Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (2): 385-390.doi: 10.3864/j.issn.0578-1752.2012.02.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Study of Class I Integrons in qnr-positive Enterobacteriaceae Isolates from Animals

 YUE  Lei, CHEN  Xue-Ying, ZHUANG  Na, HE  Jia-Xin, SHI  Wei, LIAO  Xiao-Ping, LIU  Ya-Hong   

  1. 1.华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室，广州 510642
  • Received:2011-02-25 Online:2012-01-15 Published:2011-11-18

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Abstract: 【Objective】The objective of this study is to investigate the prevalence of class 1 integrons in qnr-positive enterobacteriaceae isolates from animals. 【Method】 Fourteen qnr-positive strains of Enterobacteriaceae from animals were screened for class 1 integrase gene intI by PCR and sequencing. Gene cassettes of the detected class 1 integrons were studied by PCR using LA Taq enzyme and sequencing.【Result】intI1 was detected in all the 14 qnr positive strains tested. And more than two resistance genes were found in each cassette. The genes most commonly found in integrons were dihydrofolate reductase (dfr) gene for trimethoprim resistance and adenylvltransferase (aadA) gene for amino glycoside resistance. Lincosamide nucleotidyltransferase linF gene and ribosyl transferase arr-3 gene for rifampin resistance were also detected in this study. Plasmid-mediated quinolone resistance aac (6')-Ib-cr was found following an empty cassette. dfrA27-aadA2, dfrA12-orfF-aadA2 and dfrA17-aadA5 were the major arrays.【Conclusion】It seems that the class 1 integrons are extensively existed in qnr positive strains.

Key words: qnr gene, animal, enterobacteriaceae, integron

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