Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (10): 2099-2106.doi: 10.3864/j.issn.0578-1752.2012.10.024

• RESEARCH NOTES • Previous Articles    

Rhizosphere Soil Microbial Quantity and Enzyme Activity of Different Apple Rootstocks

 CHEN  Ru, WANG  Hai-Ning, JIANG  Yuan-Mao, WEI  Shao-Chong, CHEN  Qian, GE  Shun-Feng   

  1. 山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东泰安 271018
  • Received:2011-11-22 Online:2012-05-15 Published:2012-02-07

Abstract: 【Objective】The aim of the experiment is to investigate the effects of different apple rootstocks on soil microbial quantity and enzyme activities.【Method】Using microbial dilution plate counting cultivation method, the rhizosphere soil microbial quantity and enzyme activity and their relations of five kinds of apple rootstocks (M. hupehensis Rehd., M. micromalus Makino, M. prunifolia(Willd) Borkh., M. sievesii(Ledeb.) Roemer and M. baccata Borkh.) were analyzed in a short-term pot experiment. 【Result】The number of microbial physiological groups in rhizosphere soil under different varieties of apple rootstocks were significantly differences, and bacteria were dominant, followed by actinomycetes, and fungi were the least. The total amounts of microoriganisms and biodiversity indices were different among apple rootstocks. Different varieties of apple rootstocks rhizosphere soil invertase, phosphatase, urease and catalase activity varied. Pearson correlation analysis indicated that certain correlation existed between soil microbial amount and soil enzyme activities.【Conclusion】Choosing right apple rootstock varieties can be beneficial to increase the number of soil microorganisms and enzyme activities, and create a good micro-ecological environment for the growth of apple trees.

Key words: apple rootstock, rhizosphere soil, microbial quantity, enzyme activities

[1]李天忠, 张志宏. 现代果树生物学. 北京: 科学出版社, 2008: 130-133,157-159, 290-295.

Li T Z, Zhang Z H. Modern Fruit Biology. Beijing: Science Press, 2008: 130-133,157-159, 290-295. (in Chinese)

[2]Nannipieri P, Ascher J, Ceccherini M T, Landi L, Pietramellara G, Renella G. Microbial diversity and soil functions. European Journal of Soil Science, 2003, 54(4), 655-670.

[3]Rajkumar M, Ae N, Prasad M N, Freitas H. Potential of siderophore-producing bacteria for improving heavy metal phytoextraction. Trends in Biotechnology, 2010, 28(3):142-149.

[4]Floch C, Capowiez Y, Criquet S. Enzyme activities in apple orchard agroecosystems: How are they affected by management strategy and soil properties. Soil Biology and Biochemistry, 2009, 41(1): 61-68.

[5]和文祥, 谭向平, 王旭东, 唐  明, 郝明德. 土壤总体酶活性指标的初步研究. 土壤学报, 2010, 7(6): 211-215.

He W X,Tan X P, Wang X D, Tang M, Hao M D. Study on total enzyme activity index in soils. Acta Pedologica Sinica, 2010, 7(6): 211-215. (in Chinese)

[6]曹  慧, 孙  辉, 杨  浩, 孙  波, 赵其国. 土壤酶活性及其对土壤质量的指示研究进展. 应用与环境生物学报, 2003, 9(1): 105-109.

Cao H, Sun H, Yang H, Sun B, Zhao Q G. A review: soil enzyme activity and its indication for soil quality. Chinese Journal of Applied & Environmental Biology, 2003, 9(1): 105-109. (in Chinese)

[7]Wei X D, Zou H L, Chu L M, Liao B, Ye C M, Lan C Y. Field released transgenic papaya effect on soil microbial communities and enzyme activities. Journal of Environmental Sciences, 2006, 18(4): 734-740.

[8]惠竹梅, 岳泰新, 张  瑾, 程建梅, 李  华. 西北半干旱区葡萄园生草体系中土壤生物学特性与土壤养分的关系. 中国农业科学, 2011, 44(11): 2310-2317.

