Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (24): 5110-5116.doi: 10.3864/j.issn.0578-1752.2012.24.017

• VETERINARY SCIENCE • Previous Articles    

The Regulation of Autoinducer-2 in Avian Pathogenic Escherichia coli

 BAI  Hao, HAN  Xian-Gan, LIU  Lei, DAN  Xue-Qin, SONG  Jun, LIU  Rui, DONG  Hong-Liang, LIU  Hai-Wen, DING  Chan, YU  Sheng-Qing   

  1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241
  • Received:2012-10-26 Online:2012-12-15 Published:2012-12-14

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Abstract: 【Objective】 The aim of the study was to investigate the modulation of AI-2 on the biofilm forming ability and virulence in Avian Pathogenic Escherichia coli (APEC). 【Method】 The improved crystal violet seme-quantitative method and fluorescence staining method were used to study the effects of AI-2 on the biofilm forming ability in APEC. The effects of AI-2 on the mRNA levels of the virulence genes were analyzed using real-time PCR. Viable bacteria counting method was used to evaluate the effects of AI-2 on the capability of APEC to adhere and invade DF-1cell. 【Result】The results showed that the biofilm forming ability decreased at the concentration of 0.185 mmol•L-1 AI-2,while the biofilm forming ability had no significant change at the concentration of 0.037mmol•L-1 and 0.278 mmol•L-1 AI-2. Real-time PCR showed that Al-2 decreased the transcription of pfs, vat, lux, tsh, fuyA, iucD genes, while increased the transcription of ompA and iss genes. The adherence and invasion was decreased to 57.35% and 36.64% by supplementation with AI-2 in APEC. 【Conclusion】The biofilm forming ability increased at an optimal concentration of AI-2. The transcription of the virulence genes, the adherence and invasion to DF-1 cells of the bacteria were decreased by supplementation with AI-2 in the medium to culture the APEC. These findings will be of benefit to future studies of the role of AI-2 in APEC.

Key words: Avian Pathogenic Escherichia coli , AI-2 , biofilm , virulence genes , adherence , invasion

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