equiseti and Fusarium semitectum species isolated from Argentinean soybean. Food Additives & Contaminants: Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 2012, 29(9): 1436-1442.

[14]Almeida M I, Almeida N G, Carvalho K L, Goncalves G A, Silva C  N, Santos E A, Garcia J C, Vargas E A. Co-occurrence of aflatoxins B1, B2, G1 and G2, ochratoxin A, zearalenone, deoxynivalenol, and citreoviridin in rice in Brazil. Food Additives & Contaminants: Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 2012, 29(4): 694-703.

[15]Zhang Y, Caupert J. Survey of mycotoxins in U.S. distiller's dried grains with solubles from 2009 to 2011. Journal of Agricultural and Food Chemistry, 2012, 60(2): 539-543.

[16]Awad W A, Ghareeb K, Bohm J, Zentek J. Decontamination and detoxification strategies for the Fusarium mycotoxin deoxynivalenol in animal feed and the effectiveness of microbial biodegradation. Food Additives & Contaminants: Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 2010, 27(4): 510-520.

[17]Bejaoui H, Mathieu F, Taillandier P, Lebrihi A. Biodegradation of ochratoxin A by Aspergillus section Nigri species isolated from French grapes: a potential means of ochratoxin A decontamination in grape juices and musts. FEMS Microbiology Letters, 2006, 255(2): 203-208.

[18]Seeling K, Boguhn J, Strobel E, Danicke S, Valenta H, Ueberschar K H, Rodehutscord M. On the effects of Fusarium toxin contaminated wheat and wheat chaff on nutrient utilisation and turnover of deoxynivalenol and zearalenone in vitro (Rusitec). Toxicology in Vitro, 2006, 20(5): 703-711.

[19]Fuchs E, Binder E M, Heidler D, Krska R. Structural characterization of metabolites after the microbial degradation of type A trichothecenes by the bacterial strain BBSH 797. Food Additives and Contaminants, 2002, 19(4): 379-386.

[20]Shima J, Takase S, Takahashi Y, Iwai Y, Fujimoto H, Yamazaki M, Ochi K. Novel detoxification of the trichothecene mycotoxin deoxynivalenol by a soil bacterium isolated by enrichment culture. Applied and Environmental Microbiology, 1997, 63(10): 3825-3830.

[21]He C, Fan Y, Liu G, Zhang H. Isolation and identification of a strain of Aspergillus tubingensis with deoxynivalenol biotransformation capability. International Journal of Molecular Sciences, 2008, 9(12): 2366-2375.

[22]Ikunaga Y, Sato I, Grond S, Numaziri N, Yoshida S, Yamaya H, Hiradate S, Hasegawa M, Toshima H, Koitabashi M, Ito M, Karlovsky P, Tsushima S. Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol. Applied Microbiology and Biotechnology, 2011, 89(2): 419-427.

[23]Sato I, Ito M, Ishizaka M, Ikunaga Y, Sato Y, Yoshida S, Koitabashi M, Tsushima S. Thirteen novel deoxynivalenol-degrading bacteria are classified within two genera with distinct degradation mechanisms. FEMS Microbiology Letters, 2012, 327(2): 110-117.

[24]徐剑宏, 祭芳, 王宏杰, 王建伟, 林凡云, 史建荣. 脱氧雪腐镰刀菌烯醇降解菌的分离和鉴定. 中国农业科学, 2010, 43(22): 4635-4641.

Xu J H, Ji F, Wang H J, Wang J W, Lin F Y, Shi J R. Isolation and identification of deoxynivalenol degradation strains. Scientia Agricultura Sinica, 2010, 43(22): 4635-4641. (in Chinese)

[25]张忠辉, 洪青, 张国顺, 徐剑宏, 李顺鹏. 杀螟硫磷降解菌FDS-1的分离鉴定及其降解特性. 中国环境科学, 2005, 25(1): 52-56.

Zhang Z H, Hong Q, Zhang G S, Xu J H, Li S P. Studied on isolation identification of fenitrothion degradation bacteria FDS-1 and its degradation characteristics. China Environmental Science, 2005, 25(1): 52-56. (in Chinese)

[26]Azcona-Olivera J I, Ouyang Y L, Warner R L, Linz J E , Pestka J J. Effects of vomitoxin (deoxynivalenol) and cycloheximide on IL-2, 4, 5 and 6 secretion and mRNA levels in murine CD4+ cells. Food and Chemical Toxicology, 1995, 33(6): 433-441.
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