Xi Z M, Yue T X, Zhang J, Cheng J M, Li H. Relationship between soil biological characteristics and nutrient content under intercropping system of vineyard in northwestern semiarid area. Scientia Agricultura Sinica, 2011, 44(11): 2310-2317. (in Chinese)

[9]夏  雪, 谷  洁, 车升国, 高  华, 秦清军. 施氮水平对塿土微生物群落和酶活性的影响. 中国农业科学, 2011, 44(8): 1618-1627.

Xia X, Gu J, Che S G, Gao H, Qin Q J. Effects of nitrogen  application rates on microbial community and enzyme activities in Lou soil. Scientia Agricultura Sinica, 2011, 44(8): 1618-1627. (in Chinese)

[10]朱丽霞, 章家恩, 刘文高. 根系分泌物与根际微生物相互作用研究综述. 生态环境, 2003, 12(1): 102-105.

Zhu L X, Zhang J E, Liu W G. Review of studies on interactions between root exudates and rhizopheric microorganisms. Ecology and Environment, 2003, 12 (1): 102-105. (in Chinese)

[11]Nihorimbere V, Ongena M, Smargiassi M, Thonart P. Beneficial effect of the rhizosphere microbial community for plant growth and health. Biotechnology, Agronomy, Society and Environment, 2011, 15(2): 327-337.

[12]Richardson A E, Barea J M, McNeill A M, Prigent-Combaret C. Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant and Soil, 2009, 321(1-2): 305-339.

[13]Morgan J A, Bending G D, White P J. Biological costs and benefits to plant-microbe interactions in the rhizosphere. Journal of Experimental Botany, 2005, 56(417): 1729-1739.

[14]Rumberger A, Yao S G, Merwin I A, Nelson E B, Thies J E. Rootstock genotype and orchard replant position rather than soil fumigation or compost amendment determine tree growth and rhizosphere bacterial community composition in an apple replant soil. Plant and Soil, 2004, 264(1/2): 247-260.

[15]殷瑞敬, 温晓霞, 廖允成, 黄金辉, 高茂盛. 耕作和覆盖对苹果园土壤酶活性的影响. 园艺学报, 2009, 36(5): 717-722.

Yin R J, Wen X X, Liao Y C, Huang J H, Gao M S. Effect of tillage and mulching on enzyme activities of apple orchard soil. Acta Horticulturae Sinica, 2009, 36(5): 717-722. (in Chinese)

[16]谭向平, 和文祥, 杨  静, 孔  龙, 王旭东, 郝明德. 渭北旱塬苹果园土壤酶特征研究. 园艺学报, 2011, 38(4): 621-630.

Tan X P, He W X, Yang J, Kong L, Wang X D, Hao M D. Characteristics of enzyme activities in apple orchard soil in Weibei arid region. Acta Horticulturae Sinica, 2011, 38(4): 621-630. (in Chinese)

[17]刘灵芝, 吕德国, 秦嗣军, 马怀宇, 杜国栋, 刘国成. 生草覆盖‘寒富’苹果园土壤优势细菌的碳代谢特征研究. 园艺学报, 2011, 38(10):1837-1846.

Liu L Z, Lü D G, Qin S J, Ma H Y, Du G D, Liu G C. Carbon catabolic characteristics about the soil dominant bacteria from ‘Hanfu’ apple orchard with herbage-mulching management. Acta Horticulturae Sinica, 2011, 38(10):1837-1846.(in Chinese)

[18]林先贵. 土壤微生物研究原理与方法. 北京: 高等教育出版社, 2010: 52-62.

Lin X G. Principles and Methods of Soil Microbiology. Beijing: Higher Education Press, 2010: 52-62. (in Chinese)

[19]严  君, 韩晓增, 王树起, 李晓慧, 朱巍巍. 不同形态氮素对种植大豆土壤中微生物数量及酶活性的影响. 植物营养与肥料学报, 2010, 16(2): 341-347.

Yan J, Han X Z, Wang S Q, Li X H, Zhu W W. Effects of different nitrogen forms on microbial quantity and enzymes activities in soybean field. Plant Nutrition and Fertilizer Science, 2010, 16(2): 341-347. (in Chinese)

[20]关松荫. 土壤酶及其研究方法. 北京: 农业出版社, 1986: 274-332.

Guan S Y. Soil Enzymes and the Research Methods. Beijing: Agriculture Press, 1986: 274-332. (in Chinese)

[21]Susanne K, Veronica A M, Husein A. Microbial community composition and enzyme activities in a sandy loam soil after fumigation with methyl bromide or alternative biocides. Soil Biology & Biochemistry, 2006, 38(6): 1243-1254.

[22]Marschner P, Yang C H, Lieberei R, Crowley D E. Soil and plant specific effects on bacterial community composition in the rhizosphere. Soil Biology and Biochemistry, 2001, 33(11): 1437-1445.

[23]Xu Y X, Wang G H, Jin J, Liu J J, Zhang Q Y, Liu X B. Bacterial communities in soybean rhizosphere in response to soil type, soybean genotype, and their growth stage. Soil Biology and Biochemistry, 2009,41(5): 919-925.

[24]许  衡, 杨和生, 徐  英, 毛志泉, 束怀瑞. 果树根际微域环境的研究进展. 山东农业大学学报: 自然科学版, 2004, 35(3): 476-480.

Xu H, Yang H S, Xu Y, Mao Z Q, Shu H R. Research progress on rhizosphere environment of fruit trees. Journal of Shandong Agricultural University: Natural Science Edition, 2004, 35(3): 476-480. (in Chinese)

[25]张江红, 张殿生, 毛志泉, 李中勇, 赵明新. 苹果砧木幼苗根系分泌物的分离与鉴定. 河北农业大学学报: 自然科学版, 2009, 32(4): 29-32.

Zhang J H, Zhang D S, Mao Z Q, Li Z Y, Zhao M X. Isolation and identification of root exudates in different kinds of apple rootstock seedlings Journal of Agricultural University of Hebei: Natural Science Edition, 2009, 32(4): 29-32. (in Chinese)

[26]Walker T S, Bais H P, Grotewold E, Vivanco J M. Root exudation and rhizosphere biology. Plant Physiology, 2003, 132(1): 44-51.

[27]董淑富, 何承顺, 黄天栋, 束怀瑞. 苹果砧木苗根际微域环境的研究. 土壤学报, 1997, 34(3): 323-326.

Dong S F, He C S, Huang T D, Shu H R. Study on rhizosphere of apple rootstock seedlings. Acta Pedologica Sinica, 1997, 34(3): 323-326. (in Chinese)

[28]刘素慧, 刘世琦, 张自坤, 尉  辉, 齐建建, 段吉锋. 大蒜连作对其根际土壤微生物和酶活性的影响. 中国农业科学, 2010, 43(5): 1000-1006.

Liu S H, Liu S Q, Zhang Z K, Wei H, Qi J J, Duan J F. Influence of garlic continuous cropping on rhizosphere soil microorganisms and enzyme activities. Scientia Agricultura Sinica, 2010, 43(5): 1000-1006. (in Chinese)

[29]黄继川, 彭智平, 于俊红, 林志军, 杨林香. 施用玉米秸秆堆肥对盆栽芥菜土壤酶活性和微生物的影响. 植物营养与肥料学报, 2010, 16(2): 348-353.

Huang J C, Peng Z P, Yu J H, Lin Z J, Yang L X. Impacts of applying corn-straw compost on microorganisms and enzyme activities in pot soil cultivated with mustard. Plant Nutrition and Fertilizer Science, 2010, 16(2): 348-353. (in Chinese)
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